##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937723_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 656190 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74827412792027 33.0 31.0 34.0 30.0 34.0 2 31.872803608710893 33.0 31.0 34.0 30.0 34.0 3 31.86389003185053 33.0 31.0 34.0 30.0 34.0 4 35.47451043142992 37.0 35.0 37.0 33.0 37.0 5 35.48103293253478 37.0 35.0 37.0 33.0 37.0 6 35.563826025998566 37.0 35.0 37.0 33.0 37.0 7 35.5138892698761 37.0 35.0 37.0 33.0 37.0 8 35.51899602249348 37.0 35.0 37.0 33.0 37.0 9 37.13602462701352 39.0 37.0 39.0 34.0 39.0 10-11 37.010221886953474 39.0 37.0 39.0 33.0 39.0 12-13 36.902240204818725 39.0 37.0 39.0 33.0 39.0 14-15 38.002464987275026 40.0 38.0 41.0 33.0 41.0 16-17 37.93329371066307 40.0 38.0 41.0 33.0 41.0 18-19 37.94629604230482 40.0 38.0 41.0 33.0 41.0 20-21 37.910449717307486 40.0 38.0 41.0 33.0 41.0 22-23 37.82723068013837 40.0 38.0 41.0 33.0 41.0 24-25 37.85180206952255 40.0 38.0 41.0 33.0 41.0 26-27 37.74542053368689 40.0 37.0 41.0 33.0 41.0 28-29 37.611403709291515 40.0 37.0 41.0 32.5 41.0 30-31 37.33345601121626 39.5 37.0 41.0 32.0 41.0 32-33 37.12215364452369 39.0 36.0 41.0 32.0 41.0 34-35 37.080472119355676 40.0 36.0 41.0 31.5 41.0 36-37 36.91516710099209 39.5 36.0 41.0 31.0 41.0 38-39 36.74521708651458 39.0 35.0 41.0 31.0 41.0 40-41 36.492887730687755 39.0 35.0 41.0 30.0 41.0 42-43 36.189896981057316 39.0 35.0 41.0 30.0 41.0 44-45 35.84061476096862 39.0 35.0 41.0 28.5 41.0 46-47 35.31843444733995 39.0 35.0 40.5 26.0 41.0 48-49 34.88044621222512 39.0 35.0 40.0 24.0 41.0 50-51 33.96714290068425 38.0 34.0 39.5 22.5 40.5 52-53 34.05559441625139 38.0 34.0 39.5 22.0 40.5 54-55 34.3559654977979 38.0 34.0 40.0 21.5 41.0 56-57 34.154424785504204 38.0 34.0 40.0 19.5 41.0 58-59 33.928173242505984 38.0 34.0 40.0 18.5 41.0 60-61 33.62124613297978 38.0 33.5 40.0 16.5 41.0 62-63 33.25789405507551 38.0 33.0 40.0 12.5 41.0 64-65 32.89063990612475 37.0 33.0 40.0 8.0 41.0 66-67 32.45927780063701 36.5 32.0 39.0 7.5 41.0 68-69 31.96152943507216 36.0 31.0 39.0 4.0 40.5 70-71 31.533209893475977 35.5 31.0 39.0 2.0 40.0 72-73 31.067097182218564 35.0 31.0 38.0 2.0 40.0 74-75 30.53150840457794 35.0 30.0 37.0 2.0 39.5 76-77 30.050027431079414 34.5 29.5 37.0 2.0 39.0 78-79 29.492376445846478 34.0 29.0 36.0 2.0 39.0 80-81 28.920878861305415 34.0 28.5 36.0 2.0 37.5 82-83 28.419495115743917 34.0 27.5 35.0 2.0 37.0 84-85 27.88609777655862 34.0 26.5 35.0 2.0 36.5 86-87 27.346225940657433 33.0 26.0 35.0 2.0 36.0 88-89 26.893753333638124 33.0 25.5 35.0 2.0 36.0 90-91 26.344745424343557 33.0 24.5 35.0 2.0 35.0 92-93 25.732959203889116 32.0 22.0 35.0 2.0 35.0 94-95 25.02093067556653 32.0 19.0 34.0 2.0 35.0 96-97 24.219490543897347 31.5 6.5 34.0 2.0 35.0 98-99 23.23363431323245 31.0 2.0 34.0 2.0 35.0 100-101 20.49753958457154 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.014775893986801236 1101 2 0.1480734981721632 1101 3 0.17070765877419447 1101 4 0.107191327923438 1101 5 0.13013443984861794 1101 6 0.12727773158696465 1101 7 0.07509950147186117 1101 8 0.08895245014800679 1101 9 0.1680838658602113 1101 10-11 0.14234399520590557 1101 12-13 0.10992903329064774 1101 14-15 0.23475441702383648 1101 16-17 0.41041325735411505 1101 18-19 0.2921574686862627 1101 20-21 0.280002869766669 1101 22-23 -0.02425466532334042 1101 24-25 0.12280781056254142 