##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937721_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 508589 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.847006128720835 33.0 31.0 34.0 30.0 34.0 2 31.969124381376712 33.0 31.0 34.0 30.0 34.0 3 31.964279604946235 34.0 31.0 34.0 30.0 34.0 4 35.56054495869946 37.0 35.0 37.0 33.0 37.0 5 35.5678612789502 37.0 35.0 37.0 33.0 37.0 6 35.64405246672657 37.0 35.0 37.0 33.0 37.0 7 35.59488703058855 37.0 35.0 37.0 33.0 37.0 8 35.5912652456109 37.0 35.0 37.0 33.0 37.0 9 37.22172717066236 39.0 37.0 39.0 34.0 39.0 10-11 37.11476752348163 39.0 37.0 39.0 34.0 39.0 12-13 36.99791285301097 39.0 37.0 39.0 33.0 39.0 14-15 38.129118993922404 40.0 38.0 41.0 33.0 41.0 16-17 38.07034265389145 40.0 38.0 41.0 33.0 41.0 18-19 38.10290922532732 40.0 38.0 41.0 33.5 41.0 20-21 38.066633371936874 40.0 38.0 41.0 33.0 41.0 22-23 37.8810090269353 40.0 38.0 41.0 33.0 41.0 24-25 37.72648838256431 40.0 37.0 41.0 33.0 41.0 26-27 37.6473183651239 40.0 37.0 41.0 32.5 41.0 28-29 37.66121957022271 40.0 37.0 41.0 33.0 41.0 30-31 37.44389772488198 40.0 37.0 41.0 33.0 41.0 32-33 37.15650259836528 39.0 36.0 41.0 32.0 41.0 34-35 37.13417120700605 39.5 36.0 41.0 32.0 41.0 36-37 37.02430646356881 40.0 36.0 41.0 31.5 41.0 38-39 36.86917923903191 40.0 36.0 41.0 31.0 41.0 40-41 36.6155402495925 39.5 35.0 41.0 30.5 41.0 42-43 36.32914986364236 39.0 35.0 41.0 30.0 41.0 44-45 35.991869662930185 39.0 35.0 41.0 29.5 41.0 46-47 35.46834084103274 39.0 35.0 41.0 26.5 41.0 48-49 34.983640031538236 39.0 35.0 41.0 24.0 41.0 50-51 34.024613194544116 38.0 34.0 39.5 22.5 40.5 52-53 34.11957592476439 38.0 34.5 39.5 22.5 40.5 54-55 34.49037336631347 39.0 35.0 40.0 22.0 41.0 56-57 34.31250872511989 38.5 34.5 40.0 20.5 41.0 58-59 34.100112271401855 38.0 34.0 40.0 19.0 41.0 60-61 33.81162589045378 38.0 34.0 40.0 17.5 41.0 62-63 33.46654174588912 38.0 33.5 40.0 13.5 41.0 64-65 33.12119511039366 37.5 33.0 40.0 9.0 41.0 66-67 32.72664961294876 37.0 32.5 39.5 7.5 41.0 68-69 32.25422394113912 36.0 32.0 39.0 4.5 41.0 70-71 31.845680893609575 36.0 31.5 39.0 2.0 40.0 72-73 31.384928694879363 35.0 31.0 38.5 2.0 40.0 74-75 30.917720398986212 35.0 31.0 37.5 2.0 39.5 76-77 30.427575114680028 35.0 30.0 37.0 2.0 39.0 78-79 29.907785068100175 34.5 29.5 36.5 2.0 39.0 80-81 29.37327685026613 34.0 29.0 36.0 2.0 38.5 82-83 28.843110055467186 34.0 29.0 35.5 2.0 37.0 84-85 28.3714620253289 34.0 28.0 35.0 2.0 37.0 86-87 27.918903082842924 34.0 27.0 35.0 2.0 36.0 88-89 27.43717815367615 34.0 26.0 35.0 2.0 36.0 90-91 26.954353122069094 33.0 25.5 35.0 2.0 35.5 92-93 26.417519844117745 33.0 25.0 35.0 2.0 35.0 94-95 25.819163410927096 32.5 23.5 35.0 2.0 35.0 96-97 25.128054283517734 32.0 19.0 34.5 2.0 35.0 98-99 24.242482633324748 32.0 4.5 34.0 2.0 35.0 100-101 21.940350656423952 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2097184434918269 1101 2 -0.10946810167942189 1101 3 -0.27640226641438304 1101 4 0.08849923475258237 1101 5 -0.18998141181319284 1101 6 -0.10955230145578554 1101 7 -0.09070766018490417 1101 8 -0.18354922023236497 1101 9 0.05118192483035955 1101 10-11 -0.08228241735965725 1101 12-13 -0.11067814639303464 1101 14-15 -0.06163204514145093 1101 16-17 