FastQCFastQC Report
Thu 26 May 2016
SRR937721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508589
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53741.0566488854458118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48880.9610903892927295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31970.6286018769576219No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.22611578307828128No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10750.21136910157317595No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10190.20035824604936406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9770.19210010440650505No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6990.1374390716275814No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6600.12977079724492666No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.12288901254254418No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5700.11207477943880029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC250.00501428457.015463
TACCTGG5050.031.9888742
GTACCTG5300.031.3918691
GTATAGG2750.029.3860551
GCCGTAC650.00581930329.2530841
ACCTGGG5450.027.8974763
GTCTTAC1151.273762E-424.8015271
GGTATCA50850.024.3991431
CATGGGC5300.024.2046764
GTACATG45550.023.898581
ATGGGCA4000.023.7564415
TACATGG46600.023.0427282
GGTAGTT1452.3314622E-522.9304897
GAGTACT39600.022.7898612-13
CCTTATA2303.603418E-922.7235532
GTCTAAT1050.00218483622.6363161
ACATGGG46150.022.5467433
GTATCAA66400.022.4079071
TACACTG3900.021.9290265
CATGGGT4550.021.9290244
Produced by FastQC (version 0.11.5)