1101 26-27 -0.04960834752910159 1101 28-29 0.2686896014845175 1101 30-31 0.21306212891253296 1101 32-33 0.01279066412598695 1101 34-35 0.13612475016969938 1101 36-37 0.1722961631505413 1101 38-39 0.06305159791369164 1101 40-41 0.20545024753499774 1101 42-43 0.35670747239316114 1101 44-45 0.3022456095761896 1101 46-47 0.13131346411574896 1101 48-49 0.5372156556023029 1101 50-51 0.2998441429941394 1101 52-53 0.40297470682375547 1101 54-55 0.3682634082041787 1101 56-57 0.4879948546632633 1101 58-59 0.3983560432041031 1101 60-61 0.638898170938468 1101 62-63 0.6336475690521439 1101 64-65 0.33321431884078834 1101 66-67 0.4106579185948718 1101 68-69 0.34978880087929554 1101 70-71 0.41947547620604553 1101 72-73 0.33927757989454577 1101 74-75 0.3443465393171259 1101 76-77 0.4024829697750789 1101 78-79 0.6884365876652865 1101 80-81 0.6691702429576409 1101 82-83 0.6816343163351135 1101 84-85 0.6526496732040599 1101 86-87 0.7436094315002926 1101 88-89 0.6699614533950218 1101 90-91 0.6020992798652074 1101 92-93 0.691235007003737 1101 94-95 0.9814034958842477 1101 96-97 1.1414725196280457 1101 98-99 1.3079520212831106 1101 100-101 1.2318653896796476 1102 1 0.09073060517467013 1102 2 0.24577890157705085 1102 3 0.1967624330154969 1102 4 0.17370447319305526 1102 5 0.006719685345778714 1102 6 0.14194999081880866 1102 7 0.07944196384541158 1102 8 0.0763856272184924 1102 9 0.18396784754730078 1102 10-11 0.04685642769760534 1102 12-13 0.2245519463591492 1102 14-15 0.3043928193047236 1102 16-17 0.2820008330867694 1102 18-19 0.22733580023764688 1102 20-21 0.2767844033357676 1102 22-23 0.27553424275572524 1102 24-25 0.3084343697642353 1102 26-27 0.1114246321567336 1102 28-29 0.2511717135003124 1102 30-31 0.28609170919599336 1102 32-33 0.4531722744557811 1102 34-35 0.4823867782970126 1102 36-37 0.5911272073701852 1102 38-39 0.5117973829289042 1102 40-41 0.5132277511674204 1102 42-43 0.3765281813330432 1102 44-45 0.4206325383258118 1102 46-47 0.3500595232763217 1102 48-49 0.708666158659021 1102 50-51 0.5688766840474102 1102 52-53 0.6311678148096149 1102 54-55 0.5598663360765386 1102 56-57 0.6555552351770118 1102 58-59 0.8495362389438839 1102 60-61 0.5261750851583855 1102 62-63 0.6553434321987837 1102 64-65 0.711510231333591 1102 66-67 0.7245187075851902 1102 68-69 0.6180647007614226 1102 70-71 0.5576748895349937 1102 72-73 0.5949318277767759 1102 74-75 0.5429922612789149 1102 76-77 0.7002377193432991 1102 78-79 0.8827508130675952 1102 80-81 1.0269217027816389 1102 82-83 1.0003611853210508 1102 84-85 0.8279053137446759 1102 86-87 0.9100512989784306 1102 88-89 0.9441232539816902 1102 90-91 0.9166070518859328 1102 92-93 0.8553164830023157 1102 94-95 1.169541226509022 1102 96-97 1.2782600843948835 1102 98-99 1.576170350641334 1102 100-101 2.008990701519984 1103 1 0.22533623293098515 1103 2 0.20209381953580774 1103 3 0.2534235800876026 1103 4 0.24363383353337298 1103 5 0.1511022434269691 1103 6 0.08733276633856946 1103 7 0.18013943988535885 1103 8 0.37946823715041944 1103 9 0.35439397139808193 1103 10-11 0.3521557960106776 1103 12-13 0.42041894085628684 1103 14-15 0.33996688501577665 1103 16-17 0.28156205319593397 1103 18-19 0.2428369403963231 1103 20-21 0.3128446393959976 1103 22-23 0.3077162667185718 1103 24-25 0.32189493253986967 1103 26-27 0.3084959018307387 1103 28-29 0.31848567807454486 1103 