0.23523764647947587 1101 18-19 -0.13536483495585827 1101 20-21 -0.12457580233567 1101 22-23 -0.1536885546042015 1101 24-25 0.057398878316014645 1101 26-27 0.017397310845574054 1101 28-29 -0.18053048465970534 1101 30-31 0.035331770314108724 1101 32-33 -0.06237739832808131 1101 34-35 0.010749941009727593 1101 36-37 0.17703704138782683 1101 38-39 0.19687790838585073 1101 40-41 -0.021360798308371898 1101 42-43 -0.007209260819955432 1101 44-45 0.20902631142277528 1101 46-47 0.41957142991309127 1101 48-49 0.46538120449037734 1101 50-51 0.32141931373909216 1101 52-53 0.34672723909437053 1101 54-55 0.18924084515054318 1101 56-57 0.2358877792624341 1101 58-59 0.35184866693993655 1101 60-61 0.3103327880186342 1101 62-63 0.231473114416886 1101 64-65 0.009315309360701463 1101 66-67 0.19814170322151625 1101 68-69 0.05893364722249572 1101 70-71 0.28470829251320495 1101 72-73 0.2703591587791756 1101 74-75 0.325646463195163 1101 76-77 0.6379746386792498 1101 78-79 0.6531370273871389 1101 80-81 0.4645178347977321 1101 82-83 0.5288466055897594 1101 84-85 0.8063533879152907 1101 86-87 0.7075363077611847 1101 88-89 0.5803853883653396 1101 90-91 0.6492905788387482 1101 92-93 0.5959917136270079 1101 94-95 0.4738995111066622 1101 96-97 0.945236362228929 1101 98-99 1.2323091822373868 1101 100-101 1.8519339442137657 1102 1 0.2814673532885479 1102 2 0.07733743008482818 1102 3 0.24507652506338573 1102 4 0.03414466434744412 1102 5 -0.04339270449955279 1102 6 0.04746270800363561 1102 7 0.01165205675565062 1102 8 0.08006987524119324 1102 9 0.045730860692799524 1102 10-11 0.01338147428901948 1102 12-13 0.11004998178309933 1102 14-15 0.29314962679736567 1102 16-17 0.28321614675165563 1102 18-19 0.036090545876341196 1102 20-21 0.07105725577983435 1102 22-23 0.21866061802932535 1102 24-25 0.183117851597423 1102 26-27 0.07868942621627895 1102 28-29 -0.016844392867135127 1102 30-31 0.2750187147871017 1102 32-33 0.2138168300913037 1102 34-35 0.4168351862124524 1102 36-37 0.3788577424890818 1102 38-39 0.27585362602682295 1102 40-41 0.35253005098417844 1102 42-43 0.36222024268402464 1102 44-45 0.4709153030901092 1102 46-47 0.7088051975805172 1102 48-49 1.0082055561296883 1102 50-51 0.9078179189975373 1102 52-53 0.6949025410536294 1102 54-55 0.7864920927666503 1102 56-57 0.8698556743218333 1102 58-59 0.876504073657081 1102 60-61 0.8481533520819653 1102 62-63 1.04821534227289 1102 64-65 0.9947245769310555 1102 66-67 1.0730622402579186 1102 68-69 1.0919426574660847 1102 70-71 1.1055083656000981 1102 72-73 1.1621562969705046 1102 74-75 1.1517510459717784 1102 76-77 1.2066744152313014 1102 78-79 1.1984927740344666 1102 80-81 1.3772284830251849 1102 82-83 1.4429181165470872 1102 84-85 1.3279749272318533 1102 86-87 1.3650081103047604 1102 88-89 1.4595274548211172 1102 90-91 1.3409266974679106 1102 92-93 1.3872258543421125 1102 94-95 1.739410491270423 1102 96-97 2.120662036284223 1102 98-99 2.258896443039589 1102 100-101 2.4100208947773325 1103 1 -0.044151886836161225 1103 2 -0.015821236992952237 1103 3 -0.039741100297771226 1103 4 -0.13028514368525634 1103 5 -0.03541595526753838 1103 6 0.028098963670721844 1103 7 0.08737264784590337 1103 8 -0.017432608571198216 1103 9 -0.027888482210343568 1103 10-11 -0.05237317643557304 1103 12-13 -0.013318410419437043 1103 14-15 0.005749693032363723 1103 16-17 0.0618328059954365 1103 18-19 -0.058563404812844055 1103 20-21 -0.16768636339176624 1103 22-23 -0.056122548003543216 1103 24-25 0.023432981726358548 1103 26-27 -0.16109553843934776 1103 28-29 -0.23127305891713235 1103 30-31 0.02226796393346575 1103 32-33 -0.07557871846009334 1103 34-35 0.11546666268189654 1103 36-37 0.006726260309726229 1103 38-39 0.1343091515101662 1103 40-41 0.3115599937708353 1103 42-43 0.30907236734285704 1103 44-45 0.3043920980014363 1103 46-47 0.446661638933989 1103 48-49 0.7452904454144615 1103 50-51 0.6810865304607674 1103 52-53 0.3577970960800698 1103 54-55 0.627565927658793 1103 56-57 0.7921309035748649 1103 58-59 0.675074739547199 1103 60-61 0.7910121059504363 1103 62-63 0.7386238294883967 1103 64-65 0.7905309309228628 1103 66-67 0.7288072697781658 1103 68-69 0.5929683006878435 1103 70-71 0.6603271043943941 1103 72-73 0.9397073435976573 1103 74-75 1.0869913476836075 1103 76-77 1.0441902602414075 1103 78-79 0.9500979734817498 1103 80-81 0.9421030933235883 1103 82-83 0.9766601369428969 1103 84-85 1.3272554781243144 1103 86-87 1.2094615002804368 1103 88-89 0.9453468845149509 1103 90-91 1.1707427240532695 1103 92-93 1.3319653109867424 1103 94-95 1.3278599071462658 1103 96-97 1.4699888374764534 1103 98-99 1.2753509864178056 1103 100-101 2.03386463728307 1104 1 0.1262722984395097 1104 2 0.14977210805583496 1104 3 0.15575523505004796 1104 4 0.18383733999168328 1104 5 0.2219597823062216 1104 6 0.1903348301145158 1104 7 0.262384791917448 1104 8 0.21947679665503728 1104 9 0.41046070985171923 1104 10-11 0.3541969448622737 1104 12-13 0.47608052947455093 1104 14-15 0.5370992565601469 1104 16-17 0.44468001928819234 1104 18-19 0.5233581802027985 1104 20-21 0.4315155210823889 1104 22-23 0.415732673232867 1104 24-25 0.3860817466028479 1104 26-27 0.697679624791256 1104 28-29 0.5036522193249766 1104 30-31 0.5463828754246194 1104 32-33 0.7257228402672098 1104 34-35 0.6236612321388435 1104 36-37 0.673579552781824 1104 38-39 0.8891042738795463 1104 40-41 0.8344176366779905 1104 42-43 0.8454260027063967 1104 44-45 0.8826150988729538 1104 46-47 1.3190899531939877 1104 48-49 1.064769714769973 1104 50-51 1.1800408901824468 1104 52-53 1.0273595880435522 1104 54-55 1.2314807834300865 1104 56-57 1.3748088142230657 1104 58-59 1.2830060683943643 1104 60-61 1.5848030921919118 1104 62-63 1.4473375580041008 1104 64-65 1.2686626726684622 1104 66-67 1.5222026807478386 1104 68-69 1.58496482176853 1104 70-71 1.4622617621458822 1104 72-73 1.4743938479623857 1104 74-75 1.6523577057479883 1104 76-77 1.5001179958721131 1104 78-79 1.517730629604504 1104 80-81 1.7094208965324782 1104 82-83 1.703449780192937 1104 84-85 1.5440057245775307 1104 86-87 1.901312828270374 1104 88-89 1.67228029214143 1104 90-91 1.4588858240775586 1104 92-93 1.2099888276241195 1104 94-95 1.5283478130797192 1104 96-97 1.9101685697353936 1104 98-99 1.5871518093572377 1104 100-101 2.1914536104661018 1105 1 0.09497409472751528 1105 2 -0.15080758045488452 1105 3 -0.09630477390651038 1105 4 -0.0534244403735471 1105 5 0.022760896900436478 1105 6 0.03935707224020035 1105 7 0.06017053108249826 1105 8 -0.12228067958519517 1105 9 -0.06034836612977301 1105 10-11 0.1275474449369156 1105 12-13 -0.09115214206845934 1105 14-15 -0.16982217449921677 1105 16-17 0.08066175785613439 1105 18-19 0.04484646203591325 1105 20-21 0.25066887730471166 1105 22-23 0.30474720276464495 1105 24-25 0.194301878960907 1105 26-27 -0.038323261211623105 1105 28-29 -0.061121129931130724 1105 30-31 -0.067540844533589 1105 32-33 0.08191530680449688 1105 34-35 0.16177512973549568 1105 36-37 0.40275306126567756 1105 38-39 0.31905650555591336 1105 40-41 0.5416214481921386 1105 42-43 0.8750846582271166 1105 44-45 0.514835081955006 1105 46-47 0.5873837283843102 1105 48-49 0.5697057748784502 1105 50-51 0.5707459705427027 1105 52-53 0.6868983251684924 1105 54-55 0.8501290891473587 1105 56-57 0.6466802378186358 1105 58-59 0.6169875084102969 1105 60-61 0.5510368584256824 1105 62-63 0.611481460079041 1105 64-65 0.5858791726435797 1105 66-67 0.6428649686515087 1105 68-69 0.7932969111350943 1105 70-71 1.0110824816562562 1105 72-73 0.8137044415833152 1105 74-75 0.9507716481274464 1105 76-77 1.0172698095756516 1105 78-79 1.0664132688043857 1105 80-81 1.2409678935967179 1105 82-83 0.9725247665093022 1105 84-85 1.0037833033206276 1105 86-87 0.7388059864531904 1105 88-89 0.6644831084821767 1105 90-91 1.048422819649037 1105 92-93 1.3669687251212572 1105 94-95 1.2527233417655914 1105 96-97 1.5170526817919203 1105 98-99 1.7909023691422838 1105 100-101 2.2178779524076866 1106 1 -0.04331316660049822 1106 2 0.009636990647994281 1106 3 -0.13497676022739924 1106 4 -0.01516567719315276 1106 5 0.06746024144304386 1106 6 0.026718143770551706 1106 7 -0.10118598074423346 1106 8 -0.1429133384623711 1106 9 0.02242450363369386 1106 10-11 -0.10059447583823555 1106 12-13 -0.1145989930231579 1106 14-15 -0.11364520100249109 1106 16-17 -0.07084864396775004 1106 18-19 0.07909411093873331 1106 20-21 -0.02855597308001734 1106 22-23 0.008494709176730453 1106 24-25 0.04804226083608398 1106 26-27 0.006924672672667498 1106 28-29 -0.03237362351904949 1106 30-31 0.100790588923239 1106 32-33 0.0818654350461685 1106 34-35 0.15017638365399222 1106 36-37 0.20863792874929032 1106 38-39 0.24959227073117773 1106 40-41 0.3574236710476626 1106 42-43 0.3008897308973957 1106 44-45 0.4703922207409832 1106 46-47 0.4876259626390862 1106 48-49 0.8904453018091942 1106 50-51 0.5495148505654939 1106 52-53 0.6260904026834595 1106 54-55 0.620697627192385 1106 56-57 0.4778358377046388 1106 58-59 0.6098130997468516 1106 60-61 0.8014500815661734 1106 62-63 1.065499698893511 1106 64-65 0.9846537522959053 1106 66-67 1.137478818751255 1106 68-69 1.084882833050166 1106 70-71 1.2853629034288439 1106 72-73 1.4179364165049542 1106 74-75 1.2554667179479075 1106 76-77 1.1409090230267758 1106 78-79 1.0538384611668796 1106 80-81 1.1571120942236774 1106 82-83 0.6101138232206189 1106 84-85 0.7969081252072066 1106 86-87 0.9637062429365173 1106 88-89 1.2640912589523978 1106 90-91 1.571620311812044 1106 92-93 1.3721625125250796 1106 94-95 1.1176991183401164 1106 96-97 1.3962632831028365 1106 98-99 1.714513084445791 1106 100-101 2.0625345497288627 1107 1 0.028116693733686304 1107 2 0.1933047520251634 1107 3 0.2132043361672764 1107 4 0.26283064169240333 1107 5 0.12295665055199834 