##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937723_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 656190 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13929654520794 33.0 31.0 34.0 31.0 34.0 2 32.41310443621512 34.0 31.0 34.0 31.0 34.0 3 32.48004846157363 34.0 31.0 34.0 31.0 34.0 4 36.122356329721576 37.0 35.0 37.0 35.0 37.0 5 36.045116505890064 37.0 35.0 37.0 35.0 37.0 6 36.110160167024794 37.0 35.0 37.0 35.0 37.0 7 36.061805269815146 37.0 35.0 37.0 35.0 37.0 8 36.04465779728432 37.0 35.0 37.0 35.0 37.0 9 37.72657919200232 39.0 37.0 39.0 35.0 39.0 10-11 37.653243725140584 39.0 37.0 39.0 35.0 39.0 12-13 37.54090431125131 39.0 37.0 39.0 35.0 39.0 14-15 38.68221094500069 40.0 38.0 41.0 34.0 41.0 16-17 38.63522302991512 40.0 38.0 41.0 34.5 41.0 18-19 38.71324692543318 40.0 38.0 41.0 35.0 41.0 20-21 38.65674423566345 40.0 38.0 41.0 34.0 41.0 22-23 38.59809811182737 40.0 38.0 41.0 34.0 41.0 24-25 38.606991115378165 40.0 38.0 41.0 34.0 41.0 26-27 38.447859613831355 40.0 38.0 41.0 34.0 41.0 28-29 38.282764900409944 40.0 38.0 41.0 34.0 41.0 30-31 38.250210304942165 40.0 37.5 41.0 34.0 41.0 32-33 38.087775644249376 40.0 37.0 41.0 34.0 41.0 34-35 37.92180847010775 40.0 37.0 41.0 33.5 41.0 36-37 37.74907039119768 40.0 37.0 41.0 33.0 41.0 38-39 37.598437952422316 40.0 36.5 41.0 33.0 41.0 40-41 37.37693960590683 40.0 36.0 41.0 33.0 41.0 42-43 37.09743214617717 40.0 36.0 41.0 32.5 41.0 44-45 36.68446486535912 40.0 36.0 41.0 31.0 41.0 46-47 36.12034852710343 39.5 35.5 41.0 30.0 41.0 48-49 35.6336465048233 39.0 35.0 41.0 27.5 41.0 50-51 35.32426583763849 39.0 35.0 41.0 26.5 41.0 52-53 35.07884225605389 39.0 35.0 40.0 25.5 41.0 54-55 34.8670575595483 39.0 35.0 40.0 24.5 41.0 56-57 34.629948642923544 38.5 35.0 40.0 23.5 41.0 58-59 34.38950989804782 38.0 34.0 40.0 23.0 41.0 60-61 34.082121032018165 38.0 34.0 40.0 22.5 41.0 62-63 33.70183788232067 38.0 34.0 40.0 21.5 41.0 64-65 33.303064661149975 37.0 33.0 40.0 19.5 41.0 66-67 32.88569621603499 36.5 32.5 39.0 18.0 40.5 68-69 32.45201542236242 36.0 32.0 39.0 13.5 40.0 70-71 32.06488898032582 35.5 32.0 38.5 11.5 40.0 72-73 31.560145689510662 35.0 31.0 38.0 7.5 40.0 74-75 31.07642070132126 35.0 31.0 37.0 7.0 39.0 76-77 29.83666163763544 33.5 29.5 36.0 3.5 38.5 78-79 30.40048004388973 34.0 30.5 36.0 2.0 39.0 80-81 30.198709977293163 34.0 30.5 36.0 2.0 38.0 82-83 29.82280894253189 34.0 30.0 35.5 2.0 37.0 84-85 29.414895838095674 34.0 30.0 35.0 2.0 37.0 86-87 29.04908791660952 34.0 29.0 35.0 2.0 36.0 88-89 28.593728188481993 34.0 29.0 35.0 2.0 36.0 90-91 28.035021868666085 34.0 28.5 35.0 2.0 35.5 92-93 27.60031012359225 33.0 27.0 35.0 2.0 35.0 94-95 27.1276131913013 33.0 26.0 35.0 2.0 35.0 96-97 26.541952026090005 33.0 25.0 35.0 2.0 35.0 98-99 25.666503604139045 32.0 24.0 34.0 2.0 35.0 100-101 22.927532422011918 29.5 9.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18968460087612726 1101 2 0.1788944732289437 1101 3 0.14094071064741343 1101 4 0.03188389502000177 1101 5 -4.559365570742102E-4 1101 6 -0.010763018017733827 1101 7 0.02304119266742788 1101 8 -0.05075194996882004 1101 9 0.024466176773259463 1101 10-11 0.07578913900439943 1101 12-13 0.024867919543858363 1101 14-15 0.07330480617724788 1101 16-17 0.06722363018112532 1101 18-19 -0.06119533611661154 1101 20-21 0.11099106358447841 1101 22-23 -0.009383296686159781 1101 