##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937724_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1416926 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63567328145577 33.0 31.0 34.0 30.0 34.0 2 31.740380231571727 33.0 31.0 34.0 30.0 34.0 3 31.775688356343238 33.0 31.0 34.0 30.0 34.0 4 35.40098988938025 37.0 35.0 37.0 33.0 37.0 5 35.39708778016636 37.0 35.0 37.0 33.0 37.0 6 35.451870457596236 37.0 35.0 37.0 33.0 37.0 7 35.365305598175205 37.0 35.0 37.0 33.0 37.0 8 35.36405218056554 37.0 35.0 37.0 33.0 37.0 9 36.91521222703232 39.0 37.0 39.0 33.0 39.0 10-11 36.81816093430426 39.0 37.0 39.0 33.0 39.0 12-13 36.705671291231866 39.0 37.0 39.0 32.5 39.0 14-15 37.794559842927576 40.0 38.0 41.0 32.5 41.0 16-17 37.689863126232424 40.0 37.0 41.0 32.0 41.0 18-19 37.62175300615558 40.0 37.0 41.0 32.0 41.0 20-21 37.635327109531474 40.0 37.5 41.0 32.0 41.0 22-23 37.64286031874636 40.0 37.5 41.0 32.0 41.0 24-25 37.556699855885206 40.0 37.0 41.0 32.0 41.0 26-27 37.44062745690319 40.0 37.0 41.0 32.0 41.0 28-29 37.28512568758001 40.0 37.0 41.0 31.5 41.0 30-31 37.02707092678094 39.0 36.5 41.0 31.0 41.0 32-33 36.87744349387336 39.0 36.0 41.0 31.0 41.0 34-35 36.840869247935316 39.0 36.0 41.0 30.5 41.0 36-37 36.66342067263922 39.0 36.0 41.0 30.0 41.0 38-39 36.482498380296505 39.0 35.5 41.0 30.0 41.0 40-41 36.23407820874202 39.0 35.0 41.0 30.0 41.0 42-43 35.94307747899326 39.0 35.0 40.0 29.0 41.0 44-45 35.66580153091975 39.0 35.0 40.0 27.5 41.0 46-47 35.241250778092855 38.5 35.0 40.0 26.0 41.0 48-49 34.8315127254352 38.0 34.0 40.0 24.0 41.0 50-51 33.941156066018976 37.5 33.5 39.5 23.5 40.5 52-53 34.0528746737656 38.0 33.5 39.5 23.0 40.5 54-55 34.28721330542315 38.0 34.0 40.0 23.0 41.0 56-57 34.00924324911816 38.0 33.5 40.0 22.0 41.0 58-59 33.70000409336832 38.0 33.0 40.0 20.5 41.0 60-61 33.316033088531086 37.0 33.0 40.0 18.5 41.0 62-63 32.91458763548697 37.0 32.0 40.0 17.0 41.0 64-65 32.44739210092835 36.0 31.5 39.0 12.5 41.0 66-67 31.902469148000673 36.0 31.0 39.0 8.0 40.0 68-69 31.391787573945287 35.0 30.5 38.5 7.5 40.0 70-71 30.891472808036553 35.0 30.0 38.0 4.0 40.0 72-73 30.388623329658714 35.0 29.5 37.0 2.0 39.0 74-75 29.81028084741193 34.0 29.0 36.5 2.0 39.0 76-77 29.238217451017203 34.0 28.5 36.0 2.0 39.0 78-79 28.678602834586986 34.0 27.5 35.5 2.0 37.5 80-81 28.098341409501977 34.0 26.5 35.0 2.0 37.0 82-83 27.501700512235644 33.0 26.0 35.0 2.0 36.5 84-85 26.92016096818041 33.0 25.0 35.0 2.0 36.0 86-87 26.295260655814065 32.0 24.5 35.0 2.0 36.0 88-89 25.690870588866318 32.0 21.5 34.5 2.0 35.0 90-91 25.056417201745187 31.5 19.0 34.0 2.0 35.0 92-93 24.32849351342272 31.0 14.5 34.0 2.0 35.0 94-95 23.484073621346493 31.0 4.5 34.0 2.0 35.0 96-97 22.52304072336876 30.0 2.0 34.0 2.0 35.0 98-99 21.274215449501245 29.5 2.0 34.0 2.0 35.0 100-101 18.136267878491893 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11616397526286804 1101 2 -0.0137857683573408 1101 3 0.07855900802705307 1101 4 0.22253325084293607 1101 5 0.13217027264348502 1101 6 0.05920988035419583 1101 7 0.07649942065941673 1101 8 0.07498129621630056 1101 9 0.3610034162164908 1101 10-11 0.23448354991871412 1101 12-13 0.3160144447308113 1101 14-15 0.4652082919294003 1101 16-17 0.4195831258695151 1101 18-19 0.5379849848559317 1101 20-21 0.44281308166136313 1101 22-23 0.4089607451295265 1101 24-25 0.39557999142598277 1101 26-27 0.45216288526463444 1101 28-29 0.42200893832880837 1101 30-31 0.48494461832393654 