FastQCFastQC Report
Thu 26 May 2016
SRR937723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656190
Sequences flagged as poor quality0
Sequence length101
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66821.0183026257638794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59970.913912129108947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.6394489400935705No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.217010317133757No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13020.1984181410871851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.19735137688779164No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12940.19719898200216399No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8650.13182157606790715No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.11764885170453679No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7520.11460095399198403No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6580.10027583474298604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG601.4886791E-647.512122
TATCACG554.9535083E-543.1928372
TACCTGG4900.041.694312
GGACCGA704.308835E-640.712266
ATCACGC608.274411E-539.5934333
CGTGCAA1106.998562E-834.5490049
GTACCTG6300.033.188131
AGGACCG1252.1467167E-730.4077575
GACCGAG953.48614E-530.003087
ACCGTGC1153.8581584E-628.9160127
ACCTGGG7650.027.9483073
TAACGCA1056.8894165E-527.1497824
GGTATCA57200.026.7504121
CCGTGCA1257.3786887E-626.6027328
TCACGCA908.930126E-426.3956224
GTATAGG3250.026.3184171
TGACCGT1309.99272E-625.5834485
GTATCAA77800.025.4088121
CTGACCG1601.853412E-623.7560624
GTACATG54200.023.2336391
Produced by FastQC (version 0.11.5)