24-25 -0.03268336250149417 1101 26-27 -0.09039718410683406 1101 28-29 -0.33265569979339915 1101 30-31 -0.10738577651513737 1101 32-33 -0.010616614956376225 1101 34-35 0.01953957224964853 1101 36-37 -0.22862276907512324 1101 38-39 0.06773646780660414 1101 40-41 -0.07816262822798592 1101 42-43 -0.31569134821248923 1101 44-45 -0.15163237240092542 1101 46-47 -0.27876340507295083 1101 48-49 -0.29798138935750984 1101 50-51 0.034175695843678966 1101 52-53 -0.016944963303430427 1101 54-55 -0.10292185784692975 1101 56-57 -0.026629112481799666 1101 58-59 -0.31089171272798666 1101 60-61 -0.15534149132217578 1101 62-63 -0.1794809281143941 1101 64-65 -0.1699722990297161 1101 66-67 -0.23884738030537278 1101 68-69 -0.11875403233464255 1101 70-71 -0.23367217903415494 1101 72-73 -0.1926711152798788 1101 74-75 -0.2683620313825088 1101 76-77 -0.29308929881540635 1101 78-79 -0.1417485180789413 1101 80-81 -0.031458853147714905 1101 82-83 -0.18626841548923778 1101 84-85 -0.24059220204917153 1101 86-87 -0.4605077663570718 1101 88-89 -0.3819682137731846 1101 90-91 -0.44690261152169697 1101 92-93 -0.7566951649985185 1101 94-95 -0.8736556566584781 1101 96-97 -0.5762114642563141 1101 98-99 -0.5512105798299736 1101 100-101 -0.9170475119110897 1102 1 0.1795302570430266 1102 2 0.10533030950934119 1102 3 0.04244028572212244 1102 4 -0.0018853294227980655 1102 5 0.028948893884027882 1102 6 0.07710056725517944 1102 7 0.07040802656275957 1102 8 0.07305757384070688 1102 9 0.117549489545695 1102 10-11 0.05399458353108599 1102 12-13 0.14355640818053672 1102 14-15 0.24079390889950503 1102 16-17 0.19534546037549205 1102 18-19 0.18523940358237923 1102 20-21 0.17901474974288334 1102 22-23 0.11668518482274237 1102 24-25 0.0057216598413489805 1102 26-27 0.03359327563492087 1102 28-29 -0.10818000277884465 1102 30-31 0.06099704005421103 1102 32-33 0.2796347763312852 1102 34-35 0.1997873996477395 1102 36-37 0.06482241556177115 1102 38-39 0.023821123891266893 1102 40-41 0.04192948461024315 1102 42-43 -0.1478897197890987 1102 44-45 -0.16791660630174476 1102 46-47 0.01748902796704499 1102 48-49 0.017910816690140052 1102 50-51 0.034266163809796524 1102 52-53 0.2373248505518717 1102 54-55 0.12620608578878745 1102 56-57 0.14000465674264717 1102 58-59 0.08388581831897568 1102 60-61 0.11991140776492415 1102 62-63 0.1368334617751259 1102 64-65 0.2091241178905321 1102 66-67 0.19559624170301504 1102 68-69 0.1420349579880238 1102 70-71 -0.017272804085557425 1102 72-73 -0.027410814268286288 1102 74-75 0.02616328594073636 1102 76-77 0.05011600863860721 1102 78-79 0.14221672029044186 1102 80-81 0.014189089391422272 1102 82-83 0.11584250372012761 1102 84-85 0.09518650782280247 1102 86-87 0.1329319353727847 1102 88-89 0.27087330279890054 1102 90-91 0.19973499573767484 1102 92-93 0.3060484816829536 1102 94-95 0.19248190947908483 1102 96-97 -0.00225801413705895 1102 98-99 0.09172606517920556 1102 100-101 0.09523968275952299 1103 1 0.31731717839159046 1103 2 0.15485389601923316 1103 3 0.23844722145508257 1103 4 0.12405509240803525 1103 5 0.07186234775638667 1103 6 0.10686066002897121 1103 7 0.01293510881587423 1103 8 0.12627650103223687 1103 9 0.22148512772790951 1103 10-11 0.20595069439131208 1103 12-13 0.26135737940616366 1103 14-15 0.18534038221309856 1103 16-17 0.3036410207258484 1103 18-19 0.13248713302188975 1103 20-21 -0.0047877491281127504 