##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937720_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 352742 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.902393250591082 33.0 31.0 34.0 30.0 34.0 2 31.99745706493698 33.0 31.0 34.0 30.0 34.0 3 32.0117933220314 34.0 31.0 34.0 30.0 34.0 4 35.626106332673736 37.0 35.0 37.0 35.0 37.0 5 35.63166847157412 37.0 35.0 37.0 35.0 37.0 6 35.71249525148692 37.0 35.0 37.0 35.0 37.0 7 35.663181588809955 37.0 35.0 37.0 35.0 37.0 8 35.6778211837547 37.0 35.0 37.0 35.0 37.0 9 37.22562949691276 39.0 37.0 39.0 34.0 39.0 10-11 37.18619274143708 39.0 37.0 39.0 34.0 39.0 12-13 37.09377958961507 39.0 37.0 39.0 34.0 39.0 14-15 38.20918830193172 40.0 38.0 41.0 33.5 41.0 16-17 38.14887651598052 40.0 38.0 41.0 33.0 41.0 18-19 38.16432406688175 40.0 38.0 41.0 33.5 41.0 20-21 38.217464889352556 40.0 38.0 41.0 34.0 41.0 22-23 38.20898276927613 40.0 38.0 41.0 34.0 41.0 24-25 38.1501961773761 40.0 38.0 41.0 34.0 41.0 26-27 38.03117150778756 40.0 38.0 41.0 33.5 41.0 28-29 37.87222247421629 40.0 38.0 41.0 33.0 41.0 30-31 37.57789545900403 40.0 37.0 41.0 33.0 41.0 32-33 37.37397729785509 40.0 36.0 41.0 33.0 41.0 34-35 37.31579029432277 40.0 36.0 41.0 33.0 41.0 36-37 37.13718241661044 40.0 36.0 41.0 32.0 41.0 38-39 36.94000289163185 40.0 36.0 41.0 31.5 41.0 40-41 36.68839123211866 39.5 35.5 41.0 31.0 41.0 42-43 36.381790373700895 39.0 35.0 41.0 30.0 41.0 44-45 35.9991509375124 39.0 35.0 41.0 29.5 41.0 46-47 35.43306297520567 39.0 35.0 41.0 26.5 41.0 48-49 34.934001338088464 39.0 35.0 40.5 24.0 41.0 50-51 34.01923785656372 38.0 34.0 39.5 22.0 40.5 52-53 34.1064758378645 38.0 34.5 40.0 22.0 40.5 54-55 34.38457994795063 39.0 35.0 40.0 20.5 41.0 56-57 34.187095667655115 38.5 34.0 40.0 18.5 41.0 58-59 33.943139745196206 38.0 34.0 40.0 17.5 41.0 60-61 33.61836838255722 38.0 34.0 40.0 13.0 41.0 62-63 33.25801577356822 38.0 33.0 40.0 8.0 41.0 64-65 32.83945489904803 37.0 33.0 40.0 7.5 41.0 66-67 32.33635064721524 36.5 32.0 39.5 2.0 41.0 68-69 31.883226267356882 36.0 31.5 39.0 2.0 40.5 70-71 31.440116288959068 35.5 31.0 39.0 2.0 40.0 72-73 30.97005601827965 35.0 31.0 38.0 2.0 40.0 74-75 30.43155479075358 35.0 30.0 37.5 2.0 39.5 76-77 29.896730471562783 34.5 29.5 37.0 2.0 39.0 78-79 29.398968651308888 34.0 29.0 36.0 2.0 39.0 80-81 28.861801259844306 34.0 29.0 36.0 2.0 38.0 82-83 28.334077030804384 34.0 27.5 35.0 2.0 37.0 84-85 27.801183301109592 34.0 26.5 35.0 2.0 37.0 86-87 27.246837632037014 33.5 26.0 35.0 2.0 36.0 88-89 26.718846919278114 33.0 25.0 35.0 2.0 36.0 90-91 26.152924800562452 32.5 24.5 35.0 2.0 35.0 92-93 25.538644391651687 32.0 21.0 34.5 2.0 35.0 94-95 24.825448911669152 32.0 18.0 34.0 2.0 35.0 96-97 23.997608733862144 31.0 4.5 34.0 2.0 35.0 98-99 22.896702122230977 31.0 2.0 34.0 2.0 35.0 100-101 19.773911243911982 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12010798803817835 1101 2 0.1688264882954158 1101 3 0.05328857919642971 1101 4 0.06192620873534338 1101 5 -0.04978042974835972 1101 6 0.04827451124673132 1101 7 0.04935535666039925 1101 8 0.012188686221655587 1101 9 0.3236046044720382 1101 10-11 0.2524044521500741 1101 12-13 0.26588291436036116 1101 14-15 0.32840332465410427 1101 16-17 0.38846109168164844 1101 18-19 0.2564779936102184 1101 20-21 0.26047097506064176 1101 22-23 0.4586855640611489 1101 24-25 0.3921227779470797 1101 