##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937721_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 508589 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28754849200435 33.0 31.0 34.0 31.0 34.0 2 32.484813867386045 34.0 31.0 34.0 31.0 34.0 3 32.53018842326515 34.0 31.0 34.0 31.0 34.0 4 36.1285419071195 37.0 35.0 37.0 35.0 37.0 5 36.07044981311039 37.0 35.0 37.0 35.0 37.0 6 36.1383513996567 37.0 35.0 37.0 35.0 37.0 7 36.09524390028098 37.0 35.0 37.0 35.0 37.0 8 36.07123826901486 37.0 35.0 37.0 35.0 37.0 9 37.754577861495235 39.0 37.0 39.0 35.0 39.0 10-11 37.68363255988628 39.0 37.0 39.0 35.0 39.0 12-13 37.57629343143481 39.0 37.0 39.0 35.0 39.0 14-15 38.73033431710084 40.0 38.0 41.0 34.5 41.0 16-17 38.69433373509848 40.0 38.0 41.0 34.5 41.0 18-19 38.75781918209006 40.0 38.0 41.0 35.0 41.0 20-21 38.699570773256994 40.0 38.0 41.0 34.0 41.0 22-23 38.64809600679527 40.0 38.0 41.0 34.0 41.0 24-25 38.64940157966452 40.0 38.0 41.0 34.0 41.0 26-27 38.44538222415349 40.0 38.0 41.0 34.0 41.0 28-29 38.39987101569243 40.0 38.0 41.0 34.0 41.0 30-31 38.33480767377981 40.0 37.5 41.0 34.0 41.0 32-33 38.115403597010555 40.0 37.0 41.0 34.0 41.0 34-35 37.931287345970915 40.0 37.0 41.0 33.5 41.0 36-37 37.72708906405762 40.0 37.0 41.0 33.0 41.0 38-39 37.565907835206815 40.0 36.0 41.0 33.0 41.0 40-41 37.354205458631625 40.0 36.0 41.0 33.0 41.0 42-43 37.0652638967811 40.0 36.0 41.0 32.5 41.0 44-45 36.62301976645189 40.0 36.0 41.0 31.0 41.0 46-47 36.06173943990137 39.5 35.5 41.0 29.5 41.0 48-49 35.544415038469175 39.0 35.0 41.0 27.0 41.0 50-51 35.23229169329262 39.0 35.0 41.0 25.5 41.0 52-53 34.964774110332705 39.0 35.0 40.5 24.0 41.0 54-55 34.7490075483347 39.0 35.0 40.0 23.5 41.0 56-57 34.50586426367853 38.5 35.0 40.0 23.0 41.0 58-59 34.257555708047164 38.0 34.0 40.0 22.0 41.0 60-61 33.917095139690396 38.0 34.0 40.0 20.5 41.0 62-63 33.50962663368654 38.0 33.5 40.0 18.5 41.0 64-65 33.14174215329077 37.0 33.0 40.0 15.0 41.0 66-67 32.72411121750569 36.5 32.5 39.0 10.5 41.0 68-69 32.25591292772749 36.0 32.0 39.0 8.0 40.0 70-71 31.842962588652135 35.5 31.5 38.5 7.0 40.0 72-73 31.365755059586427 35.0 31.0 38.0 2.0 40.0 74-75 30.872864926296085 35.0 31.0 37.0 2.0 39.0 76-77 29.605821203368535 33.5 29.5 36.0 2.0 38.5 78-79 30.179329478223085 34.0 30.0 36.0 2.0 39.0 80-81 29.997565814439557 34.0 30.0 36.0 2.0 38.0 82-83 29.60199886352241 34.0 30.0 35.5 2.0 37.0 84-85 29.177149918696628 34.0 29.5 35.0 2.0 37.0 86-87 28.833817679894768 34.0 29.0 35.0 2.0 36.0 88-89 28.418870640143613 34.0 29.0 35.0 2.0 36.0 90-91 27.92168725631109 34.0 28.5 35.0 2.0 35.5 92-93 27.477406117709975 33.5 27.0 35.0 2.0 35.0 94-95 26.991697618312624 33.0 26.0 35.0 2.0 35.0 96-97 26.503149891169492 33.0 25.0 35.0 2.0 35.0 98-99 25.724470053422312 32.5 24.0 34.5 2.0 35.0 100-101 23.3136963245371 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12760782965864337 1101 2 0.0671962649759621 1101 3 0.03291494136809803 1101 4 0.07822755330187903 1101 5 -0.00954013457450742 1101 6 -0.026250238328856312 1101 7 -0.0025936910996051665 1101 8 -0.06083272162459963 1101 9 0.15964848359228512 1101 10-11 0.03906345363143515 1101 12-13 2.2270679968983131E-4 1101 14-15 0.04350127231950296 1101 16-17 0.26478957628101796 1101 18-19 0.11606030302235837 1101 20-21 0.19612280098827028 1101 22-23 