30-31 0.24647663122475194 1103 32-33 0.24938549806626042 1103 34-35 0.3604388631984108 1103 36-37 0.2750741077554508 1103 38-39 0.3132642569985151 1103 40-41 0.3511613041694659 1103 42-43 0.36122217835580983 1103 44-45 0.48507018084564635 1103 46-47 0.5990395133522028 1103 48-49 0.5465060458139774 1103 50-51 0.481813164107173 1103 52-53 0.678858784418388 1103 54-55 0.5855993549602374 1103 56-57 0.3872315750725335 1103 58-59 0.7535638703687724 1103 60-61 0.8764970615077559 1103 62-63 0.692026992170014 1103 64-65 0.9144489972860583 1103 66-67 0.7658515502878984 1103 68-69 0.9111339856115208 1103 70-71 0.8453751142215182 1103 72-73 0.6173330186711148 1103 74-75 0.5377882314173483 1103 76-77 0.6194509262277421 1103 78-79 0.9110936523693383 1103 80-81 0.9475265165371383 1103 82-83 1.163359830511478 1103 84-85 1.037995578629463 1103 86-87 0.951333406013962 1103 88-89 0.9155529404798699 1103 90-91 1.0627608980397767 1103 92-93 0.9785327897802638 1103 94-95 1.0679494646432879 1103 96-97 1.162243224542408 1103 98-99 1.0168804269678517 1103 100-101 1.004685975297452 1104 1 0.18959292040469933 1104 2 0.0799236805648782 1104 3 0.08692899291299838 1104 4 -0.0169449205953498 1104 5 0.14713181432293254 1104 6 0.13917043355494485 1104 7 0.15153250409738916 1104 8 0.10493214298473674 1104 9 0.20613075356671118 1104 10-11 0.17027505773059204 1104 12-13 0.16765109932377698 1104 14-15 0.32673901239082426 1104 16-17 0.2978554609369155 1104 18-19 0.2911920652149931 1104 20-21 0.36851038620524434 1104 22-23 0.26890328444315514 1104 24-25 -0.026634555663434867 1104 26-27 0.11602282419887189 1104 28-29 0.0739882840747299 1104 30-31 0.16880459504789513 1104 32-33 0.03861607828523006 1104 34-35 0.12927979559361802 1104 36-37 0.1467669919337382 1104 38-39 0.35712267298513467 1104 40-41 0.5853847255665485 1104 42-43 0.2704031902304749 1104 44-45 0.17763362974394425 1104 46-47 0.48726620465340176 1104 48-49 0.11566369110497732 1104 50-51 0.08626285625420849 1104 52-53 0.2193929553890115 1104 54-55 0.3763479361904203 1104 56-57 0.3718879516218365 1104 58-59 0.16977789315414782 1104 60-61 0.3979115076795594 1104 62-63 0.45829382012674813 1104 64-65 0.5375441127486411 1104 66-67 0.5912737553940488 1104 68-69 0.5624296614940931 1104 70-71 0.6133200125567981 1104 72-73 0.6243004661140255 1104 74-75 0.5583361766228272 1104 76-77 0.3783019943806707 1104 78-79 0.39240333875151023 1104 80-81 0.4437769949732555 1104 82-83 0.7631574697541055 1104 84-85 0.7088161733550891 1104 86-87 0.7131998030237945 1104 88-89 0.7291571828497005 1104 90-91 1.0333110877603673 1104 92-93 0.6427574633805122 1104 94-95 0.6924562619801122 1104 96-97 0.9382376628702183 1104 98-99 0.9025731788378835 1104 100-101 0.9680755711429718 1105 1 0.13743539820299588 1105 2 0.02119906964427898 1105 3 0.13226663469616895 1105 4 0.0034158021690942064 1105 5 0.12422668814689786 1105 6 0.1092585809215123 1105 7 0.031192664125526903 1105 8 0.2192544414108326 1105 9 0.19722259389028096 1105 10-11 0.30449920703156863 1105 12-13 0.09595717511732715 1105 14-15 0.02191343866358153 1105 16-17 0.09640520594702906 1105 18-19 0.26343718523609283 1105 20-21 0.22852148705814557 1105 22-23 0.12027825147031734 1105 24-25 0.1361440647533385 1105 26-27 0.06572551917820846 1105 28-29 0.2931009852089943 1105 30-31 0.5032232840481612 1105 32-33 0.3486321250626858 1105 