##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937726_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1787507 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.207983521183415 33.0 31.0 34.0 31.0 34.0 2 32.428236085229315 34.0 31.0 34.0 31.0 34.0 3 32.50854626023842 34.0 31.0 34.0 31.0 34.0 4 36.136453731370004 37.0 35.0 37.0 35.0 37.0 5 36.0492859608382 37.0 35.0 37.0 35.0 37.0 6 36.08768748877627 37.0 35.0 37.0 35.0 37.0 7 36.02852967848518 37.0 35.0 37.0 35.0 37.0 8 36.006370324703624 37.0 35.0 37.0 35.0 37.0 9 37.684485711104905 39.0 37.0 39.0 35.0 39.0 10-11 37.617329330738286 39.0 37.0 39.0 35.0 39.0 12-13 37.51482539648796 39.0 37.0 39.0 35.0 39.0 14-15 38.72004473269196 40.0 38.0 41.0 34.5 41.0 16-17 38.68104320710353 40.0 38.0 41.0 34.5 41.0 18-19 38.67408519239365 40.0 38.0 41.0 34.0 41.0 20-21 38.57214097623114 40.0 38.0 41.0 34.0 41.0 22-23 38.41560676405743 40.0 38.0 41.0 34.0 41.0 24-25 38.49096842697679 40.0 38.0 41.0 34.0 41.0 26-27 38.36374542868923 40.0 38.0 41.0 34.0 41.0 28-29 38.22366262062191 40.0 38.0 41.0 34.0 41.0 30-31 38.25970080117169 40.0 38.0 41.0 34.0 41.0 32-33 38.183253548097994 40.0 37.5 41.0 34.0 41.0 34-35 38.05171700026909 40.0 37.0 41.0 33.5 41.0 36-37 37.888769386637364 40.0 37.0 41.0 33.0 41.0 38-39 37.75104181410198 40.0 37.0 41.0 33.0 41.0 40-41 37.573664606628114 40.0 37.0 41.0 33.0 41.0 42-43 37.34417571511608 40.0 36.5 41.0 32.5 41.0 44-45 37.02123544131575 40.0 36.0 41.0 31.5 41.0 46-47 36.64223608634819 39.5 36.0 41.0 31.0 41.0 48-49 36.29301004135928 39.0 36.0 41.0 30.0 41.0 50-51 36.02511934218999 39.0 35.0 41.0 29.5 41.0 52-53 35.773160328882625 39.0 35.0 40.0 28.5 41.0 54-55 35.54614191720648 39.0 35.0 40.0 28.0 41.0 56-57 35.26727391836787 38.0 35.0 40.0 27.5 41.0 58-59 34.993649255639276 38.0 34.0 40.0 27.0 41.0 60-61 34.628656279388 38.0 34.0 40.0 26.0 41.0 62-63 34.26716398872844 37.0 34.0 40.0 26.0 41.0 64-65 33.86325256348646 37.0 33.0 39.5 25.5 41.0 66-67 33.4225326110611 36.0 33.0 39.0 24.5 40.5 68-69 32.96752488242004 36.0 32.0 39.0 22.5 40.0 70-71 32.539605159588184 35.0 32.0 38.0 22.0 40.0 72-73 32.02856660141751 35.0 31.0 37.5 20.5 39.5 74-75 31.515517981188324 35.0 31.0 37.0 19.5 39.0 76-77 30.301155464006573 33.5 29.5 35.5 18.5 38.0 78-79 30.902424997496514 34.0 31.0 36.0 18.5 38.0 80-81 30.699600896667818 34.0 31.0 35.5 18.0 37.0 82-83 30.341964534964056 34.0 30.0 35.0 15.5 37.0 84-85 29.94563713596646 34.0 30.0 35.0 9.5 36.5 86-87 29.519495308270123 34.0 29.5 35.0 7.0 36.0 88-89 29.13721876333911 34.0 29.0 35.0 2.0 36.0 90-91 28.59476997852316 34.0 29.0 35.0 2.0 35.0 92-93 28.095009138425752 33.0 28.0 35.0 2.0 35.0 94-95 27.592679357339577 33.0 27.0 35.0 2.0 35.0 96-97 27.007326125156432 33.0 26.0 35.0 2.0 35.0 98-99 26.105792592700336 32.5 24.5 34.0 2.0 35.0 100-101 23.455901711154137 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25613833368979044 1101 2 0.24405188290857893 1101 3 0.18245644498257718 1101 4 0.07738162028683604 1101 5 0.12286410985036866 1101 6 0.13818427518748422 1101 7 0.16686647796912268 1101 8 0.10169014259891185 1101 9 0.23813548426786468 1101 10-11 0.23636015922408404 1101 12-13 0.19926515081120044 1101 14-15 0.228608312600457 1101 16-17 0.22726122418428218 1101 18-19 0.320022844129916 1101 20-21 0.37296553705714075 1101 22-23 0.28989405447788386 1101 24-25 