##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937724_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1416926 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29229684542453 33.0 31.0 34.0 31.0 34.0 2 32.53757006364482 34.0 31.0 34.0 31.0 34.0 3 32.624978298090376 34.0 31.0 34.0 31.0 34.0 4 36.22034107638649 37.0 35.0 37.0 35.0 37.0 5 36.067367667754 37.0 35.0 37.0 35.0 37.0 6 36.16590280649801 37.0 35.0 37.0 35.0 37.0 7 36.12908154695447 37.0 35.0 37.0 35.0 37.0 8 36.123104523454295 37.0 35.0 37.0 35.0 37.0 9 37.81936177330362 39.0 38.0 39.0 35.0 39.0 10-11 37.76355257790456 39.0 37.0 39.0 35.0 39.0 12-13 37.6764037077448 39.0 37.0 39.0 35.0 39.0 14-15 38.92270944283611 40.0 38.0 41.0 35.5 41.0 16-17 38.89055215304116 40.0 38.0 41.0 35.5 41.0 18-19 38.88329065879235 40.0 38.0 41.0 35.0 41.0 20-21 38.805803549373785 40.0 38.0 41.0 35.0 41.0 22-23 38.660100456904594 40.0 38.0 41.0 34.5 41.0 24-25 38.605796632992835 40.0 38.0 41.0 34.0 41.0 26-27 38.5321103572099 40.0 38.0 41.0 34.0 41.0 28-29 38.423646683030725 40.0 38.0 41.0 34.0 41.0 30-31 38.487594976731316 40.0 38.0 41.0 34.0 41.0 32-33 38.39812594306265 40.0 38.0 41.0 34.0 41.0 34-35 38.26713533381419 40.0 38.0 41.0 34.0 41.0 36-37 38.11667758231552 40.0 38.0 41.0 34.0 41.0 38-39 37.98517671353338 40.0 37.0 41.0 33.5 41.0 40-41 37.80630428123981 40.0 37.0 41.0 33.0 41.0 42-43 37.59123341656516 40.0 37.0 41.0 33.0 41.0 44-45 37.27645233413742 40.0 37.0 41.0 32.5 41.0 46-47 36.88567010556656 40.0 36.5 41.0 31.5 41.0 48-49 36.52120153063745 40.0 36.0 41.0 30.5 41.0 50-51 36.25083102434425 39.0 36.0 41.0 30.0 41.0 52-53 36.02701376077509 39.0 35.5 41.0 29.5 41.0 54-55 35.80590658933494 39.0 35.0 40.0 29.0 41.0 56-57 35.521894933115775 39.0 35.0 40.0 28.0 41.0 58-59 35.259066810828514 38.0 35.0 40.0 28.0 41.0 60-61 34.910578604669546 38.0 34.0 40.0 27.0 41.0 62-63 34.540365904782604 37.5 34.0 40.0 26.0 41.0 64-65 34.13501234362275 37.0 34.0 39.5 26.0 41.0 66-67 33.703127051095116 36.0 33.0 39.0 25.5 40.5 68-69 33.24404803073696 36.0 33.0 39.0 24.5 40.0 70-71 32.82853585861224 35.0 32.0 38.5 23.5 40.0 72-73 32.38193102533231 35.0 32.0 37.5 22.0 39.5 74-75 31.88031238046306 35.0 31.5 37.0 21.0 39.0 76-77 30.67170762622748 34.0 30.0 35.5 20.0 38.0 78-79 31.224099917709182 34.0 31.0 36.0 20.0 38.5 80-81 31.025331245244985 34.0 31.0 36.0 19.5 37.0 82-83 30.678362878513063 34.0 31.0 35.0 18.0 37.0 84-85 30.293971597669884 34.0 31.0 35.0 16.0 36.5 86-87 29.89008847321596 34.0 30.0 35.0 9.0 36.0 88-89 29.452324256877212 34.0 29.5 35.0 6.0 36.0 90-91 28.951482646235583 34.0 29.0 35.0 2.0 35.0 92-93 28.50083984625873 34.0 29.0 35.0 2.0 35.0 94-95 28.0327854101061 33.0 28.5 35.0 2.0 35.0 96-97 27.504276158387945 33.0 27.0 35.0 2.0 35.0 98-99 26.71553560312959 33.0 25.5 35.0 2.0 35.0 100-101 24.326661025346418 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18243900849302008 1101 2 0.1420041425330112 1101 3 0.15123019140254712 1101 4 0.07015618339004703 1101 5 0.05867985315679647 1101 6 0.0706648522258746 1101 7 0.05885320977549213 1101 8 -0.013503287085455895 1101 9 0.22799307716719852 1101 10-11 0.060924332279405746 1101 12-13 0.10659435519917082 1101 14-15 0.14265808153806603 