26-27 0.5287475106369328 1101 28-29 0.3562646818122772 1101 30-31 0.2789946366581333 1101 32-33 0.4036316268966047 1101 34-35 0.6295297299055846 1101 36-37 0.7919014252229388 1101 38-39 0.8438873897569934 1101 40-41 0.9265843983459447 1101 42-43 0.7563817844124188 1101 44-45 0.8294125100395249 1101 46-47 0.9239487756066183 1101 48-49 1.187850156605208 1101 50-51 1.17240808153133 1101 52-53 1.2564779286633438 1101 54-55 1.552749650085623 1101 56-57 1.7768217043560313 1101 58-59 1.7136825257714605 1101 60-61 1.751042008126042 1101 62-63 1.8034500295249671 1101 64-65 1.6683066283356993 1101 66-67 1.446590769949676 1101 68-69 1.4612181898773464 1101 70-71 1.5618063553098303 1101 72-73 1.6437902860880698 1101 74-75 1.7677089759109883 1101 76-77 1.760567811518154 1101 78-79 1.828204177452701 1101 80-81 1.9692710541285585 1101 82-83 1.8647979904484338 1101 84-85 1.8363106027758818 1101 86-87 1.936192322762757 1101 88-89 2.215690869844355 1101 90-91 2.3630290253993884 1101 92-93 2.570651735857421 1101 94-95 2.8539583953939385 1101 96-97 2.621048412786969 1101 98-99 3.0981144323671685 1101 100-101 3.79245933365031 1102 1 -0.2021947010140117 1102 2 -0.1609109198488241 1102 3 -0.2860895545440947 1102 4 -0.20478585052217824 1102 5 -0.18310434197793057 1102 6 -0.40104606811443944 1102 7 -0.23032479074928602 1102 8 -0.3127623118035814 1102 9 -0.1345675301053717 1102 10-11 -0.13782286562498314 1102 12-13 -0.19178084766989656 1102 14-15 -0.15430007942315882 1102 16-17 0.014527389334688223 1102 18-19 -0.14070228226906778 1102 20-21 -0.1303610674591198 1102 22-23 -0.17701962390822246 1102 24-25 -0.19951550305630406 1102 26-27 -0.2139315576281362 1102 28-29 0.04733751306195444 1102 30-31 0.18251855769704406 1102 32-33 0.12143255775060169 1102 34-35 0.1940627612741892 1102 36-37 0.12264640206171151 1102 38-39 0.20866396277543942 1102 40-41 0.09500161620835712 1102 42-43 0.2776857141138578 1102 44-45 -0.05092998990541986 1102 46-47 0.32021783498893797 1102 48-49 0.27878093718648245 1102 50-51 0.40596580842698415 1102 52-53 0.37516731854287855 1102 54-55 0.12911106075519996 1102 56-57 0.10864582580750692 1102 58-59 0.1768915861815401 1102 60-61 0.355127157774767 1102 62-63 0.4139916940504733 1102 64-65 0.5604380490626184 1102 66-67 0.5831860686965449 1102 68-69 0.4566731190722457 1102 70-71 0.2649103642953605 1102 72-73 0.3208415784922103 1102 74-75 0.35725503776725986 1102 76-77 0.5555340056614106 1102 78-79 0.735340847080284 1102 80-81 0.8352919469555466 1102 82-83 0.6220390875053816 1102 84-85 0.6365367092661636 1102 86-87 0.9049989870313269 1102 88-89 1.2126617402809323 1102 90-91 1.2628759630512612 1102 92-93 1.1912736755280982 1102 94-95 1.5661277697102953 1102 96-97 1.3981435683820038 1102 98-99 1.8425957455466815 1102 100-101 2.405166628521073 1103 1 -0.09588009229540972 1103 2 -0.0740987965039075 1103 3 -0.08086147202088867 1103 4 -0.05571841551579837 1103 5 -0.009288743508747643 1103 6 0.14654553880061627 1103 7 0.03533526291718658 1103 8 0.029414259700402567 1103 9 -0.11882609694652757 1103 10-11 0.01078598494467542 1103 12-13 -0.004234109447928347 1103 14-15 0.08206665615947628 1103 16-17 0.04017402638216083 1103 18-19 -0.38850943486661293 1103 20-21 -0.2364425349293171 1103 22-23 -0.13353768135774402 1103 24-25 -0.2055408846601381 1103 26-27 -0.01853279811552966 1103 28-29 -0.23151041725959942 