##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937725_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1703366 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29778039481826 33.0 31.0 34.0 31.0 34.0 2 32.51523336734442 34.0 31.0 34.0 31.0 34.0 3 32.57512360819695 34.0 31.0 34.0 31.0 34.0 4 36.16376222138988 37.0 35.0 37.0 35.0 37.0 5 36.0933757043407 37.0 35.0 37.0 35.0 37.0 6 36.139537245665345 37.0 35.0 37.0 35.0 37.0 7 36.09857364770695 37.0 35.0 37.0 35.0 37.0 8 36.0595579575969 37.0 35.0 37.0 35.0 37.0 9 37.766975506144895 39.0 37.0 39.0 35.0 39.0 10-11 37.68912846681218 39.0 37.0 39.0 35.0 39.0 12-13 37.58643033851797 39.0 37.0 39.0 35.0 39.0 14-15 38.81539346212147 40.0 38.0 41.0 35.0 41.0 16-17 38.7751851334358 40.0 38.0 41.0 35.0 41.0 18-19 38.7582501353203 40.0 38.0 41.0 35.0 41.0 20-21 38.660213072234626 40.0 38.0 41.0 34.0 41.0 22-23 38.609396923503226 40.0 38.0 41.0 34.0 41.0 24-25 38.62921210121606 40.0 38.0 41.0 34.0 41.0 26-27 38.45799963131823 40.0 38.0 41.0 34.0 41.0 28-29 38.42987942696989 40.0 38.0 41.0 34.0 41.0 30-31 38.43657763510602 40.0 38.0 41.0 34.0 41.0 32-33 38.3069918619956 40.0 38.0 41.0 34.0 41.0 34-35 38.16403432967431 40.0 38.0 41.0 34.0 41.0 36-37 37.997638792837236 40.0 37.0 41.0 33.5 41.0 38-39 37.866606472126364 40.0 37.0 41.0 33.0 41.0 40-41 37.697528012182936 40.0 37.0 41.0 33.0 41.0 42-43 37.46394374432741 40.0 37.0 41.0 33.0 41.0 44-45 37.14868178653325 40.0 36.5 41.0 32.0 41.0 46-47 36.779919582755554 40.0 36.0 41.0 31.0 41.0 48-49 36.412871338279615 39.0 36.0 41.0 30.0 41.0 50-51 36.153290308718155 39.0 35.5 41.0 30.0 41.0 52-53 35.895025790112044 39.0 35.0 40.0 29.0 41.0 54-55 35.66303982819899 39.0 35.0 40.0 28.0 41.0 56-57 35.39178632190615 38.5 35.0 40.0 28.0 41.0 58-59 35.14127909092937 38.0 34.5 40.0 27.5 41.0 60-61 34.788562469839135 38.0 34.0 40.0 26.5 41.0 62-63 34.3779983280164 37.5 34.0 40.0 26.0 41.0 64-65 33.97990625620096 37.0 33.5 39.5 26.0 41.0 66-67 33.55607191877729 36.0 33.0 39.0 25.0 40.5 68-69 33.078270905959144 36.0 32.5 39.0 23.5 40.0 70-71 32.65096608714745 35.0 32.0 38.0 22.5 40.0 72-73 32.17110620970478 35.0 31.5 37.5 21.5 39.5 74-75 31.687282709646666 35.0 31.0 37.0 20.0 39.0 76-77 30.438527891245922 33.5 29.5 35.5 20.0 38.0 78-79 31.040663603711707 34.0 31.0 36.0 20.0 38.0 80-81 30.876116172331724 34.0 31.0 35.5 18.5 37.0 82-83 30.49921978012946 34.0 31.0 35.0 17.5 37.0 84-85 30.096808906600224 34.0 30.0 35.0 13.0 36.5 86-87 29.76701131759117 34.0 30.0 35.0 7.5 36.0 88-89 29.3597465254091 34.0 29.5 35.0 4.5 36.0 90-91 28.86680138032578 34.0 29.0 35.0 2.0 35.0 92-93 28.409395279699137 34.0 29.0 35.0 2.0 35.0 94-95 27.906154343811018 33.0 27.5 35.0 2.0 35.0 96-97 27.38320155503867 33.0 27.0 35.0 2.0 35.0 98-99 26.560143269268025 33.0 25.5 34.5 2.0 35.0 100-101 24.104163168690697 30.5 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18626296636398365 1101 2 0.16415721521033078 1101 3 0.15665248660096154 1101 4 0.09544715847218299 1101 5 0.048636810254450324 1101 6 0.03925233548252294 1101 7 0.09745688078680104 1101 8 0.11830220633437705 1101 9 0.122816339459483 1101 10-11 0.09649187878047627 1101 12-13 0.15394626998679684 1101 14-15 0.24099120928583773 1101 16-17 0.2712021167341234 1101 18-19 0.2934691722790248 1101 20-21 0.25452731680183405 1101 22-23 0.22739932074646418 1101 24-25 0.19980248386863764 1101 26-27 