0.17394158666397885 1101 24-25 0.048273724651060945 1101 26-27 -0.06541864131066433 1101 28-29 0.14051267825728786 1101 30-31 0.05219930676714313 1101 32-33 -0.04307913283278708 1101 34-35 -0.08165040404148982 1101 36-37 -0.1288359069630971 1101 38-39 0.11542652454195945 1101 40-41 0.06525640682043132 1101 42-43 0.14768864305498397 1101 44-45 0.14118869025276837 1101 46-47 0.34577297079597 1101 48-49 0.2366856080536266 1101 50-51 0.18940773715672066 1101 52-53 0.23039826111175898 1101 54-55 0.2933549628377605 1101 56-57 0.3044661875063568 1101 58-59 0.35341414729805365 1101 60-61 0.3461535612526987 1101 62-63 0.5139860903849325 1101 64-65 0.4471323580185924 1101 66-67 0.5241888680027458 1101 68-69 0.41933140295692084 1101 70-71 0.3633480256253989 1101 72-73 0.22838777502196095 1101 74-75 0.37510179562053736 1101 76-77 0.2844003115255056 1101 78-79 0.5084179256601402 1101 80-81 0.5250710435134103 1101 82-83 0.5178033334116385 1101 84-85 0.3864829123753637 1101 86-87 0.5167375938663703 1101 88-89 0.6788378470426082 1101 90-91 0.7046978085682589 1101 92-93 0.8023555648909593 1101 94-95 0.8443718870663872 1101 96-97 0.7301193285288363 1101 98-99 0.7190823161332887 1101 100-101 1.1580879073221517 1102 1 0.28493498105890325 1102 2 0.2956689392330034 1102 3 0.2654873335554129 1102 4 0.18234592361641688 1102 5 0.22710532927983706 1102 6 0.15260328716210125 1102 7 0.24115109860427708 1102 8 0.24302603795377564 1102 9 0.22116328559152265 1102 10-11 0.23970410593196334 1102 12-13 0.23821761355680593 1102 14-15 0.3778243240018355 1102 16-17 0.4379998038781707 1102 18-19 0.4491728530316905 1102 20-21 0.4760037302576663 1102 22-23 0.427697758128879 1102 24-25 0.3278158657163672 1102 26-27 0.3388337216418833 1102 28-29 0.49931032272761655 1102 30-31 0.5524581561814941 1102 32-33 0.39669650563071457 1102 34-35 0.4456391761275995 1102 36-37 0.5521017369743006 1102 38-39 0.519850184901081 1102 40-41 0.5981455088794263 1102 42-43 0.6246624650046542 1102 44-45 0.4603424049321916 1102 46-47 0.4922417699250232 1102 48-49 0.6622968542993561 1102 50-51 0.6992516281202086 1102 52-53 0.8759230212002294 1102 54-55 0.7417658938962646 1102 56-57 0.7086804843685712 1102 58-59 0.7359878717985282 1102 60-61 0.9276073813752035 1102 62-63 0.8564248325290436 1102 64-65 1.0057655569786235 1102 66-67 0.9195775418182208 1102 68-69 1.0168548231189973 1102 70-71 0.7598977151964306 1102 72-73 1.1669167490266297 1102 74-75 1.1622057655330984 1102 76-77 1.1023149749641696 1102 78-79 1.1596202431237188 1102 80-81 1.4280272560896847 1102 82-83 1.598486315204628 1102 84-85 1.449889599064793 1102 86-87 1.5243013260181328 1102 88-89 1.4652908868102514 1102 90-91 1.5263486587743884 1102 92-93 1.5833461206939425 1102 94-95 1.841168625563764 1102 96-97 1.7805968676322266 1102 98-99 1.865827094417348 1102 100-101 2.065956548857585 1103 1 0.12568263713939132 1103 2 0.09456150149961218 1103 3 0.20301945181914505 1103 4 0.038486079154459674 1103 5 0.08603192263121429 1103 6 0.11580146684165982 1103 7 0.10260432870237679 1103 8 -0.03896803515595337 1103 9 0.12595761450538134 1103 10-11 0.09888193547962487 1103 12-13 0.1534817827072814 1103 14-15 0.15811898409068448 1103 16-17 0.1781559129146828 1103 18-19 0.09639583657571649 1103 20-21 0.16091928063623584 1103 22-23 0.18232992549786076 1103 24-25 0.04469836711530206 