34-35 0.338234117232588 1105 36-37 0.2790979512232852 1105 38-39 0.5256220794741822 1105 40-41 0.6187230048244459 1105 42-43 0.5570662298857982 1105 44-45 0.5724338143918004 1105 46-47 0.5339200119162157 1105 48-49 0.21657154303499482 1105 50-51 0.38915951940376914 1105 52-53 0.053080746649015964 1105 54-55 0.45485349760781446 1105 56-57 0.4351051106827697 1105 58-59 0.46244473567590916 1105 60-61 0.6020737779688403 1105 62-63 0.5055768589027991 1105 64-65 0.4273599045362033 1105 66-67 0.5987503446844684 1105 68-69 0.5494878255668993 1105 70-71 0.63808260817801 1105 72-73 0.5517712302044124 1105 74-75 0.5712038236816497 1105 76-77 0.31727203306879304 1105 78-79 0.31542277073955205 1105 80-81 0.5102667074501746 1105 82-83 0.5927456391025636 1105 84-85 0.78946771648355 1105 86-87 0.6319272715896993 1105 88-89 0.6713310815571667 1105 90-91 0.7325384244349493 1105 92-93 0.5522333052867836 1105 94-95 0.7329496454321465 1105 96-97 0.9456620451791977 1105 98-99 1.438557527734396 1105 100-101 1.2144100914981983 1106 1 -0.06814015369608839 1106 2 0.08673794253609302 1106 3 -0.1554853192322554 1106 4 0.020738920227501012 1106 5 0.06253054115663303 1106 6 0.045202294071110316 1106 7 0.008379692783144321 1106 8 0.09672084755371202 1106 9 0.0018314654109161665 1106 10-11 -0.0740152931741136 1106 12-13 -0.16107353926633294 1106 14-15 0.06007417498607737 1106 16-17 -0.14500179391585988 1106 18-19 -0.19589282299097732 1106 20-21 -0.28435815780679974 1106 22-23 -0.04977008254859783 1106 24-25 -0.1499701886403244 1106 26-27 0.06326168399377963 1106 28-29 0.002353864682469009 1106 30-31 0.10244942555371495 1106 32-33 0.0477665690500757 1106 34-35 0.22524392115415282 1106 36-37 -0.1092538930109086 1106 38-39 0.16663143776295897 1106 40-41 0.4119142498539219 1106 42-43 0.5471020519069114 1106 44-45 0.4490226667159405 1106 46-47 0.5864712596880537 1106 48-49 0.2466688966617525 1106 50-51 0.3980216131923271 1106 52-53 0.49812308176487363 1106 54-55 0.3186963949065955 1106 56-57 0.27338925301103245 1106 58-59 0.3008959903036299 1106 60-61 0.5052737642570619 1106 62-63 0.49418237103771645 1106 64-65 0.4141520339753981 1106 66-67 0.22916221222869382 1106 68-69 0.04665462935134457 1106 70-71 -0.24593631407621075 1106 72-73 0.26957940242435185 1106 74-75 0.41917533746199 1106 76-77 0.22937524848082802 1106 78-79 0.517578947895732 1106 80-81 0.29181236767230345 1106 82-83 0.13822332318318686 1106 84-85 0.11028219612155965 1106 86-87 0.6035318924590243 1106 88-89 0.7679142777727144 1106 90-91 0.5823413176201981 1106 92-93 0.4971265248123551 1106 94-95 0.7249376819054802 1106 96-97 0.6020363081711082 1106 98-99 0.799321273792259 1106 100-101 0.7896174821467632 1107 1 0.08509799982901001 1107 2 0.08414741971601458 1107 3 0.14679317702041672 1107 4 0.028075056114573727 1107 5 0.002155908615577573 1107 6 0.056682062509274544 1107 7 0.08397841257124838 1107 8 6.68836437135667E-4 1107 9 0.09999040833313444 1107 10-11 0.1821444724548087 1107 12-13 0.03384574517949801 1107 14-15 0.045272477401020694 1107 16-17 0.07370374731479501 1107 18-19 -0.03673318779116386 1107 20-21 -0.07000493570608768 1107 22-23 -0.011446999605716712 1107 24-25 0.07673768024832128 1107 26-27 0.19925911554357612 1107 28-29 0.029336638006704163 1107 30-31 0.02765836295777291 1107 32-33 0.16394178551223604 1107 34-35 0.37431135300494134 1107 