1103 22-23 0.009802975200805975 1103 24-25 -0.006936052816364224 1103 26-27 0.10953620637237549 1103 28-29 0.29674748981988586 1103 30-31 0.24466628810701962 1103 32-33 0.32513686429912525 1103 34-35 0.4386832577216708 1103 36-37 0.507356745767332 1103 38-39 0.33180390721651776 1103 40-41 0.5220372003343954 1103 42-43 0.5718302862322844 1103 44-45 0.5921917784641764 1103 46-47 0.4179350558103323 1103 48-49 0.452297333268433 1103 50-51 0.4713394406090927 1103 52-53 0.6867726123284044 1103 54-55 0.6366152605815287 1103 56-57 0.6829337215895137 1103 58-59 0.6817866997815045 1103 60-61 0.7074521774974727 1103 62-63 0.8428938650958031 1103 64-65 1.002311042584303 1103 66-67 1.1213627126749657 1103 68-69 1.035046259218511 1103 70-71 0.7041549310408044 1103 72-73 0.7062085344195594 1103 74-75 0.6084147229866943 1103 76-77 0.5704125056474361 1103 78-79 0.5748771091152172 1103 80-81 0.8749793392227652 1103 82-83 0.8134110944394024 1103 84-85 0.6485002159310334 1103 86-87 0.6855832921337317 1103 88-89 0.7769866719259682 1103 90-91 0.7453608564320255 1103 92-93 0.7602007221365632 1103 94-95 0.697050349663968 1103 96-97 0.7659474485889533 1103 98-99 0.5891056515998905 1103 100-101 0.41044365686761 1104 1 0.23494819900805197 1104 2 0.14515995337485776 1104 3 0.09777637132726369 1104 4 0.05012548985259713 1104 5 -0.044318479831780166 1104 6 -0.0383263907726743 1104 7 0.08966681129239618 1104 8 -0.05075194996882004 1104 9 0.0038581236121757456 1104 10-11 0.1706043066430496 1104 12-13 0.06717315433091642 1104 14-15 0.09509492676554743 1104 16-17 0.33241334183799154 1104 18-19 0.15574976019139086 1104 20-21 0.09765664596324797 1104 22-23 0.050921310762902294 1104 24-25 0.055930452875252 1104 26-27 0.04993251686205724 1104 28-29 0.04775102434052769 1104 30-31 -0.06414650515660725 1104 32-33 -6.674990382578017E-4 1104 34-35 0.09083421199499497 1104 36-37 0.10429853485852192 1104 38-39 0.07199655749271017 1104 40-41 -0.025048592121201807 1104 42-43 0.116892471114177 1104 44-45 0.08315642380716781 1104 46-47 -0.17119759267025358 1104 48-49 -0.25572669013122606 1104 50-51 -0.23814824290955272 1104 52-53 -0.06785286079175279 1104 54-55 -0.1648674188497381 1104 56-57 0.05474591946597229 1104 58-59 -0.24013902330676018 1104 60-61 -0.038731556147098445 1104 62-63 -0.21244347371257533 1104 64-65 -0.07252463528288189 1104 66-67 -0.14781076235421864 1104 68-69 0.019616399134491758 1104 70-71 0.07699547123652906 1104 72-73 -0.27610823045448996 1104 74-75 -0.4322169071928208 1104 76-77 -0.31223065897711777 1104 78-79 -0.19565311623410153 1104 80-81 -0.14056629058817194 1104 82-83 -0.30585239745908055 1104 84-85 -0.3217192031412033 1104 86-87 -0.2488520587430436 1104 88-89 -0.33702583592983615 1104 90-91 -0.3471663258273274 1104 92-93 -0.19633247069799964 1104 94-95 -0.15527000643426447 1104 96-97 -0.0022384397901298314 1104 98-99 0.09054863855334716 1104 100-101 0.05731527532838854 1105 1 0.15869303326462614 1105 2 0.11828160113710595 1105 3 0.16518382758330574 1105 4 0.08581308327561743 1105 5 0.04349915843429386 1105 6 0.1376825778371895 1105 7 -9.072849481839285E-5 1105 8 -0.006634302909994005 1105 9 0.010348275726833833 1105 10-11 0.17727306158897704 1105 12-13 0.23215545891194012 1105 14-15 0.22537607509931945 1105 16-17 0.213222055457976 1105 18-19 0.07968968369441853 1105 20-21 0.06758267691375153 