FastQCFastQC Report
Thu 26 May 2016
SRR937720_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937720_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352742
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35050.9936440798090388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27760.7869774509414814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18860.5346683978658623No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.19135798969218296No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA6500.18427065674062063No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA5700.16159119129562116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.15393687170793383No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5110.14486508552993405No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4580.12983993967262192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.12417007331137205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG405.3591566E-447.5428772
TACCTGG2350.046.5313262
GTACCTG3700.032.164731
TTAGAGC753.0579112E-431.695253
CATTCCG753.0758145E-431.663739
ACCTGGG3500.031.242463
GTATCAA37450.028.4733031
GGTATCA26500.028.2030071
CCTAACG700.00834697327.1673582
CATAACG350.00839319327.1403476-77
ATGGGGA4950.024.9649145
ATGGGTA2101.2496457E-924.896335
CATGGGG9350.024.4035684
GGATATG1401.745184E-523.8019031
GTACATG31000.023.80191
TACATGG30950.023.6562292
GTGTAGC2052.5951522E-823.2213671
CATGGGT3700.023.1256774
GCCCTAG1652.3763987E-623.0806311
ATCAACG46200.022.7423733
Produced by FastQC (version 0.11.5)