36-37 0.3593227240592469 1107 38-39 0.25253205077978436 1107 40-41 0.3154739613045905 1107 42-43 0.10403837240134095 1107 44-45 0.26011315442048755 1107 46-47 0.2611288832696417 1107 48-49 0.3215452398307761 1107 50-51 0.3115501219862651 1107 52-53 0.2817542282367711 1107 54-55 0.41197628574746403 1107 56-57 0.2669289987745884 1107 58-59 0.3327818949298518 1107 60-61 0.37661347189519034 1107 62-63 0.3434534729630556 1107 64-65 0.030002180958973668 1107 66-67 0.2497098138379883 1107 68-69 0.27142111107622213 1107 70-71 0.2273939424678204 1107 72-73 0.2964508093531464 1107 74-75 0.1264853875267029 1107 76-77 0.22510620332225884 1107 78-79 0.0633304704047859 1107 80-81 0.2736275395879062 1107 82-83 0.4486907610537827 1107 84-85 0.4451348613902937 1107 86-87 0.568650131465354 1107 88-89 0.24687806864468342 1107 90-91 0.4662483718285415 1107 92-93 0.5062475329290592 1107 94-95 0.35742657034845493 1107 96-97 0.6565958089286035 1107 98-99 0.6206474368687793 1107 100-101 0.06965279833703164 1108 1 -0.1472220860268294 1108 2 -0.10463993473718958 1108 3 -0.27876394235239843 1108 4 -0.13531706815812328 1108 5 -0.263478790240967 1108 6 -0.21574879920962076 1108 7 -0.10060482507617508 1108 8 -0.11631429479997024 1108 9 -0.1799778504712961 1108 10-11 0.01161967407733755 1108 12-13 0.09039100253101395 1108 14-15 -0.19504202418716687 1108 16-17 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100-101 -0.10098980530262835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2781.0 3 486.0 4 399.0 5 465.0 6 504.0 7 654.0 8 808.0 9 1012.0 10 1290.0 11 1837.0 12 2769.0 13 3825.0 14 4182.0 15 5839.0 16 7500.0 17 11048.0 18 9182.0 19 5046.0 20 4760.0 21 4778.0 22 5142.0 23 5568.0 24 6300.0 25 7221.0 26 8136.0 27 9607.0 28 11238.0 29 13460.0 30 16007.0 31 20007.0 32 25298.0 33 32875.0 34 44417.0 35 62749.0 36 87935.0 37 123169.0 38 96837.0 39 11055.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90284564969677 20.405472579744725 14.903220379165042 20.788461391393458 2 14.459074586205473 21.24703231319816 42.40478560039381 21.88910750020256 3 18.56867897933569 28.196226092034678 30.742994819017216 22.492100109612412 4 11.232394204706498 17.48555686520913 39.50210742114943 31.779941508934943 5 11.332879738851895 39.89159601550376 34.768322796180634 14.007201449463713 6 27.801967375351456 38.32609681710373 19.10852636224936 14.76340944529545 7 26.174040532078983 35.33071671833718 21.705279555277265 16.78996319430657 8 25.657464706133894 35.33952974092378 20.535654288105462 18.46735126483686 9 27.239961636438053 16.84316145072909 21.88540413080264 34.031472782030214 10-11 23.678165659416607 28.270202656574313 27.63057213941746 20.421059544591625 12-13 25.00551666950454 26.371970890310287 28.64086825124249 19.981644188942685 14-15 22.440609995968384 26.85736930839432 28.29248775243424 22.409532943203057 16-17 19.39202753958854 30.524316683886543 29.090141294644734 20.993514481880183 18-19 19.15601149956285 28.373224191842638 33.16295247076431 19.3078118378302 20-21 20.415472724676786 27.19683417357836 33.146730984922925 19.240962116821926 22-23 19.911692936351113 27.324111203071126 32.906177371524905 19.858018489052856 24-25 19.472353655752485 27.67948153130901 33.32488358047588 19.523281232462626 26-27 19.372357336240743 27.67404360194784 33.333715083244385 19.61988397856703 28-29 18.934891360873728 