FastQCFastQC Report
Thu 26 May 2016
SRR937725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703366
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83820.4920844962268825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68040.39944439421709715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46250.27152121153058123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25800.1514648055673296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59750.039.5510981
GTATCAA92300.034.3608741
TCAACGC112850.027.6288834
ATCAACG112950.027.6060493
TATCAAC113350.027.469122
CAACGCA113900.027.416725
GTACATG81850.026.8969081
AACGCAG118050.026.4806866
TACATGG81850.025.7863122
ACATGGG82100.024.6634043
GAGTACT69150.023.42228712-13
CGCAGAG132100.023.4124838
ACGCAGA133650.023.2475997
AGGACCG4750.022.01425
GTACTTT73950.021.96491414-15
GCAGAGT139050.021.7968359
CATGGGG39800.021.2568054
AGAGTAC111850.020.93616910-11
AGTACTT74750.020.77799612-13
CATGGGA41100.020.1218764
Produced by FastQC (version 0.11.5)