0.23707670076274923 1101 26-27 0.1646293479375771 1101 28-29 0.19582106599661842 1101 30-31 0.3226599666139691 1101 32-33 0.32876224878416593 1101 34-35 0.3577832021973393 1101 36-37 0.21215712508229245 1101 38-39 0.1279670613441226 1101 40-41 0.16228744284640584 1101 42-43 0.23619005207380894 1101 44-45 0.25913885122525926 1101 46-47 0.32179346133587217 1101 48-49 0.23286876298447368 1101 50-51 0.11152015503296298 1101 52-53 0.12875627101982445 1101 54-55 0.3053148102002936 1101 56-57 0.18968843581703254 1101 58-59 0.28905854236025874 1101 60-61 0.1212752629330609 1101 62-63 0.26237028668653295 1101 64-65 0.4136017843347872 1101 66-67 0.2082839876565643 1101 68-69 0.2762691118738658 1101 70-71 0.4132523913801194 1101 72-73 0.40515350547352824 1101 74-75 0.2957607378599967 1101 76-77 0.18460329360186734 1101 78-79 0.34113135314764165 1101 80-81 0.3275055650477725 1101 82-83 0.364277447943401 1101 84-85 0.30366337605640226 1101 86-87 0.18886446247094213 1101 88-89 0.2873711709431781 1101 90-91 0.14040486545738062 1101 92-93 0.185105364380032 1101 94-95 0.29554307590145257 1101 96-97 0.5496363823890498 1101 98-99 0.7245116865958714 1101 100-101 0.9458695214915416 1102 1 0.13155858961865619 1102 2 0.1330845034727588 1102 3 0.08799501299522205 1102 4 0.04835757172880051 1102 5 -0.010631086736111683 1102 6 0.04771186895647617 1102 7 0.06790651187973396 1102 8 0.05924196443327645 1102 9 0.16712016387085527 1102 10-11 0.20745570359719068 1102 12-13 0.17741785753889872 1102 14-15 0.162560462690287 1102 16-17 0.20858892273273 1102 18-19 0.1844679128793345 1102 20-21 0.1254597101451509 1102 22-23 0.21417616075707713 1102 24-25 0.10889548751487155 1102 26-27 0.19541591795805857 1102 28-29 0.08816136284655585 1102 30-31 0.14603842681842139 1102 32-33 0.06696742543613254 1102 34-35 0.15494981685169762 1102 36-37 0.0921318195011267 1102 38-39 0.08146947124783566 1102 40-41 -0.03784083059728971 1102 42-43 0.12560503978073712 1102 44-45 -0.004364240381747209 1102 46-47 -0.0014376896269681083 1102 48-49 -0.23882710487925607 1102 50-51 -0.2571348443643444 1102 52-53 -0.2586494610905845 1102 54-55 -0.17394607717925226 1102 56-57 -0.23876951594525764 1102 58-59 -0.13515362446315038 1102 60-61 -0.24530667438279607 1102 62-63 -0.1111882587709303 1102 64-65 -0.07795877603948043 1102 66-67 -0.10344005884928009 1102 68-69 -0.1326934229867689 1102 70-71 -0.32981151346825754 1102 72-73 -0.13410151784238167 1102 74-75 -0.13478442899336684 1102 76-77 -0.17393077068723528 1102 78-79 -0.032545016114756464 1102 80-81 -0.23707968926176193 1102 82-83 -0.2148897701009318 1102 84-85 -0.02967656201474611 1102 86-87 0.002753919445908082 1102 88-89 0.07392692047359262 1102 90-91 -0.06273316341994928 1102 92-93 -0.05207666761944907 1102 94-95 -0.0680302853982262 1102 96-97 0.19759477537141734 1102 98-99 0.18181701017966745 1102 100-101 0.4819448353168383 1103 1 0.2578150867312843 1103 2 0.24408859001771077 1103 3 0.18781014221957548 1103 4 0.09054862193472957 1103 5 0.09570939100143505 1103 6 0.11424431416627812 1103 7 0.2192876927389591 1103 8 0.16936650871515724 1103 9 0.3009194160655966 1103 10-11 0.18805798999553502 1103 12-13 0.22544459178270415 1103 14-15 0.23635559038739018 1103 16-17 0.2680335879328055 1103 18-19 0.20637324788502553 1103 20-21 0.21974129078799365 1103 22-23 0.3653926132044063 1103 