0.08322218770980783 1101 28-29 0.048748688534921314 1101 30-31 0.19594150092537888 1101 32-33 0.21426081806077946 1101 34-35 0.13527305897323316 1101 36-37 0.13146866012936442 1101 38-39 0.21214271778778482 1101 40-41 0.16065200454040962 1101 42-43 0.2853064615239447 1101 44-45 0.23062617875977054 1101 46-47 0.24607052294542342 1101 48-49 0.37427785491293974 1101 50-51 0.34435328925643205 1101 52-53 0.3282648653725815 1101 54-55 0.30970649496239844 1101 56-57 0.461171755558361 1101 58-59 0.39730328176278107 1101 60-61 0.4375878099867734 1101 62-63 0.3750601994050271 1101 64-65 0.49459261338210325 1101 66-67 0.369779242168903 1101 68-69 0.6263712185180523 1101 70-71 0.4966702897310782 1101 72-73 0.6100298349938456 1101 74-75 0.5007165110713565 1101 76-77 0.48125510233559865 1101 78-79 0.6844459095530055 1101 80-81 0.55420590875746 1101 82-83 0.6433879992759621 1101 84-85 0.6654813494032723 1101 86-87 0.6921297954390617 1101 88-89 0.8711392195205114 1101 90-91 0.7050925853367822 1101 92-93 0.7836127269130593 1101 94-95 0.6363825428124059 1101 96-97 0.6676677554088783 1101 98-99 0.8175342261560203 1101 100-101 1.36587681016929 1102 1 0.22449549036404903 1102 2 0.14708004612290182 1102 3 0.1898106838855398 1102 4 0.14018081249297865 1102 5 0.05721916320664633 1102 6 0.10532765272157008 1102 7 0.09824705269600287 1102 8 0.0987960076042711 1102 9 0.22061836956466152 1102 10-11 0.1694865835052255 1102 12-13 0.08361160784146904 1102 14-15 0.25884427288718825 1102 16-17 0.1660062683197978 1102 18-19 0.2153413928680621 1102 20-21 0.13760083274851098 1102 22-23 0.14196760039991574 1102 24-25 0.10809456099092074 1102 26-27 0.11074455874981481 1102 28-29 0.14862772856967155 1102 30-31 0.06841627440631015 1102 32-33 0.1441534239616047 1102 34-35 0.006137061399925869 1102 36-37 0.0977632407109823 1102 38-39 0.09048713911793271 1102 40-41 0.13834018061018583 1102 42-43 0.14318107697049243 1102 44-45 0.1611455857189057 1102 46-47 0.04782561271981223 1102 48-49 0.15959030049500456 1102 50-51 0.1488000191243657 1102 52-53 0.17891090580616975 1102 54-55 0.11682379991444947 1102 56-57 0.28653674195503953 1102 58-59 0.22877641872654664 1102 60-61 0.20722063282910597 1102 62-63 0.15047092027773346 1102 64-65 0.22070184996744047 1102 66-67 0.2523633477298759 1102 68-69 0.1341736980100947 1102 70-71 0.051446335913063024 1102 72-73 0.2894215617816087 1102 74-75 0.3636954123579166 1102 76-77 0.14867183937500172 1102 78-79 0.12162771088281588 1102 80-81 0.07965946181600714 1102 82-83 0.2747709311882325 1102 84-85 0.20632055129219395 1102 86-87 0.1305071530817301 1102 88-89 0.20624677277753634 1102 90-91 0.4596014335769638 1102 92-93 0.6680753632036733 1102 94-95 0.7044983572980179 1102 96-97 0.5776621193485774 1102 98-99 0.5683492079652126 1102 100-101 0.9674122621120453 1103 1 0.16566614805111612 1103 2 0.15818035944103315 1103 3 0.15409288563618873 1103 4 0.07425849103513826 1103 5 0.053056106645463785 1103 6 0.052413653630139834 1103 7 0.12902247993735472 1103 8 0.03557552811982134 1103 9 0.07771837377970314 1103 10-11 0.05618847699579277 1103 12-13 0.13516953422809763 1103 14-15 0.13229383990869792 1103 16-17 0.12811374252608942 1103 18-19 0.20946632671530807 1103 20-21 0.22300285409536258 1103 22-23 0.21739273422750216 1103 24-25 -0.02715829470008657 1103 26-27 0.01447732976531313 1103 28-29 -0.010827547492162637 1103 30-31 0.019966239046787848 