FastQCFastQC Report
Thu 26 May 2016
SRR937724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416926
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71610.505389836872215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58030.40954855793457107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38240.2698800078479751No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20840.14707895825187767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48700.038.582971
GTATCAA76000.037.679961
ATCAACG92450.030.542143
TCAACGC93050.030.3494954
TATCAAC94200.030.0252042
CAACGCA94650.029.7883385
AACGCAG96650.029.1512766
GTACATG66300.026.1882741
TACATGG66450.025.5383682
ACGCAGA110450.025.2070057
CGCAGAG110500.025.0666128
ACATGGG66400.023.982623
GAGTACT60100.023.78802912-13
CATGGGG33100.023.4121344
GCAGAGT116000.023.3849669
ACACCGT2606.2391337E-1021.9286866
GTACTTT65700.021.57817514-15
AGAGTAC95500.021.56169510-11
AGTACTT63300.021.347412-13
GTACCTG11150.020.4782981
Produced by FastQC (version 0.11.5)