1105 22-23 0.097116246136153 1105 24-25 0.03760580238725453 1105 26-27 -0.15605425004201123 1105 28-29 -0.07160787392729873 1105 30-31 -1.1601627944202164E-4 1105 32-33 -0.054284068984614464 1105 34-35 0.10086894337633368 1105 36-37 0.16087166859632163 1105 38-39 0.3016825462379771 1105 40-41 0.26672523624421984 1105 42-43 0.33484837544899904 1105 44-45 0.42882709117724715 1105 46-47 0.44487776123813205 1105 48-49 0.5467001101853128 1105 50-51 0.5705873589545192 1105 52-53 0.7125563320333583 1105 54-55 0.7557092759537412 1105 56-57 0.6619350955848446 1105 58-59 0.668713082558007 1105 60-61 0.4575864777929368 1105 62-63 0.7415759143116958 1105 64-65 0.7462759230570484 1105 66-67 0.9586914797982544 1105 68-69 1.0230593121741478 1105 70-71 0.7523642173896974 1105 72-73 0.6622600540790557 1105 74-75 0.4857824113686853 1105 76-77 0.680410904374682 1105 78-79 0.5739822042618066 1105 80-81 0.5387591952115294 1105 82-83 0.526368102848668 1105 84-85 0.6874912074804449 1105 86-87 0.7427261308287463 1105 88-89 0.873843657607491 1105 90-91 1.0532694650809695 1105 92-93 0.8330792940078844 1105 94-95 0.6433786570670108 1105 96-97 0.6074291940795611 1105 98-99 0.6195427474076531 1105 100-101 0.2940492065690492 1106 1 -0.014438736925967532 1106 2 -0.07491907074003734 1106 3 -0.07589323261139924 1106 4 -0.16447829201575814 1106 5 -0.18528872035359 1106 6 -0.0857439955893824 1106 7 -0.10000674385200625 1106 8 -0.2213813205981907 1106 9 -0.20483923284302819 1106 10-11 -0.25910342956692745 1106 12-13 -0.4226736580495256 1106 14-15 -0.19352732542172646 1106 16-17 -0.08775662272658735 1106 18-19 -0.12831370997073321 1106 20-21 -0.16973649900837273 1106 22-23 -0.21122394308638803 1106 24-25 -0.15378919966951088 1106 26-27 -0.2510443090026584 1106 28-29 -0.21710441170325367 1106 30-31 -0.24197036291320018 1106 32-33 -0.13810674141023327 1106 34-35 -0.6896527897924543 1106 36-37 0.06413421487356885 1106 38-39 -0.05044535037520603 1106 40-41 -0.14587160319084802 1106 42-43 -0.23610150800087837 1106 44-45 -0.09618833457346909 1106 46-47 -0.36829925782123496 1106 48-49 -0.0833286845493646 1106 50-51 0.1451108746544989 1106 52-53 -0.07140345817643379 1106 54-55 -0.1661053065226028 1106 56-57 -0.054445337707349495 1106 58-59 0.04303777747093562 1106 60-61 0.025672313525831214 1106 62-63 -0.2130980881564284 1106 64-65 -0.2800052712388563 1106 66-67 -0.39917565306887326 1106 68-69 -0.06481004885697672 1106 70-71 -0.05015103696378986 1106 72-73 -0.132339219196691 1106 74-75 -0.32699096721351495 1106 76-77 -0.2723023137797149 1106 78-79 -0.23987526180154006 1106 80-81 -0.24898847378614164 1106 82-83 -0.41642892855130853 1106 84-85 -0.5211712085349127 1106 86-87 -0.2195896171487668 1106 88-89 -0.21981378788818873 1106 90-91 -0.34843165242897456 1106 92-93 -0.4148639392294662 1106 94-95 -0.36876055176337275 1106 96-97 -0.45023226211130307 1106 98-99 -0.7138831404299992 1106 100-101 -0.9637013761815361 1107 1 0.12198810739183585 1107 2 0.08830192634035683 1107 3 0.037194827105068384 1107 4 0.02095177289780281 1107 5 -0.03409529398081901 1107 6 0.03976292217004129 1107 7 0.08847335567543979 1107 8 0.026722942427163332 1107 9 -0.18283082518182425 1107 10-11 -0.0662258623708496 1107 12-13 0.003422197314613129 1107 14-15 0.15008315305244935 1107 16-17 -0.042666275484165794 1107 18-19 -0.21241038804159018 1107 