1107 6 0.0556347412659548 1107 7 0.16425124181883888 1107 8 0.12985002362215425 1107 9 0.1954188376046062 1107 10-11 0.26125095344466587 1107 12-13 0.22322445784119083 1107 14-15 0.5349662609773702 1107 16-17 0.6217737891767143 1107 18-19 0.6313469890649728 1107 20-21 0.5547157076013605 1107 22-23 0.4519652422165308 1107 24-25 0.745626980602232 1107 26-27 0.5147335073473229 1107 28-29 0.6545466911801512 1107 30-31 0.603416089663078 1107 32-33 0.611210593658889 1107 34-35 0.6008016317683271 1107 36-37 0.500276024674811 1107 38-39 0.6380220227972941 1107 40-41 0.8920920098545437 1107 42-43 1.0586940544023946 1107 44-45 1.4103440414490578 1107 46-47 1.1818047493148924 1107 48-49 1.405859968523984 1107 50-51 1.2781319063957355 1107 52-53 1.1997352473187703 1107 54-55 1.3303767928064474 1107 56-57 1.1780911699071694 1107 58-59 1.0912270807638507 1107 60-61 1.5038752863732796 1107 62-63 1.6228276428828323 1107 64-65 1.6420755286140647 1107 66-67 1.647367302637356 1107 68-69 1.7488683612179088 1107 70-71 1.7532975095135122 1107 72-73 1.751947929009912 1107 74-75 1.8324272722804409 1107 76-77 1.8219151943068645 1107 78-79 2.16472301131304 1107 80-81 2.051613880646862 1107 82-83 2.015018366870695 1107 84-85 2.223976045377544 1107 86-87 2.2609336780659 1107 88-89 2.126288559468474 1107 90-91 2.380522654011333 1107 92-93 2.496282834442141 1107 94-95 2.29882709120092 1107 96-97 2.1820278361361467 1107 98-99 2.519508753722917 1107 100-101 2.736602542077918 1108 1 0.05589363495739974 1108 2 -0.06517539654683802 1108 3 -0.13366109574559104 1108 4 0.09542613433909253 1108 5 0.010117218739523537 1108 6 0.08533408373445894 1108 7 0.077411910392847 1108 8 0.1340411594952613 1108 9 -0.03563572245160884 1108 10-11 0.08990376677599698 1108 12-13 0.044767398161418726 1108 14-15 0.3129364461904416 1108 16-17 0.4855468153273961 1108 18-19 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-1.6971659806426338 2316 82-83 -1.3988494728416114 2316 84-85 -1.2831842423183168 2316 86-87 -1.6141225125326137 2316 88-89 -1.4372790619032187 2316 90-91 -1.0991684582414294 2316 92-93 -0.9634445608827988 2316 94-95 -0.9678775710133145 2316 96-97 -1.6411242958957075 2316 98-99 -1.0868359675717159 2316 100-101 -0.7250353335963702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2179.0 3 340.0 4 351.0 5 384.0 6 432.0 7 478.0 8 566.0 9 707.0 10 915.0 11 1375.0 12 2087.0 13 2867.0 14 3168.0 15 4300.0 16 5772.0 17 8516.0 18 7083.0 19 3881.0 20 3440.0 21 3436.0 22 3790.0 23 4021.0 24 4535.0 25 4992.0 26 5733.0 27 6667.0 28 7783.0 29 9074.0 30 11404.0 31 14109.0 32 18059.0 33 23636.0 34 32675.0 35 46622.0 36 67104.0 37 98808.0 38 85149.0 39 12149.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70897770517806 20.489298446010864 14.924569284399544 20.877154564411537 2 14.377316289518685 21.307398122708364 42.41085428800786 21.904431299765093 3 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19.366079582903538 22-23 19.740244643678658 27.294507289364983 33.01743950649635 19.947808560460007 24-25 19.27632032627649 27.64612004846861 33.46599808883941 19.61156153641549 26-27 19.198008963997207 27.560990749288884 33.55153805757728 19.689462229136637 28-29 18.782009525620925 27.439326643258305 