1103 32-33 0.10675042847169891 1103 34-35 0.09569757301235882 1103 36-37 0.09737733019164097 1103 38-39 0.13855524483118842 1103 40-41 -0.016916796455305416 1103 42-43 0.05586706570228728 1103 44-45 0.16493268394342664 1103 46-47 0.18165659421577374 1103 48-49 0.21797612671350208 1103 50-51 0.19445114945814623 1103 52-53 0.07750554052593372 1103 54-55 0.07553734565468773 1103 56-57 0.09619480837474015 1103 58-59 0.10650753182548556 1103 60-61 0.15100168949332726 1103 62-63 0.06475768832397222 1103 64-65 0.031131620341312782 1103 66-67 0.06077586921488631 1103 68-69 0.0637476040895919 1103 70-71 0.11149429372517972 1103 72-73 0.12371455872336412 1103 74-75 0.0719900297491769 1103 76-77 0.11437427613766005 1103 78-79 0.28623962259173297 1103 80-81 0.2377076163300842 1103 82-83 0.26186259898671693 1103 84-85 0.24793765218625197 1103 86-87 0.3209277674475288 1103 88-89 0.4039473502383011 1103 90-91 0.3443603097530783 1103 92-93 0.5260900315501118 1103 94-95 0.43762624635811065 1103 96-97 0.46818389472430155 1103 98-99 0.6157944241266264 1103 100-101 1.08743242406333 1104 1 0.1268464844018169 1104 2 0.11414319793051675 1104 3 0.1362303532707756 1104 4 0.10449714980778424 1104 5 0.10039051258549136 1104 6 0.0438857495928886 1104 7 0.05835592824686131 1104 8 0.14430040450152148 1104 9 0.0322148121657051 1104 10-11 0.1770465547907918 1104 12-13 0.1284679359836005 1104 14-15 0.1518555991349686 1104 16-17 0.1812159434877998 1104 18-19 0.21201630163991325 1104 20-21 0.1067602377216943 1104 22-23 0.18130038869080778 1104 24-25 0.1352910155563194 1104 26-27 0.13788617672823733 1104 28-29 0.21355424754313646 1104 30-31 0.21091163822213588 1104 32-33 0.19978514144351323 1104 34-35 0.1829252326495947 1104 36-37 0.18962449417565352 1104 38-39 0.2286155165531767 1104 40-41 0.2064397439115453 1104 42-43 0.35671225406275653 1104 44-45 0.27007348059218117 1104 46-47 0.2116299757180755 1104 48-49 0.18280113221647554 1104 50-51 0.19475557157805667 1104 52-53 0.3911160345225895 1104 54-55 0.3875096560631306 1104 56-57 0.2992987112864327 1104 58-59 0.3682682331196503 1104 60-61 0.26159110881068415 1104 62-63 0.2931382715708821 1104 64-65 0.3351233738263417 1104 66-67 0.41371252717512874 1104 68-69 0.4886523516223278 1104 70-71 0.4706221918623399 1104 72-73 0.5342993260441062 1104 74-75 0.5259144994782936 1104 76-77 0.51331021700965 1104 78-79 0.5668858416531215 1104 80-81 0.6114646822590863 1104 82-83 0.5779225393957752 1104 84-85 0.566365118481972 1104 86-87 0.7058077572812138 1104 88-89 0.6785817146507505 1104 90-91 0.5214122373816252 1104 92-93 0.6270227001396016 1104 94-95 0.748781393849093 1104 96-97 0.8975289162936839 1104 98-99 0.8202535330763823 1104 100-101 1.002784353280365 1105 1 0.09070473462492146 1105 2 0.09052772542503362 1105 3 0.1489017856294339 1105 4 0.13241593390289097 1105 5 0.12498099787955397 1105 6 0.1061502809706596 1105 7 0.09626754326756526 1105 8 0.07538674330749728 1105 9 0.17043419331029241 1105 10-11 0.10414206217082977 1105 12-13 0.04120242445894462 1105 14-15 0.11017251889735036 1105 16-17 0.23973071290124182 1105 18-19 0.27129745257506954 1105 20-21 0.2357599371433139 1105 22-23 0.15942677723223397 1105 24-25 0.11434807690790905 1105 26-27 0.13697137296298223 1105 28-29 0.12098684144120142 1105 30-31 0.16304453120503126 1105 32-33 0.09074519999094832 1105 34-35 0.25805395860799507 1105 36-37 