1101 32-33 0.5152801499659958 1101 34-35 0.7043910747355397 1101 36-37 0.5974610574847006 1101 38-39 0.5834363017348423 1101 40-41 0.5100087750290925 1101 42-43 0.43193954329147743 1101 44-45 0.660551773154225 1101 46-47 0.6311386969476374 1101 48-49 0.8645022922884706 1101 50-51 0.6461554653617583 1101 52-53 0.7926479031034717 1101 54-55 0.8778823766023507 1101 56-57 1.0308540070041659 1101 58-59 0.907680513853478 1101 60-61 0.9911755477557378 1101 62-63 1.0269079907548715 1101 64-65 1.0792446803667914 1101 66-67 1.2100485562997676 1101 68-69 1.3073681238358645 1101 70-71 1.0896723227594443 1101 72-73 1.3346286751671776 1101 74-75 1.3571256040914896 1101 76-77 1.4070191267449488 1101 78-79 1.490165885109576 1101 80-81 1.8691188095649416 1101 82-83 1.9439484844376196 1101 84-85 2.2032518152199643 1101 86-87 2.4823111788739993 1101 88-89 2.4761291180330183 1101 90-91 2.503769735897908 1101 92-93 2.606350580090975 1101 94-95 2.8201938007296974 1101 96-97 2.763491810226668 1101 98-99 3.0691200994889236 1101 100-101 3.7833187107019413 1102 1 -0.1289171995375149 1102 2 -0.12301439468052422 1102 3 -0.10821998271217481 1102 4 -0.07981834671901566 1102 5 -0.26472640755434895 1102 6 -0.17507664100060794 1102 7 -0.09175413401030141 1102 8 -0.13053571676533693 1102 9 0.043385550211525015 1102 10-11 -0.018659827644555094 1102 12-13 -0.11580152333986149 1102 14-15 0.011253288118439286 1102 16-17 0.02424090596336015 1102 18-19 -0.04463566936401264 1102 20-21 -0.05888736420951801 1102 22-23 -0.12991805153045988 1102 24-25 -0.05120844758760512 1102 26-27 -4.190748626697882E-4 1102 28-29 -0.04825834068993373 1102 30-31 0.07391071411677075 1102 32-33 0.0258424927484171 1102 34-35 0.13684906278883346 1102 36-37 0.1690038535407723 1102 38-39 0.32208351826835013 1102 40-41 0.33082164318913954 1102 42-43 0.2433501609115396 1102 44-45 0.2573672420248414 1102 46-47 0.2628348570033552 1102 48-49 0.43355456705792506 1102 50-51 0.3001451696648587 1102 52-53 0.28430553952510706 1102 54-55 0.5468669549731047 1102 56-57 0.43352399680100717 1102 58-59 0.47275574695555633 1102 60-61 0.4298159459469346 1102 62-63 0.511226899235659 1102 64-65 0.5147452319219852 1102 66-67 0.4470815302902338 1102 68-69 0.40824160218884487 1102 70-71 0.5797124292229903 1102 72-73 0.5745167082458771 1102 74-75 0.5837137299483501 1102 76-77 0.513761006221003 1102 78-79 0.7437619519001082 1102 80-81 0.9151077979421061 1102 82-83 1.0878997625115332 1102 84-85 1.1673034446820232 1102 86-87 1.3974067452469079 1102 88-89 1.3522157304613955 1102 90-91 1.4610542823685684 1102 92-93 1.5560317008316424 1102 94-95 1.8403356709522924 1102 96-97 1.873256163446019 1102 98-99 2.202675597142683 1102 100-101 2.7090820606509247 1103 1 -0.0649497977346698 1103 2 -0.14928898118760614 1103 3 0.0463660692758765 1103 4 -0.03890066670441428 1103 5 0.04153503935980751 1103 6 0.05846534106389356 1103 7 -0.10736212098496623 1103 8 -0.06002490419722761 1103 9 -0.17713739009434448 1103 10-11 0.0011364650924079456 1103 12-13 0.04946093204046775 1103 14-15 0.11149593970184668 1103 16-17 0.23030170054563115 1103 18-19 0.06510643828672613 1103 20-21 0.15234127487432403 1103 22-23 -0.10998607821438355 1103 24-25 -0.13316581120623283 1103 26-27 -0.07183583670343552 1103 28-29 -0.002914520534851306 1103 30-31 -0.20998352910513063 1103 32-33 0.021475054731808996 1103 34-35 -0.017779351556661993 1103 36-37 0.09722834305015482 1103 38-39 0.22104288090610424 