##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937722_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 563469 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.755959955206055 33.0 31.0 34.0 30.0 34.0 2 31.881640338687664 33.0 31.0 34.0 30.0 34.0 3 31.899948355632695 33.0 31.0 34.0 30.0 34.0 4 35.48371960125579 37.0 35.0 37.0 33.0 37.0 5 35.485563535882186 37.0 35.0 37.0 33.0 37.0 6 35.58249344684446 37.0 35.0 37.0 33.0 37.0 7 35.54037577932415 37.0 35.0 37.0 33.0 37.0 8 35.53463633314344 37.0 35.0 37.0 33.0 37.0 9 37.141853411634 39.0 37.0 39.0 34.0 39.0 10-11 37.03948309489963 39.0 37.0 39.0 33.0 39.0 12-13 36.92307828824656 39.0 37.0 39.0 33.0 39.0 14-15 38.03057665994048 40.0 38.0 41.0 33.0 41.0 16-17 37.99245300096368 40.0 38.0 41.0 33.0 41.0 18-19 38.02011823188143 40.0 38.0 41.0 33.0 41.0 20-21 37.861616166994104 40.0 38.0 41.0 33.0 41.0 22-23 37.75165803265131 40.0 37.5 41.0 33.0 41.0 24-25 37.6255561530448 40.0 37.0 41.0 32.0 41.0 26-27 37.69436206783337 40.0 37.0 41.0 33.0 41.0 28-29 37.631547609540185 40.0 37.0 41.0 33.0 41.0 30-31 37.38082396724576 39.5 37.0 41.0 32.0 41.0 32-33 37.096351352070826 39.0 36.0 41.0 32.0 41.0 34-35 36.83150448383141 39.0 35.0 41.0 31.0 41.0 36-37 36.80319414200249 39.0 35.0 41.0 31.0 41.0 38-39 36.72995320061973 39.0 35.0 41.0 31.0 41.0 40-41 36.527870211138506 39.0 35.0 41.0 30.5 41.0 42-43 36.243298211614125 39.0 35.0 41.0 30.0 41.0 44-45 35.903062990155625 39.0 35.0 41.0 29.5 41.0 46-47 35.37817785894166 39.0 35.0 41.0 26.0 41.0 48-49 34.92525054617025 39.0 35.0 40.5 24.0 41.0 50-51 34.00623636792796 38.0 34.0 39.5 22.5 40.5 52-53 34.09229079150761 38.0 34.0 39.5 22.5 40.5 54-55 34.418325586678236 39.0 35.0 40.0 22.0 41.0 56-57 34.207604145037266 38.0 34.0 40.0 19.5 41.0 58-59 34.002882146134034 38.0 34.0 40.0 18.5 41.0 60-61 33.70590662485425 38.0 34.0 40.0 16.5 41.0 62-63 33.36272093052146 38.0 33.0 40.0 13.0 41.0 64-65 33.01576395507118 37.0 33.0 40.0 8.5 41.0 66-67 32.61394681872472 37.0 32.5 39.5 7.5 41.0 68-69 32.15571042950012 36.0 32.0 39.0 4.5 40.5 70-71 31.725792368346795 36.0 31.0 39.0 2.0 40.0 72-73 31.25085319689282 35.0 31.0 38.0 2.0 40.0 74-75 30.76159203079495 35.0 30.5 37.5 2.0 39.5 76-77 30.291829719114983 35.0 30.0 37.0 2.0 39.0 78-79 29.77065109171933 34.5 29.5 36.5 2.0 39.0 80-81 29.23683379209859 34.0 29.0 36.0 2.0 38.5 82-83 28.719192182711026 34.0 29.0 35.5 2.0 37.0 84-85 28.24562132078251 34.0 27.5 35.0 2.0 37.0 86-87 27.737417675151605 34.0 26.5 35.0 2.0 36.0 88-89 27.24902346003063 33.5 26.0 35.0 2.0 36.0 90-91 26.74056159256321 33.0 25.0 35.0 2.0 35.5 92-93 26.20856249412124 33.0 24.5 35.0 2.0 35.0 94-95 25.594976831023533 32.0 21.5 35.0 2.0 35.0 96-97 24.850841838681454 32.0 17.5 34.0 2.0 35.0 98-99 23.973919594511855 31.5 2.0 34.0 2.0 35.0 100-101 21.491488440357855 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15538579756753634 1101 2 0.146603780156493 1101 3 0.06547048722583426 1101 4 0.051055111361272054 1101 5 0.10847696826299824 1101 6 0.058259797131029245 1101 7 -0.08810722716078345 1101 8 -0.06728822626534736 1101 9 0.22107762188999658 1101 10-11 -0.018970102823303137 1101 12-13 0.007588602812489853 1101 14-15 0.17673779155534675 1101 16-17 