1103 26-27 0.12263141369482611 1103 28-29 0.24742961995145407 1103 30-31 0.172386325469013 1103 32-33 0.37633780886138624 1103 34-35 0.3155580668056004 1103 36-37 0.24692041866946823 1103 38-39 0.27006073796330554 1103 40-41 0.4130286840481574 1103 42-43 0.5345835825489402 1103 44-45 0.647033024686209 1103 46-47 0.6214192854274359 1103 48-49 0.6987318126740831 1103 50-51 0.775216318014806 1103 52-53 0.5405605273383856 1103 54-55 0.35255463280475396 1103 56-57 0.38456794768237756 1103 58-59 0.42155221110443364 1103 60-61 0.46695939183576485 1103 62-63 0.554483890164903 1103 64-65 0.555286923475137 1103 66-67 0.6118538845043915 1103 68-69 0.6968341532319471 1103 70-71 0.6448386746903054 1103 72-73 0.46573650989544646 1103 74-75 0.6346590013411095 1103 76-77 0.4404096624605991 1103 78-79 0.6380246363312025 1103 80-81 0.7613884252515852 1103 82-83 0.8516867217504753 1103 84-85 0.7172034844325701 1103 86-87 0.6558327533823203 1103 88-89 0.8360848217400765 1103 90-91 0.6050690956969724 1103 92-93 0.8114314724817184 1103 94-95 0.7490748573634143 1103 96-97 0.7211809346894498 1103 98-99 1.0171433722388983 1103 100-101 1.5614157401054278 1104 1 0.16852620721068234 1104 2 0.17049230886606637 1104 3 0.042201584318107166 1104 4 0.09775807778289902 1104 5 0.06718182333556655 1104 6 0.06741627117923343 1104 7 0.14619261610254597 1104 8 -0.023100376961565416 1104 9 0.041322365266168504 1104 10-11 0.12490060877551912 1104 12-13 0.04512005367723049 1104 14-15 0.22653386129268682 1104 16-17 0.07271322578882433 1104 18-19 0.2100839911054564 1104 20-21 0.08380442629366058 1104 22-23 0.10957086387741555 1104 24-25 0.2531942167002583 1104 26-27 0.26534300628265584 1104 28-29 0.12387530023377025 1104 30-31 0.2351676035968282 1104 32-33 0.3076273663885658 1104 34-35 0.23741007343483034 1104 36-37 0.04410281548057782 1104 38-39 0.25073291232359907 1104 40-41 0.2968652819936608 1104 42-43 0.3437939678731823 1104 44-45 0.2888748874439173 1104 46-47 0.5534794557301765 1104 48-49 0.5724263249824588 1104 50-51 0.8036620197278381 1104 52-53 0.9669719184774976 1104 54-55 0.9308115656408944 1104 56-57 0.8719443638954303 1104 58-59 0.7752634750879821 1104 60-61 0.847253671263708 1104 62-63 0.6564717675240672 1104 64-65 0.8572662285485038 1104 66-67 0.8997549960441162 1104 68-69 0.907973124271912 1104 70-71 0.9047021610389407 1104 72-73 0.7689704047134995 1104 74-75 1.1659880271008305 1104 76-77 0.921949780758144 1104 78-79 0.9816866811071137 1104 80-81 0.9363407419118239 1104 82-83 0.8309917951149544 1104 84-85 0.7305458901017055 1104 86-87 0.8678155588057237 1104 88-89 0.8662780196312951 1104 90-91 0.8340250270043903 1104 92-93 0.797362208412455 1104 94-95 0.7853159814758293 1104 96-97 0.8991576609335041 1104 98-99 1.0628666945711345 1104 100-101 1.167445985987161 1105 1 -0.11465536217778549 1105 2 0.024102300407093935 1105 3 -0.10958723988451169 1105 4 -0.0807611041845604 1105 5 -0.06641771888466508 1105 6 -0.11788750550385174 1105 7 -0.05593954292616843 1105 8 -0.01659691873396696 1105 9 0.16386304297925847 1105 10-11 0.07395021816995495 1105 12-13 -0.09502026577343514 1105 14-15 -0.030541821645066136 1105 16-17 0.13537680741792002 1105 18-19 -0.07186978308970282 1105 20-21 -0.01512203039141724 1105 22-23 0.032364066843022954 1105 24-25 -0.005156532167724492 1105 26-27 -0.12967739477222295 1105 28-29 -0.08961800722505586 1105 30-31 -0.07494211427151498 1105 32-33 -0.14971203750256734 1105 34-35 0.036747039151102 1105 36-37 -0.00741979983514085 1105 38-39 -0.04596435592539194 1105 40-41 -0.05135335760428461 1105 42-43 0.05450078978689987 1105 44-45 0.10015269010102656 1105 46-47 0.07104799829872377 1105 48-49 0.40528695094653244 1105 50-51 0.2394447236139854 1105 52-53 0.34634321767638454 1105 54-55 0.17504951160297821 1105 56-57 0.15872721705758863 1105 58-59 0.41145917593885173 1105 60-61 0.5826942842905254 1105 62-63 0.40192755694538107 1105 64-65 0.7699036868756153 1105 66-67 0.6830087155047337 1105 68-69 0.7065388305962372 1105 70-71 0.5035788590535688 1105 72-73 0.7871858099978688 1105 74-75 0.4566246892892174 1105 76-77 0.7287119840720706 1105 78-79 0.8519073608105501 1105 80-81 0.8256637752003755 1105 82-83 0.6805190877456937 1105 84-85 0.7447470146476611 1105 86-87 0.9826038356629638 1105 88-89 1.040382363563225 1105 90-91 0.8360054220882347 1105 92-93 0.7019487138610288 1105 94-95 0.9935012827645799 1105 96-97 1.393167960978289 1105 98-99 1.3411233995519751 1105 100-101 1.6539899975311734 1106 1 -0.5852452738335252 1106 2 -0.4834497087123175 1106 3 -0.5577918565844229 1106 4 -0.3934855270970701 1106 5 -0.3443031563312289 1106 6 -0.4123161176440604 1106 7 -0.5301487193297021 1106 8 -0.4298628064512684 1106 9 -0.7767610664536235 1106 10-11 -0.7717608106131735 1106 12-13 -0.8215662902818366 1106 14-15 -0.6706030926624535 1106 16-17 -0.5723248169764901 1106 18-19 -1.0681365257514202 1106 20-21 -1.069268510870188 1106 22-23 -0.8010286376311839 1106 24-25 -1.0713007327946897 1106 26-27 -0.979114556356727 1106 28-29 -1.1883213415083276 1106 30-31 -0.9662786773949108 1106 32-33 -0.9946322426892209 1106 34-35 -1.417171456146697 1106 36-37 -1.1048050947909687 1106 38-39 -1.3477027429302382 1106 40-41 -1.4982613085874732 1106 42-43 -1.6364795464912376 1106 44-45 -1.982278201890999 1106 46-47 -1.599199935585574 1106 48-49 -1.7920627213247684 1106 50-51 -1.8785661056821112 1106 52-53 -1.738032672890732 1106 54-55 -2.149373400907713 1106 56-57 -2.270191278240212 1106 58-59 -2.0095653324680995 1106 60-61 -2.0655103324093886 1106 62-63 -2.0121790261267236 1106 64-65 -2.098377874822873 1106 66-67 -2.00012038110399 1106 68-69 -2.0395213004952453 1106 70-71 -2.247846048327453 1106 72-73 -1.905468792452968 1106 74-75 -1.9718022129621353 1106 76-77 -2.077607480162751 1106 78-79 -1.6983277489073174 1106 80-81 -2.184723799607262 1106 82-83 -2.3936900886578414 1106 84-85 -2.336914427358611 1106 86-87 -2.2067449816639098 1106 88-89 -2.8921340228716588 1106 90-91 -2.970021936133481 1106 92-93 -2.9592296845430752 1106 94-95 -2.7623194638868895 1106 96-97 -3.160907274131432 1106 98-99 -3.101274719570281 1106 100-101 -2.6887785975101473 1107 1 0.1650163740065267 1107 2 0.17016464041612522 1107 3 0.1379658394710077 1107 4 0.05816375652918282 1107 5 0.13307783901324655 1107 6 0.13894881432749884 1107 7 0.044835942693467246 1107 8 0.17944283431527452 1107 9 0.20744081741082 1107 10-11 0.21438087620338564 1107 12-13 0.23933450007508839 1107 14-15 0.010957952485334488 1107 16-17 0.09072238790279386 1107 18-19 0.309326572880785 1107 20-21 0.45346327302674183 1107 22-23 0.26382393734446197 1107 24-25 0.17993983886073295 1107 26-27 0.33887779269954166 