FastQCFastQC Report
Thu 26 May 2016
SRR937722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563469
Sequences flagged as poor quality0
Sequence length101
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59091.0486823587455567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52310.928356307090541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36230.642981246528203No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12240.21722579236834677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.2143862395269305No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11300.20054341942502604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.18741048753347567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7590.13470128791468564No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.12795734991632193No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6810.12085846781278119No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6240.11074256081523562No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT5670.10062665381769006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG4150.041.2085152
GTACCTG5300.033.1721841
CACCGTA600.003950955331.6638897
ACCTGGG5950.028.7420733
GCACTCC856.4063846E-427.938726
GTATCAA65900.027.3276331
GGTATCA47350.027.1955281
CCTCCGT700.00839159727.1404727
GTAATAC1457.869421E-726.2092483
TCTAGAC1452.329995E-522.9330923
GTACATG48000.022.8675141
TACATGG48350.022.401182
GTATGGT1703.1345608E-622.3608991
GAGTACT44850.022.18590212-13
TCAACGC81400.022.0013584
CATGGGG13400.021.9795844
TATCAAC81350.021.9564882
ATCAACG81650.021.9339963
CAACGCA81850.021.8803985
AGTACTT47200.021.68506612-13
Produced by FastQC (version 0.11.5)