27.59074133326817 33.84196792868221 19.632399377175894 30-31 19.312164463485225 27.70174192612278 33.59290399932797 19.393189611064017 32-33 18.99733246171857 27.789517254230223 33.38699466263239 19.826155621418813 34-35 19.34480512389335 27.768957435548497 33.29148443686888 19.59475300368927 36-37 19.622716990408744 27.530559099248077 33.07643153089807 19.770292379445106 38-39 19.33325496039622 28.045551979578253 33.14595076551021 19.47524229451532 40-41 19.260563299638367 27.8476267836435 33.406494748791694 19.485315167926444 42-43 19.03781312418684 27.990523049482935 32.893157537886424 20.0785062884438 44-45 19.30073731986976 28.23844997892499 32.49201287713576 19.968799824069492 46-47 19.552257838885822 28.322921701627912 31.770786801700986 20.35403365778528 48-49 19.556507496535215 28.84320081245872 31.396871575780484 20.203420115225583 50-51 19.847413932661144 28.782732468103696 31.195611336972224 20.174242262262933 52-53 19.668082629489557 29.168273979257837 31.02746041045862 20.136182980793983 54-55 19.579364006807502 29.0122933640572 31.0669137403024 20.341428888832898 56-57 19.78428744167868 29.53318513813909 30.46937977868432 20.213147641497912 58-59 19.833225702539018 28.90589099214219 30.917518171284105 20.343365134034688 60-61 19.935975525980748 29.130525049537297 31.000424387309906 19.933075037172053 62-63 19.912075261987933 28.984781493514422 31.213892688765664 19.889250555731977 64-65 19.753551014924646 28.775434182564304 31.23977136715601 20.23124343535504 66-67 19.72029989786618 29.25080493356722 30.651519106962656 20.377376061603943 68-69 19.521488072699736 29.198220371071564 30.864179624042702 20.416111932186002 70-71 19.739041753624758 29.18013340274714 30.784386168739665 20.296438674888435 72-73 19.733462386045208 29.19540264980432 30.395959265956723 20.675175698193744 74-75 19.48805914091771 29.485036138618398 30.472779238386632 20.55412548207726 76-77 19.646344424069103 29.045502503317145 30.78331915881215 20.524833913801608 78-79 19.559451589605306 28.907362985199512 30.72806839402606 20.80511703116912 80-81 19.470286437082393 29.578142139907833 30.218375546091536 20.733195876918238 82-83 19.803732082690225 29.444385922326394 30.158788317356795 20.593093677626587 84-85 19.569312545701294 29.12838189564763 30.107210080159497 21.19509547849158 86-87 19.533898208058204 29.27123222426865 30.40561221179673 20.78925735587642 88-89 19.895926603599744 28.71842551011918 30.477994132990137 20.907653753290937 90-91 19.518218530761185 28.72122937866909 30.940889971219836 20.81966211934989 92-93 19.622284626898825 29.00448596161934 30.717730959852286 20.655498451629548 94-95 19.241741036838903 28.940054244233405 30.55585453751562 21.262350181412078 96-97 19.4718935329057 28.807382589784282 30.197610536175162 21.523113341134852 98-99 19.261825457837848 28.788684998247966 30.239933063950343 21.709556479963847 100-101 19.559092206462132 28.290858417115533 30.900864049232947 21.24918532718939 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1347.0 1 833.5 2 425.5 3 528.5 4 415.5 5 385.0 6 670.0 7 1237.0 8 1932.5 9 2275.0 10 1968.0 11 1609.0 12 1663.5 13 1734.0 14 1775.5 15 1862.0 16 1907.0 17 1982.0 18 2122.5 19 2272.5 20 2451.5 21 2746.5 22 3121.0 23 3543.5 24 4002.5 25 4823.5 26 6189.5 27 7529.5 28 8103.0 29 8871.0 30 10470.5 31 12185.0 32 14451.0 33 