0.22105103730024922 1105 38-39 0.18396959228399368 1105 40-41 0.12209857064771512 1105 42-43 0.25245963006950944 1105 44-45 0.25995393067562844 1105 46-47 0.1754249831298509 1105 48-49 0.24267527202089667 1105 50-51 0.2316644029815862 1105 52-53 0.2924360123432663 1105 54-55 0.34434842096603546 1105 56-57 0.35395613594581476 1105 58-59 0.3965029979030632 1105 60-61 0.4702473460921084 1105 62-63 0.3287794463282765 1105 64-65 0.32416276452969583 1105 66-67 0.41343761930894374 1105 68-69 0.2225214674738183 1105 70-71 0.2317155877259296 1105 72-73 0.2865833016692534 1105 74-75 0.29525303101288713 1105 76-77 0.17681458753131096 1105 78-79 0.28047828537402 1105 80-81 0.4013172214929668 1105 82-83 0.4348236603104283 1105 84-85 0.4679172977685724 1105 86-87 0.5901044505456419 1105 88-89 0.7015325542165733 1105 90-91 0.8906480401872727 1105 92-93 0.8207176952295328 1105 94-95 0.8572283464323895 1105 96-97 0.8946687543174079 1105 98-99 1.02999633319736 1105 100-101 1.1139922863092586 1106 1 -0.17469740946396684 1106 2 -0.0863861542375517 1106 3 0.014723148366122984 1106 4 -0.06040234521942267 1106 5 0.03686225748930383 1106 6 0.03916874018285199 1106 7 0.006179069869048703 1106 8 0.00432981961633061 1106 9 -0.1784559603607363 1106 10-11 -0.2110985744298901 1106 12-13 -0.20029268253313148 1106 14-15 -0.10677684781490626 1106 16-17 -0.06233925881105762 1106 18-19 -0.05062275571756203 1106 20-21 -0.04431220499176902 1106 22-23 -0.07968182036033511 1106 24-25 -0.04596589110064997 1106 26-27 0.01683358721896866 1106 28-29 -0.08917039740723709 1106 30-31 -0.02041990529205151 1106 32-33 0.003599114822030458 1106 34-35 -0.21117785613729012 1106 36-37 -0.14957254512909657 1106 38-39 -0.001100893364032629 1106 40-41 -0.053963704770865206 1106 42-43 -0.1806034268711727 1106 44-45 0.07106184443636465 1106 46-47 0.09052418122022488 1106 48-49 -1.8006569009543227E-4 1106 50-51 -0.034167259706677555 1106 52-53 0.04925574832471824 1106 54-55 0.03717678766979304 1106 56-57 -0.033607150354619364 1106 58-59 0.13706208352220983 1106 60-61 0.22246820678579837 1106 62-63 0.196387372831218 1106 64-65 -0.22018383627391103 1106 66-67 -0.0742364180172359 1106 68-69 0.013164864036824042 1106 70-71 -0.025079194764579427 1106 72-73 0.054700067017243725 1106 74-75 0.05997293604096399 1106 76-77 0.1285407319939722 1106 78-79 0.2680084419332829 1106 80-81 0.23805183952404008 1106 82-83 -0.13166040343459073 1106 84-85 0.15307750979891566 1106 86-87 0.3309274060583469 1106 88-89 0.30998254954443283 1106 90-91 0.23584507719914072 1106 92-93 0.3345031716606961 1106 94-95 0.5761304565420602 1106 96-97 0.22230915221409475 1106 98-99 -0.05432682194327043 1106 100-101 0.5057196200446832 1107 1 0.0769017376575647 1107 2 0.07764590222888756 1107 3 0.04541942069904081 1107 4 -1.8618464299891002E-4 1107 5 0.01182733358665189 1107 6 0.009354755916987756 1107 7 9.58865472313164E-4 1107 8 0.007564875862193787 1107 9 0.01594151041347658 1107 10-11 -0.010230991163950875 1107 12-13 -0.03864096574760367 1107 14-15 0.015521144979736334 1107 16-17 0.01220489235429767 1107 18-19 0.08127011980629817 1107 20-21 0.02006725240106988 1107 22-23 -0.0580473817161149 1107 24-25 -0.039468207106828856 1107 26-27 -0.16245470040694698 1107 28-29 -0.06888696236570269 1107 30-31 -0.006753630753415507 1107 32-33 -0.048014737100629645 1107 34-35 -7.205509324137438E-5 1107 36-37 -0.08882333551215282 1107 38-39 -0.03379150434852107 1107 40-41 0.01006651359543298 1107 42-43 -0.01755475397946782 1107 44-45 0.03900537800007697 1107 46-47 -0.2378799802384961 1107 48-49 -0.12796478740173 1107 50-51 -0.17875221669513763 1107 52-53 -0.024856449307812056 1107 54-55 0.14460455033430009 1107 56-57 -0.058550169250288775 1107 58-59 0.03720729343324791 1107 60-61 0.1877464951469463 1107 62-63 0.05556133062843571 1107 64-65 0.1406168401469401 1107 66-67 0.31892401136295945 1107 68-69 0.3641041332082082 1107 70-71 0.315107188589586 1107 72-73 0.12024671799878917 1107 74-75 0.123782582390497 1107 76-77 0.019394345698845683 1107 78-79 0.16184896836516316 1107 80-81 0.26214014185475065 1107 82-83 0.25736430063128424 1107 84-85 0.20870761052680464 1107 86-87 0.45669021761631967 1107 88-89 0.5533837700429238 1107 90-91 0.48883198992614396 1107 92-93 0.3711181264434309 1107 94-95 0.5233586015313989 1107 96-97 0.5436825806384746 1107 98-99 0.6786247992610548 1107 100-101 1.1291388539245446 1108 1 0.01638670276911114 1108 2 -0.04919117674406692 1108 3 -0.020035672086869738 1108 4 0.0591649128299494 1108 5 -0.042619042211647695 1108 6 -0.0036495135619034613 1108 7 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56-57 -0.44987811804028865 2316 58-59 -0.39014794434022804 2316 60-61 -0.34745169414775035 2316 62-63 -0.31037711760006204 2316 64-65 -0.3464935671986069 2316 66-67 -0.4104437468419988 2316 68-69 -0.460772962169564 2316 70-71 -0.21970368406216778 2316 72-73 -0.09064245786927927 2316 74-75 -0.22664557089848714 2316 76-77 0.1545783789912356 2316 78-79 0.02183952752199403 2316 80-81 -0.10943781561830335 2316 82-83 -0.021486579303708453 2316 84-85 0.125322442017751 2316 86-87 0.18290655501121478 2316 88-89 0.1330854836033204 2316 90-91 0.025728373251741488 2316 92-93 0.14313202563796068 2316 94-95 0.20514903586281008 2316 96-97 0.21840448623635567 2316 98-99 0.44141151093223385 2316 100-101 0.6265402539479084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 13.0 5 32.0 6 53.0 7 129.0 8 371.0 9 734.0 10 1140.0 11 2174.0 12 3211.0 13 4885.0 14 6338.0 15 8733.0 16 12629.0 17 18327.0 18 16930.0 19 10048.0 20 8446.0 21 8444.0 22 8688.0 23 9339.0 24 10683.0 25 12581.0 26 15309.0 27 18972.0 28 24178.0 29 30101.0 30 37616.0 31 48174.0 32 62314.0 33 82579.0 34 114903.0 35 166419.0 36 257326.0 37 387042.0 38 290464.0 39 24032.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.30800131554219 19.61567374553655 15.193925014095095 21.88239992482616 2 15.85184863382268 20.606023602678462 40.170462484281124 23.371665279217737 3 19.711030982184685 24.582914065444537 30.056370738878197 25.64968421349258 4 13.187727344243507 16.84073398287154 36.35987375569755 33.6116649171874 5 13.494861697674187 37.95838277490049 33.92254177310915 14.624213754316168 6 32.33016274834651 36.144903678950975 16.893785598632356 14.631147974070164 7 28.814946406115894 33.360769206383125 19.844002991723446 17.980281395777535 8 26.695026201063072 35.619708271739604 19.019282996138234 18.665982531059093 9 26.71533892305001 16.854216885860115 20.253134088622176 36.1773101024677 10-11 25.361548839180774 27.143226998777713 26.332244508813723 21.162979653227783 12-13 26.1317161151537 24.364833430598136 27.59453375714955 21.90891669709861 14-15 22.99359033818921 25.525166053566878 26.31096311655863 25.170280491685286 16-17 21.950831471333817 