1103 40-41 0.2856956865865925 1103 42-43 0.21199269668836251 1103 44-45 0.447937695950813 1103 46-47 0.3440419227540872 1103 48-49 0.381924536164874 1103 50-51 0.28906257178239514 1103 52-53 0.5564019872356809 1103 54-55 0.588370930014662 1103 56-57 0.3673589569295572 1103 58-59 0.45691755082704333 1103 60-61 0.5461162306703855 1103 62-63 0.6899997997209155 1103 64-65 0.5925884844214835 1103 66-67 0.6936477586315704 1103 68-69 0.7470511376408098 1103 70-71 0.8179261320658178 1103 72-73 0.5779139272418341 1103 74-75 0.6943864780743603 1103 76-77 0.9082627595384629 1103 78-79 1.0187355298925986 1103 80-81 0.894508799049337 1103 82-83 0.9730871227460156 1103 84-85 1.2206012210697779 1103 86-87 1.1989855782051144 1103 88-89 1.4413502457125702 1103 90-91 1.6363891749594153 1103 92-93 1.6199903880923863 1103 94-95 1.744596743141141 1103 96-97 1.7070558054719989 1103 98-99 2.1282400937108505 1103 100-101 2.775328073142738 1104 1 0.07270625195321401 1104 2 0.12354367463048632 1104 3 0.0590404691007933 1104 4 0.06273250161860489 1104 5 0.13372000089865566 1104 6 0.22169615135447884 1104 7 0.187750235770622 1104 8 0.15493307368262066 1104 9 0.2505390524469959 1104 10-11 0.16427279821668606 1104 12-13 0.2013269112172651 1104 14-15 0.27597786784969003 1104 16-17 0.2344261006278785 1104 18-19 0.15340349900527883 1104 20-21 0.2437144189984508 1104 22-23 0.21479823568409984 1104 24-25 0.1953620531858249 1104 26-27 0.2799609817565525 1104 28-29 0.27345949728861996 1104 30-31 0.26616827744287974 1104 32-33 0.2715264596231961 1104 34-35 0.48509478843767084 1104 36-37 0.30461221762369206 1104 38-39 0.5143910511983236 1104 40-41 0.4759122880505373 1104 42-43 0.5390625982728139 1104 44-45 0.5102759604775642 1104 46-47 0.43693809761747815 1104 48-49 0.5407057292534105 1104 50-51 0.38782025218782934 1104 52-53 0.5159357381311267 1104 54-55 0.7023433554261018 1104 56-57 0.6015945655818484 1104 58-59 0.8215070187429205 1104 60-61 0.7179213033222283 1104 62-63 0.8360165272034337 1104 64-65 0.7870979763745041 1104 66-67 0.8819929524641594 1104 68-69 0.8370305291196303 1104 70-71 1.0393601459533492 1104 72-73 1.0446270850069403 1104 74-75 1.0325165801170506 1104 76-77 0.9109915688982468 1104 78-79 1.1921106880591381 1104 80-81 1.3356310211326807 1104 82-83 1.4436947984092114 1104 84-85 1.5995784218056954 1104 86-87 1.7302662204831911 1104 88-89 1.7873144777112415 1104 90-91 1.9894197108128253 1104 92-93 1.777144760351046 1104 94-95 1.9230448717457627 1104 96-97 2.263731566944479 1104 98-99 2.591736738974948 1104 100-101 3.3606657070864863 1105 1 -0.013239308298867769 1105 2 -0.01068785854186416 1105 3 -0.08393958288323944 1105 4 -0.08972325497642686 1105 5 -0.2004859553938516 1105 6 0.023788774765016285 1105 7 -0.05869108131052769 1105 8 -0.1093262457884947 1105 9 0.14265644196699157 1105 10-11 0.034106540139283936 1105 12-13 -0.009816500688359042 1105 14-15 0.18095822686773033 1105 16-17 0.06448124694407653 1105 18-19 0.22987864833608995 1105 20-21 0.17268940185665116 1105 22-23 0.07076710767405103 1105 24-25 -0.03683185522609023 1105 26-27 0.09320490188424202 1105 28-29 0.2372176436502258 1105 30-31 0.06036480539537337 1105 32-33 0.07813111768009406 1105 34-35 0.1376848375826114 1105 36-37 0.2797118156097511 1105 38-39 0.23283710808208014 1105 40-41 0.17164507262327078 1105 42-43 0.13081241069509275 1105 44-45 0.3217382753969389 1105 46-47 0.33338572533401134 1105 48-49 0.2508803959390207 