FastQCFastQC Report
Thu 26 May 2016
SRR937726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787507
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100760.5636901002345724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87870.49157849451778374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61180.34226439392964614No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21750.12167784517766925No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20910.11697856288115235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67600.036.4107741
GTATCAA102900.034.587031
ATCAACG127750.027.6669733
TCAACGC128400.027.5269154
TATCAAC129450.027.3403362
CAACGCA129500.027.2930955
AACGCAG132450.026.716596
GTAGGAC3950.024.0537363
TACATGG81400.023.8697852
GTACATG81550.023.714681
CGCAGAG150800.023.2772678
ACGCAGA151400.023.2477657
GAGTACT87000.022.8212712-13
ACATGGG81750.022.6634733
GTACTTT92000.021.52845214-15
GCAGAGT162300.021.4230589
AGAGTAC129650.021.23096810-11
AGTACTT91750.020.83735512-13
GCGTACA1404.8489662E-420.359772
GTGTAGG8450.020.243831
Produced by FastQC (version 0.11.5)