0.22781971836504766 1101 18-19 0.10380541610038563 1101 20-21 0.16480229727501694 1101 22-23 0.28357245422584043 1101 24-25 0.040033980889568 1101 26-27 0.33226386441015165 1101 28-29 0.03593210796183399 1101 30-31 0.059722785122325206 1101 32-33 0.007047608533078176 1101 34-35 -0.05347144036063867 1101 36-37 0.10755403177175538 1101 38-39 0.25696388480975685 1101 40-41 0.42345687476478133 1101 42-43 0.4778486707698377 1101 44-45 0.5510758767447044 1101 46-47 0.6150670088544103 1101 48-49 0.8682972239829994 1101 50-51 0.6809389709606108 1101 52-53 0.740214316783856 1101 54-55 1.0629303468351878 1101 56-57 1.063685518758973 1101 58-59 0.8722291135890927 1101 60-61 0.8947845376638668 1101 62-63 1.2288161715893935 1101 64-65 0.8941876961125743 1101 66-67 0.9356455913112995 1101 68-69 0.8546882002814442 1101 70-71 0.8847883876725895 1101 72-73 0.7487616204869312 1101 74-75 0.9947755895128552 1101 76-77 0.9296117206571743 1101 78-79 0.7181113853164511 1101 80-81 0.8079241917956317 1101 82-83 0.951073817324616 1101 84-85 0.9620650464926292 1101 86-87 0.7264090285063887 1101 88-89 0.7880813198563921 1101 90-91 1.0298875575930673 1101 92-93 1.327706078660455 1101 94-95 1.2936970812722066 1101 96-97 1.553848238550426 1101 98-99 1.6113738025033193 1101 100-101 1.736851668851152 1102 1 -0.0024644916017564356 1102 2 -0.016333259801449174 1102 3 0.03438478796190125 1102 4 -0.056302275599826146 1102 5 0.06331726794753934 1102 6 -0.14713255302670092 1102 7 -7.386677496370453E-4 1102 8 -0.03225930933579946 1102 9 -0.12871535918256427 1102 10-11 0.01131380884862665 1102 12-13 -0.09830815060496434 1102 14-15 0.04748404549855678 1102 16-17 0.04849696599912079 1102 18-19 -0.0747730539311533 1102 20-21 -0.1097557337449615 1102 22-23 0.0060060767284682015 1102 24-25 0.16878660970134973 1102 26-27 0.3162708307613613 1102 28-29 0.12982669260957636 1102 30-31 -8.74612354010651E-4 1102 32-33 -0.07798623741854982 1102 34-35 -0.10329399556568575 1102 36-37 0.16619789349626046 1102 38-39 0.18497058827978918 1102 40-41 0.2964491197700312 1102 42-43 0.5128808737141028 1102 44-45 0.13574333205490774 1102 46-47 0.2598685335652391 1102 48-49 0.4235072003236908 1102 50-51 0.24564881586071863 1102 52-53 0.20854206389216046 1102 54-55 0.5596771922610557 1102 56-57 0.6857248194950429 1102 58-59 0.791907412747868 1102 60-61 0.6043714856133988 1102 62-63 0.5042072073412314 1102 64-65 0.5728138133575769 1102 66-67 0.801024140207204 1102 68-69 0.7160364521216138 1102 70-71 0.7089767420364232 1102 72-73 0.5303944412019703 1102 74-75 0.7295449112794188 1102 76-77 0.6130764945793601 1102 78-79 0.7350245030872173 1102 80-81 0.7843174620374853 1102 82-83 0.5213567431921255 1102 84-85 0.4923571732013521 1102 86-87 0.7756203849732657 1102 88-89 0.6496020874694324 1102 90-91 0.9527237168990617 1102 92-93 1.0144240071567765 1102 94-95 0.9228719629756732 1102 96-97 0.867797371042542 1102 98-99 1.0102910606526372 1102 100-101 1.478294886516764 1103 1 0.2518716034283415 1103 2 0.3512855567132682 1103 3 0.07961883887418608 1103 4 0.07659311502427357 1103 5 0.2843812722923005 1103 6 0.23794157368780589 1103 7 0.3742096226560676 1103 8 0.1876110411339198 1103 9 0.17777405046142292 1103 10-11 0.3185756847224894 