1101 16-17 0.07012853324189194 1101 18-19 0.23820203390642547 1101 20-21 0.15515225857215853 1101 22-23 0.21122139537532547 1101 24-25 0.2095170918054805 1101 26-27 0.2152924225110482 1101 28-29 0.22049210349728554 1101 30-31 0.19094206680706094 1101 32-33 0.18929613959883085 1101 34-35 0.18454560545394116 1101 36-37 0.36323258932161906 1101 38-39 0.3195931898885789 1101 40-41 0.22091855515710535 1101 42-43 0.22760821949641752 1101 44-45 0.26329586645254466 1101 46-47 0.276435870403958 1101 48-49 0.2255095038428223 1101 50-51 0.31721260398038 1101 52-53 0.1317184529128852 1101 54-55 0.2728392330985514 1101 56-57 0.2298779451935289 1101 58-59 0.2753978493363576 1101 60-61 0.255003655779106 1101 62-63 0.355180547166448 1101 64-65 0.22857168094942182 1101 66-67 0.3748902747072336 1101 68-69 0.38048991669823096 1101 70-71 0.30375693064497256 1101 72-73 0.37449080112478583 1101 74-75 0.39227209261150975 1101 76-77 0.3017002845473442 1101 78-79 0.3311191861772933 1101 80-81 0.4442583803113429 1101 82-83 0.42237718191850604 1101 84-85 0.6106985247315571 1101 86-87 0.8324027199515172 1101 88-89 0.8809602858314207 1101 90-91 0.7532823325382125 1101 92-93 0.9217399880055765 1101 94-95 0.8855973214174035 1101 96-97 1.06498753873203 1101 98-99 1.5933367328848185 1101 100-101 2.2489371151838142 1102 1 -0.03228885441933471 1102 2 0.07301542445441811 1102 3 0.011086713999262088 1102 4 -0.02822202518791528 1102 5 -0.10899828212398432 1102 6 -0.13412649432367374 1102 7 -0.1389824535639832 1102 8 -0.1545728414824339 1102 9 -0.08783156480895116 1102 10-11 -0.10777302342133765 1102 12-13 -0.07471108949068395 1102 14-15 -0.018027965454926687 1102 16-17 -0.0970652562223151 1102 18-19 -0.054573567142625734 1102 20-21 -0.11259953915684662 1102 22-23 0.025738011432039798 1102 24-25 -0.12971857202101944 1102 26-27 -0.16990507807946642 1102 28-29 -0.19591392391731688 1102 30-31 -0.03479218343097301 1102 32-33 -0.0413954107704555 1102 34-35 -0.05263749382356053 1102 36-37 0.04589439710768062 1102 38-39 -0.014828724263622917 1102 40-41 -0.020868751524623974 1102 42-43 0.02980628260016971 1102 44-45 0.0722163280543171 1102 46-47 0.09128268903849346 1102 48-49 0.08399249573142953 1102 50-51 0.27917952527966605 1102 52-53 0.0780689351888455 1102 54-55 0.1685143342170008 1102 56-57 0.21228211704497113 1102 58-59 0.1974736936541177 1102 60-61 0.2048549915654121 1102 62-63 0.2678095782069505 1102 64-65 0.21825041527028333 1102 66-67 0.3248216042596326 1102 68-69 0.023731843659177798 1102 70-71 0.11063128077172024 1102 72-73 0.2027562391911033 1102 74-75 0.13706531420519852 1102 76-77 0.1898117741846832 1102 78-79 0.34283102842033486 1102 80-81 0.2712301596795257 1102 82-83 0.27530066794212615 1102 84-85 0.37520917473873894 1102 86-87 0.4874210198952653 1102 88-89 0.4891974521283551 1102 90-91 0.7324432868139183 1102 92-93 0.8052852581458652 1102 94-95 0.8848359804442829 1102 96-97 0.9943362409336238 1102 98-99 1.2634737328629022 1102 100-101 1.967613894101497 1103 1 0.07363858346171526 1103 2 0.06671214773817269 1103 3 -0.019558699513019917 1103 4 -0.055343858843762916 1103 5 -0.06239726823542924 1103 6 -0.0412241499666095 1103 7 -0.05223983804909693 1103 