FastQCFastQC Report
Thu 26 May 2016
SRR937725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703366
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97910.574803066399118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84370.4953133971207597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56780.33333998682608434No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.12170021005467996No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20430.11993899138529243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64050.038.734611
GTATCAA97500.034.3175241
ATCAACG120900.027.2309913
TCAACGC121450.027.1467914
TATCAAC121750.027.118922
CAACGCA122600.026.8534015
AACGCAG126450.026.0312166
TGTACCG2301.3460522E-1024.7862015
GTACCTG12750.023.4843331
GTACATG81650.023.0508461
TACCTGG12650.022.9084592
TATAACG1252.2543098E-422.8033052
GAGTACT81800.022.55955512-13
TACATGG81200.022.466312
ACGCAGA146800.022.2291817
CGCAGAG147150.022.014918
ACATGGG80300.022.0081713
GTACTTT86850.021.16514414-15
AGAGTAC125500.020.58960310-11
ATAGCGA2108.6152795E-720.3565086
Produced by FastQC (version 0.11.5)