1105 50-51 0.3523590784765105 1105 52-53 0.35910655797599844 1105 54-55 0.3707226700270212 1105 56-57 0.4498031670475271 1105 58-59 0.46000777337231114 1105 60-61 0.6462220372783705 1105 62-63 0.686647827564876 1105 64-65 0.5965791975674293 1105 66-67 0.6954058527402367 1105 68-69 0.6107382489073601 1105 70-71 0.6949467020989175 1105 72-73 0.521004830446774 1105 74-75 0.5383909040223251 1105 76-77 0.6381447702292462 1105 78-79 0.9274777455394165 1105 80-81 1.0347573394355614 1105 82-83 1.2332150480261852 1105 84-85 1.3465121913390625 1105 86-87 1.4270592753851616 1105 88-89 1.3674757067834022 1105 90-91 1.5131103654165656 1105 92-93 1.7057939026757971 1105 94-95 1.7574051808860993 1105 96-97 1.4782899659543922 1105 98-99 1.668764660959333 1105 100-101 2.3464944996878003 1106 1 -0.30971732577745215 1106 2 -0.27401567550910144 1106 3 -0.2684024547113282 1106 4 -0.2924727487609715 1106 5 -0.38009600492265605 1106 6 -0.1710153086624544 1106 7 -0.3452159418215075 1106 8 -0.21631840438599426 1106 9 -0.053445435703970645 1106 10-11 -0.5059652845271714 1106 12-13 -0.576100444771626 1106 14-15 -0.769792407053572 1106 16-17 -0.6130634566861985 1106 18-19 -0.1266415252385471 1106 20-21 -0.3087491452984068 1106 22-23 -0.3495720895959451 1106 24-25 -0.6889384093009667 1106 26-27 -0.5071376657669404 1106 28-29 -0.2657368706857426 1106 30-31 -0.17641006893942546 1106 32-33 -0.023977937849565478 1106 34-35 -0.15693667255881394 1106 36-37 -0.3453107303972729 1106 38-39 -0.5269791271494668 1106 40-41 -0.2798583017075913 1106 42-43 -0.11511482593463285 1106 44-45 0.011455354277025265 1106 46-47 0.1914597631296715 1106 48-49 0.155707775809951 1106 50-51 0.30902170331886225 1106 52-53 0.2717518069234117 1106 54-55 0.2700550778220787 1106 56-57 -0.07444777889735121 1106 58-59 -0.27947130524972863 1106 60-61 -0.07379127967701749 1106 62-63 -0.15714849871961434 1106 64-65 0.15277783545946733 1106 66-67 0.29883541308980455 1106 68-69 0.26025579727101444 1106 70-71 0.2681975269207193 1106 72-73 0.1317338662807721 1106 74-75 0.26683593389495996 1106 76-77 0.5792373201069374 1106 78-79 0.5686958947788412 1106 80-81 0.4660097915195358 1106 82-83 0.3386614063029718 1106 84-85 0.55668425851497 1106 86-87 0.688742550395105 1106 88-89 0.7627829117631428 1106 90-91 1.236682660093205 1106 92-93 1.300599697624964 1106 94-95 1.3343349545176046 1106 96-97 1.6225287864262974 1106 98-99 2.1555311422550325 1106 100-101 2.411853234636286 1107 1 -0.08899591545102226 1107 2 0.05026382444400568 1107 3 -0.0909551819639951 1107 4 0.018509084266177922 1107 5 0.06296807875686028 1107 6 0.11548552162282277 1107 7 -0.01848006632568655 1107 8 0.11585570174708693 1107 9 0.17205770062280834 1107 10-11 0.12927846350745398 1107 12-13 0.08391704362919938 1107 14-15 0.18179934084864158 1107 16-17 0.18142162852148402 1107 18-19 0.32900372503315367 1107 20-21 0.22692139971078262 1107 22-23 0.1349366077112819 1107 24-25 0.21722625199229384 1107 26-27 0.2886549118688677 1107 28-29 0.17703309615802 1107 30-31 0.22680997596769004 1107 32-33 0.28139533192970845 1107 34-35 0.3429657482330768 1107 36-37 0.26961861260158315 1107 38-39 0.4347077587566517 1107 40-41 0.4946916616053869 1107 42-43 0.5279783591058873 1107 44-45 0.5297847292735796 1107 46-47 0.5723027923193058 1107 48-49 0.5603963360787247 1107 50-51 0.4701832475943064 1107 52-53 0.5393408901891661 1107 54-55 0.7118437357390306 1107 56-57 0.7358872039728439 1107 58-59 