8 -0.04522712446200927 1103 9 0.015196109187776585 1103 10-11 0.010696698144194272 1103 12-13 0.05534050059855389 1103 14-15 0.04829635688049905 1103 16-17 -0.11591190594081269 1103 18-19 0.02562358000677989 1103 20-21 -0.028405322030160107 1103 22-23 0.07459451826592556 1103 24-25 -0.08073509915011101 1103 26-27 -0.1264181816038743 1103 28-29 -0.14255514573485328 1103 30-31 -0.049615982651801005 1103 32-33 0.00534915099216704 1103 34-35 0.11120848535933447 1103 36-37 0.08592573556936145 1103 38-39 -0.026691152628878 1103 40-41 -0.102039990824494 1103 42-43 0.0024211971516621134 1103 44-45 -0.04428100214888531 1103 46-47 0.004860696051764535 1103 48-49 -0.01785593126951568 1103 50-51 0.03945929597101383 1103 52-53 0.031097432225656974 1103 54-55 -0.05945457713819735 1103 56-57 3.392486147149043E-4 1103 58-59 0.023972243893211953 1103 60-61 -0.2048628364027465 1103 62-63 -0.06462478555643969 1103 64-65 0.061374133203791814 1103 66-67 0.09318290239718152 1103 68-69 -0.15654007910286794 1103 70-71 0.11526717509918427 1103 72-73 0.168479766469801 1103 74-75 0.09212115263371246 1103 76-77 -0.07901538884939185 1103 78-79 0.1359694642471716 1103 80-81 0.24999875130785298 1103 82-83 0.21227007941520526 1103 84-85 0.36470149456437184 1103 86-87 0.3808171156835769 1103 88-89 0.5434739200082497 1103 90-91 0.5644266730235472 1103 92-93 0.7031364215130296 1103 94-95 0.6121772026975414 1103 96-97 0.9589119903963486 1103 98-99 1.0801317495396319 1103 100-101 1.5243807844575485 1104 1 0.05622096424219336 1104 2 0.10885435560215484 1104 3 -0.04373676654696368 1104 4 -0.01600141011815026 1104 5 -0.058440255713712475 1104 6 0.007806680959454582 1104 7 0.03469238910489025 1104 8 0.042243046609058865 1104 9 0.05415133632386926 1104 10-11 0.04161744491328534 1104 12-13 0.06564316818769811 1104 14-15 0.017535645363274455 1104 16-17 -0.12066431572123548 1104 18-19 -0.03589992618178428 1104 20-21 0.06967214728559412 1104 22-23 0.11496727051199684 1104 24-25 0.004171934583013126 1104 26-27 0.04367024951394427 1104 28-29 0.08006295746263703 1104 30-31 0.0957368313585647 1104 32-33 0.007052435832115123 1104 34-35 -0.025906924564957023 1104 36-37 0.07879816247577054 1104 38-39 -0.06387937491661688 1104 40-41 0.0728921412396275 1104 42-43 0.2897421898799877 1104 44-45 0.16095759129564868 1104 46-47 0.2775521875435487 1104 48-49 0.30319635075229456 1104 50-51 0.39073520336231127 1104 52-53 0.43582168266392074 1104 54-55 0.38238216680858983 1104 56-57 0.4002103626438682 1104 58-59 0.3528642926409731 1104 60-61 0.4041301154290977 1104 62-63 0.506277203022492 1104 64-65 0.3803691167235925 1104 66-67 0.40320794681125705 1104 68-69 0.5342868865184656 1104 70-71 0.49679954817198535 1104 72-73 0.6372068441158163 1104 74-75 0.608318288615397 1104 76-77 0.2625970116524279 1104 78-79 0.5005237636199489 1104 80-81 0.5437586508851204 1104 82-83 0.555543856597609 1104 84-85 0.5228641134175014 1104 86-87 0.48332704681450167 1104 88-89 0.5096309981615761 1104 90-91 0.41016347360761074 1104 92-93 0.7216745325725924 1104 94-95 0.9326848011860065 1104 96-97 0.8946377404604995 1104 98-99 0.9987239245755539 1104 100-101 1.7896868818986782 1105 1 0.006374467313506216 1105 2 -8.676727502887616E-4 1105 3 -0.032922594280826445 1105 4 -0.07986476159769751 1105 5 -0.21304330003108873 1105 6 -0.1485644557266781 1105 7 -0.10140753792577328 1105 8 -0.0728331309791912 1105 9 -0.07118651949217991 1105 10-11 0.02233058293175816 1105 12-13 -0.06330456609916979 1105 14-15 -0.08313542732493318 1105 16-17 -0.11532103248014636 1105 18-19 -0.021753376232624078 1105 20-21 -0.028128631109687774 1105 22-23 -0.04066205307425719 1105 24-25 -0.114227705000296 1105 26-27 -0.1406528226983923 1105 28-29 -0.2499672527887853 1105 30-31 -0.18100640156462333 1105 32-33 -0.18892190816131205 1105 34-35 -0.17880233602256368 1105 36-37 -0.024717014671978177 1105 38-39 -0.11866349128417397 1105 40-41 -0.1042928082989647 1105 42-43 -0.20689589922061202 1105 44-45 -0.21388077703314679 1105 46-47 -0.25263368504737116 1105 48-49 -0.40152344728507927 1105 50-51 -0.441275822000307 1105 52-53 -0.3382552023284475 1105 54-55 -0.3462570289517757 1105 56-57 -0.25442820511772624 1105 58-59 -0.20130756671738936 1105 60-61 -0.3046029959593284 1105 62-63 -0.22318293256841315 1105 64-65 -0.1996865171692832 1105 66-67 -0.20704860489596655 1105 68-69 -0.3394636285714725 1105 70-71 -0.32271781220485707 1105 72-73 -0.22797820238088917 1105 74-75 -0.037893282331605604 1105 76-77 -0.20442817711636962 1105 78-79 -0.11569849779415264 1105 80-81 0.03517771914557599 1105 82-83 0.015750076460143703 1105 84-85 0.08403687743958344 1105 86-87 0.2746560666265552 1105 88-89 0.23325456221341057 1105 90-91 0.25552657753750907 1105 92-93 0.3421637865217555 1105 94-95 0.3406187604563584 1105 96-97 0.4154975248290107 1105 98-99 0.7585932428792646 1105 100-101 1.1658729121858258 1106 1 -0.24212762205952743 1106 2 -0.08252404395565094 1106 3 -0.17379893288827475 1106 4 -0.06609919004160503 1106 5 -0.1720197705573696 1106 6 -0.23828999347362867 1106 7 -0.2518019120222874 1106 8 -0.3223055215010433 1106 9 -0.238675045714011 1106 10-11 -0.3230080877161683 1106 12-13 -0.39904061258936707 1106 14-15 -0.2875136644049192 1106 16-17 -0.24311311682393466 1106 18-19 -0.10440753167564765 1106 20-21 -0.2630476563849129 1106 22-23 -0.28928361982755746 1106 24-25 -0.2561220698809308 1106 26-27 -0.29376275637034865 1106 28-29 -0.18346443141108892 1106 30-31 -0.3435565294021288 1106 32-33 -0.3859371459706651 1106 34-35 -0.5683397380379489 1106 36-37 -0.3881412034765219 1106 38-39 -0.35347455903673364 1106 40-41 -0.27963902939070806 1106 42-43 -0.2791296851856089 1106 44-45 -0.2851668078563421 1106 46-47 0.02786266941140525 1106 48-49 -0.05462350679336936 1106 50-51 -0.2158540964704656 1106 52-53 -0.2146142163866145 1106 54-55 -0.3280742502831302 1106 56-57 -0.4828809697518963 1106 58-59 -0.48624400343453544 1106 60-61 -0.2688883105765143 1106 62-63 -0.15934300855997208 1106 64-65 -0.32931503138413376 1106 66-67 -0.11188190926509378 1106 68-69 -0.2685663106576328 1106 70-71 -0.18612587320875917 1106 72-73 0.08371606586458569 1106 74-75 0.15568426540010094 1106 76-77 -0.055350851882483454 1106 78-79 0.18691433756535858 1106 80-81 0.0774590524999752 1106 82-83 0.0811402441629383 1106 84-85 0.11931989730113557 1106 86-87 0.035048953015746065 1106 88-89 0.04730381849140031 1106 90-91 0.20951478515299016 