34.056213580464686 19.72245025065608 30-31 19.132932597556167 27.566219944816712 33.82144264879779 19.479404808829326 32-33 18.852235268611636 27.770545599676772 33.53922868081458 19.837990450897014 34-35 19.098384482359375 27.76926525725603 33.51142292665382 19.620927333730766 36-37 19.396737293938113 27.503258469804848 33.173931847490806 19.926072388766233 38-39 19.118758281652536 27.995507588333524 33.332610869468844 19.5531232605451 40-41 19.045925569821268 27.793840797794108 33.623321469137494 19.53691216324713 42-43 18.86872576220954 27.921244915975656 32.96792729451083 20.242102027303968 44-45 18.984230137914864 28.310971512570767 32.61186986070579 20.09292848880858 46-47 19.398691213474866 28.365514401183862 31.885562953577683 20.35023143176359 48-49 19.38980024594816 28.732874282651196 31.556942517500158 20.320382953900484 50-51 19.807259833611568 28.732061801417906 31.204828063315116 20.25585030165541 52-53 19.466761289432313 29.22325551596513 31.12093479407228 20.189048400530282 54-55 19.40335862037027 28.950881926058326 31.280219060165678 20.36554039340573 56-57 19.56568418481323 29.593046849452154 30.506613136442972 20.334655829291645 58-59 19.50318145105687 28.992573330969407 31.08435277662865 20.419892441345073 60-61 19.758029959602705 29.139146564062855 31.11172841512776 19.99109506120668 62-63 19.75910925989049 28.949250590601988 31.369191756989203 19.92244839251832 64-65 19.5621724912336 28.763248098971673 31.418482329301444 20.256097080493284 66-67 19.483812497845115 29.183704630418617 30.852645214675878 20.47983765706039 68-69 19.3315995310853 29.167971943927256 31.12746401867778 20.37296450630966 70-71 19.4767456177938 29.145820712480976 30.921063648497565 20.456370021227656 72-73 19.450012855230405 29.07981177184478 30.64066331018735 20.829512062737464 74-75 19.337440549134886 29.326368010213532 30.71063239564822 20.62555904500337 76-77 19.365651458079416 28.979704038993788 31.027626508600093 20.627017994326707 78-79 19.26503263948762 28.786993472102473 30.936519275772877 21.011454612637024 80-81 19.197036424004178 29.41398437423029 30.443452644853643 20.945526556911894 82-83 19.522583162233843 29.233480853277428 30.38500340885356 20.85893257563517 84-85 19.30456934801283 28.864893726012497 30.432701022153218 21.39783590382145 86-87 19.250563008702745 29.135478710094358 30.693874977588013 20.92008330361488 88-89 19.623940886699508 28.657536945812804 30.57990147783251 21.13862068965517 90-91 19.2687684729064 28.62472906403941 31.050738916256158 21.05576354679803 92-93 19.348708450731976 28.852186077670282 30.89226252635312 20.906842945244616 94-95 18.943518982827428 28.800383035695816 30.70984750489878 21.546250476577978 96-97 19.1749947051899 28.828196957084952 30.27015845027065 21.726649887454503 98-99 18.841556281930792 28.933312054348104 30.263938227893995 21.961193435827113 100-101 19.210921100975266 28.421536846107546 30.90713340799776 21.460408644919422 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1084.0 1 665.5 2 320.5 3 389.0 4 341.0 5 330.0 6 513.0 7 1018.0 8 1546.0 9 1761.5 10 1575.0 11 1339.0 12 1357.0 13 1383.5 14 1425.0 15 1494.0 16 1563.5 17 1649.0 18 1753.0 19 1823.0 20 1912.0 21 2165.5 22 2486.0 23 