0.6601047527433508 1107 60-61 0.9748628168567492 1107 62-63 0.904171930711648 1107 64-65 0.780895198076216 1107 66-67 1.014347541875054 1107 68-69 1.1516284160624934 1107 70-71 1.1507349173423727 1107 72-73 1.0508413923616615 1107 74-75 1.1245806197933526 1107 76-77 1.1710945248315667 1107 78-79 1.5884796482199732 1107 80-81 1.4323922027121085 1107 82-83 1.5917629079883682 1107 84-85 1.6545175863314583 1107 86-87 1.7119777804199856 1107 88-89 1.5336345196478192 1107 90-91 1.6662239205821088 1107 92-93 1.9211620848744282 1107 94-95 2.0761489899247216 1107 96-97 2.247206050953533 1107 98-99 2.2026522452931623 1107 100-101 2.5678206686961857 1108 1 0.05104728247361834 1108 2 0.008876465138659029 1108 3 -0.005945036116528968 1108 4 0.026140620784687485 1108 5 0.10636136724608036 1108 6 0.06374968840690087 1108 7 0.010465012466227108 1108 8 0.014518512459126498 1108 9 0.08912102610769068 1108 10-11 0.03201304900927937 1108 12-13 -0.030340305029177728 1108 14-15 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18-19 0.06794991550611229 2316 20-21 0.038651733965615165 2316 22-23 0.16734153085509007 2316 24-25 0.25958800932640713 2316 26-27 0.13681807251632705 2316 28-29 0.19930458227091918 2316 30-31 0.0034184203004414826 2316 32-33 -0.16613919052942094 2316 34-35 -0.6085788473803788 2316 36-37 -0.5490255530518837 2316 38-39 -0.7006982240041921 2316 40-41 -0.5309811272930887 2316 42-43 -1.1489759890598208 2316 44-45 -1.1641549065170267 2316 46-47 -1.148732643719903 2316 48-49 -1.0218292672577078 2316 50-51 -0.9066490068768047 2316 52-53 -0.9935233701505908 2316 54-55 -1.3192604395596703 2316 56-57 -1.4787667563458555 2316 58-59 -1.4150550388652903 2316 60-61 -1.285508354767245 2316 62-63 -1.2656894360584872 2316 64-65 -1.33463532364933 2316 66-67 -1.2941827149462313 2316 68-69 -1.246542216243597 2316 70-71 -1.3628847276859162 2316 72-73 -1.477470250520227 2316 74-75 -1.4636316500286206 2316 76-77 -1.3015040161572742 2316 78-79 -1.2976251044341645 2316 80-81 -1.4239770466578925 2316 82-83 -1.908375487506902 2316 84-85 -1.9935528578246853 2316 86-87 -1.9782243279462364 2316 88-89 -1.9479758605237585 2316 90-91 -1.964779726945789 2316 92-93 -1.8991479210349738 2316 94-95 -1.3981085989031143 2316 96-97 -1.4901065632439803 2316 98-99 -2.2039206067747905 2316 100-101 -2.654583439357582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6288.0 3 1383.0 4 1240.0 5 1447.0 6 1663.0 7 2030.0 8 2435.0 9 3067.0 10 3737.0 11 4992.0 12 6290.0 13 7958.0 14 8793.0 15 11430.0 16 13934.0 17 17672.0 18 15853.0 19 11912.0 20 11940.0 21 12754.0 22 13867.0 23 15229.0 24 17186.0 25 19405.0 26 22428.0 27 25445.0 28 30081.0 29 35781.0 30 43086.0 31 52176.0 32 64764.0 33 82607.0 34 108704.0 35 147019.0 36 200576.0 37 240070.0 38 140977.0 39 10706.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.710733698154314 18.74094263206581 14.822280806383754 22.72604286339612 2 14.992414701674427 19.784001285571794 41.18232961749283 24.041254395260943 3 19.29254599902303 24.331023057917353 29.819402065796837 26.557028877262784 4 12.420149055097152 16.12139608900266 36.94210523335165 34.516349622548546 5 13.435369454431676 37.568037648737786 34.152722886346595 14.84387001048394 6 32.049925448511374 35.71709478365922 17.429204378591415 14.803775389237991 7 28.55061516743474 32.95127602332233 20.336665902834948 18.16144290640798 8 26.429567741606924 35.13301890529246 19.415412821194753 19.022000531905864 