17362.5 34 20320.5 35 22491.5 36 25413.5 37 28074.0 38 27627.0 39 26343.0 40 25220.0 41 24061.0 42 22626.0 43 22242.0 44 23113.0 45 23211.0 46 21921.5 47 21230.0 48 23850.0 49 23276.5 50 19732.0 51 17350.5 52 14783.0 53 13405.5 54 12478.0 55 11127.5 56 9713.0 57 8421.5 58 7264.5 59 6288.5 60 5280.0 61 4173.5 62 3178.5 63 2309.5 64 1593.0 65 1114.0 66 779.5 67 567.0 68 414.5 69 311.0 70 211.0 71 135.0 72 103.5 73 87.0 74 57.5 75 49.0 76 44.5 77 26.5 78 12.5 79 7.0 80 6.0 81 5.5 82 3.0 83 1.5 84 2.0 85 2.0 86 2.0 87 1.5 88 1.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36346180222191743 2 0.3140858592785626 3 0.31500022859232846 4 0.3159145979060943 5 0.3284109785275606 6 0.21472439384934242 7 0.21335283987869366 8 0.21365762964994894 9 0.21365762964994894 10-11 0.21899145064691627 12-13 0.20672366235389142 14-15 0.20817141376735399 16-17 0.2169341196909432 18-19 0.21068592938020997 20-21 0.20138984135692406 22-23 0.20055166948597208 24-25 0.2039805544125939 26-27 0.19978969505783387 28-29 0.21800088389033662 30-31 0.22173455858821378 32-33 0.22348709977293163 34-35 0.22150596625977234 36-37 0.1936177021899145 38-39 0.18317865252442128 40-41 0.18980783004922355 42-43 0.2039043569697801 44-45 0.21121931147990675 46-47 0.21236227312211403 48-49 0.2114479038083482 50-51 0.21518157850622532 52-53 0.24832746613023668 54-55 0.20184702601380697 56-57 0.18256907298191072 58-59 0.17875920084121977 60-61 0.17190143098797603 62-63 0.18256907298191072 64-65 0.1776924366418263 66-67 0.17784483152745392 68-69 0.18622655023697404 70-71 0.17997835992624087 72-73 0.1768542647708743 74-75 0.18470260138069766 76-77 0.19186516100519665 78-79 0.19491305871774942 80-81 0.182035690882214 82-83 0.17784483152745392 84-85 0.17083466678858258 86-87 0.17045367957451346 88-89 0.1785306085127783 90-91 0.18668373489385695 92-93 0.17472073637208735 94-95 0.1812737164540758 96-97 0.1812737164540758 98-99 0.1884362760785748 100-101 0.15529038845456347 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 656190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.664955986116276 #Duplication Level Percentage of deduplicated Percentage of total 1 84.8955332305463 49.8039272038787 2 8.992272649807557 10.550625584322349 3 2.3703816119463728 4.171749988054 4 1.0077954046388635 2.3648909222459666 5 0.5411012518726398 1.5871840562570412 6 0.3522892169458912 1.2400218843908444 7 0.23366514736422334 0.9595568909928074 8 0.17863316313093927 0.8383605322189824 9 0.14547906884601905 0.7681070853677596 >10 1.097380760616418 12.999016391764384 >50 0.11845049703143617 4.784525591216064 >100 0.0641606407813838 6.670932776823197 >500 0.0012987983963821607 0.6215902502886412 >1k 0.0012987983963821607 1.7247262693716212 >5k 2.5975967927643217E-4 0.9147845728077258 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6003 0.914826498422713 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4858 0.740334354379067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3148 0.4797390999558055 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1152 0.17555890824303935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1148 0.17494932870052882 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1012 0.15422362425517 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 957 0.14584190554564988 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 