##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937722_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 563469 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24603838010609 33.0 31.0 34.0 31.0 34.0 2 32.451930807196135 34.0 31.0 34.0 31.0 34.0 3 32.505323274217396 34.0 31.0 34.0 31.0 34.0 4 36.12810820116102 37.0 35.0 37.0 35.0 37.0 5 36.05442003020574 37.0 35.0 37.0 35.0 37.0 6 36.120196497056625 37.0 35.0 37.0 35.0 37.0 7 36.074737030786075 37.0 35.0 37.0 35.0 37.0 8 36.059021880529365 37.0 35.0 37.0 35.0 37.0 9 37.731044653743155 39.0 37.0 39.0 35.0 39.0 10-11 37.66479078707081 39.0 37.0 39.0 35.0 39.0 12-13 37.563137457428894 39.0 37.0 39.0 35.0 39.0 14-15 38.70219036007305 40.0 38.0 41.0 34.5 41.0 16-17 38.668928547976904 40.0 38.0 41.0 34.5 41.0 18-19 38.75482502142975 40.0 38.0 41.0 35.0 41.0 20-21 38.696715347250695 40.0 38.0 41.0 34.0 41.0 22-23 38.54220462882608 40.0 38.0 41.0 34.0 41.0 24-25 38.60100111984865 40.0 38.0 41.0 34.0 41.0 26-27 38.443531054947115 40.0 38.0 41.0 34.0 41.0 28-29 38.2956054370338 40.0 38.0 41.0 34.0 41.0 30-31 38.26205612021247 40.0 37.5 41.0 34.0 41.0 32-33 38.09348251634074 40.0 37.0 41.0 34.0 41.0 34-35 37.93745441186649 40.0 37.0 41.0 33.5 41.0 36-37 37.73862359774894 40.0 37.0 41.0 33.0 41.0 38-39 37.57114055964037 40.0 36.0 41.0 33.0 41.0 40-41 37.36599085309041 40.0 36.0 41.0 33.0 41.0 42-43 37.097021309069355 40.0 36.0 41.0 32.5 41.0 44-45 36.6524183229246 40.0 36.0 41.0 31.0 41.0 46-47 36.097827919548365 39.5 35.5 41.0 30.0 41.0 48-49 35.59655367021078 39.0 35.0 41.0 27.5 41.0 50-51 35.29269667009188 39.0 35.0 41.0 26.0 41.0 52-53 35.0399746924853 39.0 35.0 40.0 24.5 41.0 54-55 34.83872138484992 39.0 35.0 40.0 24.0 41.0 56-57 34.604289676983115 38.5 35.0 40.0 23.5 41.0 58-59 34.348387400194156 38.0 34.0 40.0 23.0 41.0 60-61 34.015558974850435 38.0 34.0 40.0 22.0 41.0 62-63 33.6676596228009 38.0 34.0 40.0 20.5 41.0 64-65 33.290724068227355 37.0 33.0 40.0 18.5 41.0 66-67 32.86938234401538 36.5 33.0 39.0 16.5 41.0 68-69 32.428535553863654 36.0 32.0 39.0 12.5 40.0 70-71 32.02933613029288 35.5 32.0 39.0 8.0 40.0 72-73 31.526206410645482 35.0 31.0 38.0 7.0 40.0 74-75 31.008163714419073 35.0 31.0 37.0 4.5 39.0 76-77 29.75916687519633 33.5 29.5 36.0 2.0 38.5 78-79 30.34028580099349 34.0 30.0 36.0 2.0 39.0 80-81 30.133264651648982 34.0 30.0 36.0 2.0 38.0 82-83 29.771174634274466 34.0 30.0 35.5 2.0 37.0 84-85 29.35257219119419 34.0 29.5 35.0 2.0 37.0 86-87 28.931722951928144 34.0 29.0 35.0 2.0 36.0 88-89 28.54208217310979 34.0 29.0 35.0 2.0 36.0 90-91 28.00033187273834 34.0 28.5 35.0 2.0 35.5 92-93 27.526931383980305 33.0 27.0 35.0 2.0 35.0 94-95 27.044653743151798 33.0 26.0 35.0 2.0 35.0 96-97 26.490034944247157 33.0 25.0 35.0 2.0 35.0 98-99 25.636417442663216 32.5 23.5 34.0 2.0 35.0 100-101 23.035196257469355 29.