1107 28-29 0.39204514591452977 1107 30-31 0.26504056905931606 1107 32-33 0.40546790527714194 1107 34-35 0.5350417236865681 1107 36-37 0.345441215072249 1107 38-39 0.6381457178586771 1107 40-41 0.5043912591702906 1107 42-43 0.5736281802401422 1107 44-45 0.880870955422516 1107 46-47 0.31438860965243265 1107 48-49 0.2396868907146441 1107 50-51 0.12198876818558801 1107 52-53 -0.031186521820679047 1107 54-55 0.0774211423814748 1107 56-57 0.32012233827427394 1107 58-59 0.08008965327660178 1107 60-61 0.2994613374451731 1107 62-63 0.3789980282599359 1107 64-65 0.26266456626125034 1107 66-67 0.2738609967876684 1107 68-69 0.1995255795798201 1107 70-71 0.38448911353331283 1107 72-73 0.3538963957530292 1107 74-75 0.25548404781955725 1107 76-77 0.01545343867228155 1107 78-79 0.5880954348957772 1107 80-81 0.5217298797027041 1107 82-83 0.3810762327539763 1107 84-85 0.6474047469168944 1107 86-87 0.5885065611561124 1107 88-89 0.3429836284329717 1107 90-91 0.5771739208083062 1107 92-93 0.9151432628747322 1107 94-95 0.732704715982468 1107 96-97 0.8152241272183112 1107 98-99 1.2632548556579835 1107 100-101 1.718291307574841 1108 1 -0.03534501735019546 1108 2 -0.02877126281129705 1108 3 -0.04406999850520066 1108 4 -0.019841563954678065 1108 5 -0.2198659947467334 1108 6 -0.12765762044638507 1108 7 -0.03122689924800426 1108 8 -0.09360841298683908 1108 9 0.14202396251948812 1108 10-11 -0.015130241600161298 1108 12-13 -0.1846754381872202 1108 14-15 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-0.46189275103883176 2316 60-61 -0.47893096925917433 2316 62-63 -0.03356940045827628 2316 64-65 -0.2440542238748904 2316 66-67 -0.590406598896891 2316 68-69 -0.6187781065234432 2316 70-71 -0.5456097819200636 2316 72-73 -0.12049674578306124 2316 74-75 -0.25226222754648475 2316 76-77 -0.1316455209989229 2316 78-79 -0.3742970002442192 2316 80-81 -0.16792141749739642 2316 82-83 -0.4398282753374687 2316 84-85 -0.39623929366877064 2316 86-87 -0.36331401423561616 2316 88-89 -0.4757053646315015 2316 90-91 -0.5126507645243414 2316 92-93 -0.3209214686947526 2316 94-95 -0.0779490214950549 2316 96-97 0.18785609485252763 2316 98-99 -0.9263081420301731 2316 100-101 -0.909245296586473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 3.0 5 6.0 6 22.0 7 58.0 8 123.0 9 286.0 10 493.0 11 961.0 12 1506.0 13 2199.0 14 2796.0 15 4164.0 16 6124.0 17 9380.0 18 8319.0 19 4377.0 20 3097.0 21 2925.0 22 3025.0 23 3121.0 24 3500.0 25 3988.0 26 4649.0 27 5624.0 28 7034.0 29 8484.0 30 10436.0 31 12878.0 32 16650.0 33 22254.0 34 30737.0 35 44896.0 36 69395.0 37 110846.0 38 95216.0 39 9010.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.876978998861134 20.965537046543968 15.602644369306907 19.554839585287993 2 15.153689914646208 22.423607274243054 41.4480061503493 20.974696660761442 3 19.397981474235582 28.475252119098133 31.06535925865483 21.06140714801146 4 11.989248686070678 18.395207131888423 39.003792846483115 30.61175133555779 5 11.54916338766545 40.4641531917906 34.33659955283665 13.650083867707293 6 28.35491919801647 38.771778390802794 18.24164502181526 14.631657389365479 7 26.246930232466685 35.96420685465081 21.396058506967318 16.39280440591519 8 26.367263153548347 35.50253741233098 20.005544752245918 18.124654681874755 9 27.77507968123573 17.011771784289476 21.781438450300733 