1103 12-13 0.3032674031787863 1103 14-15 0.50066750767256 1103 16-17 0.31948066158848576 1103 18-19 0.2900805992505724 1103 20-21 0.3219165372017585 1103 22-23 0.5093622893906726 1103 24-25 0.2054414900470789 1103 26-27 0.15546670323799106 1103 28-29 0.15756672334645572 1103 30-31 0.25314082541536465 1103 32-33 0.3087131305111015 1103 34-35 0.435287717148519 1103 36-37 0.4316096636398896 1103 38-39 0.45161818883906335 1103 40-41 0.5266047685376734 1103 42-43 0.6289819125280829 1103 44-45 0.4568680012868285 1103 46-47 0.7203554703928745 1103 48-49 0.676384449257732 1103 50-51 0.32708709367123845 1103 52-53 0.2813601501171874 1103 54-55 0.3273763175311615 1103 56-57 0.6437793832278444 1103 58-59 0.7219486648711424 1103 60-61 0.37498714755398055 1103 62-63 0.5941206587688797 1103 64-65 0.9551366429990154 1103 66-67 1.1516712323369447 1103 68-69 1.0241765244572747 1103 70-71 1.1835864645956669 1103 72-73 1.0443831864210011 1103 74-75 0.8915132268754924 1103 76-77 1.0372124532579043 1103 78-79 1.0056228322028993 1103 80-81 1.0831989170703586 1103 82-83 0.7633391561524547 1103 84-85 1.0194997992398775 1103 86-87 0.8998522519496106 1103 88-89 1.1607690487941191 1103 90-91 1.2051451125381227 1103 92-93 1.0937546134589908 1103 94-95 1.1033754237630475 1103 96-97 1.3341023594295471 1103 98-99 1.3977004966425177 1103 100-101 1.4175751120745943 1104 1 0.11718196873870923 1104 2 0.14016694559465748 1104 3 0.1500835093388595 1104 4 0.2200226584538143 1104 5 0.1551267430377692 1104 6 0.286773822520054 1104 7 0.23905288749933362 1104 8 0.3798350333579137 1104 9 0.4020281747155465 1104 10-11 0.17926904541585031 1104 12-13 0.3294776793890648 1104 14-15 0.42197862898368044 1104 16-17 0.5581163502241822 1104 18-19 0.548535657705628 1104 20-21 0.7385418038270188 1104 22-23 0.7297257097540921 1104 24-25 0.5694207540263463 1104 26-27 0.5763849741562623 1104 28-29 0.38419684997658266 1104 30-31 0.4550672773418185 1104 32-33 0.2401749119728791 1104 34-35 0.32106275292355235 1104 36-37 0.36485209688232345 1104 38-39 0.5774005146213881 1104 40-41 0.6767303186632248 1104 42-43 0.6667077002538591 1104 44-45 0.33850933292816165 1104 46-47 0.3595541095915067 1104 48-49 0.232436505309785 1104 50-51 0.24020775679190365 1104 52-53 0.3704264891835294 1104 54-55 0.6110210511758893 1104 56-57 0.5067879962364472 1104 58-59 0.5230826660051449 1104 60-61 0.5754164479832866 1104 62-63 0.6751639398121654 1104 64-65 0.7008382387006193 1104 66-67 0.4896791703448855 1104 68-69 0.6305903808711264 1104 70-71 0.7899557709649763 1104 72-73 0.8311518231896358 1104 74-75 0.8093102946725637 1104 76-77 0.7684528344982873 1104 78-79 0.62786005444012 1104 80-81 1.131265715137154 1104 82-83 1.1354859282992287 1104 84-85 0.9899712197112969 1104 86-87 1.0409462930436533 1104 88-89 0.849808387833459 1104 90-91 1.055974013367024 1104 92-93 1.3768512465556242 1104 94-95 1.1986814690690935 1104 96-97 1.2556065309337185 1104 98-99 1.5124884114304287 1104 100-101 0.9360633805628638 1105 1 0.10645339784416308 1105 2 -0.006599020673384359 1105 3 -0.03662149064415132 1105 4 0.033234399051451646 1105 5 0.18878903603616237 1105 6 0.13206252880461022 1105 7 0.18248405914626886 1105 8 0.07871799780104283 1105 