FastQCFastQC Report
Thu 26 May 2016
SRR937721_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937721_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508589
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48430.9522423803896664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.7754788247484707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.5063027316752821No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9460.18600480938439487No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.18266222824323766No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8190.1610337620357499No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7690.15120264103234635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7030.13822556130785368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6540.12859106272451823No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT5930.11659709510036592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG250.00501980756.9995889
GGCCGAT250.00501980756.9995887
TACCTGG4150.048.1003842
ACCTGGG5700.035.013553
GTACCTG5950.033.5589371
GGGCCGA753.0761742E-431.6664356
GTATAGG1452.374145E-829.508721
GGTATCA40250.029.4109671
GTGTAGG2151.8189894E-1228.7462221
GTAGGGT1205.3577496E-627.7190593
CTGGTCG1554.577123E-827.5804469
GTACACG700.008351291527.1667581
GTAGGTA700.00836335627.158722
GTATCAA56000.027.081861
CATGGGG12150.026.9910764
GTACATG42250.025.5431821
GTATAGT1501.050068E-625.3556391
TAGGGTC1501.0527801E-625.3481374
TACATGG42800.024.8743422
CATGGGC4900.024.2488574
Produced by FastQC (version 0.11.5)