24-25 0.39073208241740787 1103 26-27 0.25238554162736904 1103 28-29 0.27609270322140844 1103 30-31 0.3507359415570974 1103 32-33 0.20039240919192025 1103 34-35 0.30343013152068465 1103 36-37 0.23139545949375417 1103 38-39 0.25312722207970495 1103 40-41 0.22604688338388712 1103 42-43 0.42133609326980803 1103 44-45 0.535458310194791 1103 46-47 0.5748743190170984 1103 48-49 0.3881781577419545 1103 50-51 0.44421723180808215 1103 52-53 0.29815646300494336 1103 54-55 0.2992622927159374 1103 56-57 0.32571805637633844 1103 58-59 0.24376116167798756 1103 60-61 0.16367659122251155 1103 62-63 0.3081834137951702 1103 64-65 0.42956626016419364 1103 66-67 0.2568362962105297 1103 68-69 0.3394354671072577 1103 70-71 0.27332927776769367 1103 72-73 0.3199072469437354 1103 74-75 0.24261819376645022 1103 76-77 0.17455235613923747 1103 78-79 0.3945522017698302 1103 80-81 0.27362079859040733 1103 82-83 0.3790171991569302 1103 84-85 0.41874801249210947 1103 86-87 0.4970107165219986 1103 88-89 0.3900781240084079 1103 90-91 0.3747587487694055 1103 92-93 0.3983876355850846 1103 94-95 0.391210921351135 1103 96-97 0.5368214457815306 1103 98-99 0.8350416402895071 1103 100-101 1.0470187310420371 1104 1 0.2029791941470478 1104 2 0.2449625586119879 1104 3 0.10881677545145152 1104 4 0.01170080498103232 1104 5 0.09917111028406111 1104 6 0.07841271798039884 1104 7 0.048830331467797805 1104 8 0.04157287870724957 1104 9 0.15141654011734573 1104 10-11 0.11964868427261877 1104 12-13 0.14985166603945288 1104 14-15 0.19119184014908797 1104 16-17 0.20969847243277684 1104 18-19 0.14535505996236253 1104 20-21 0.15679590723967607 1104 22-23 0.10664198870194497 1104 24-25 0.06802325303527823 1104 26-27 0.17687141816094254 1104 28-29 -0.036773431578261295 1104 30-31 0.0845736615505075 1104 32-33 0.14575742892821353 1104 34-35 0.1471565103707846 1104 36-37 0.13940429338525462 1104 38-39 0.1682160945285105 1104 40-41 0.33466644608259344 1104 42-43 0.298341365655574 1104 44-45 0.36288701645785437 1104 46-47 0.4996233906905587 1104 48-49 0.4383734221939122 1104 50-51 0.39579803226955335 1104 52-53 0.4330750019883993 1104 54-55 0.47387652989873885 1104 56-57 0.41718807824899073 1104 58-59 0.31693376366666115 1104 60-61 0.4514531302890177 1104 62-63 0.4666533414622762 1104 64-65 0.3952507706725754 1104 66-67 0.3086380779009872 1104 68-69 0.30411139776661145 1104 70-71 0.23774636638743374 1104 72-73 0.1999787761945626 1104 74-75 0.212092580347651 1104 76-77 0.02694665058650969 1104 78-79 0.2834496976433165 1104 80-81 0.3374422714741634 1104 82-83 0.34290443734026965 1104 84-85 0.38866518750415224 1104 86-87 0.3160614906371677 1104 88-89 0.4558909728424254 1104 90-91 0.5034185341146831 1104 92-93 0.452187155449284 1104 94-95 0.3270124030125281 1104 96-97 0.45899962107797876 1104 98-99 0.5094699219578622 1104 100-101 0.9981297315273494 1105 1 0.20541416924389466 1105 2 0.21399686396619444 1105 3 0.1938745433004101 1105 4 0.08779343724614819 1105 5 0.0711793083504304 1105 6 0.08085221989467328 1105 7 0.10032129537434997 1105 8 0.09100165093250467 1105 9 0.18620490574386395 1105 10-11 0.20051077138442963 1105 12-13 0.1929995922478014 1105 14-15 0.18444744802710034 1105 16-17 0.23362123134693036 1105 18-19 0.22816017028656432 1105 20-21 0.3214253928913635 1105 22-23 0.32126643826923384 1105 24-25 0.24087887132498054 1105 