29.130321962514223 26.884562683533662 22.034283882618297 18-19 21.795785521138733 27.2142921720875 29.90681392020271 21.08310838657106 20-21 22.51952315479622 26.053093822687135 30.39183684971251 21.035546172804136 22-23 22.375036142173403 25.903921260697715 30.30600128864277 21.41504130848611 24-25 22.271343915517864 26.09788501120722 30.394377955178157 21.236393118096757 26-27 21.867329440213883 26.514503503983626 30.330020835449268 21.28814622035322 28-29 21.70197439315319 26.73384278533828 30.286728934762337 21.277453886746194 30-31 21.84022693889628 26.369875881049637 30.32169833142143 21.46819884863265 32-33 21.794523314425675 26.38531589805127 30.24872517121981 21.57143561630325 34-35 22.26611896679868 26.446107295789627 30.1227980363586 21.16497570105309 36-37 22.214825069069448 26.27521636672972 30.0494376402377 21.460520923963138 38-39 21.927612738542393 26.660682437010014 30.086516931769214 21.32518789267838 40-41 22.188817706154737 26.469527185199865 29.96787237009151 21.37378273855389 42-43 21.918141635141463 26.571393758963946 29.731694463161386 21.778770142733205 44-45 22.105613238728495 26.72021162803531 29.590117449802335 21.584057683433862 46-47 22.29294819786235 27.065909499191598 29.02271150181464 21.618430801131407 48-49 22.419638527480295 27.112787269441803 28.771503012270998 21.696071190806908 50-51 22.378514071550097 27.339984477792793 28.632983163923665 21.648518286733445 52-53 22.602247549851295 27.476126680936453 28.246307605059627 21.675318164152625 54-55 22.355735643426016 27.437761467588295 28.311296573960032 21.89520631502566 56-57 22.389932639256624 27.723137599318054 28.23682637788942 21.650103383535892 58-59 22.582668668976602 27.252745446369133 28.38236174726982 21.78222413738445 60-61 22.495048040174574 27.305846189251167 28.710564846310188 21.488540924264075 62-63 22.31648982074316 27.326775337772386 28.848967280079563 21.50776756140489 64-65 22.269670757781945 27.33355602964953 28.565058830574287 21.83171438199424 66-67 22.315755979630918 27.575899718557256 28.241200070918403 21.86714423089342 68-69 22.29749801275827 27.44225257519523 28.49199760943919 21.76825180260731 70-71 22.396889453000707 27.4483874868936 28.30023024998738 21.85449281011832 72-73 22.430734205097437 27.41401436919605 28.223264994135143 21.93198643157137 74-75 22.220209866816646 27.862772886156 27.92755637954497 21.989460867482386 76-77 22.384913166048868 27.546164476689093 28.09032233824087 21.97860001902116 78-79 22.467719797154576 27.569588684991952 28.01279936314333 21.94989215471014 80-81 22.375989658123974 27.902899318173546 27.900345551102934 21.820765472599547 82-83 22.466353365250548 27.810803908733593 27.876379989021732 21.846462736994127 84-85 22.328045465312915 27.798806421056675 27.75976603950593 22.113382074124473 86-87 22.360872531211733 27.796580417831517 27.910296436584975 21.932250614371778 88-89 22.375725475323566 27.66331487184786 27.821208125558456 22.139751527270125 90-91 22.429988850353425 27.607587630996605 27.86357741019368 22.098846108456293 92-93 22.440387148892402 27.565395661659203 27.877432596499553 22.116784592948846 94-95 22.342996161717448 27.55875719017522 27.82696143987845 22.271285208228882 96-97 22.303662278101125 27.544726148109095 27.853555841786203 22.298055732003576 98-99 22.173755809120347 27.61061105253394 27.583752310145382 22.63188082820033 100-101 22.167865425828612 