FastQCFastQC Report
Thu 26 May 2016
SRR937724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416926
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83490.5892333121136883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72240.509836081771384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48480.3421491312884371No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18830.1328933197640526No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17250.12174241985820008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59950.034.9640121
GTATCAA86800.031.102871
TAACGCA1257.3820665E-626.6048324
ATCAACG109450.024.6115993
TCAACGC109900.024.597284
TATCAAC110550.024.409682
CAACGCA111200.024.3524445
AACGCAG113900.023.853546
GTATAGG4300.023.2125381
GTACATG67700.023.0280421
GCACCGT2354.652975E-922.2333746
GAGTACT69450.022.22833612-13
TACATGG68200.022.2217772
ACATGGG66750.021.5657143
GTACTTT72700.021.1685514-15
ACGCAGA127950.021.0864727
AGTACTT73200.020.99225412-13
CGCAGAG127850.020.9543518
GTACCTG9150.020.7783361
CCGTCCG1150.003723587120.6522839
Produced by FastQC (version 0.11.5)