20-21 -0.1429774617758781 1107 22-23 -0.04007887581506253 1107 24-25 -0.08071378154387787 1107 26-27 0.02164182838935602 1107 28-29 0.12334968226591059 1107 30-31 -0.01178806314696601 1107 32-33 -0.07581981743033595 1107 34-35 0.02811667173597243 1107 36-37 0.12244806744853776 1107 38-39 0.2180951478639983 1107 40-41 0.1293358292619189 1107 42-43 0.07824268439208737 1107 44-45 0.1911118831428169 1107 46-47 -0.18902946018702238 1107 48-49 0.0538737113330896 1107 50-51 0.13682360955231587 1107 52-53 0.21421522729878006 1107 54-55 0.07820748255345933 1107 56-57 0.09501137487704625 1107 58-59 0.23841657371055902 1107 60-61 0.37783994503349305 1107 62-63 0.2291117823174318 1107 64-65 0.2162514132531257 1107 66-67 -0.03216217873833216 1107 68-69 0.12410247334105406 1107 70-71 0.2827772972557909 1107 72-73 0.4378757881775712 1107 74-75 0.4623038126121095 1107 76-77 0.27722462986487884 1107 78-79 0.34326125734645885 1107 80-81 0.31379207421678856 1107 82-83 0.328122646129394 1107 84-85 0.4232037851944561 1107 86-87 0.48099430395165754 1107 88-89 0.4965570722416359 1107 90-91 0.5806038744544715 1107 92-93 0.2817880458012816 1107 94-95 0.319027270170789 1107 96-97 0.6101192945099783 1107 98-99 0.4585551816496434 1107 100-101 0.7401874179511587 1108 1 0.05172774318663187 1108 2 -0.027528426518969695 1108 3 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54-55 0.4784143843128774 2316 56-57 0.22755687061064123 2316 58-59 0.36771205056626144 2316 60-61 0.8273800794441257 2316 62-63 0.6615062548689679 2316 64-65 0.7643009488568353 2316 66-67 0.7936914797982482 2316 68-69 0.6358082801824025 2316 70-71 0.5130363020130204 2316 72-73 0.460358609290612 2316 74-75 0.4379483040415444 2316 76-77 0.8233546608246307 2316 78-79 0.7424729163361086 2316 80-81 0.34878112503608705 2316 82-83 0.44714854660511705 2316 84-85 0.5049163880789997 2316 86-87 0.42762422164402025 2316 88-89 0.26153328609046156 2316 90-91 0.30841781389417733 2316 92-93 0.25998331877568504 2316 94-95 0.18002855386783523 2316 96-97 0.18948801760897283 2316 98-99 0.39845399611766297 2316 100-101 0.6818562241129094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 4.0 5 15.0 6 35.0 7 64.0 8 154.0 9 338.0 10 618.0 11 1126.0 12 1919.0 13 2743.0 14 3359.0 15 5007.0 16 7470.0 17 11418.0 18 10090.0 19 4995.0 20 3673.0 21 3469.0 22 3511.0 23 3700.0 24 4232.0 25 4980.0 26 6047.0 27 7371.0 28 8999.0 29 11361.0 30 14373.0 31 17662.0 32 22822.0 33 29997.0 34 41968.0 35 61298.0 36 91737.0 37 142944.0 38 116595.0 39 10091.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46706783970149 21.141605545698976 15.745889195763905 19.645437418835623 2 15.167710571633217 22.37141681525168 41.33833188558192 21.122540727533185 3 19.248388424084485 28.154650329934928 31.17191667047654 21.425044575504046 4 11.998201740349595 18.288148249744737 38.934150169920294 30.77949983998537 5 11.448556809796496 40.50186916457796 34.385767516176045 13.663806509449497 6 28.29652996845426 38.769716088328074 18.19137749737119 14.742376445846478 7 26.338865267681616 35.85623066489889 21.250704826346027 16.55419924107347 8 26.20567213764306 35.61514195583596 20.01950654536034 18.159679361160638 9 27.600847315564092 16.85944619698563 21.717490361023483 33.822216126426795 10-11 24.085402093905728 28.6976333074262 27.104725765403316 20.112238833264755 12-13 25.62378078517435 