9 0.084334018847116 1105 10-11 0.2443770617686738 1105 12-13 0.20587482342106256 1105 14-15 0.2591699319426155 1105 16-17 0.22397506068870143 1105 18-19 0.1962803469440999 1105 20-21 0.3440229922957627 1105 22-23 0.5333260849312325 1105 24-25 0.5151636489393638 1105 26-27 0.3808850608140233 1105 28-29 0.3645195906727636 1105 30-31 0.31001237710019325 1105 32-33 0.4408844861264427 1105 34-35 0.37086293031709516 1105 36-37 0.5687595124079934 1105 38-39 0.6823745003007673 1105 40-41 0.8255013076831759 1105 42-43 0.7122582489164486 1105 44-45 0.550375669275823 1105 46-47 0.9662858885224566 1105 48-49 0.9590626977036081 1105 50-51 1.0553446590657387 1105 52-53 0.734679652191879 1105 54-55 0.8935406511228834 1105 56-57 1.0497116252596683 1105 58-59 1.078550431016474 1105 60-61 0.8155119754232061 1105 62-63 0.8965677276059978 1105 64-65 0.9352211504694168 1105 66-67 0.9891379841190542 1105 68-69 0.8697794001431305 1105 70-71 0.8603286712133915 1105 72-73 0.913027267098272 1105 74-75 0.6750357900661044 1105 76-77 0.8060640028148498 1105 78-79 0.7571457905723271 1105 80-81 0.949902061781799 1105 82-83 1.0770701001738558 1105 84-85 0.7711093929656556 1105 86-87 0.8846386273998874 1105 88-89 0.6763679726918035 1105 90-91 0.649646807247283 1105 92-93 1.2075729527959993 1105 94-95 1.1774129353524252 1105 96-97 1.0596962675960704 1105 98-99 1.2154561849376222 1105 100-101 1.3419173673297138 1106 1 -0.14367297640861665 1106 2 0.06674536906265871 1106 3 0.09531107820561502 1106 4 0.08169112743177465 1106 5 0.14732967692033583 1106 6 0.20565644046954645 1106 7 -0.05549317843087209 1106 8 0.0476353092452797 1106 9 0.049824705871330366 1106 10-11 -0.17594262744745492 1106 12-13 -0.30878889200347714 1106 14-15 -0.1877410511092492 1106 16-17 -0.15759451828606785 1106 18-19 -0.17025983791723576 1106 20-21 0.03266372158813624 1106 22-23 -0.3768911927415033 1106 24-25 -0.24324427209124622 1106 26-27 -0.3423295129874617 1106 28-29 0.15112456519356243 1106 30-31 -0.03144977838208263 1106 32-33 0.21278487525158596 1106 34-35 -0.28655529209729025 1106 36-37 0.004251640636454113 1106 38-39 0.07109847527113544 1106 40-41 -0.10801830667638512 1106 42-43 0.09795461944790418 1106 44-45 0.0990184635772593 1106 46-47 -0.11521098492475801 1106 48-49 0.183224809519551 1106 50-51 -0.11612638025763289 1106 52-53 -0.38077048751889464 1106 54-55 -0.42639378483925583 1106 56-57 -0.40394505407669357 1106 58-59 -0.2390275284534269 1106 60-61 -0.4705950307653666 1106 62-63 -0.20918117159359895 1106 64-65 -0.1684734949190485 1106 66-67 0.21093071313607936 1106 68-69 0.061975816922199556 1106 70-71 -0.15710022041450245 1106 72-73 0.039413350658008994 1106 74-75 0.02444607940399024 1106 76-77 0.15176050240937755 1106 78-79 0.1615616833983573 1106 80-81 -0.05498405081711866 1106 82-83 0.01725367087567875 1106 84-85 0.2965348170991611 1106 86-87 0.08835822498124912 1106 88-89 0.18709311353187275 1106 90-91 0.6122529347314831 1106 92-93 0.19521839073924951 1106 94-95 0.049090905792684936 1106 96-97 0.1409059757199458 1106 98-99 0.4714721371326611 1106 100-101 0.6300022650149657 1107 1 0.02640295443028151 1107 2 0.14531701545061182 1107 3 0.2613038205591671 1107 4 0.10038109175342669 1107 5 