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34830012594974846 1101 2 0.3025626132687407 1101 3 0.12008130936666106 1101 4 0.10551510006173714 1101 5 0.08832526228389526 1101 6 0.0037275292022158624 1101 7 0.11451504041569649 1101 8 0.16521531340625017 1101 9 0.28363685974904485 1101 10-11 0.31202095154397114 1101 12-13 0.30468746811348524 1101 14-15 0.3767684811486589 1101 16-17 0.2147413369651403 1101 18-19 0.1675562299048039 1101 20-21 0.3161336594429187 1101 22-23 0.34924449654211287 1101 24-25 0.28771993413354124 1101 26-27 0.3327927564125517 1101 28-29 0.3430545361398174 1101 30-31 0.349191895754025 1101 32-33 0.3687295797119816 1101 34-35 0.37683738426505187 1101 36-37 0.35322833965781797 1101 38-39 0.46520196699845684 1101 40-41 0.48548120113789395 1101 42-43 0.4991076753729544 1101 44-45 0.6093231938274712 1101 46-47 0.5535832207022722 1101 48-49 0.6763411650294557 1101 50-51 0.6762230873269033 1101 52-53 0.693150532766083 1101 54-55 0.6803498445428033 1101 56-57 0.7606255580489787 1101 58-59 0.7768648093654775 1101 60-61 0.8847349901999664 1101 62-63 0.9465097123876021 1101 64-65 1.1190895682570599 1101 66-67 0.8519789980251815 1101 68-69 0.8892169766224214 1101 70-71 0.9513682045363048 1101 72-73 0.8766016300083344 1101 74-75 0.684559792716751 1101 76-77 0.5285445587457183 1101 78-79 0.8187136451143218 1101 80-81 0.830555602683777 1101 82-83 0.9987652639351197 1101 84-85 0.9816220560575459 1101 86-87 0.9185485373179816 1101 88-89 1.194217050267131 1101 90-91 1.0583741767240227 1101 92-93 1.0557046266378727 1101 94-95 1.1005701031527906 1101 96-97 1.0803785048488628 1101 98-99 1.0573078226190802 1101 100-101 1.5939677971504231 1102 1 0.19387057810537556 1102 2 0.10829670895748933 1102 3 0.027800157947098114 1102 4 0.06349025779044126 1102 5 0.015763485207138217 1102 6 -0.045749994457089826 1102 7 0.044628079322109215 1102 8 0.025309950630223454 1102 9 -0.036154806917622295 1102 10-11 -0.04539065466002512 1102 12-13 -0.0041354814133285345 1102 14-15 -0.06482096417204986 1102 16-17 0.1519110924856406 1102 18-19 0.13303007322762284 1102 20-21 0.1529107362042268 1102 22-23 0.10550271157892155 1102 24-25 -0.02993253694952358 1102 26-27 0.13384592413074614 1102 28-29 0.0918962159505412 1102 30-31 0.12534692992857543 1102 32-33 0.04323503449641919 1102 34-35 0.05135926912310396 1102 36-37 0.09193102104583062 1102 38-39 0.17324120201422488 1102 40-41 0.17435639829877658 1102 42-43 0.17262568273363144 1102 44-45 -0.11406501595170226 1102 46-47 -0.08912182661634205 1102 48-49 -0.08163563570660415 1102 50-51 -0.2190647675626849 1102 52-53 -0.008226307402161126 1102 54-55 -0.08501391728685093 1102 56-57 -0.2531477726555451 1102 58-59 -0.09531907601832756 1102 60-61 -0.1665629856149664 1102 62-63 0.08916054046331823 1102 64-65 0.28429724438745296 1102 66-67 0.302199161011508 1102 68-69 0.15864060964029392 1102 70-71 0.21387806258046638 1102 72-73 0.28932507900939086 1102 74-75 0.2413109099617543 1102 76-77 0.14086711395076534 1102 78-79 0.29739562460959235 1102 80-81 0.23552241393509377 1102 82-83 0.08717384700556963 1102 84-85 -0.0636625128173236 1102 86-87 0.014748261292744758 1102 88-89 0.2145410513186583 1102 90-91 0.2694332592687161 1102 92-93 0.2380587275842423 1102 94-95 0.22467959316330166 1102 96-97 0.23509393597399608 1102 98-99 0.19522186047396772 1102 100-101 0.46562920619353676 1103 1 0.13351074866037038 1103 2 0.15722149605262103 1103 3 0.10811931302966116 1103 4 0.04320969713133138 1103 5 0.13175474946338284 1103 6 0.11500271235239978 1103 7 0.06016522356588183 1103 8 0.12766952586046187 1103 9 0.0828928121299981 1103 10-11 0.01563244421795673 1103 