9 26.47477811132205 16.79351408855176 20.367711342422965 36.36399645770322 10-11 25.022351489910704 26.71385000169758 26.83031116669119 21.433487341700523 12-13 25.73733292590455 24.083921841578107 27.82420296743965 22.354542265077697 14-15 22.767867089303707 25.28639577202638 26.4521016266905 25.493635511979413 16-17 21.8390032314211 28.753706869298245 27.015394690963973 22.391895208316683 18-19 21.68598185698096 26.998940979496727 29.811409487338608 21.503667676183706 20-21 22.378864158557 25.965009798076878 30.096467388421793 21.559658654944332 22-23 22.14516402233902 25.805825359448093 30.055338333315063 21.993672284897823 24-25 21.899576506133165 26.009546292943465 30.16728997872629 21.923587222197078 26-27 21.560504801384575 26.28613946330019 30.1679865729565 21.98536916235873 28-29 21.503771524284314 26.573932344569627 30.150196073993175 21.772100057152883 30-31 21.63294899908043 26.20821249204216 30.12633514890005 22.032503359977365 32-33 21.48349771134594 26.19046440119034 30.131858446516375 22.194179440947348 34-35 21.956035514510532 26.308593142123115 29.95196402146942 21.783407321896934 36-37 21.918897166676803 26.081573851557117 29.9449842215964 22.05454476016968 38-39 21.621130433798736 26.440126383200198 29.982667568056844 21.956075614944222 40-41 21.85956147654732 26.32653963169404 29.92799701593329 21.885901875825347 42-43 21.513681063834827 26.32518786025481 29.699618073763638 22.46151300214672 44-45 21.686663484149296 26.525610424512458 29.546915610247705 22.24081048109054 46-47 21.94272781122634 26.732161012828332 29.05646765509376 22.26864352085157 48-49 21.99400805174678 26.77274770545022 28.94038149420982 22.292862748593173 50-51 21.964528414791697 27.00152322197344 28.77199025732089 22.26195810591397 52-53 22.10936076814647 27.140656475885766 28.544515248146045 22.205467507821716 54-55 21.893073856093114 27.025385887989746 28.57031709195745 22.51122316395969 56-57 21.950163195347944 27.380438902150672 28.41554145784073 22.253856444660656 58-59 22.136159876369966 26.917562774691188 28.603336908103888 22.342940440834955 60-61 22.078377996181857 27.05472671993212 28.834971363925614 22.031923919960406 62-63 21.8649658633878 27.080922842736854 28.990119772439083 22.063991521436265 64-65 21.771175052474273 26.984520929430104 28.838912381665523 22.405391636430103 66-67 21.86932567045474 27.22571599079097 28.42008281339269 22.484875525361602 68-69 21.767913846388804 27.05094565481001 28.767081639045443 22.41405885975574 70-71 21.892362756008954 27.04598580505209 28.608024006120313 22.45362743281864 72-73 21.8940424138665 27.057915456544134 28.523887412109218 22.524154717480144 74-75 21.678113967593013 27.49063659817145 28.332847718837694 22.498401715397844 76-77 21.92304550628487 27.18898946705125 28.326620068083862 22.561344958580023 78-79 21.92701038612129 27.15390999119197 28.30913747422741 22.609942148459336 80-81 21.912452164652247 27.41696016994609 28.167970267920378 22.502617397481288 82-83 22.071392233039226 27.343842526844824 28.19816083810518 22.386604402010768 84-85 21.865261748297556 27.290207015235463 28.067800061441023 22.776731175025958 86-87 21.856267842780248 27.31678171485138 28.317409250404364 22.509541191964008 88-89 21.926227696842172 27.205158821218685 28.106283653844454 22.762329828094686 90-91 21.931431109011722 27.065470958562944 28.23598169667133 22.767116235754 92-93 22.024155491163537 26.889688839487107 28.300998264809575 