FastQCFastQC Report
Thu 26 May 2016
SRR937722_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937722_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563469
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52740.9359876053518472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40460.7180519247731464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.4720756598854596No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10030.17800446874628417No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.17250283511604012No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8850.15706276654083898No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8550.15173860496318342No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.14694685954329342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6920.12281066039125489No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6650.1180189149713649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG3700.039.8259052
GAACCGT500.00161505638.0002866
GTACCTG4450.033.12841
GTACACG753.0555448E-431.7035241
TATAACG650.005821093529.251782
GGTATCA40200.028.7461031
TAACGCA700.00835864327.1623674
ACCTGGG5300.026.9037273
GGATAGG1257.3157207E-626.6309571
GTATCAA56450.026.5366081
GTACATG45550.025.9961931
TACATGG46150.025.5438082
CATGGGG13750.024.8906044
ACATGGG45700.024.8570023
GGTCTAT1151.2705417E-424.8114531
GTATAGG2551.8189894E-1124.243871
ATGGGAT11100.023.1247185
GAGTACT35800.022.81937212-13
TAGGACA3150.022.6353054
TATCAAC67350.022.0202712
Produced by FastQC (version 0.11.5)