12-13 0.1386274864285042 1103 14-15 0.30264416796185145 1103 16-17 0.27797966114095374 1103 18-19 0.02674807972165638 1103 20-21 0.0938809121901798 1103 22-23 0.2984603848205012 1103 24-25 0.2596521685657791 1103 26-27 0.1528877655700569 1103 28-29 0.20775806178083656 1103 30-31 0.17387710637672882 1103 32-33 0.3046556328255363 1103 34-35 0.0062043714445465525 1103 36-37 0.18324207592155517 1103 38-39 0.2631586977676861 1103 40-41 0.1410386645078603 1103 42-43 0.3017061185963996 1103 44-45 0.3999081297249134 1103 46-47 0.4028964075154491 1103 48-49 0.24584093609173152 1103 50-51 0.1991855415393573 1103 52-53 0.08027278551333694 1103 54-55 0.14188258263803988 1103 56-57 -0.03319884671292783 1103 58-59 0.03463724526291401 1103 60-61 0.10271804880802193 1103 62-63 0.1423029816183785 1103 64-65 0.20237711404460157 1103 66-67 0.19727432769551 1103 68-69 0.26871560299604624 1103 70-71 0.30495107999417925 1103 72-73 0.08887269227940209 1103 74-75 0.14817586414531903 1103 76-77 0.04898869793985838 1103 78-79 0.33139107185424876 1103 80-81 0.4103690184346931 1103 82-83 0.23342071265306785 1103 84-85 0.11162434543483357 1103 86-87 0.2428216600286035 1103 88-89 0.287245896420977 1103 90-91 0.23747788551523286 1103 92-93 0.175599315282561 1103 94-95 0.19616305187073735 1103 96-97 0.16257859642395545 1103 98-99 0.35343877500003273 1103 100-101 0.655872559055183 1104 1 0.17181568696531002 1104 2 0.21710755593868214 1104 3 0.02673313164348201 1104 4 0.03917859310023175 1104 5 0.08233120003983174 1104 6 -0.008114710765021016 1104 7 0.021077284477939884 1104 8 0.14756584575678033 1104 9 0.18047090970809876 1104 10-11 0.2365129150984302 1104 12-13 0.20172114952216447 1104 14-15 0.39629781161549715 1104 16-17 0.3697743529356501 1104 18-19 0.10424490721848656 1104 20-21 0.1243031926124587 1104 22-23 0.24167257803269848 1104 24-25 0.12354098245459255 1104 26-27 0.1778006904829823 1104 28-29 0.04346019748297181 1104 30-31 0.16689894939857908 1104 32-33 0.17836435653426008 1104 34-35 0.35542947066964103 1104 36-37 0.39095868363816777 1104 38-39 0.4154361500451387 1104 40-41 0.3644827879519781 1104 42-43 0.4792852961755827 1104 44-45 0.7701122499290278 1104 46-47 0.617803122422167 1104 48-49 0.6486934889442821 1104 50-51 0.9005422928961053 1104 52-53 0.9360021912427428 1104 54-55 1.0054662962217549 1104 56-57 0.9179594544453735 1104 58-59 1.0243745350002058 1104 60-61 0.9193352154251855 1104 62-63 1.03400587332127 1104 64-65 1.1774331391006356 1104 66-67 1.0766949071160852 1104 68-69 0.9058554901359344 1104 70-71 1.1831377581808589 1104 72-73 1.1378628912696023 1104 74-75 1.0491060150754699 1104 76-77 0.7386233875745454 1104 78-79 1.0322105226737008 1104 80-81 0.800801558867235 1104 82-83 0.9621392913716456 1104 84-85 0.9842044680149549 1104 86-87 1.2133133305202755 1104 88-89 1.073401632576715 1104 90-91 1.0296694777068254 1104 92-93 1.000918590601838 1104 94-95 1.2704590261667121 1104 96-97 1.419938803784163 1104 98-99 1.4196900912513506 1104 100-101 1.7102249634075868 1105 1 -0.0095318242577207 1105 2 -0.12251950982945914 1105 3 -0.04035696864163896 1105 4 0.04268228540060193 1105 5 -0.1064620821144473 1105 6 0.005019887431821246 1105 7 -0.04248703427310119 1105 8 -0.08464637400730624 1105 