FastQCFastQC Report
Thu 26 May 2016
SRR937723_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937723_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656190
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60030.914826498422713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48580.740334354379067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31480.4797390999558055No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11520.17555890824303935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.17494932870052882No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10120.15422362425517No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.14584190554564988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9300.14172724363370365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8270.1260305704140569No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7520.11460095399198403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG405.3466076E-447.5716131
TACCTGG4550.035.531722
GTATCAA63550.031.5896471
GTACCTG5950.029.5823481
GGTATCA42400.029.5078641
GTACATG52600.025.9563731
TATCAAC76800.025.756142
TCAACGC77100.025.6559224
CAACGCA78000.025.4818155
ATCAACG77850.025.4087563
AACGCAG79600.025.0660976
TACATGG53850.024.989032
ACATGGG52900.024.6288193
ACCTGGG7300.024.1005823
CATGGGG16950.023.2839724
GAGTACT43550.023.23318712-13
ACGCAGA87650.022.5471637
CGGTATA853.1470063E-622.35501734-35
CGCAGAG88550.022.2643538
ACTATAC3059.094947E-1221.8261363
Produced by FastQC (version 0.11.5)