0.14113628198848716 1107 6 0.21628675791534135 1107 7 0.1165496355843132 1107 8 0.09080000902877572 1107 9 0.11783007287038316 1107 10-11 0.193774995215918 1107 12-13 0.16172463222425648 1107 14-15 0.28945225233965033 1107 16-17 0.22233564973760167 1107 18-19 0.22457747492391888 1107 20-21 0.34963685609854167 1107 22-23 0.18890333657878244 1107 24-25 0.26393103971309984 1107 26-27 0.3176192565670135 1107 28-29 0.2843389707069335 1107 30-31 0.1412791576713488 1107 32-33 0.38183314774876465 1107 34-35 0.43660208905112086 1107 36-37 0.3536631003992099 1107 38-39 0.1936673161823066 1107 40-41 0.322047561039291 1107 42-43 0.47938053351493437 1107 44-45 0.48918862184274303 1107 46-47 0.43332681277597374 1107 48-49 0.42463913574771084 1107 50-51 0.40927843174493006 1107 52-53 0.2754777971760092 1107 54-55 0.3606019154626452 1107 56-57 0.4979379697393682 1107 58-59 0.5785097508439847 1107 60-61 0.5141166455070092 1107 62-63 0.5432156813933204 1107 64-65 0.3062894117988506 1107 66-67 0.6116259834681657 1107 68-69 0.8176477591049789 1107 70-71 0.3566633876725902 1107 72-73 0.5225667675457579 1107 74-75 0.7639545110814794 1107 76-77 0.5369646618336432 1107 78-79 0.4567756010027253 1107 80-81 0.8647869368936725 1107 82-83 0.8725505330108909 1107 84-85 0.6128309778651762 1107 86-87 0.7891541265456041 1107 88-89 0.7043803394642367 1107 90-91 1.095298096808751 1107 92-93 1.0030737257192754 1107 94-95 0.8510806597035803 1107 96-97 0.8298899052170938 1107 98-99 0.9506201260327352 1107 100-101 1.0400379433609572 1108 1 -0.1600231801711871 1108 2 -0.16440420168299497 1108 3 -0.13564989941739825 1108 4 -0.07018646743193102 1108 5 -0.19814832464854248 1108 6 -0.27845117240617157 1108 7 -0.09060429804537762 1108 8 -0.05612097377559877 1108 9 -0.3546638104474411 1108 10-11 -0.1226022059281604 1108 12-13 -0.31974095928475776 1108 14-15 -0.2799781208641079 1108 16-17 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-0.8925299004920859 2316 74-75 -0.7052899699277049 2316 76-77 -0.7486516826062299 2316 78-79 -0.4480235214434565 2316 80-81 -0.4882296543582143 2316 82-83 -0.2575399647266643 2316 84-85 -0.22870199896192034 2316 86-87 -0.11403386193650178 2316 88-89 -0.267702479677407 2316 90-91 -0.273925816532806 2316 92-93 -0.0019996322952557932 2316 94-95 -0.1955256751380503 2316 96-97 -0.2623012369740465 2316 98-99 0.2258223747643946 2316 100-101 0.1538388483383315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2464.0 3 393.0 4 375.0 5 384.0 6 478.0 7 549.0 8 641.0 9 816.0 10 1117.0 11 1483.0 12 2249.0 13 3168.0 14 3770.0 15 4655.0 16 6602.0 17 9440.0 18 7703.0 19 4478.0 20 4013.0 21 3924.0 22 4296.0 23 4656.0 24 5221.0 25 5790.0 26 6454.0 27 7568.0 28 9110.0 29 10869.0 30 13338.0 31 16350.0 32 20807.0 33 27003.0 34 37244.0 35 52540.0 36 75025.0 37 106916.0 38 89559.0 39 12017.