930 0.14172724363370365 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 827 0.1260305704140569 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 752 0.11460095399198403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.5239488562763834E-4 0.0 0.0 0.0 0.0 7 3.047897712552767E-4 0.0 0.0 0.0 0.0 8 3.047897712552767E-4 0.0 0.0 0.0 0.0 9 3.047897712552767E-4 0.0 0.0 0.0 0.0 10-11 3.047897712552767E-4 0.0 0.0 0.0 0.0 12-13 3.047897712552767E-4 0.0 0.0 0.0 0.0 14-15 3.047897712552767E-4 0.0 0.0 0.0 0.0 16-17 3.047897712552767E-4 0.0 0.0 0.0 0.0 18-19 3.047897712552767E-4 0.0 0.0 0.0 0.0 20-21 3.047897712552767E-4 0.0 0.0 0.0 0.0 22-23 3.047897712552767E-4 0.0 0.0 1.5239488562763834E-4 0.0 24-25 3.047897712552767E-4 0.0 0.0 5.333820996967342E-4 0.0 26-27 3.047897712552767E-4 0.0 0.0 9.905667565796492E-4 0.0 28-29 3.047897712552767E-4 0.0 0.0 0.0025907130556698517 0.0 30-31 3.047897712552767E-4 0.0 0.0 0.004495649126015331 0.0 32-33 3.047897712552767E-4 0.0 0.0 0.007162559624499002 0.0 34-35 3.047897712552767E-4 0.0 0.0 0.010286654779865588 0.0 36-37 3.047897712552767E-4 0.0 0.0 0.014020329477742727 0.0 38-39 3.047897712552767E-4 0.0 0.0 0.021106691659427912 0.0 40-41 3.047897712552767E-4 0.0 0.0 0.0274310794129749 0.0 42-43 3.047897712552767E-4 0.0 0.0 0.038784498392233954 0.0 44-45 3.047897712552767E-4 0.0 0.0 0.047775796644264616 0.0 46-47 3.047897712552767E-4 0.0 0.0 0.06766332921867142 0.0 48-49 3.047897712552767E-4 0.0 0.0 0.09166552370502445 0.0 50-51 3.047897712552767E-4 0.0 0.0 0.10355232478398024 0.0 52-53 3.047897712552767E-4 0.0 0.0 0.12252548804462123 0.0 54-55 3.047897712552767E-4 0.0 0.0 0.15483320379768056 0.0 56-57 3.047897712552767E-4 0.0 0.0 0.20489492372635976 0.0 58-59 3.047897712552767E-4 0.0 0.0 0.2596808851094957 0.0 60-61 3.047897712552767E-4 0.0 0.0 0.33008732226946463 0.0 62-63 3.047897712552767E-4 0.0 0.0 0.39973178500129536 0.0 64-65 3.047897712552767E-4 0.0 0.0 0.47608162270074217 0.0 66-67 3.047897712552767E-4 0.0 0.0 0.5679757387342081 0.0 68-69 3.047897712552767E-4 0.0 0.0 0.6783096359286183 0.0 70-71 3.047897712552767E-4 0.0 0.0 0.7605266767247292 0.0 72-73 3.047897712552767E-4 0.0 0.0 0.8685746506347247 0.0 74-75 3.047897712552767E-4 0.0 0.0 0.9907191514652769 0.0 76-77 3.047897712552767E-4 0.0 0.0 1.114997180694616 0.0 78-79 3.047897712552767E-4 0.0 0.0 1.2609152836830795 0.0 80-81 3.047897712552767E-4 0.0 0.0 1.4718298053917311 0.0 82-83 3.047897712552767E-4 0.0 0.0 1.7062131394870388 0.0 84-85 3.047897712552767E-4 0.0 0.0 1.9807525259452294 0.0 86-87 3.047897712552767E-4 0.0 0.0 2.3354516222435575 0.0 88-89 3.047897712552767E-4 0.0 0.0 2.7328212865176242 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGGG 40 5.3466076E-4 47.571613 1 TACCTGG 455 0.0 35.53172 2 GTATCAA 6355 0.0 31.589647 1 GTACCTG 595 0.0 29.582348 1 GGTATCA 4240 0.0 29.507864 1 GTACATG 5260 0.0 25.956373 1 TATCAAC 7680 0.0 25.75614 2 TCAACGC 7710 0.0 25.655922 4 CAACGCA 7800 0.0 25.481815 5 ATCAACG 7785 0.0 25.408756 3 AACGCAG 7960 0.0 25.066097 6 TACATGG 5385 0.0 24.98903 2 ACATGGG 5290 0.0 24.628819 3 ACCTGGG 730 0.0 24.100582 3 CATGGGG 1695 0.0 23.283972 4 GAGTACT 4355 0.0 23.233187 12-13 ACGCAGA 8765 0.0 22.547163 7 CGGTATA 85 3.1470063E-6 22.355017 34-35 CGCAGAG 8855 0.0 22.264353 8 ACTATAC 305 9.094947E-12 21.826136 3 >>END_MODULE