26-27 0.2281570908969428 1105 28-29 0.2318660221254092 1105 30-31 0.2514014829177711 1105 32-33 0.28349784537278566 1105 34-35 0.16939370881400606 1105 36-37 0.1304313490413378 1105 38-39 0.10136450736973046 1105 40-41 0.16477295077973508 1105 42-43 0.17218971500817304 1105 44-45 0.14578718994813755 1105 46-47 0.11230787264722863 1105 48-49 0.123041544232386 1105 50-51 0.14838109088165652 1105 52-53 0.2195589436694121 1105 54-55 0.15137553116964142 1105 56-57 0.12644474087547763 1105 58-59 0.18812283411865138 1105 60-61 0.16773835600179865 1105 62-63 0.16462125311690556 1105 64-65 0.20320857620728816 1105 66-67 0.16510763561960573 1105 68-69 0.025689604762128226 1105 70-71 0.19161277774159657 1105 72-73 0.09925731466628207 1105 74-75 0.03679847867759278 1105 76-77 0.11949415104768235 1105 78-79 0.23880735583012225 1105 80-81 0.05809146236213536 1105 82-83 0.30728245828359135 1105 84-85 0.28307603919089885 1105 86-87 0.26660408629165033 1105 88-89 0.32760662533222984 1105 90-91 0.3187613896084862 1105 92-93 0.41900784707908656 1105 94-95 0.6699699765478506 1105 96-97 0.5365076759837457 1105 98-99 0.6030711523468426 1105 100-101 0.7760621755674002 1106 1 0.0037488021751101996 1106 2 0.03527098738447876 1106 3 0.019984817415597433 1106 4 0.02540779892012779 1106 5 0.004473124499078551 1106 6 -0.011843334304437292 1106 7 0.030463380823206876 1106 8 0.024648769529932224 1106 9 -0.04584423669794546 1106 10-11 -0.05966649141544167 1106 12-13 -0.13540820628041672 1106 14-15 0.028179916686802642 1106 16-17 0.0724385149403588 1106 18-19 0.0442658928688644 1106 20-21 0.1256848984796335 1106 22-23 0.07647088677516933 1106 24-25 0.17091851788139678 1106 26-27 0.05827882970011444 1106 28-29 0.222120214063672 1106 30-31 0.21437893812868936 1106 32-33 0.20827512016892769 1106 34-35 0.07864202374715745 1106 36-37 0.03862410822291196 1106 38-39 0.19226414665781277 1106 40-41 0.11227895774463548 1106 42-43 0.05958396831567825 1106 44-45 0.1866003265893852 1106 46-47 0.2081998496002413 1106 48-49 0.20820930997075493 1106 50-51 0.32823109023479446 1106 52-53 0.2342105279439295 1106 54-55 0.3178432005848677 1106 56-57 0.1970399567087 1106 58-59 0.09599698599871687 1106 60-61 0.10924927674043516 1106 62-63 0.09191727799391458 1106 64-65 0.08072753210682038 1106 66-67 0.11239312258069845 1106 68-69 0.18410223708882967 1106 70-71 0.2694071778272118 1106 72-73 0.19186724829858548 1106 74-75 0.039948562828026724 1106 76-77 0.04220677055482014 1106 78-79 0.08577000150563663 1106 80-81 0.17035178652065497 1106 82-83 0.07045620570056954 1106 84-85 0.051500632522611056 1106 86-87 0.10808042949369678 1106 88-89 -0.13928009089557136 1106 90-91 -0.01756654657126333 1106 92-93 0.21323916139007082 1106 94-95 0.29339331732593976 1106 96-97 0.16853635416920199 1106 98-99 0.32807600378692925 1106 100-101 0.36356451307944226 1107 1 0.17717484987136345 1107 2 0.23124930405234068 1107 3 0.21742767531709006 1107 4 0.0393873213745195 1107 5 0.07531256225685468 1107 6 0.07371830918884115 1107 7 0.13497815587321327 1107 8 0.1839929903450539 1107 9 0.14733307327353629 1107 10-11 0.1618523987195033 1107 12-13 0.20731552803420072 1107 14-15 0.2736652457634392 1107 16-17 0.2710362674708975 1107 18-19 0.2831709387418542 1107 20-21 0.2070227748584017 1107 22-23 0.18543421233024304 1107 24-25 0.20029706925071622 1107 26-27 