1106 92-93 0.42230397989737867 1106 94-95 0.186297760611712 1106 96-97 0.35216513283261364 1106 98-99 0.6034052914654175 1106 100-101 1.2530762593956517 1107 1 -0.023517784420292287 1107 2 0.05133049337408835 1107 3 -0.018169405626785817 1107 4 0.007515530006060089 1107 5 -0.2191550105125728 1107 6 -0.08326731923784791 1107 7 0.012441893939211468 1107 8 0.007659087933866715 1107 9 0.03401780941914012 1107 10-11 0.010345244182779822 1107 12-13 0.032835997639971026 1107 14-15 -0.03706231746704702 1107 16-17 -0.03582315693140714 1107 18-19 0.06218914001134124 1107 20-21 0.014621048756232824 1107 22-23 0.09922368723455577 1107 24-25 0.02984086794973706 1107 26-27 -0.22824780020167879 1107 28-29 -0.18463469230271556 1107 30-31 -0.1633080782565557 1107 32-33 -0.04957230593161199 1107 34-35 0.04761401424465106 1107 36-37 -0.13289399362123788 1107 38-39 -0.18324918209825114 1107 40-41 -0.06811040799527746 1107 42-43 -0.19654319483733218 1107 44-45 -0.15890586381370753 1107 46-47 0.10527160008120973 1107 48-49 -0.03181307388648946 1107 50-51 0.05937202524219032 1107 52-53 0.10910325491199302 1107 54-55 -0.1128521006993779 1107 56-57 -0.089253685088174 1107 58-59 -0.06766781855399984 1107 60-61 -0.07491429216128154 1107 62-63 -0.17131215008361522 1107 64-65 -0.105782443376647 1107 66-67 0.04736011751368352 1107 68-69 0.0838397926287513 1107 70-71 0.17792525691708505 1107 72-73 0.17494159175790713 1107 74-75 0.20763950447260626 1107 76-77 0.0836450408259033 1107 78-79 0.28075864114634186 1107 80-81 0.29400455544321247 1107 82-83 0.1783094816796975 1107 84-85 0.12619802466303298 1107 86-87 0.320134712381968 1107 88-89 0.3765322738297776 1107 90-91 0.47521055593908557 1107 92-93 0.7000768155008927 1107 94-95 0.7208973479516523 1107 96-97 0.8240571488535338 1107 98-99 1.0796476851144234 1107 100-101 1.6247199484000276 1108 1 0.012618717535524127 1108 2 0.13396431761053407 1108 3 0.046783627160934316 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90-91 -0.0030774832939144403 2316 92-93 -0.11266813603521086 2316 94-95 -0.046968406719830824 2316 96-97 0.13333117533037608 2316 98-99 0.28500112853019743 2316 100-101 0.04932581840674999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 8.0 5 28.0 6 48.0 7 101.0 8 285.0 9 484.0 10 959.0 11 1652.0 12 2589.0 13 3866.0 14 4996.0 15 7274.0 16 10286.0 17 15361.0 18 14323.0 19 8414.0 20 6805.0 21 6702.0 22 6896.0 23 7379.0 24 8315.0 25 9996.0 26 12040.0 27 14922.0 28 18667.0 29 23845.0 30 29624.0 31 38031.0 32 49314.0 33 65994.0 34 93098.0 35 136259.0 36 212993.0 37 329630.0 38 253971.0 39 21763.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45429820932068 19.593118572314157 15.214984601089856 21.737598617275303 2 15.824044445510916 20.63932767131099 40.208239526976 23.328388356202087 3 19.815360858647523 24.60544869668564 30.05252215006288 25.526668294603954 4 13.13441915809294 16.810687361231285 36.453209271338096 33.60168420933768 5 13.563125409410222 37.96538648325088 33.86069605385015 14.610792053488739 6 32.35405377556767 36.21099478730717 16.87731045940296 14.557640977722198 7 28.762546526776983 33.41903529189245 19.911625589480327 17.906792591850245 8 26.756866625356583 