2846.0 24 3212.0 25 3802.0 26 4818.0 27 5837.0 28 6270.5 29 6945.5 30 8232.0 31 9620.0 32 11386.5 33 13535.5 34 15832.0 35 17443.0 36 19572.5 37 21755.5 38 21470.5 39 20423.0 40 19417.5 41 18238.5 42 17402.0 43 17490.0 44 17896.5 45 17733.5 46 16924.5 47 16505.0 48 18646.0 49 18083.0 50 15170.0 51 13295.5 52 11365.0 53 10275.5 54 9550.0 55 8640.5 56 7417.0 57 6333.5 58 5512.5 59 4793.5 60 3946.0 61 3147.5 62 2443.5 63 1756.5 64 1212.5 65 838.0 66 581.0 67 430.0 68 301.0 69 228.0 70 173.5 71 122.0 72 87.0 73 66.0 74 55.5 75 47.0 76 29.5 77 15.0 78 10.5 79 11.5 80 10.0 81 7.5 82 7.0 83 5.5 84 2.5 85 2.0 86 2.0 87 0.0 88 1.5 89 1.5 90 0.5 91 0.5 92 0.0 93 0.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33425811411572015 2 0.3110566685476878 3 0.3000458130238759 4 0.28136668311740914 5 0.27782747955618387 6 0.2284752521190981 7 0.21962724321603497 8 0.2322110781003915 9 0.23810975070243362 10-11 0.23486548077131042 12-13 0.2291634305893364 14-15 0.22660733912845146 16-17 0.22660733912845146 18-19 0.22857356332913217 20-21 0.2124505248835504 22-23 0.20340589356041913 24-25 0.20527380655106578 26-27 0.2191356871658648 28-29 0.23997766369308027 30-31 0.23378405746093603 32-33 0.23820806191246763 34-35 0.22936005300940443 36-37 0.20950118858252934 38-39 0.20969781100259738 40-41 0.2043890056607595 42-43 0.21962724321603497 44-45 0.2166779069150139 46-47 0.21549817239460548 48-49 0.22906511937930235 50-51 0.24027259732318237 52-53 0.2578703039192747 54-55 0.22582084944817918 56-57 0.18934739052555205 58-59 0.1885609008452798 60-61 0.19770384337844507 62-63 0.20763327559188263 64-65 0.1905271250459605 66-67 0.20084980209953418 68-69 0.20330758235038507 70-71 0.19593424159783246 72-73 0.1992768227389896 74-75 0.2019312254099086 76-77 0.2209052889464774 78-79 0.22641071670838336 80-81 0.21510492755446933 82-83 0.21372857061399284 84-85 0.20311095993031703 86-87 0.2060602962313381 88-89 0.21412181545412898 90-91 0.21412181545412898 92-93 0.20822314285208685 94-95 0.20930456616246124 96-97 0.19986668999919388 98-99 0.20566705139120192 100-101 0.19357477255701558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 508589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.52307427612128 #Duplication Level Percentage of deduplicated Percentage of total 1 85.97232164824241 52.03309208807164 2 8.332471134519704 10.08613538756345 3 2.1347779154739634 3.8760996702376214 4 0.9002011623635571 2.1793176725272105 5 0.5023840568314013 1.5202913793373016 6 0.3399859819647276 1.2346198103574741 7 0.2312573149281689 0.9797482553805754 8 0.18849680251454273 0.9126724783519227 9 0.14503144244922397 0.7899973887354651 >10 1.085082084547601 13.05549156345387 >50 0.11470141427999908 4.780597061407424 >100 0.05003971006247923 5.252973272774451 >500 0.0022745322755672375 1.0645181507816293 >1k 9.747995466716733E-4 2.234445821019928 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4843 0.9522423803896664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3944 0.7754788247484707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 0.5063027316752821 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 946 0.18600480938439487 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 