0.26972294649272044 1107 28-29 0.26060099592981345 1107 30-31 0.3018516959957154 1107 32-33 0.3192831025173817 1107 34-35 0.3470155603773435 1107 36-37 0.4027048678059515 1107 38-39 0.44295060155170773 1107 40-41 0.4165547100856628 1107 42-43 0.5310962203771368 1107 44-45 0.5767079310622449 1107 46-47 0.5674480916821949 1107 48-49 0.8495724214414437 1107 50-51 0.7569099365948944 1107 52-53 0.5537033478912576 1107 54-55 0.5259883250109993 1107 56-57 0.5709681030774405 1107 58-59 0.47575362120873166 1107 60-61 0.755114824253944 1107 62-63 0.6615816465855673 1107 64-65 0.6057170153158751 1107 66-67 0.5767264933996259 1107 68-69 0.5563560270383263 1107 70-71 0.5715823053042541 1107 72-73 0.7543062691332878 1107 74-75 0.5414258595754227 1107 76-77 0.42705689066490393 1107 78-79 0.625499912612721 1107 80-81 0.5321482678731364 1107 82-83 0.5727833060130507 1107 84-85 0.5470883961842894 1107 86-87 0.7578094659369796 1107 88-89 0.6875613367223536 1107 90-91 0.8041728056985384 1107 92-93 1.0374245786619731 1107 94-95 1.1370635628253218 1107 96-97 1.2667070833368435 1107 98-99 1.3933100440360384 1107 100-101 1.5198989532789895 1108 1 0.008155682673383069 1108 2 0.06888678750803479 1108 3 -0.042601488956044875 1108 4 -0.05231180264518542 1108 5 -0.16109901170908358 1108 6 -0.05681548538609604 1108 7 -0.0938819545133498 1108 8 -0.05349856653474916 1108 9 0.0814420163526961 1108 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2316 54-55 0.152415355048241 2316 56-57 0.06376660469589268 2316 58-59 0.13935086808492514 2316 60-61 0.2420213885921143 2316 62-63 0.18884531648915726 2316 64-65 0.14362902673211408 2316 66-67 0.054078917610404176 2316 68-69 0.19511947070544267 2316 70-71 0.33243077907822993 2316 72-73 0.4593852778694938 2316 74-75 0.6467443744200132 2316 76-77 0.6326764740417445 2316 78-79 0.4315445595097991 2316 80-81 0.4056896952512332 2316 82-83 0.5089574297818054 2316 84-85 0.549154979317489 2316 86-87 0.5225115734167041 2316 88-89 0.6387355910121464 2316 90-91 0.7035421715403167 2316 92-93 0.6363290720663102 2316 94-95 0.7137968822364122 2316 96-97 0.7300100052678609 2316 98-99 0.9602064277330911 2316 100-101 1.1892336875381524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 14.0 5 37.0 6 71.0 7 139.0 8 398.0 9 813.0 10 1360.0 11 2294.0 12 3569.0 13 5385.0 14 6809.0 15 9487.0 16 13746.0 17 19491.0 18 17696.0 19 10890.0 20 9098.0 21 9425.0 22 9746.0 23 10434.0 24 12039.0 25 14403.0 26 17450.0 27 21511.0 28 27178.0 29 33958.0 30 42766.0 31 53908.0 32 68719.0 33 91019.0 34 124612.0 35 177552.0 36 268467.0 37 389753.0 38 288175.0 39 25086.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.173348523440964 19.68040341822186 15.313508601510357 21.83273945682682 2 15.795910169862271 20.69882803256155 40.08431855092036 23.420943246655817 3 19.67332155902047 24.604043508640807 30.120889037077898 25.60174589526083 4 13.132789147766466 16.87874090281247 36.47814856814227 33.5103213812788 5 13.478857429361554 37.997647530186484 33.92094716567677 14.60254787477519 6 32.27355193574067 36.21792809762424 16.863654240235142 14.644865726399953 7 28.811467591455585 33.34414914179357 19.895754254388933 17.94862901236191 8 26.847894861390753 35.52976296036883 18.90773015154626 18.714612026694162 9 26.687559824940543 16.85710881132214 20.234829849617373 36.220501514119945 10-11 