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65464058015936 20.4607357449636 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28.334274571884688 33.31335239526683 19.289046206660412 20-21 20.42014804064893 27.16254237227848 33.124806590334316 19.292502996738268 22-23 19.797785404229092 27.26997845166833 32.94040014264764 19.991836001454935 24-25 19.466430931993866 27.680829719644123 33.28892556926722 19.563813779094794 26-27 19.280496459577286 27.571542151573848 33.45045809388123 19.697503294967635 28-29 18.83501847023903 27.492503831473858 33.884371767263424 19.788105931023686 30-31 19.144502130695084 27.735237009253026 33.65413095124795 19.46612990880394 32-33 18.899274675187204 27.85037351470308 33.39091573814298 19.859436071966737 34-35 19.290821018851076 27.790526609777228 33.27898773087987 19.639664640491834 36-37 19.464833571955786 27.59219571191008 33.14136824038915 19.801602475744993 38-39 19.29379985364557 28.054526362025385 33.19747089582961 19.454202888499438 40-41 19.18442178751826 27.80877372523671 33.526570220121116 19.480234267123915 42-43 18.877266995462897 28.055608418274335 32.90724993908436 20.159874647178405 44-45 19.10896451385412 28.345251572522805 32.503132595265136 20.042651318357944 46-47 19.407918724665933 28.327733678146405 31.821011424008027 20.443336173179638 48-49 19.51041617560335 28.764050340623754 31.387729361776312 20.337804121996584 50-51 19.716835380101095 28.823536213424795 31.18466449582093 20.274963910653184 52-53 19.45307710854736 29.294877669242975 31.073676043919672 20.178369178289994 54-55 19.44529920154817 29.076524377771413 31.110806758292675 20.367369662387745 56-57 19.696936018344235 29.56460892689957 30.365880006721714 20.372575048034477 58-59 19.67549372112625 29.071132117746217 30.838410134397414 20.414964026730114 60-61 19.81658422795046 29.123210024927193 31.02795330329743 20.03225244382492 62-63 19.82186627879581 29.01189840083288 31.21429835175631 19.951936968615 64-65 19.614885727265698 28.805334950200432 31.322829031417776 20.25695029111609 66-67 19.589728726279553 29.222559674528437 30.71975731884264 20.46795428034937 68-69 19.444417278275907 29.185652113997147 30.9713584615453 20.398572146181646 70-71 19.54596697618345 29.265250310287318 30.841047544195938 20.3477351693333 72-73 19.592890927842387 29.137327074220277 30.518777339165688 20.75100465877165 74-75 19.447023212469144 29.38977456232082 30.651325664610766 20.51187656059927 76-77 19.44355527102006 29.09615521411296 30.879125764689146 20.581163750177836 78-79 19.307242361134577 28.894572008344465 30.82178381579579 20.97640181472517 80-81 19.307795173095663 29.535981278496585 30.29218562171467 20.86403792669308 82-83 19.65342120772771 29.341057604012306 30.264929920644583 20.7405912676154 84-85 19.427594103131202 29.070364891869403 30.230253098842958 21.27178790615644 86-87 19.317684793701705 29.18330553767704 30.51634135673621 20.982668311885043 88-89 19.695359987482252 28.627057360266644 30.56037368321386 21.117208969037247 90-91 19.357847268899345 28.693983007749292 30.99298687350411 20.955182849847255 92-93 19.373075757050408 29.057346551765523 30.651506543870738 20.918071147313327 94-95 19.100556195678458 28.918174511728473 30.56249022034453 21.41877907224854 96-97 19.234920691372075 28.876253645351728 30.203961874955542 21.684863788320648 98-99 19.096966264059606 28.925107617285278 30.1804128982462 21.797513220408916 100-101 19.355420845967913 28.54939465138338 30.775480958565687 21.319703544083016 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1120.0 1 693.5 2 373.0 3 455.5 4 368.5 5 350.5 6 578.5 7 