9 -0.05551856901997354 1105 10-11 -0.06487004084884518 1105 12-13 0.031031693563001284 1105 14-15 -0.009203596997949148 1105 16-17 0.005256119122812208 1105 18-19 0.0963641483003812 1105 20-21 -0.0011899920093583205 1105 22-23 -0.10399504356853129 1105 24-25 -0.10043705065206865 1105 26-27 -0.23690598148510134 1105 28-29 -0.2658774313567278 1105 30-31 -0.12395168999977813 1105 32-33 0.2379874462403393 1105 34-35 -0.06914938959386774 1105 36-37 2.978417325110172E-4 1105 38-39 0.08266609217135112 1105 40-41 -0.04148759208478481 1105 42-43 -0.1273402845163929 1105 44-45 -0.14905984559161567 1105 46-47 -0.21026532701557699 1105 48-49 -0.07771096152655588 1105 50-51 0.08950974016231328 1105 52-53 0.16791280800813269 1105 54-55 0.1270930153588452 1105 56-57 0.16160455182491518 1105 58-59 0.27833870286478657 1105 60-61 -0.00920951187472241 1105 62-63 0.0459629454443089 1105 64-65 -0.028265203527169547 1105 66-67 0.08046706856459451 1105 68-69 -0.1840757273886453 1105 70-71 -0.10598397388693215 1105 72-73 0.06539831050626432 1105 74-75 -0.04427622042294743 1105 76-77 -0.13342812451845987 1105 78-79 0.12111034290132139 1105 80-81 0.08216996990369552 1105 82-83 0.40634868371381927 1105 84-85 -0.023534410055873423 1105 86-87 0.08954395571355533 1105 88-89 0.06993022454098963 1105 90-91 -0.12435620017777183 1105 92-93 -0.11000249646032856 1105 94-95 -0.4551136796964528 1105 96-97 -0.24206703457022272 1105 98-99 -0.06056043464232985 1105 100-101 0.26042699493742205 1106 1 -0.029687173168966297 1106 2 -0.09984958216931261 1106 3 -0.07340786896408247 1106 4 -0.12208177655827512 1106 5 -0.07496854279645504 1106 6 -0.1885725615183631 1106 7 -0.060983922777666066 1106 8 0.018533094640048375 1106 9 -0.11806253112705178 1106 10-11 -0.2416864421833509 1106 12-13 -0.3211857174748971 1106 14-15 -0.1841690188513354 1106 16-17 -0.0754341101577225 1106 18-19 -0.10987540565766096 1106 20-21 -0.1954137744606541 1106 22-23 -0.16356087541226572 1106 24-25 -0.025350309516014136 1106 26-27 0.04440418072566388 1106 28-29 0.12447058720253779 1106 30-31 -0.0458954228048114 1106 32-33 0.21115846125682225 1106 34-35 0.12724157685188686 1106 36-37 -0.37487236018665726 1106 38-39 -0.12414241480558985 1106 40-41 -0.15869729160446155 1106 42-43 -0.0231917673434765 1106 44-45 0.15983754711933074 1106 46-47 0.4139836872652509 1106 48-49 0.4704499973259857 1106 50-51 0.34984607851404803 1106 52-53 0.3782253772450872 1106 54-55 0.3363171527335709 1106 56-57 0.08986269516665857 1106 58-59 -0.06919694542975208 1106 60-61 -0.04020830943715481 1106 62-63 0.16545168361621876 1106 64-65 0.4171131556080212 1106 66-67 0.14427584872502308 1106 68-69 0.3211911212569447 1106 70-71 0.4621785337224047 1106 72-73 0.561153211138457 1106 74-75 0.4647721760760035 1106 76-77 0.23936946802513503 1106 78-79 0.275309517327905 1106 80-81 0.2249965371057563 1106 82-83 0.2664808873151081 1106 84-85 0.047493297633486975 1106 86-87 0.3161711721028446 1106 88-89 -0.20253158892415968 1106 90-91 -0.0936305861064568 1106 92-93 0.19545644104948323 1106 94-95 0.36623722342235965 1106 96-97 0.5344301507793965 1106 98-99 0.33405419379585766 1106 100-101 0.5429309905148578 1107 1 0.14021189065562822 1107 2 0.2175748681707006 1107 3 0.006417093680383346 