27.606640636467723 27.937791738836857 22.287702198866803 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 445.0 1 327.5 2 295.5 3 386.5 4 326.5 5 308.5 6 624.0 7 1459.0 8 2415.0 9 2882.5 10 2715.5 11 2466.0 12 2561.5 13 2812.5 14 3054.0 15 3240.0 16 3338.0 17 3397.5 18 3585.0 19 3776.0 20 4018.5 21 4489.5 22 5122.0 23 5990.0 24 7081.5 25 8545.0 26 10649.5 27 12955.5 28 14768.5 29 16934.5 30 19720.0 31 22356.5 32 25528.5 33 28953.0 34 32662.5 35 35780.5 36 39795.0 37 44401.0 38 47390.5 39 49325.5 40 49904.0 41 52176.0 42 54916.5 43 57365.0 44 60372.5 45 62175.0 46 62817.5 47 65490.0 48 69864.0 49 68964.0 50 65206.5 51 62721.0 52 60200.0 53 58907.5 54 57909.5 55 53092.0 56 46338.5 57 40415.0 58 35928.0 59 31705.5 60 26098.5 61 20972.5 62 16782.5 63 12720.5 64 9275.0 65 6861.5 66 4904.0 67 3806.0 68 2835.0 69 1894.0 70 1332.0 71 841.5 72 598.5 73 449.5 74 312.0 75 218.5 76 144.0 77 73.0 78 45.0 79 33.0 80 16.5 81 9.0 82 5.5 83 3.0 84 1.0 85 2.0 86 1.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03792490868081199 2 0.0 3 0.0 4 1.1741457795917025E-4 5 0.010391190149386567 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006575216365713534 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.348291559183405E-4 22-23 0.0013796212910202504 24-25 0.0 26-27 0.0011154384906121174 28-29 0.0019079868918365165 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.69658311836681E-4 38-39 0.0 40-41 2.0547551142854794E-4 42-43 0.021340099544079193 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.8707288979585126E-5 84-85 8.806093346937769E-5 86-87 0.0 88-89 0.0 90-91 0.010156360993468228 92-93 0.0024657061371425753 94-95 0.0 96-97 0.0 98-99 5.870728897958513E-4 100-101 0.012680774419590387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1703366.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.35028698090514 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8506988545724 42.537082362837 2 13.232426736681749 14.648372346582736 3 4.185684095817885 6.950364477447911 4 1.9063152182352185 4.220603776215446 5 0.9666143505719994 2.67511908520107 6 0.5933118462885021 1.970398857674356 7 0.39048076017100863 1.5129255495191123 8 0.28642044249564946 1.2682762951465627 9 0.2075088061518242 1.0337104774411632 >10 1.2349635432151682 12.727251225052111 >50 0.09766149414034322 3.6888020335440648 >100 0.04568798251949182 4.493244787062772 >500 0.0015899065277647562 0.5389229165480517 >1k 3.1798130555295127E-4 0.3324120667495385 >5k 3.1798130555295127E-4 1.4025137429781978 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9791 0.574803066399118 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8437 0.4953133971207597 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5678 0.33333998682608434 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2073 0.12170021005467996 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2043 0.11993899138529243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.8707288979585126E-5 0.0 14-15 0.0 0.0 0.0 5.8707288979585126E-5 0.0 16-17 0.0 0.0 0.0 1.7612186693875538E-4 0.0 18-19 0.0 0.0 0.0 1.7612186693875538E-4 0.0 20-21 0.0 0.0 0.0 2.0547551142854794E-4 0.0 22-23 0.0 0.0 5.8707288979585126E-5 2.6418280040813307E-4 0.0 24-25 0.0 0.0 8.806093346937769E-5 3.5224373387751076E-4 0.0 26-27 0.0 0.0 1.1741457795917025E-4 7.338411122448141E-4 0.0 28-29 2.9353644489792563E-5 