25.4048937458112 27.15828646169579 26.298876759015076 21.137943033477928 12-13 26.106212885086677 24.376503023740398 27.628139373746286 21.889144717426642 14-15 22.97806386212753 25.58222149619554 26.28073624327066 25.158978398406273 16-17 21.876417826615505 29.132529271214043 26.913321178602377 22.077731723568075 18-19 21.75482389719313 27.219166134734017 29.89045637303798 21.13555359503487 20-21 22.530177504199983 26.085016730004412 30.35806293345984 21.026742832335763 22-23 22.38239387924533 25.975444925068196 30.25182770048761 21.39033349519886 24-25 22.21130384700699 26.167065686194707 30.341036522104954 21.28059394469335 26-27 21.76527091763408 26.46804277932463 30.397191830596825 21.36949447244446 28-29 21.730913879764486 26.7416174750704 30.2827970160284 21.244671629136715 30-31 21.818208264359246 26.366889752040134 30.31714001679434 21.497761966806284 32-33 21.792418155565265 26.35886181145025 30.244413028872053 21.60430700411243 34-35 22.254710051485112 26.440903448210275 30.118231704826893 21.186154795477723 36-37 22.201143827688508 26.313910938530586 30.071434685290743 21.413510548490162 38-39 21.917533097809518 26.67830299775385 30.098517206944877 21.305646697491756 40-41 22.15505799325032 26.50089440934717 30.025608499056638 21.318439098345866 42-43 21.89177265246162 26.534351930936644 29.769192915175648 21.80468250142609 44-45 22.009955765208193 26.76957348978214 29.625814052756155 21.594656692253515 46-47 22.272360332015484 27.080313531639316 29.029872330569894 21.617453805775305 48-49 22.375856430212583 27.11737632356125 28.820810212211757 21.68595703401441 50-51 22.371436867100382 27.33206079752415 28.63015361618164 21.666348719193827 52-53 22.570317207149397 27.469319001268243 28.27893820835387 21.681425583228485 54-55 22.340276149967526 27.418745772743826 28.334154775337943 21.906823301950705 56-57 22.36872359101251 27.73228300644417 28.25737185924307 21.64162154330025 58-59 22.58223324440128 27.255697460205752 28.367217582924155 21.79485171246882 60-61 22.438010032967703 27.37144525867591 28.720251165449984 21.470293542906404 62-63 22.24111569912733 27.351137645894536 28.859886982260768 21.547859672717365 64-65 22.2261507227664 27.29164137539042 28.666628997816513 21.81557890402667 66-67 22.313982546641775 27.50621396167959 28.307749284338467 21.872054207340167 68-69 22.244630855681283 27.395020602733812 28.517366799710548 21.84298174187436 70-71 22.35977883157348 27.413746467495365 28.343085745422464 21.883388955508693 72-73 22.421926179869505 27.38344521168309 28.26573546285413 21.928893145593275 74-75 22.194654342612363 27.868646108798455 27.93558290960539 22.00111663898379 76-77 22.332136321703917 27.553430559992215 28.097204654303454 22.01722846400042 78-79 22.427156928616224 27.57695494339323 28.03216994394987 21.96371818404068 80-81 22.36174312142169 27.8868994006174 27.914060148609295 21.83729732935162 82-83 22.443876601952205 27.814212006651868 27.87440890533674 21.867502486059188 84-85 22.33062339719921 27.747418735482487 27.852537851832952 22.06942001548535 86-87 22.36212221826264 27.768198949710403 27.931946560209276 21.93773227181768 88-89 22.339800627354187 27.67259093251103 27.85787692020227 22.12973151993251 90-91 22.425592673740184 27.615138609451893 27.86679975203153 22.092468964776394 92-93 22.40500731062652 27.549187635124913 