1107 4 0.0931989235911459 1107 5 0.05045761522507064 1107 6 0.11420547037869255 1107 7 0.24760327570981389 1107 8 0.21913688203370185 1107 9 0.21286480092551585 1107 10-11 0.1327623731125911 1107 12-13 0.10070462497623112 1107 14-15 0.08838000075650854 1107 16-17 0.059686189906308584 1107 18-19 0.13967632794401652 1107 20-21 0.24113365944292298 1107 22-23 0.15528003575780502 1107 24-25 0.07914150276099718 1107 26-27 0.3300047662164687 1107 28-29 0.2605484086888339 1107 30-31 0.24168576830305 1107 32-33 -0.005320665386058465 1107 34-35 -0.062347664754554444 1107 36-37 -0.33243342504805895 1107 38-39 -0.004865434962326276 1107 40-41 0.012962818784949093 1107 42-43 -0.17190948148978435 1107 44-45 -0.22912656107448726 1107 46-47 -0.8750932498859711 1107 48-49 -0.837966433009754 1107 50-51 -0.7903210303201575 1107 52-53 -0.6744352515476422 1107 54-55 -0.7040557927120972 1107 56-57 -0.7990251772451344 1107 58-59 -0.6718484259286441 1107 60-61 -0.4529488333294509 1107 62-63 -0.5828101405535691 1107 64-65 -0.3806653337037247 1107 66-67 -0.3549450215826653 1107 68-69 -0.34687370965209396 1107 70-71 -0.46168326605193144 1107 72-73 -0.5890846445014652 1107 74-75 -0.5291350602244265 1107 76-77 -0.5098132843915373 1107 78-79 -0.2845645411601865 1107 80-81 -0.22008777966916426 1107 82-83 -0.171179589006055 1107 84-85 0.009501958018326206 1107 86-87 -0.3240066097408416 1107 88-89 -0.08145696934071012 1107 90-91 -0.17621528406029086 1107 92-93 0.042836979579050194 1107 94-95 0.5366607894272981 1107 96-97 0.6847289950449422 1107 98-99 0.5265480186975147 1107 100-101 0.46651681675826495 1108 1 -0.06084603690906931 1108 2 0.03835091145268876 1108 3 -0.008783292273648158 1108 4 -0.06604502003199286 1108 5 -0.16444641317011843 1108 6 -0.05402913453533387 1108 7 -0.1582515090863552 1108 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-0.6018365984298875 2316 74-75 -0.8847911688217103 2316 76-77 -0.8955667466155894 2316 78-79 -0.37740013693695573 2316 80-81 -0.44617443810876267 2316 82-83 -0.6025932733609167 2316 84-85 -0.7465175127070225 2316 86-87 -0.4464390792321353 2316 88-89 -0.12953732036223897 2316 90-91 -0.11921542117807604 2316 92-93 -0.2684066787234336 2316 94-95 -0.015025759318074705 2316 96-97 -0.14706176321640285 2316 98-99 0.3124797340409948 2316 100-101 0.8528256060082278 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 8.0 5 13.0 6 23.0 7 69.0 8 137.0 9 299.0 10 517.0 11 939.0 12 1647.0 13 2478.0 14 3014.0 15 4411.0 16 6526.0 17 9973.0 18 8891.0 19 4466.0 20 3150.0 21 2873.0 22 3063.0 23 3271.0 24 3617.0 25 4248.0 26 5160.0 27 6374.0 28 7691.0 29 9546.0 30 12066.0 31 15072.0 32 19605.0 33 25649.0 34 36033.0 35 52107.0 36 78040.0 37 121818.0 38 100907.0 39 9764.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79371063823744 21.004359525990303 15.65044979799916 19.551480037773093 2 15.222132894622419 22.343376476789317 41.30324827097853 21.131242357609736 3 19.310201625998946 28.324007176969808 31.12522605502698 21.240565142004264 4 12.049997426655237 18.309436721452286 38.926365070660495 30.714200781231977 5 11.550314934350213 40.303430547566805 34.49170234975821 13.654552168324777 6 28.311229189183436 38.800537385375236 18.16532941475041 14.722904010690915 