35.587885323580764 18.933451711663135 18.721796339399514 9 26.859553709932626 16.923184414711848 20.187433923860524 36.029827951494994 10-11 25.377472076876277 27.1319038538357 26.30885452027841 21.181769549009616 12-13 26.24456057798413 24.34112017774572 27.59208684301257 21.822232401257573 14-15 23.03521849412037 25.506095590030814 26.353458119901816 25.105227795947 16-17 21.883217613340427 29.17470637139837 26.90345155639744 22.038624458863765 18-19 21.819135226539707 27.17160246900685 29.972525029535767 21.036737274917673 20-21 22.545398470138338 26.04039520779321 30.45022480010417 20.963981521964282 22-23 22.363851747504533 25.926084067525313 30.334305982586883 21.375758202383267 24-25 22.29828321106683 26.133789628690714 30.47878194829131 21.089145211951145 26-27 21.80449243996453 26.504922924585262 30.440651242526336 21.249933392923875 28-29 21.676842759776214 26.70788625454607 30.378831561956776 21.23643942372094 30-31 21.867151757247687 26.357392014707987 30.355331203122272 21.420125024922058 32-33 21.826863223626358 26.353916859454905 30.30288808307561 21.51633183384312 34-35 22.23934065716911 26.437795622354308 30.267388699198122 21.055475021278458 36-37 22.255361253869292 26.24988884387752 30.11028804609415 21.38446185615904 38-39 21.89083974745329 26.658555210363843 30.2208795660465 21.22972547613637 40-41 22.165201287858363 26.528802492155553 30.011835480469696 21.29416073951639 42-43 21.905477825245324 26.59318902493899 29.796807145894768 21.704526003920925 44-45 22.077123293665302 26.812868138491353 29.592124077051306 21.51788449079204 46-47 22.294954006066654 27.10702605499511 29.052752225592588 21.545267713345652 48-49 22.454101343330564 27.223440038505892 28.776696877606877 21.54576174055667 50-51 22.418002069268262 27.389997783935083 28.615079404287876 21.576920742508783 52-53 22.675856987540982 27.503598460187217 28.263694880217766 21.556849672054035 54-55 22.367152554191254 27.552956188255422 28.350845421708687 21.729045835844637 56-57 22.460206108152438 27.78578415527699 28.200731724874835 21.553278011695742 58-59 22.61060210624973 27.331420271771428 28.33912991927595 21.71884770270289 60-61 22.532863395830127 27.4100764612972 28.699946221609313 21.357113921263355 62-63 22.275792807810713 27.395079206676993 28.897204229437527 21.43192375607477 64-65 22.21453343364438 27.396631863625903 28.65280191061495 21.736032792114763 66-67 22.38267912368042 27.558249336944908 28.310335190405144 21.74873634896953 68-69 22.309033781580688 27.45640915615918 28.4947837784048 21.73977328385533 70-71 22.424989025538384 27.4596203330308 28.35208048973623 21.763310151694583 72-73 22.48021065350012 27.40118736078999 28.2637248010479 21.854877184661987 74-75 22.284685297609048 27.88137842060912 27.91070246434888 21.923233817432948 76-77 22.448102441482476 27.612310028893532 28.09709187353468 21.84249565608931 78-79 22.4908357952356 27.60264121062074 28.031774418706412 21.87474857543725 80-81 22.413379386079445 27.909396821005473 27.897010853072075 21.780212939843015 82-83 22.446377662590816 27.855455852062583 27.867665403248658 21.83050108209794 84-85 22.406937558445225 27.84596926442825 27.76329022354747 21.983802953579055 86-87 