27.923935917120684 22.121869137127884 94-95 22.364835494350512 27.517542588644407 27.848282552180216 22.269339364824862 96-97 22.300947632652626 27.522074039430333 27.858240555141883 22.318737772775155 98-99 22.128629798903876 27.625898717103453 27.65034656360902 22.595124920383657 100-101 22.178997688816374 27.583631079027036 27.9479481495843 22.289423082572288 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 461.0 1 342.0 2 305.0 3 410.0 4 360.0 5 331.0 6 641.5 7 1562.5 8 2578.5 9 3117.5 10 2930.5 11 2583.5 12 2721.5 13 2932.5 14 3143.5 15 3341.5 16 3444.5 17 3558.0 18 3708.0 19 3900.5 20 4231.5 21 4694.5 22 5451.0 23 6395.5 24 7465.0 25 9095.0 26 11273.0 27 13598.0 28 15568.0 29 17740.5 30 20503.0 31 23306.5 32 26833.5 33 30570.5 34 34432.5 35 37700.5 36 42188.5 37 47168.5 38 49990.5 39 51820.5 40 52680.5 41 55077.0 42 57676.0 43 59861.0 44 63040.5 45 65421.0 46 66209.5 47 68433.0 48 72582.0 49 72095.5 50 68559.0 51 65603.5 52 62667.0 53 61446.0 54 60725.5 55 55749.5 56 48479.5 57 42314.0 58 37735.5 59 33358.0 60 27415.5 61 22046.0 62 17570.0 63 13297.0 64 9692.5 65 7106.5 66 5087.0 67 4040.0 68 3061.5 69 1981.5 70 1391.0 71 936.5 72 653.0 73 483.5 74 323.5 75 221.5 76 130.0 77 64.5 78 41.0 79 26.0 80 18.5 81 13.0 82 9.0 83 7.5 84 4.5 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021202714171189258 2 0.0 3 0.0 4 2.2377534745318483E-4 5 0.02511878275161999 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.5943836863296207E-5 12-13 0.00383215282513579 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.39157552949443E-4 24-25 2.7971918431648103E-5 26-27 4.4755069490636965E-4 28-29 0.0013146801662874607 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.1188767372659241E-4 40-41 8.111856345177949E-4 42-43 0.015580358566427992 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.391575529494429E-5 70-71 2.7971918431648103E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.5943836863296207E-5 82-83 0.0022097815561002 84-85 6.153822054962582E-4 86-87 0.0 88-89 0.0 90-91 0.010237722145983204 92-93 0.002265725392963496 94-95 0.0 96-97 0.0 98-99 0.0015943993506039417 100-101 0.018321606572729503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1787507.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.10855142254888 #Duplication Level Percentage of deduplicated Percentage of total 1 76.73870907953568 42.28959095409611 2 13.231516126065557 14.58339373663137 3 4.275988825112113 7.0693065015280565 4 1.8787937932947338 4.141504174805942 5 0.9863381595072046 2.7177833591612504 6 0.6114643806980132 2.0218149760052078 7 0.3999710231716188 1.5429276588587848 8 0.2863427875644943 1.2623948986379139 9 0.2222725730171745 1.1024207567945292 >10 1.2249397973696423 12.74365388391859 >50 0.09354444568886043 3.550682014247137 >100 0.04798874468046813 4.717956593999826 >500 0.0015216173531540907 0.5325296165156179 >1k 3.0432347063081807E-4 0.3275342245606766 >5k 2.0288231375387874E-4 0.8332305200677393 >10k+ 1.0144115687693937E-4 0.563276130171254 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10076 0.5636901002345724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8787 0.49157849451778374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6118 0.34226439392964614 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2175 0.12167784517766925 