7 26.20073153980077 36.02948875625811 21.321137453879448 16.44864225006167 8 26.36382835612962 35.38437784509884 20.056471607133666 18.19532219163787 9 27.730363161061213 16.95443759993895 21.746892908039307 33.56830633096053 10-11 24.122800012068097 28.75632909707544 27.00130796902758 20.119562921828884 12-13 25.781080841245895 26.687283698642293 28.359126808057507 19.172508652054308 14-15 22.921048007964945 27.123674949287363 28.381508122008487 21.573768920739205 16-17 19.535147452654893 30.67302726503144 29.249080251087463 20.542745031226207 18-19 19.295648917686687 28.33199341933629 33.563248377461754 18.809109285515262 20-21 20.654729896409563 27.002993953527167 33.840016043473554 18.502260106589716 22-23 20.280285592081796 27.184933828605708 33.63577958368015 18.89900099563235 24-25 19.926828272717753 27.483499535910582 34.02724905895444 18.562423132417223 26-27 19.744404523056748 27.473141799408484 34.175291076608545 18.607162600926227 28-29 19.295799894225343 27.41125399760765 34.532852259779716 18.760093848387292 30-31 19.598682447481583 27.61509506290497 34.28547089547074 18.500751594142713 32-33 19.47862260390545 27.79034871483613 33.92768723745228 18.803341443806136 34-35 19.68120695193524 27.60613272424925 34.12450374377295 18.588156580042558 36-37 20.00429482367264 27.529464797531013 33.804610368982146 18.661630009814203 38-39 19.79596020008217 27.92285637972664 33.856196042902134 18.42498737728906 40-41 19.589699084858054 27.81186747346106 34.09079010301447 18.50764333866641 42-43 19.39191911661599 28.032195023243677 33.44418233551482 19.131703524625518 44-45 19.714571697821885 28.321700040286157 33.013883638674 18.94984462321796 46-47 20.090989921362134 28.43350743341692 32.21499319394678 19.26050945127416 48-49 20.12923514869496 28.83095609518891 31.684795436838588 19.355013319277546 50-51 20.454097740958243 29.042591517900718 31.17846767080354 19.3248430703375 52-53 20.2738748715546 29.388484548395745 31.16258392209687 19.175056657952787 54-55 20.068096026578214 29.11677483588272 31.22949088592274 19.585638251616327 56-57 20.483114421556465 29.75176984004444 30.450211103006552 19.31490463539254 58-59 20.424282436123374 29.099471310755337 30.9487300987277 19.527516154393588 60-61 20.518697568100464 29.248636570955988 31.06444187701542 19.168223983928133 62-63 20.528103586887656 29.047826943452083 31.38327041949069 19.040799050169575 64-65 20.312563779018898 28.981984811941743 31.226385125002444 19.47906628403692 66-67 20.436616743778274 29.363283516928174 30.583758822579416 19.61634091671414 68-69 20.182867527525964 29.373540289776884 30.878860911356103 19.564731271341053 70-71 20.233784142325614 29.568112503183407 30.69958657848664 19.498516776004337 72-73 20.450370827853884 29.301700714679956 30.409037586806022 19.83889087066014 74-75 20.325874182963037 29.67998239477238 30.255967941448418 19.73817548081616 76-77 20.258168594900518 29.34473768743267 30.804001639841765 19.593092077825045 78-79 20.200578913835542 29.146590140717592 30.67098633642667 19.981844609020193 80-81 20.218414855120688 29.73819322802142 30.235647391427033 19.807744525430856 82-83 20.517905837399717 29.65322386711426 29.984302388006988 19.84456790747904 84-85 20.186594447244563 