22.395065091613816 27.839350820014598 27.87908472284368 21.88649936552791 88-89 22.401063993461904 27.688213781100778 27.805262942454302 22.10545928298302 90-91 22.44217143074935 27.65233618738341 27.806131631678614 22.099360750188627 92-93 22.540902182355467 27.579732039228265 27.825129253472312 22.054236524943953 94-95 22.383279013865227 27.568518045402513 27.818460526520088 22.229742414212176 96-97 22.311609780609572 27.603876278648286 27.821918716997217 22.262595223744924 98-99 22.180069065150327 27.732876098418895 27.614591618598894 22.47246321783188 100-101 22.231786375634066 27.778042468370355 27.76960766148685 22.22056349450873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 418.0 1 309.0 2 251.5 3 331.0 4 299.0 5 278.0 6 530.5 7 1229.0 8 2012.0 9 2423.5 10 2326.5 11 2167.5 12 2282.0 13 2473.5 14 2700.0 15 2768.0 16 2834.5 17 2992.0 18 3124.0 19 3235.0 20 3410.5 21 3743.5 22 4300.0 23 5117.0 24 5954.5 25 7184.0 26 9017.0 27 10900.0 28 12415.5 29 14150.5 30 16325.5 31 18468.5 32 21152.5 33 23974.0 34 27025.0 35 29885.5 36 33502.0 37 37161.0 38 39358.0 39 40812.5 40 41445.0 41 43149.0 42 45445.0 43 47642.5 44 50048.0 45 51566.0 46 52123.0 47 54562.5 48 58014.5 49 57256.5 50 54508.5 51 52204.0 52 49853.5 53 48836.0 54 48275.0 55 44179.5 56 38142.5 57 33475.5 58 29994.0 59 26330.5 60 21549.5 61 17349.0 62 13922.0 63 10622.0 64 7718.5 65 5596.5 66 4016.5 67 3216.5 68 2388.0 69 1539.5 70 1099.5 71 717.5 72 489.5 73 359.0 74 247.0 75 169.5 76 99.0 77 58.0 78 32.5 79 18.5 80 14.5 81 6.5 82 4.5 83 3.0 84 1.5 85 1.5 86 1.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04178058698901707 2 0.0 3 0.0 4 0.0 5 0.017926130228395835 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004975559768117742 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.528765792991306E-5 22-23 3.881642372290437E-4 24-25 5.29314868948696E-4 26-27 0.002434848397164001 28-29 0.003034738581972524 30-31 5.998901848085221E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011891940722380704 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.528765792991306E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.4115063171965224E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.7643828964956533E-4 84-85 7.057531585982612E-5 86-87 0.0 88-89 0.0 90-91 0.007692709428721047 92-93 0.0027877249764631322 94-95 0.0 96-97 0.0 98-99 2.1172594757947838E-4 100-101 0.012527118565119138 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1416926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.78722515931669 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5809171343952 45.409731516709016 2 12.469746227542378 14.411840658610627 3 3.852735331806085 6.679166522949988 4 1.6302036900475203 3.7681979076929997 5 0.8618047803439386 2.490065344255533 6 0.5376144656725529 1.8640348906035327 7 0.35590447228295974 1.4396712312516227 8 0.26255771664214345 1.2137985511132492 9 0.1950131720081142 1.0142343071878905 >10 1.1296420255633437 12.192481837545971 >50 0.08238175574341561 3.290921762655371 >100 0.039649293043313646 4.088288428992028 >500 0.001097960945397612 0.3583408616640777 >1k 4.879826423989387E-4 0.6813622718833978 >5k 2.4399132119946935E-4 1.0978639068846512 