26.737685930260913 28.351088149231895 19.287445135332845 14-15 22.915847544156417 27.121870190036425 28.309712126061047 21.65257013974611 16-17 19.555235526295736 30.646230512504 29.208232371721603 20.590301589478656 18-19 19.413127295447964 28.279004556607077 33.47711790792301 18.830750240021946 20-21 20.591444551120862 27.067846203081423 33.80385254270867 18.536856703089043 22-23 20.316755183370542 27.13076342799668 33.46761964994628 19.0848617386865 24-25 19.923528129852834 27.461752635292573 33.966738241573694 18.6479809932809 26-27 19.778902635932283 27.41070877493498 34.034638282243556 18.775750306889176 28-29 19.295273886592785 27.380963266400887 34.482325079550854 18.84143776745547 30-31 19.643929349731025 27.6720157271522 34.05400874746643 18.630046175650346 32-33 19.42242338347125 27.79301726634054 33.86122921714747 18.923330133040736 34-35 19.62571816089852 27.587817552842925 33.970191560371234 18.81627272588732 36-37 19.94917630564318 27.441747054968836 33.73116018226428 18.8779164571237 38-39 19.76096862189305 27.841783629741386 33.8431704232006 18.554077325164968 40-41 19.66674108656609 27.842008331472833 33.87055356975494 18.620697012206133 42-43 19.406680464913535 28.005767220120646 33.35477243561411 19.232779879351707 44-45 19.691019369389963 28.32388485042442 32.88491138237401 19.10018439781161 46-47 20.037031957207514 28.358097502247826 32.16964598668069 19.43522455386397 48-49 20.055090751154392 28.83463630960545 31.6310062634298 19.47926667581036 50-51 20.43234429052561 28.986574010576206 31.15553422027157 19.42554747862662 52-53 20.304637376369648 29.331900821408436 31.056553742056416 19.3069080601655 54-55 20.088480538015315 29.20650208514461 31.09780025434726 19.607217122492816 56-57 20.351651198585778 29.657797284323138 30.46282326765114 19.52772824943995 58-59 20.358204178667762 29.09782227708438 30.896767704475835 19.647205839772017 60-61 20.457946631311053 29.327100382511162 30.88152821591307 19.33342477026471 62-63 20.464956796049925 29.034349807220472 31.35273320227373 19.147960194455873 64-65 20.29526509090355 29.008137886892516 31.139456559837853 19.557140462366082 66-67 20.38144439872598 29.40428839208156 30.579633947484723 19.634633261707737 68-69 20.199027720629694 29.355217238909464 30.85333516207196 19.59241987838888 70-71 20.271415291302823 29.43453877687865 30.721208796232798 19.57283713558573 72-73 20.401560523628827 29.27414315975556 30.409713650009905 19.91458266660571 74-75 20.25480424876941 29.755482406010454 30.128087901370034 19.8616254438501 76-77 20.255261433426295 29.399030768527407 30.65674575961231 19.688962038433992 78-79 20.171063259117027 29.055227906551455 30.57521449580152 20.198494338529997 80-81 20.084502964080524 29.800591292156238 30.127935506484405 19.986970237278836 82-83 20.3885181857874 29.676440579683643 30.070841248396984 19.86419998613197 84-85 20.140736676877125 29.25036955759765 30.085264938508665 20.523628827016566 86-87 20.00099056675658 29.439644005547173 30.33161127112574 20.227754156570505 88-89 20.365061948521006 28.913272070589315 30.459775369938587 20.2618906109511 90-91 20.01162917413812 29.09793121107256 30.87522871598219 20.015210898807133 92-93 20.00003047937194 29.4717239056572 30.504517423913963 20.023728191056893 94-95 19.727975129154665 29.39727822734269 30.327268016885355 20.54747862661729 