33.43171008417406 10-11 24.112495551417744 28.811378146204497 27.002156947948148 20.073969354429607 12-13 25.774447651782694 26.755362241326424 28.315170797316323 19.155019309574563 14-15 22.993222425180253 27.119048976678616 28.30713994993993 21.5805886482012 16-17 19.63766420429856 30.736213327460877 29.07986606080745 20.546256407433113 18-19 19.444187742951577 28.28344694832173 33.59824927397172 18.674116034754977 20-21 20.616609727922924 27.114999877110623 33.854449824268194 18.413940570698255 22-23 20.30699141336385 27.18042946944538 33.60939555351065 18.90318356368012 24-25 19.966908446702544 27.467955461089407 33.99237891499816 18.57275717720989 26-27 19.84051767361473 27.453139408906708 34.144475926249996 18.56186699122857 28-29 19.35302468431836 27.41435992622625 34.516951052141046 18.715664337314337 30-31 19.681609315183774 27.645800440040976 34.23324137958155 18.439348865193704 32-33 19.38018714521942 27.904850478480657 33.95757674664611 18.757385629653808 34-35 19.69979688904007 27.573148455825823 34.119495309572166 18.607559345561935 36-37 19.92754442450906 27.548455280556443 33.77845503477769 18.745545260156806 38-39 19.83035417596527 27.822957240522307 33.95570883365547 18.390979749856957 40-41 19.641419339996872 27.813939953420103 34.01610535826115 18.528535348321874 42-43 19.500600844525824 28.074878405195385 33.47343205146612 18.95108869881267 44-45 19.808332464917644 28.442121241316663 32.92088503683721 18.828661256928484 46-47 20.081441006392193 28.542595297971445 32.20193515785831 19.17402853777805 48-49 20.11742290926465 28.952553043813374 31.6631897268718 19.266834320050176 50-51 20.48569670205215 29.15074844324199 31.160229576337674 19.20332527836819 52-53 20.456518383722795 29.360770052803005 31.104026142942676 19.07868542053153 54-55 20.05656827025358 29.338719476827062 31.115989531822358 19.488722721096995 56-57 20.521481982504536 29.797439582845875 30.42643470464363 19.254643730005956 58-59 20.419828191329344 29.205311164810876 30.909241057120777 19.465619586738995 60-61 20.520793804034298 29.411076527412117 30.89882006885717 19.169309599696415 62-63 20.56149464498839 28.993745440817637 31.43363305144232 19.011126862751652 64-65 20.27934147219071 29.133150736645895 31.105273609928645 19.48223418123475 66-67 20.484418656321708 29.442732737043077 30.568297780722748 19.504550825912474 68-69 20.234020004365018 29.496607280141724 30.873652399088456 19.395720316404798 70-71 20.268133994246828 29.548908843879833 30.71841899844472 19.464538163428625 72-73 20.448731687079352 29.294381121101715 30.447178369960813 19.80970882185812 74-75 20.380307084895662 29.795276736225123 30.197566207684396 19.626849971194815 76-77 20.303526029859082 29.437227309281166 30.67506375481971 19.58418290604004 78-79 20.235101427675392 29.12233650354215 30.638491984687043 20.00407008409541 80-81 20.26479141310567 29.812677820401152 30.10967598591397 19.81285478057921 82-83 20.4403171132626 29.84360621957262 29.98979527633369 19.726281390831087 84-85 20.124560670484428 29.313146705335857 30.124708137734412 20.4375844864453 86-87 20.06600614641685 29.390529484515 30.31170552253391 20.231758846534234 88-89 20.433690071944145 28.99295895113736 30.450619262312006 20.12273171460649 90-91 20.097538912323138 29.02116948369271 30.897810290748552 19.9834813132356 