22.78515740453978 94-95 21.85594054767835 26.8811395499711 28.27612661902043 22.986793283330123 96-97 21.734255656027003 26.917618206774435 28.263552153968114 23.084573983230452 98-99 21.681066259341172 26.937439117744294 28.065858026557482 23.315636596357052 100-101 22.005858849588336 26.18761539448609 28.76396796898281 23.042557786942762 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1886.0 1 1115.5 2 345.5 3 354.0 4 314.0 5 312.5 6 528.5 7 1119.0 8 1874.0 9 2268.0 10 2096.5 11 1878.5 12 1981.5 13 2129.0 14 2283.5 15 2424.5 16 2550.5 17 2615.0 18 2735.5 19 2932.0 20 3076.0 21 3468.5 22 4101.0 23 4916.5 24 5839.5 25 7030.5 26 8861.5 27 10879.5 28 12440.5 29 14007.0 30 16187.5 31 18643.0 32 21318.5 33 24059.5 34 27027.0 35 30001.0 36 33331.5 37 36737.5 38 39080.0 39 40628.0 40 41572.0 41 43347.0 42 45280.5 43 46995.0 44 49360.5 45 51307.0 46 52368.0 47 54142.0 48 57063.0 49 57054.0 50 54275.0 51 51741.0 52 49948.5 53 49027.0 54 47875.5 55 43901.0 56 38662.0 57 34220.0 58 30356.0 59 26707.5 60 22408.0 61 18414.5 62 14665.0 63 11047.5 64 8227.5 65 6250.0 66 4567.5 67 3398.5 68 2484.0 69 1692.0 70 1233.0 71 886.5 72 636.5 73 433.0 74 299.5 75 203.5 76 150.5 77 95.5 78 54.0 79 32.0 80 20.5 81 15.5 82 12.0 83 12.0 84 11.0 85 8.0 86 5.5 87 4.0 88 3.5 89 3.5 90 3.5 91 3.5 92 3.0 93 1.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3593695083582346 2 0.305238241093748 3 0.3102490885197957 4 0.3030504063020934 5 0.3026975297227943 6 0.2214653411681344 7 0.21186709821119803 8 0.2217476424315737 9 0.22259454622189165 10-11 0.22231224495845228 12-13 0.22457065506596674 14-15 0.2191010680868302 16-17 0.21151422163189892 18-19 0.20350392328180866 20-21 0.2027628824652805 22-23 0.21084375613123055 24-25 0.2098909893671229 26-27 0.21617219247864744 28-29 0.22400605253908812 30-31 0.2276759689637991 32-33 0.22675848985762134 34-35 0.22541755885628467 36-37 0.20946753747196398 38-39 0.19859893882955074 40-41 0.19397625564073212 42-43 0.20533888149416413 44-45 0.2082677571023469 46-47 0.21620748013657737 48-49 0.2255234218300744 50-51 0.2220652313529429 52-53 0.23949733437032 54-55 0.21941865700819946 56-57 0.18843609334573577 58-59 0.16818097769396567 60-61 0.1853307794479034 62-63 0.17894371336258916 64-65 0.18783620316092725 66-67 0.18787149081885715 68-69 0.18455445097344533 70-71 0.18490732755274444 72-73 0.1985283635136909 74-75 0.20537416915209403 76-77 0.20710326439065976 78-79 0.20293932075493 80-81 0.19976343154123788 82-83 0.1863541215278709 84-85 0.18077867157494462 86-87 0.1879773537926469 88-89 0.1979284733288824 90-91 0.19457614582554064 92-93 0.18857724397745543 94-95 0.18938886010984343 96-97 0.19866951414541056 98-99 0.19926940433021909 100-101 0.175344372253738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1416926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.21347158590979 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1977229852988 48.28983314229953 2 11.616710682719603 13.989649572313429 3 3.503576790752558 6.328875646170965 4 1.5103228344040338 3.637671242997521 5 0.802049841244154 2.4147102663119155 6 0.48437154362921137 1.7499415307604465 7 0.33311778255847446 1.404072469439218 8 0.22481470786797597 1.0829499219442382 9 0.1787688634733835 0.9687864493079966 >10 1.0375218848861227 11.717534896120242 >50 0.07657473665751881 3.1677500173398663 >100 0.03315852252772297 3.4736200665320447 >500 5.858290820148429E-4 0.25722386190165974 >1k 