96-97 20.219372437860986 29.10094637223975 29.936679925021714 20.74300126487755 98-99 19.926819251017434 29.175680278458692 29.99816362343765 20.899336847086218 100-101 19.961437335670464 29.15230728194185 30.28358038334032 20.602674999047366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 793.0 1 549.0 2 463.5 3 596.5 4 447.5 5 388.5 6 702.5 7 1416.5 8 2126.0 9 2441.0 10 2139.5 11 1718.0 12 1691.5 13 1794.0 14 1935.0 15 2012.0 16 2058.0 17 2152.0 18 2256.5 19 2421.0 20 2637.0 21 2901.0 22 3239.5 23 3722.5 24 4287.5 25 5034.5 26 6289.5 27 7618.5 28 8217.0 29 9044.5 30 10641.5 31 12223.0 32 14518.0 33 17248.5 34 20173.0 35 22432.5 36 25204.0 37 27731.5 38 27496.5 39 26591.0 40 25251.5 41 23827.0 42 22386.5 43 22092.5 44 23007.5 45 23313.0 46 22312.5 47 21423.0 48 24041.5 49 23224.5 50 19500.0 51 17187.0 52 14498.0 53 13215.0 54 12405.5 55 11214.5 56 9529.0 57 8056.0 58 7088.5 59 6134.5 60 5049.5 61 4062.0 62 3104.5 63 2170.5 64 1496.0 65 1044.0 66 712.0 67 505.5 68 360.0 69 287.0 70 213.0 71 140.5 72 102.0 73 79.0 74 57.0 75 38.5 76 28.5 77 21.5 78 14.0 79 11.5 80 10.5 81 9.5 82 6.5 83 1.5 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01737301696155077 2 0.0 3 0.0 4 0.0 5 0.04297535774699401 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0045718465688291505 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.619744281381917E-4 24-25 1.5239488562763834E-4 26-27 0.0012953565278349258 28-29 9.1436931376583E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.333820996967343E-4 42-43 0.010362852222679407 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 7.619744281381917E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.857769853243725E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012420183178652523 92-93 0.0012953565278349258 94-95 0.0 96-97 0.0 98-99 9.905667565796492E-4 100-101 0.01752541184717841 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 656190.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.72555680980054 #Duplication Level Percentage of deduplicated Percentage of total 1 83.81102291355138 47.54226941570153 2 9.69495928093874 10.99903926919187 3 2.5281983146517772 4.30240371372664 4 1.0534092850228838 2.390209129661478 5 0.5590126144664275 1.5855150909655225 6 0.386368486361885 1.315018051358265 7 0.27770482749377584 1.1027072677847827 8 0.20225267726199841 0.9178316587167796 9 0.15102348732519943 0.7710202268891813 >10 1.1347397403722308 12.87300579176649 >50 0.12991102485557132 5.124081865764859 >100 0.067371181474515 6.873825246595302 >500 0.0021472886525724225 0.8244753402360349 >1k 0.001342055407857764 1.4481227998125443 >5k 5.368221631431056E-4 1.9304751318287505 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6682 1.0183026257638794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5997 0.913912129108947 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4196 0.6394489400935705 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1424 0.217010317133757 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1302 0.1984181410871851 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1295 0.19735137688779164 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1294 0.19719898200216399 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 865 0.13182157606790715 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.11764885170453679 