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2091 0.11697856288115235 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.59438368632962E-5 0.0 18-19 0.0 0.0 0.0 5.59438368632962E-5 0.0 20-21 0.0 0.0 0.0 1.118876737265924E-4 0.0 22-23 0.0 2.79719184316481E-5 0.0 2.2377534745318483E-4 0.0 24-25 0.0 5.59438368632962E-5 0.0 3.356630211797772E-4 0.0 26-27 0.0 5.59438368632962E-5 0.0 5.314664502013139E-4 0.0 28-29 0.0 5.59438368632962E-5 0.0 0.0013146801662874607 0.0 30-31 0.0 5.59438368632962E-5 0.0 0.0029370514353230506 0.0 32-33 0.0 1.118876737265924E-4 0.0 0.005062917236128306 0.0 34-35 0.0 1.118876737265924E-4 0.0 0.006153822054962582 0.0 36-37 0.0 1.118876737265924E-4 0.0 0.0088391262244008 0.0 38-39 0.0 1.118876737265924E-4 0.0 0.012531419457378349 0.0 40-41 0.0 1.118876737265924E-4 0.0 0.017286645590758525 0.0 42-43 0.0 1.118876737265924E-4 0.0 0.02120271417118926 0.0 44-45 0.0 1.118876737265924E-4 0.0 0.023524383401016053 0.0 46-47 0.0 1.118876737265924E-4 0.0 0.02634954716261251 0.0 48-49 0.0 1.118876737265924E-4 0.0 0.03034953149833819 0.0 50-51 0.0 1.118876737265924E-4 0.0 0.03499286995799177 0.0 52-53 0.0 1.118876737265924E-4 0.0 0.04567814279888135 0.0 54-55 0.0 1.118876737265924E-4 0.0 0.05384594298092259 0.0 56-57 0.0 1.118876737265924E-4 0.0 0.07706263527919052 0.0 58-59 0.0 1.118876737265924E-4 0.0 0.10970586408892385 0.0 60-61 0.0 1.118876737265924E-4 0.0 0.14176168261159255 0.0 62-63 0.0 1.118876737265924E-4 0.0 0.15692246240154584 5.59438368632962E-5 64-65 5.59438368632962E-5 1.118876737265924E-4 0.0 0.18467060548574077 5.59438368632962E-5 66-67 5.59438368632962E-5 1.678315105898886E-4 0.0 0.23571935662349852 5.59438368632962E-5 68-69 5.59438368632962E-5 1.678315105898886E-4 0.0 0.3092295582618697 5.59438368632962E-5 70-71 5.59438368632962E-5 1.678315105898886E-4 0.0 0.38290759141083086 5.59438368632962E-5 72-73 5.59438368632962E-5 1.678315105898886E-4 0.0 0.4651170596814446 5.59438368632962E-5 74-75 5.59438368632962E-5 1.678315105898886E-4 0.0 0.5615362625153356 5.59438368632962E-5 76-77 5.59438368632962E-5 1.678315105898886E-4 0.0 0.6283891475669745 5.59438368632962E-5 78-79 5.59438368632962E-5 1.678315105898886E-4 0.0 0.7254796764432251 5.59438368632962E-5 80-81 5.59438368632962E-5 1.678315105898886E-4 0.0 0.8508498148538719 5.59438368632962E-5 82-83 5.59438368632962E-5 1.678315105898886E-4 0.0 0.9736745086872387 5.59438368632962E-5 84-85 5.59438368632962E-5 1.678315105898886E-4 0.0 1.1153802474619678 5.59438368632962E-5 86-87 5.59438368632962E-5 1.678315105898886E-4 0.0 1.2812257518432095 5.59438368632962E-5 88-89 5.59438368632962E-5 1.678315105898886E-4 0.0 1.5162178385874854 5.59438368632962E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6760 0.0 36.410774 1 GTATCAA 10290 0.0 34.58703 1 ATCAACG 12775 0.0 27.666973 3 TCAACGC 12840 0.0 27.526915 4 TATCAAC 12945 0.0 27.340336 2 CAACGCA 12950 0.0 27.293095 5 AACGCAG 13245 0.0 26.71659 6 GTAGGAC 395 0.0 24.053736 3 TACATGG 8140 0.0 23.869785 2 GTACATG 8155 0.0 23.71468 1 CGCAGAG 15080 0.0 23.277267 8 ACGCAGA 15140 0.0 23.247765 7 GAGTACT 8700 0.0 22.82127 12-13 ACATGGG 8175 0.0 22.663473 3 GTACTTT 9200 0.0 21.528452 14-15 GCAGAGT 16230 0.0 21.423058 9 AGAGTAC 12965 0.0 21.230968 10-11 AGTACTT 9175 0.0 20.837355 12-13 GCGTACA 140 4.8489662E-4 20.35977 2 GTGTAGG 845 0.0 20.24383 1 >>END_MODULE