4.686632656118743E-4 0.6027758299747068 >5k 2.3433163280593716E-4 0.914605086586154 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7161 0.505389836872215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5803 0.40954855793457107 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3824 0.2698800078479751 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2084 0.14707895825187767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.057531585982614E-5 0.0 18-19 0.0 0.0 0.0 2.1172594757947838E-4 0.0 20-21 0.0 0.0 0.0 2.1172594757947838E-4 0.0 22-23 0.0 0.0 0.0 3.175889213692176E-4 0.0 24-25 0.0 3.528765792991307E-5 0.0 3.881642372290437E-4 0.0 26-27 0.0 7.057531585982614E-5 0.0 5.998901848085221E-4 0.0 28-29 0.0 7.057531585982614E-5 0.0 0.0012350680275469573 0.0 30-31 0.0 7.057531585982614E-5 0.0 0.0028230126343930452 0.0 32-33 0.0 7.057531585982614E-5 0.0 0.0046932585046784376 0.0 34-35 0.0 7.057531585982614E-5 0.0 0.006316490769454439 0.0 36-37 0.0 7.057531585982614E-5 0.0 0.008222024297669744 0.0 38-39 0.0 7.057531585982614E-5 0.0 0.013197584065787487 0.0 40-41 0.0 7.057531585982614E-5 0.0 0.017361527701517228 0.0 42-43 0.0 7.057531585982614E-5 0.0 0.02117259475794784 0.0 44-45 0.0 7.057531585982614E-5 0.0 0.023536867839252015 0.0 46-47 0.0 7.057531585982614E-5 0.0 0.026889195342593757 0.0 48-49 0.0 7.057531585982614E-5 0.0 0.031264864925902976 0.0 50-51 0.0 7.057531585982614E-5 0.0 0.036169849378160895 0.0 52-53 0.0 1.058629737897392E-4 0.0 0.04848524199570055 0.0 54-55 0.0 1.4115063171965227E-4 0.0 0.056248526740281427 0.0 56-57 0.0 1.4115063171965227E-4 0.0 0.07523328670657466 0.0 58-59 0.0 1.4115063171965227E-4 0.0 0.10603941207938876 0.0 60-61 0.0 1.4115063171965227E-4 0.0 0.13384608652816027 0.0 62-63 0.0 1.4115063171965227E-4 0.0 0.1476435607787563 0.0 64-65 0.0 1.4115063171965227E-4 0.0 0.17135686690765783 0.0 66-67 3.528765792991307E-5 1.4115063171965227E-4 0.0 0.22707607877899058 0.0 68-69 7.057531585982614E-5 1.4115063171965227E-4 0.0 0.31024908851979566 0.0 70-71 7.057531585982614E-5 1.4115063171965227E-4 0.0 0.3886229767821326 0.0 72-73 7.057531585982614E-5 1.4115063171965227E-4 0.0 0.47783017602895284 0.0 74-75 7.057531585982614E-5 1.4115063171965227E-4 0.0 0.5821404928697759 0.0 76-77 7.057531585982614E-5 1.4115063171965227E-4 0.0 0.6531745482826908 0.0 78-79 7.057531585982614E-5 1.4115063171965227E-4 0.0 0.760660754337206 0.0 80-81 7.057531585982614E-5 1.4115063171965227E-4 0.0 0.914514942911627 0.0 82-83 7.057531585982614E-5 1.4115063171965227E-4 0.0 1.0437736339088985 0.0 84-85 7.057531585982614E-5 1.4115063171965227E-4 0.0 1.2064497369657978 0.0 86-87 7.057531585982614E-5 2.1172594757947838E-4 0.0 1.4019433618975161 0.0 88-89 7.057531585982614E-5 2.1172594757947838E-4 0.0 1.6734466020102672 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4870 0.0 38.58297 1 GTATCAA 7600 0.0 37.67996 1 ATCAACG 9245 0.0 30.54214 3 TCAACGC 9305 0.0 30.349495 4 TATCAAC 9420 0.0 30.025204 2 CAACGCA 9465 0.0 29.788338 5 AACGCAG 9665 0.0 29.151276 6 GTACATG 6630 0.0 26.188274 1 TACATGG 6645 0.0 25.538368 2 ACGCAGA 11045 0.0 25.207005 7 CGCAGAG 11050 0.0 25.066612 8 ACATGGG 6640 0.0 23.98262 3 GAGTACT 6010 0.0 23.788029 12-13 CATGGGG 3310 0.0 23.412134 4 GCAGAGT 11600 0.0 23.384966 9 ACACCGT 260 6.2391337E-10 21.928686 6 GTACTTT 6570 0.0 21.578175 14-15 AGAGTAC 9550 0.0 21.561695 10-11 AGTACTT 6330 0.0 21.3474 12-13 GTACCTG 1115 0.0 20.478298 1 >>END_MODULE