29.273534610661123 30.1153747861458 20.424496155948518 86-87 20.023994221509966 29.467104667692457 30.253749540791063 20.255151570006515 88-89 20.402542109681278 28.8929825775686 30.492893131654093 20.211582181096034 90-91 19.98771766969406 29.086049083173737 30.863082190443563 20.063151056688632 92-93 20.062844192192127 29.361424208058466 30.56879229470927 20.006939305040135 94-95 19.733117527317386 29.44367835675077 30.3196804083277 20.503523707604145 96-97 20.20466077104508 29.165934594449737 29.963316526729955 20.666088107775227 98-99 19.935257046209216 29.147462370820236 30.064388003386227 20.852892579584317 100-101 19.975095919760225 29.19901342210996 30.241150664360273 20.584739993769542 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 684.0 1 469.0 2 404.5 3 509.0 4 364.0 5 328.5 6 620.5 7 1196.5 8 1792.0 9 2098.5 10 1828.0 11 1478.0 12 1508.5 13 1619.0 14 1726.0 15 1795.0 16 1802.0 17 1870.0 18 1989.0 19 2158.0 20 2323.0 21 2553.5 22 2902.0 23 3327.0 24 3750.5 25 4389.5 26 5476.0 27 6599.0 28 7189.5 29 7820.5 30 9130.0 31 10482.5 32 12367.0 33 14886.5 34 17409.5 35 19184.0 36 21672.5 37 23737.0 38 23383.5 39 22775.0 40 21766.0 41 20405.0 42 19265.0 43 19209.5 44 19892.0 45 20056.5 46 19010.5 47 18110.5 48 20585.5 49 19999.5 50 16765.5 51 14780.0 52 12298.5 53 11136.0 54 10560.5 55 9497.0 56 8066.5 57 6886.0 58 5978.0 59 5255.0 60 4374.0 61 3429.0 62 2624.5 63 1907.0 64 1275.5 65 853.5 66 633.5 67 455.0 68 303.0 69 222.0 70 172.0 71 116.0 72 79.0 73 73.0 74 57.5 75 40.5 76 29.5 77 16.0 78 10.0 79 5.0 80 3.5 81 4.0 82 3.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018634565521794454 2 0.0 3 0.0 4 0.0 5 0.0314125533081678 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033719689991818535 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001419776420708149 24-25 0.0 26-27 8.873602629425932E-5 28-29 0.0012423043681196303 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.873602629425932E-5 40-41 7.986242366483338E-4 42-43 0.015972484732966676 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7747205258851863E-4 70-71 8.873602629425932E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0013310403944138897 84-85 1.7747205258851863E-4 86-87 0.0 88-89 0.0 90-91 0.010293379050134081 92-93 0.0021296646310622236 94-95 0.0 96-97 0.0 98-99 0.0017747205258851861 100-101 0.01907824565326575 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 563469.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.880677698514724 #Duplication Level Percentage of deduplicated Percentage of total 1 84.67043420773373 49.007821129711324 2 9.096883205272462 10.530675297308141 3 2.3592226060836947 4.096602098453409 4 1.0060022425789623 2.3291236626678375 5 0.5674172714227055 1.6421248103894135 6 0.3573123213224835 1.2408887584904877 7 0.2638969095373905 1.0692172376597455 8 0.20267464419304634 0.9384756606559093 9 0.16333637322803904 0.8508617977730795 >10 1.1232715597218972 12.897302446098942 >50 0.12279323623636061 4.9377511611195715 >100 0.06277459471191939 6.336712530802048 >500 0.001837305211080559 0.6873397626552439 >1k 0.0015310876759004658 1.4606413058385417 >5k 6.124350703601864E-4 1.9744623403763324 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5909 