92-93 20.00943822875907 29.4164028550642 30.574060601293823 20.00009831488291 94-95 19.764092420402328 29.347665796940163 30.399497433094307 20.488744349563206 96-97 20.107788410681316 29.114176673109327 30.076348485712433 20.701686430496924 98-99 19.808134899373066 29.28479226247649 30.107661135323095 20.799411702827346 100-101 19.911483540579713 29.370807464409538 30.148497619436256 20.569211375574493 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 614.0 1 417.5 2 362.5 3 459.0 4 336.5 5 292.5 6 516.5 7 1048.5 8 1609.0 9 1843.0 10 1661.0 11 1396.5 12 1372.0 13 1457.5 14 1534.5 15 1589.5 16 1635.0 17 1743.5 18 1860.5 19 1983.5 20 2146.5 21 2314.5 22 2635.5 23 3074.5 24 3393.0 25 3970.0 26 5055.0 27 6043.5 28 6489.5 29 7056.0 30 8211.0 31 9409.0 32 11108.0 33 13371.0 34 15660.0 35 17357.0 36 19552.0 37 21365.0 38 21175.5 39 20632.0 40 19754.5 41 18587.0 42 17439.0 43 17323.5 44 17885.0 45 17952.5 46 17164.5 47 16391.0 48 18795.0 49 18173.5 50 14987.5 51 13276.5 52 11057.5 53 10040.5 54 9398.5 55 8485.5 56 7298.0 57 6179.0 58 5351.0 59 4690.0 60 3883.5 61 3075.5 62 2378.0 63 1683.5 64 1153.5 65 810.5 66 549.5 67 387.0 68 272.0 69 200.0 70 147.0 71 102.0 72 76.0 73 58.0 74 50.0 75 35.0 76 19.5 77 11.5 78 9.5 79 4.0 80 0.5 81 1.5 82 3.0 83 2.5 84 0.5 85 0.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036965014972797285 2 0.0 3 0.0 4 0.0 5 0.009831121003403534 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006488539862246332 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.9493363010210606E-4 22-23 0.0015729793605445653 24-25 0.0 26-27 0.0011797345204084242 28-29 0.0013763569404764949 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.9493363010210606E-4 38-39 0.0 40-41 9.831121003403533E-5 42-43 0.027133893969393755 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.831121003403533E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9662242006807066E-4 84-85 2.9493363010210606E-4 86-87 0.0 88-89 0.0 90-91 0.01455005908503723 92-93 0.0037358259812933434 94-95 0.0 96-97 0.0 98-99 4.915560501701767E-4 100-101 0.018875752326534788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 508589.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.56060795589142 #Duplication Level Percentage of deduplicated Percentage of total 1 85.13622793678636 49.85629267049556 2 8.79768870754791 10.303959986413727 3 2.2457221930926528 3.9453257078263047 4 0.9867536658437993 2.311395782980697 5 0.5707532674880245 1.6711829168455112 6 0.3574288612448473 1.2558750849288127 7 0.2562326306990028 1.0503597042309707 8 0.19090344447458005 0.8943537415444134 9 0.16131921953479986 0.8502256413835014 >10 1.117015784842727 13.077956789942547 >50 0.11503619950433662 4.6169277109625035 >100 0.06121809423444126 6.180525634511079 >500 0.001681815775671463 0.6955233456180838 >1k 0.001681815775671463 2.231544599973739 >5k 3.363631551342926E-4 1.058550682342561 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5374 1.0566488854458118 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4888 0.9610903892927295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3197 0.6286018769576219 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1150 0.22611578307828128 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1075 0.21136910157317595 