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8349 0.5892333121136883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7224 0.509836081771384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4848 0.3421491312884371 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1883 0.1328933197640526 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1725 0.12174241985820008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 3.528765792991307E-5 0.0 0.0 0.0 22-23 0.0 1.058629737897392E-4 0.0 1.058629737897392E-4 0.0 24-25 0.0 1.4115063171965227E-4 0.0 1.7643828964956533E-4 0.0 26-27 0.0 1.4115063171965227E-4 0.0 3.881642372290437E-4 0.0 28-29 0.0 1.4115063171965227E-4 0.0 0.0010233420799674788 0.0 30-31 0.0 1.4115063171965227E-4 0.0 0.002611286686813567 0.0 32-33 0.0 1.4115063171965227E-4 0.0 0.004481532557098959 0.0 34-35 0.0 1.4115063171965227E-4 0.0 0.006140052479804873 0.0 36-37 0.0 1.4115063171965227E-4 0.0 0.008010298350090267 0.0 38-39 0.0 1.4115063171965227E-4 0.0 0.013021145776137921 0.0 40-41 0.0 1.4115063171965227E-4 0.0 0.017326240043587314 0.0 42-43 0.0 1.4115063171965227E-4 0.0 0.021031444126228187 0.0 44-45 0.0 1.4115063171965227E-4 0.0 0.023431004865462277 0.0 46-47 0.0 1.4115063171965227E-4 0.0 0.02681862002673393 0.0 48-49 0.0 1.4115063171965227E-4 0.0 0.03130015258383289 0.0 50-51 0.0 1.4115063171965227E-4 0.0 0.036169849378160895 0.0 52-53 0.0 1.4115063171965227E-4 0.0 0.04852052965363046 0.0 54-55 0.0 1.4115063171965227E-4 0.0 0.05646025268786091 0.0 56-57 0.0 1.4115063171965227E-4 0.0 0.07551558797001395 0.0 58-59 0.0 1.4115063171965227E-4 0.0 0.10653343929040754 0.0 60-61 0.0 1.4115063171965227E-4 0.0 0.13458712734468842 0.0 62-63 0.0 1.4115063171965227E-4 0.0 0.14845517691114427 0.0 64-65 0.0 1.4115063171965227E-4 0.0 0.17280366088278426 0.0 66-67 0.0 1.4115063171965227E-4 0.0 0.22898161230720587 0.0 68-69 0.0 1.4115063171965227E-4 0.0 0.31300152583832885 0.0 70-71 0.0 1.4115063171965227E-4 0.0 0.3922928932068436 0.0 72-73 0.0 2.1172594757947838E-4 0.0 0.48280573579707053 0.0 74-75 0.0 2.1172594757947838E-4 0.0 0.5884216959813003 0.0 76-77 0.0 2.1172594757947838E-4 0.0 0.6601262168948837 0.0 78-79 0.0 2.1172594757947838E-4 0.0 0.7699061207148432 0.0 80-81 0.0 2.1172594757947838E-4 0.0 0.9266538972395171 0.0 82-83 0.0 2.1172594757947838E-4 0.0 1.0580298477125836 0.0 84-85 0.0 2.1172594757947838E-4 0.0 1.2238818399831748 0.0 86-87 0.0 2.1172594757947838E-4 0.0 1.4230100936816743 0.0 88-89 0.0 2.1172594757947838E-4 0.0 1.699524181220473 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5995 0.0 34.964012 1 GTATCAA 8680 0.0 31.10287 1 TAACGCA 125 7.3820665E-6 26.604832 4 ATCAACG 10945 0.0 24.611599 3 TCAACGC 10990 0.0 24.59728 4 TATCAAC 11055 0.0 24.40968 2 CAACGCA 11120 0.0 24.352444 5 AACGCAG 11390 0.0 23.85354 6 GTATAGG 430 0.0 23.212538 1 GTACATG 6770 0.0 23.028042 1 GCACCGT 235 4.652975E-9 22.233374 6 GAGTACT 6945 0.0 22.228336 12-13 TACATGG 6820 0.0 22.221777 2 ACATGGG 6675 0.0 21.565714 3 GTACTTT 7270 0.0 21.16855 14-15 ACGCAGA 12795 0.0 21.086472 7 AGTACTT 7320 0.0 20.992254 12-13 CGCAGAG 12785 0.0 20.954351 8 GTACCTG 915 0.0 20.778336 1 CCGTCCG 115 0.0037235871 20.652283 9 >>END_MODULE