1.0486823587455567 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5231 0.928356307090541 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3623 0.642981246528203 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1224 0.21722579236834677 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1208 0.2143862395269305 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1130 0.20054341942502604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1056 0.18741048753347567 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 759 0.13470128791468564 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 721 0.12795734991632193 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 681 0.12085846781278119 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 624 0.11074256081523562 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 567 0.10062665381769006 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.873602629425932E-5 0.0 18-19 0.0 0.0 0.0 1.7747205258851863E-4 0.0 20-21 0.0 0.0 0.0 2.6620807888277795E-4 0.0 22-23 0.0 0.0 0.0 6.211521840598152E-4 0.0 24-25 0.0 0.0 0.0 9.760962892368524E-4 0.0 26-27 0.0 0.0 0.0 0.0011535683418253712 0.0 28-29 0.0 0.0 0.0 0.002839552841416298 0.0 30-31 0.0 0.0 0.0 0.0050579534987727805 0.0 32-33 0.0 0.0 0.0 0.007897506340189079 0.0 34-35 0.0 0.0 0.0 0.009938434944957043 0.0 36-37 0.0 0.0 0.0 0.015351332548906861 0.0 38-39 0.0 0.0 0.0 0.023958727099450017 0.0 40-41 0.0 0.0 0.0 0.032832329728875946 0.0 42-43 0.0 0.0 0.0 0.04463422122601243 0.0 44-45 0.0 0.0 0.0 0.05528254438132355 0.0 46-47 0.0 0.0 0.0 0.07862011929671375 0.0 48-49 0.0 0.0 0.0 0.10133654202804412 0.0 50-51 0.0 0.0 0.0 0.11659913855065673 0.0 52-53 0.0 0.0 0.0 0.1410902818078723 0.0 54-55 0.0 0.0 0.0 0.178980565035521 0.0 56-57 0.0 0.0 0.0 0.24038589523114848 0.0 58-59 0.0 0.0 0.0 0.3107535640824961 0.0 60-61 0.0 0.0 0.0 0.3968275095879277 0.0 62-63 0.0 0.0 0.0 0.48316766317224197 0.0 64-65 0.0 0.0 0.0 0.5707501211246759 0.0 66-67 0.0 0.0 0.0 0.6778545048618468 0.0 68-69 0.0 0.0 0.0 0.8258661967206714 0.0 70-71 0.0 0.0 0.0 0.9197489125399978 0.0 72-73 0.0 0.0 0.0 1.044068085378255 0.0 74-75 0.0 0.0 0.0 1.1823188143447112 0.0 76-77 0.0 0.0 0.0 1.3291769378617102 0.0 78-79 0.0 0.0 0.0 1.499106428215217 0.0 80-81 1.7747205258851863E-4 0.0 0.0 1.7461475254184347 0.0 82-83 1.7747205258851863E-4 0.0 0.0 2.013509172643038 0.0 84-85 1.7747205258851863E-4 0.0 0.0 2.333491283460137 0.0 86-87 1.7747205258851863E-4 0.0 0.0 2.736530314888663 0.0 88-89 1.7747205258851863E-4 0.0 0.0 3.2102919592737136 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 415 0.0 41.208515 2 GTACCTG 530 0.0 33.172184 1 CACCGTA 60 0.0039509553 31.663889 7 ACCTGGG 595 0.0 28.742073 3 GCACTCC 85 6.4063846E-4 27.93872 6 GTATCAA 6590 0.0 27.327633 1 GGTATCA 4735 0.0 27.195528 1 CCTCCGT 70 0.008391597 27.140472 7 GTAATAC 145 7.869421E-7 26.209248 3 TCTAGAC 145 2.329995E-5 22.933092 3 GTACATG 4800 0.0 22.867514 1 TACATGG 4835 0.0 22.40118 2 GTATGGT 170 3.1345608E-6 22.360899 1 GAGTACT 4485 0.0 22.185902 12-13 TCAACGC 8140 0.0 22.001358 4 CATGGGG 1340 0.0 21.979584 4 TATCAAC 8135 0.0 21.956488 2 ATCAACG 8165 0.0 21.933996 3 CAACGCA 8185 0.0 21.880398 5 AGTACTT 4720 0.0 21.685066 12-13 >>END_MODULE