1124.5 8 1747.0 9 1895.0 10 1608.5 11 1410.5 12 1452.0 13 1506.5 14 1594.0 15 1673.0 16 1688.5 17 1740.5 18 1825.5 19 1952.5 20 2120.0 21 2434.5 22 2714.0 23 3056.0 24 3513.5 25 4189.0 26 5342.0 27 6528.5 28 7058.0 29 7746.5 30 9026.0 31 10491.5 32 12522.5 33 15072.0 34 17640.5 35 19369.0 36 21839.5 37 24106.5 38 23641.0 39 22541.5 40 21512.0 41 20472.0 42 19401.5 43 19269.0 44 19739.5 45 19677.5 46 18834.5 47 18228.5 48 20341.5 49 19847.0 50 16842.0 51 14716.5 52 12572.0 53 11533.0 54 10849.5 55 9675.5 56 8310.5 57 7094.5 58 6110.0 59 5286.5 60 4431.5 61 3597.5 62 2729.0 63 1929.0 64 1360.5 65 991.0 66 682.0 67 502.0 68 388.0 69 269.0 70 195.5 71 140.0 72 96.5 73 82.5 74 62.5 75 40.5 76 34.0 77 22.5 78 14.0 79 11.0 80 5.5 81 3.5 82 1.5 83 2.0 84 4.0 85 3.0 86 0.5 87 0.0 88 1.0 89 2.0 90 1.5 91 1.0 92 0.5 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3737561427514202 2 0.32069199902745316 3 0.31288322871355834 4 0.3212244151852187 5 0.3242414400792235 6 0.23674771815308382 7 0.22698675526071532 8 0.22645433910294976 9 0.24171693562556237 10-11 0.23763507841602644 12-13 0.23257712491725366 14-15 0.2360378299427298 16-17 0.21837936071017217 18-19 0.22476835460335884 20-21 0.21136921463292568 22-23 0.22086396944641143 24-25 0.22272742599859086 26-27 0.22148512163047124 28-29 0.23932106291561736 30-31 0.23577162186384698 32-33 0.2442015443618016 34-35 0.2353279417323757 36-37 0.2154510718424616 38-39 0.20151951571426288 40-41 0.19867996287284662 42-43 0.21580601594763862 44-45 0.21926672097311475 46-47 0.21864556878905494 48-49 0.23754634238973218 50-51 0.2347067895483159 52-53 0.26416715027800997 54-55 0.2229048980511794 56-57 0.19743765850472697 58-59 0.19646156221549013 60-61 0.1829736862187627 62-63 0.19211349692707141 64-65 0.18891899998047806 66-67 0.19255717705854272 68-69 0.19282338513742547 70-71 0.19291212116371972 72-73 0.19326706526889678 74-75 0.2103043823173946 76-77 0.2039153884242079 78-79 0.209417022054452 80-81 0.1989461709517294 82-83 0.17738331656222436 84-85 0.17764952464110714 86-87 0.1829736862187627 88-89 0.18945141613824362 90-91 0.1943318975844279 92-93 0.19033877640118624 94-95 0.19113740063783455 96-97 0.19681650632066716 98-99 0.20861839781780364 100-101 0.18182011787693733 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 563469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.10873779549147 #Duplication Level Percentage of deduplicated Percentage of total 1 85.66039718419158 51.48938353802228 2 8.539790038701248 10.266320005296866 3 2.2138942004841224 3.99223158011578 4 0.936875353438712 2.252575798676238 5 0.5046548758216451 1.516708380398979 6 0.33411911361731556 1.2050086915731146 7 0.22917068380048267 0.9642612380083688 8 0.17414073248622916 0.8373903702823657 9 0.14200834958619662 0.7682348385042465 >10 1.0957127889547285 13.086184388421731 >50 0.10898487932545829 4.50204268077335 >100 0.0570029280456775 5.849335356462177 >500 0.0020674637115012124 0.9654208349047259 >1k 8.860558763576625E-4 1.3685967665098442 >5k 2.953519587858875E-4 0.9363055320499309 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5274 0.9359876053518472 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4046 0.7180519247731464 