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1019 0.20035824604936406 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 977 0.19210010440650505 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 699 0.1374390716275814 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 660 0.12977079724492666 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 625 0.12288901254254418 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 570 0.11207477943880029 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.831121003403534E-5 0.0 18-19 1.966224200680707E-4 0.0 0.0 1.966224200680707E-4 0.0 20-21 1.966224200680707E-4 0.0 0.0 1.966224200680707E-4 0.0 22-23 1.966224200680707E-4 0.0 0.0 3.932448401361414E-4 0.0 24-25 1.966224200680707E-4 0.0 0.0 9.831121003403534E-4 0.0 26-27 1.966224200680707E-4 0.0 0.0 9.831121003403534E-4 0.0 28-29 1.966224200680707E-4 0.0 0.0 0.0017696017806126361 0.0 30-31 1.966224200680707E-4 0.0 0.0 0.0046206268715996614 0.0 32-33 1.966224200680707E-4 0.0 0.0 0.009732809793369499 0.0 34-35 1.966224200680707E-4 0.0 0.0 0.013665258194730912 0.0 36-37 1.966224200680707E-4 0.0 0.0 0.019563930796773033 0.0 38-39 1.966224200680707E-4 0.0 0.0 0.028215317279768143 0.0 40-41 1.966224200680707E-4 0.0 0.0 0.0395211064336822 0.0 42-43 1.966224200680707E-4 0.0 0.0 0.05387454309865136 0.0 44-45 1.966224200680707E-4 0.0 0.0 0.06350904168198683 0.0 46-47 1.966224200680707E-4 0.0 0.0 0.08690710967008725 0.0 48-49 1.966224200680707E-4 0.0 0.0 0.11345113637927678 0.0 50-51 1.966224200680707E-4 0.0 0.0 0.12947586361482455 0.0 52-53 1.966224200680707E-4 0.0 0.0 0.15926416025513723 0.0 54-55 1.966224200680707E-4 0.0 0.0 0.1999650012092279 0.0 56-57 1.966224200680707E-4 0.0 0.0 0.2603280841701256 0.0 58-59 1.966224200680707E-4 0.0 0.0 0.3308172217645289 0.0 60-61 1.966224200680707E-4 0.0 0.0 0.4264740291276453 0.0 62-63 1.966224200680707E-4 0.0 0.0 0.5144625621081069 0.0 64-65 1.966224200680707E-4 0.0 0.0 0.6010747381480921 0.0 66-67 1.966224200680707E-4 0.0 0.0 0.726028286101351 0.0 68-69 1.966224200680707E-4 0.0 0.0 0.8795903961745142 0.0 70-71 1.966224200680707E-4 0.0 0.0 0.9796712079891621 0.0 72-73 1.966224200680707E-4 0.0 0.0 1.0987260833403791 0.0 74-75 1.966224200680707E-4 0.0 0.0 1.2500270355827592 0.0 76-77 1.966224200680707E-4 0.0 0.0 1.4055553698566032 0.0 78-79 1.966224200680707E-4 0.0 0.0 1.5867429299493305 0.0 80-81 1.966224200680707E-4 0.0 0.0 1.8496271055803408 0.0 82-83 1.966224200680707E-4 0.0 0.0 2.1313870335378864 0.0 84-85 1.966224200680707E-4 0.0 0.0 2.4713471978355805 0.0 86-87 1.966224200680707E-4 0.0 0.0 2.904309766825472 0.0 88-89 1.966224200680707E-4 0.0 0.0 3.42073855313426 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTACGC 25 0.005014284 57.01546 3 TACCTGG 505 0.0 31.988874 2 GTACCTG 530 0.0 31.391869 1 GTATAGG 275 0.0 29.386055 1 GCCGTAC 65 0.005819303 29.253084 1 ACCTGGG 545 0.0 27.897476 3 GTCTTAC 115 1.273762E-4 24.801527 1 GGTATCA 5085 0.0 24.399143 1 CATGGGC 530 0.0 24.204676 4 GTACATG 4555 0.0 23.89858 1 ATGGGCA 400 0.0 23.756441 5 TACATGG 4660 0.0 23.042728 2 GGTAGTT 145 2.3314622E-5 22.930489 7 GAGTACT 3960 0.0 22.78986 12-13 CCTTATA 230 3.603418E-9 22.723553 2 GTCTAAT 105 0.002184836 22.636316 1 ACATGGG 4615 0.0 22.546743 3 GTATCAA 6640 0.0 22.407907 1 TACACTG 390 0.0 21.929026 5 CATGGGT 455 0.0 21.929024 4 >>END_MODULE