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 752 0.11460095399198403 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 658 0.10027583474298604 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 1.5239488562763834E-4 0.0 0.0 0.0 0.0 20-21 1.5239488562763834E-4 0.0 0.0 0.0 0.0 22-23 1.5239488562763834E-4 0.0 0.0 1.5239488562763834E-4 0.0 24-25 1.5239488562763834E-4 0.0 0.0 5.333820996967342E-4 0.0 26-27 1.5239488562763834E-4 0.0 0.0 9.905667565796492E-4 0.0 28-29 1.5239488562763834E-4 0.0 0.0 0.0025907130556698517 0.0 30-31 1.5239488562763834E-4 0.0 0.0 0.004495649126015331 0.0 32-33 1.5239488562763834E-4 0.0 0.0 0.007162559624499002 0.0 34-35 1.5239488562763834E-4 0.0 0.0 0.010286654779865588 0.0 36-37 1.5239488562763834E-4 0.0 0.0 0.014248921806184185 0.0 38-39 1.5239488562763834E-4 0.0 0.0 0.021487678873497007 0.0 40-41 1.5239488562763834E-4 0.0 0.0 0.027812066627043996 0.0 42-43 1.5239488562763834E-4 0.0 0.0 0.039089288163489236 0.0 44-45 1.5239488562763834E-4 0.0 0.0 0.04792819152989226 0.0 46-47 1.5239488562763834E-4 0.0 0.0 0.06781572410429906 0.0 48-49 1.5239488562763834E-4 0.0 0.0 0.0918179185906521 0.0 50-51 1.5239488562763834E-4 0.0 0.0 0.10393331199804934 0.0 52-53 1.5239488562763834E-4 0.0 0.0 0.12275408037306268 0.0 54-55 1.5239488562763834E-4 0.0 0.0 0.155899967997074 0.0 56-57 1.5239488562763834E-4 0.0 0.0 0.20611408281138086 0.0 58-59 1.5239488562763834E-4 0.0 0.0 0.26059525442326154 0.0 60-61 1.5239488562763834E-4 1.5239488562763834E-4 0.0 0.33115408646885813 0.0 62-63 1.5239488562763834E-4 1.5239488562763834E-4 0.0 0.4013319313003856 0.0 64-65 1.5239488562763834E-4 1.5239488562763834E-4 0.0 0.47821515109952906 0.0 66-67 1.5239488562763834E-4 1.5239488562763834E-4 0.0 0.5710998338895747 0.0 68-69 1.5239488562763834E-4 1.5239488562763834E-4 0.0 0.6815861259696124 0.0 70-71 1.5239488562763834E-4 1.5239488562763834E-4 0.0 0.7637269693229095 0.0 72-73 1.5239488562763834E-4 1.5239488562763834E-4 0.0 0.8723845227754157 0.0 74-75 1.5239488562763834E-4 1.5239488562763834E-4 0.0 0.9953671954769198 0.0 76-77 1.5239488562763834E-4 1.5239488562763834E-4 0.0 1.1213215684481628 0.0 78-79 1.5239488562763834E-4 1.5239488562763834E-4 0.0 1.2687636202929031 0.0 80-81 1.5239488562763834E-4 1.5239488562763834E-4 0.0 1.4812020908578307 0.0 82-83 1.5239488562763834E-4 1.5239488562763834E-4 0.0 1.7181761380088085 0.0 84-85 1.5239488562763834E-4 1.5239488562763834E-4 0.0 1.9951538426370412 0.0 86-87 1.5239488562763834E-4 1.5239488562763834E-4 0.0 2.356710708788613 0.0 88-89 1.5239488562763834E-4 1.5239488562763834E-4 0.0 2.759566588945275 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 60 1.4886791E-6 47.51212 2 TATCACG 55 4.9535083E-5 43.192837 2 TACCTGG 490 0.0 41.69431 2 GGACCGA 70 4.308835E-6 40.71226 6 ATCACGC 60 8.274411E-5 39.593433 3 CGTGCAA 110 6.998562E-8 34.549004 9 GTACCTG 630 0.0 33.18813 1 AGGACCG 125 2.1467167E-7 30.407757 5 GACCGAG 95 3.48614E-5 30.00308 7 ACCGTGC 115 3.8581584E-6 28.916012 7 ACCTGGG 765 0.0 27.948307 3 TAACGCA 105 6.8894165E-5 27.149782 4 GGTATCA 5720 0.0 26.750412 1 CCGTGCA 125 7.3786887E-6 26.602732 8 TCACGCA 90 8.930126E-4 26.395622 4 GTATAGG 325 0.0 26.318417 1 TGACCGT 130 9.99272E-6 25.583448 5 GTATCAA 7780 0.0 25.408812 1 CTGACCG 160 1.853412E-6 23.756062 4 GTACATG 5420 0.0 23.233639 1 >>END_MODULE