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2660 0.4720756598854596 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1003 0.17800446874628417 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 972 0.17250283511604012 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 885 0.15706276654083898 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 855 0.15173860496318342 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 0.14694685954329342 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 692 0.12281066039125489 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 665 0.1180189149713649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.873602629425932E-5 0.0 18-19 0.0 0.0 0.0 1.7747205258851863E-4 0.0 20-21 0.0 0.0 0.0 2.6620807888277795E-4 0.0 22-23 0.0 0.0 0.0 5.324161577655559E-4 0.0 24-25 0.0 0.0 0.0 7.986242366483338E-4 0.0 26-27 0.0 0.0 0.0 9.760962892368524E-4 0.0 28-29 0.0 0.0 0.0 0.0023071366836507423 0.0 30-31 0.0 0.0 0.0 0.0044368013147129655 0.0 32-33 0.0 0.0 0.0 0.0070988821035407445 0.0 34-35 0.0 0.0 0.0 0.00922854673460297 0.0 36-37 0.0 0.0 0.0 0.014641444338552786 0.0 38-39 0.0 0.0 0.0 0.02316010286280168 0.0 40-41 0.0 0.0 0.0 0.032033705492227615 0.0 42-43 0.0 0.0 0.0 0.0438355969893641 0.0 44-45 0.0 0.0 0.0 0.05430644809208669 0.0 46-47 0.0 0.0 0.0 0.07844264724412522 0.0 48-49 0.0 0.0 0.0 0.10044918176510154 0.0 50-51 0.0 0.0 0.0 0.11553430623512562 0.0 52-53 0.0 0.0 0.0 0.14038039359751825 0.0 54-55 1.7747205258851863E-4 0.0 0.0 0.1781819407988727 0.0 56-57 1.7747205258851863E-4 0.0 0.0 0.23914359086302883 0.0 58-59 1.7747205258851863E-4 0.0 0.0 0.3097774677932592 0.0 60-61 1.7747205258851863E-4 0.0 0.0 0.39638382945645634 0.0 62-63 1.7747205258851863E-4 0.0 0.0 0.4830789271459477 0.0 64-65 1.7747205258851863E-4 0.0 0.0 0.5703064409932046 0.0 66-67 1.7747205258851863E-4 0.0 0.0 0.6759910483096674 0.0 68-69 1.7747205258851863E-4 0.0 0.0 0.8228491718266666 0.0 70-71 1.7747205258851863E-4 0.0 0.0 0.9166431516196987 0.0 72-73 1.7747205258851863E-4 0.0 0.0 1.0398974921424249 0.0 74-75 1.7747205258851863E-4 0.0 0.0 1.17584108442523 0.0 76-77 1.7747205258851863E-4 0.0 0.0 1.32083575139005 0.0 78-79 1.7747205258851863E-4 0.0 0.0 1.4901440895594966 0.0 80-81 1.7747205258851863E-4 0.0 0.0 1.7349667861053581 0.0 82-83 1.7747205258851863E-4 0.0 0.0 2.0009973929355476 0.0 84-85 2.6620807888277795E-4 0.0 0.0 2.3177850068060533 0.0 86-87 3.5494410517703727E-4 0.0 0.0 2.71647597294616 0.0 88-89 3.5494410517703727E-4 0.0 0.0 3.1850909278061437 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 370 0.0 39.825905 2 GAACCGT 50 0.001615056 38.000286 6 GTACCTG 445 0.0 33.1284 1 GTACACG 75 3.0555448E-4 31.703524 1 TATAACG 65 0.0058210935 29.25178 2 GGTATCA 4020 0.0 28.746103 1 TAACGCA 70 0.008358643 27.162367 4 ACCTGGG 530 0.0 26.903727 3 GGATAGG 125 7.3157207E-6 26.630957 1 GTATCAA 5645 0.0 26.536608 1 GTACATG 4555 0.0 25.996193 1 TACATGG 4615 0.0 25.543808 2 CATGGGG 1375 0.0 24.890604 4 ACATGGG 4570 0.0 24.857002 3 GGTCTAT 115 1.2705417E-4 24.811453 1 GTATAGG 255 1.8189894E-11 24.24387 1 ATGGGAT 1110 0.0 23.124718 5 GAGTACT 3580 0.0 22.819372 12-13 TAGGACA 315 0.0 22.635305 4 TATCAAC 6735 0.0 22.020271 2 >>END_MODULE