929 0.18266222824323766 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 819 0.1610337620357499 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 769 0.15120264103234635 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 703 0.13822556130785368 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 654 0.12859106272451823 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 593 0.11659709510036592 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.831121003403534E-5 0.0 18-19 0.0 0.0 0.0 1.966224200680707E-4 0.0 20-21 0.0 0.0 0.0 1.966224200680707E-4 0.0 22-23 0.0 0.0 0.0 3.932448401361414E-4 0.0 24-25 0.0 0.0 0.0 9.831121003403534E-4 0.0 26-27 0.0 0.0 0.0 9.831121003403534E-4 0.0 28-29 0.0 0.0 0.0 0.001966224200680707 0.0 30-31 1.966224200680707E-4 0.0 0.0 0.004817249291667732 0.0 32-33 1.966224200680707E-4 0.0 0.0 0.00992943221343757 0.0 34-35 1.966224200680707E-4 0.0 0.0 0.013665258194730912 0.0 36-37 1.966224200680707E-4 0.0 0.0 0.01917068595663689 0.0 38-39 1.966224200680707E-4 0.0 0.0 0.027822072439632 0.0 40-41 1.966224200680707E-4 0.0 0.0 0.03883292796344396 0.0 42-43 1.966224200680707E-4 0.0 0.0 0.053088053418379084 0.0 44-45 1.966224200680707E-4 0.0 0.0 0.06291917442178262 0.0 46-47 1.966224200680707E-4 0.0 0.0 0.08602230877978093 0.0 48-49 1.966224200680707E-4 0.0 0.0 0.11227140185886836 0.0 50-51 1.966224200680707E-4 0.0 0.0 0.12800119546431402 0.0 52-53 1.966224200680707E-4 0.0 0.0 0.15759286968455866 0.0 54-55 1.966224200680707E-4 0.0 0.0 0.19829371063864928 0.0 56-57 1.966224200680707E-4 0.0 0.0 0.2583618599694449 0.0 58-59 1.966224200680707E-4 0.0 0.0 0.32944086482405244 0.0 60-61 1.966224200680707E-4 0.0 0.0 0.4250976721871688 0.0 62-63 1.966224200680707E-4 0.0 0.0 0.5134794500077666 0.0 64-65 1.966224200680707E-4 0.0 0.0 0.6000916260477518 0.0 66-67 1.966224200680707E-4 0.0 0.0 0.723177261010364 0.0 68-69 1.966224200680707E-4 0.0 0.0 0.8753630141430506 0.0 70-71 1.966224200680707E-4 0.0 0.0 0.9745590250673923 0.0 72-73 1.966224200680707E-4 0.0 0.0 1.0951868797791537 0.0 74-75 1.966224200680707E-4 0.0 0.0 1.246192898391432 0.0 76-77 1.966224200680707E-4 0.0 0.0 1.4014262990351738 0.0 78-79 1.966224200680707E-4 0.0 0.0 1.5823189254977987 0.0 80-81 1.966224200680707E-4 0.0 0.0 1.8439250553983668 0.0 82-83 1.966224200680707E-4 0.0 0.0 2.1239153815752996 0.0 84-85 1.966224200680707E-4 0.0 0.0 2.4607295871519046 0.0 86-87 1.966224200680707E-4 0.0 0.0 2.8916276207310814 0.0 88-89 1.966224200680707E-4 0.0 0.0 3.4040256474284734 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATCG 25 0.005019807 56.999588 9 GGCCGAT 25 0.005019807 56.999588 7 TACCTGG 415 0.0 48.100384 2 ACCTGGG 570 0.0 35.01355 3 GTACCTG 595 0.0 33.558937 1 GGGCCGA 75 3.0761742E-4 31.666435 6 GTATAGG 145 2.374145E-8 29.50872 1 GGTATCA 4025 0.0 29.410967 1 GTGTAGG 215 1.8189894E-12 28.746222 1 GTAGGGT 120 5.3577496E-6 27.719059 3 CTGGTCG 155 4.577123E-8 27.580446 9 GTACACG 70 0.0083512915 27.166758 1 GTAGGTA 70 0.008363356 27.15872 2 GTATCAA 5600 0.0 27.08186 1 CATGGGG 1215 0.0 26.991076 4 GTACATG 4225 0.0 25.543182 1 GTATAGT 150 1.050068E-6 25.355639 1 TAGGGTC 150 1.0527801E-6 25.348137 4 TACATGG 4280 0.0 24.874342 2 CATGGGC 490 0.0 24.248857 4 >>END_MODULE