0.0 1.1741457795917025E-4 0.0014970358689794208 0.0 30-31 5.8707288979585126E-5 5.8707288979585126E-5 1.1741457795917025E-4 0.002817949871020086 0.0 32-33 5.8707288979585126E-5 1.1741457795917025E-4 1.1741457795917025E-4 0.0047846440518361875 0.0 34-35 5.8707288979585126E-5 1.1741457795917025E-4 1.1741457795917025E-4 0.005782667964489135 0.0 36-37 5.8707288979585126E-5 1.1741457795917025E-4 1.1741457795917025E-4 0.007573240278366481 0.0 38-39 5.8707288979585126E-5 1.1741457795917025E-4 1.1741457795917025E-4 0.011624043217957854 0.0 40-41 5.8707288979585126E-5 1.4676822244896282E-4 1.1741457795917025E-4 0.016232565402855288 0.0 42-43 5.8707288979585126E-5 2.0547551142854794E-4 1.1741457795917025E-4 0.020547551142854795 0.0 44-45 5.8707288979585126E-5 2.348291559183405E-4 1.1741457795917025E-4 0.023101318213466747 0.0 46-47 5.8707288979585126E-5 2.348291559183405E-4 1.1741457795917025E-4 0.027269535731017293 0.0 48-49 1.1741457795917025E-4 2.348291559183405E-4 1.1741457795917025E-4 0.03202482613836369 0.0 50-51 1.1741457795917025E-4 2.348291559183405E-4 1.1741457795917025E-4 0.0371030066350978 0.0 52-53 1.1741457795917025E-4 2.6418280040813307E-4 1.1741457795917025E-4 0.04995990292162694 0.0 54-55 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.05917694729142181 0.0 56-57 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.08019415674611328 0.0 58-59 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.11380407968692577 0.0 60-61 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.1448308819126365 0.0 62-63 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.16071120358161428 0.0 64-65 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.18809815389059076 0.0 66-67 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.24466262682242101 0.0 68-69 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.3219507727640448 0.0 70-71 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.39342689709668976 0.0 72-73 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.4733568710423949 0.0 74-75 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.573247323241159 0.0 76-77 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.6440483137505386 0.0 78-79 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.7479308615999145 0.0 80-81 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 0.8833098699868378 0.0 82-83 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 1.0068887132888644 0.0 84-85 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 1.152453436313746 0.0 86-87 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 1.3255812315145423 0.0 88-89 1.1741457795917025E-4 2.9353644489792563E-4 1.1741457795917025E-4 1.5679836277112493 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6405 0.0 38.73461 1 GTATCAA 9750 0.0 34.317524 1 ATCAACG 12090 0.0 27.230991 3 TCAACGC 12145 0.0 27.146791 4 TATCAAC 12175 0.0 27.11892 2 CAACGCA 12260 0.0 26.853401 5 AACGCAG 12645 0.0 26.031216 6 TGTACCG 230 1.3460522E-10 24.786201 5 GTACCTG 1275 0.0 23.484333 1 GTACATG 8165 0.0 23.050846 1 TACCTGG 1265 0.0 22.908459 2 TATAACG 125 2.2543098E-4 22.803305 2 GAGTACT 8180 0.0 22.559555 12-13 TACATGG 8120 0.0 22.46631 2 ACGCAGA 14680 0.0 22.229181 7 CGCAGAG 14715 0.0 22.01491 8 ACATGGG 8030 0.0 22.008171 3 GTACTTT 8685 0.0 21.165144 14-15 AGAGTAC 12550 0.0 20.589603 10-11 ATAGCGA 210 8.6152795E-7 20.356508 6 >>END_MODULE