1103 30-31 -0.34843094302876665 1103 32-33 -0.037238757078064566 1103 34-35 -0.11397291373570795 1103 36-37 0.029818794618783784 1103 38-39 -0.1570510770345237 1103 40-41 -0.1396473522515862 1103 42-43 -0.12029519469712824 1103 44-45 0.1106555905283102 1103 46-47 0.20968139017303145 1103 48-49 0.29257146197423367 1103 50-51 0.1956152167760763 1103 52-53 0.2661605882389111 1103 54-55 0.20879588018070905 1103 56-57 0.007279970003175151 1103 58-59 0.06634634358267277 1103 60-61 0.246900130985523 1103 62-63 0.3899286579355774 1103 64-65 0.17262957679762536 1103 66-67 0.38099432101696706 1103 68-69 0.030648177357559092 1103 70-71 0.39406291785186554 1103 72-73 0.4172586813822825 1103 74-75 0.4634915661621051 1103 76-77 0.4012736792636602 1103 78-79 0.4400586581918837 1103 80-81 0.3558831579703714 1103 82-83 0.4201002760858614 1103 84-85 0.6262213087470947 1103 86-87 0.9537727960314584 1103 88-89 0.8398281943345118 1103 90-91 0.9291914515481601 1103 92-93 0.8535223888523404 1103 94-95 1.0414499885111574 1103 96-97 0.9943088347348805 1103 98-99 1.1273051987644678 1103 100-101 2.0125468687005466 1104 1 0.07064733576869031 1104 2 0.20208849154534647 1104 3 0.04019169577860637 1104 4 0.08770219664889822 1104 5 -0.04243529335083451 1104 6 0.11083447588126205 1104 7 0.2828646267374708 1104 8 0.26044166593266027 1104 9 0.3903010662246231 1104 10-11 0.11732098137881053 1104 12-13 0.18070337872649134 1104 14-15 0.23575841973523382 1104 16-17 0.13863415417117153 1104 18-19 0.11820167171640605 1104 20-21 0.12845769357723924 1104 22-23 -0.007479201144668934 1104 24-25 0.0027261041474133663 1104 26-27 -0.026574191277802583 1104 28-29 0.08845989144256805 1104 30-31 0.045397924481086704 1104 32-33 0.07185929697756421 1104 34-35 0.4904967909523137 1104 36-37 0.3021272930250589 1104 38-39 0.4553930698500679 1104 40-41 0.5136281509722451 1104 42-43 0.4876734256763413 1104 44-45 0.210022467265766 1104 46-47 0.1610735336227549 1104 48-49 0.26459760195997006 1104 50-51 0.4442303506052383 1104 52-53 0.2708937304223866 1104 54-55 0.4740250416926344 1104 56-57 0.7287188335833079 1104 58-59 0.6229941378394486 1104 60-61 0.7230277453479559 1104 62-63 0.5709597028072793 1104 64-65 0.6198794931089253 1104 66-67 0.8330763505044949 1104 68-69 1.010676413199615 1104 70-71 0.9000870493086275 1104 72-73 0.6895540806865768 1104 74-75 0.8786268507764845 1104 76-77 1.1239103829474182 1104 78-79 0.8221318093991847 1104 80-81 1.060485309134009 1104 82-83 1.1062487034658943 1104 84-85 1.400763931769493 1104 86-87 1.4654149867834931 1104 88-89 1.1465946082370273 1104 90-91 1.3011637396701836 1104 92-93 1.460788024086014 1104 94-95 1.109798191751004 1104 96-97 1.4521442809048501 1104 98-99 1.294898020456582 1104 100-101 1.9708800807458964 1105 1 -0.048673100499225797 1105 2 -0.052805973813548235 1105 3 -0.04758447981245695 1105 4 -0.1330156905291986 1105 5 -0.18935635285862418 1105 6 -0.061872649763508036 1105 7 -0.24913932173878806 1105 8 -0.08339029156315547 1105 9 -0.19811867228560232 1105 10-11 -0.13599844841476738 1105 12-13 -0.13920545792465333 1105 14-15 0.047610622849504125 1105 16-17 0.053403197343115494 1105 18-19 -0.038863971701346145 1105 20-21 -0.22689065299832833 1105 22-23 -0.13034891110586244 1105 24-25 -0.11580802404257895 1105 26-27 -0.08261739225331155 1105 28-29 -0.0076801826881407464 1105 30-31 -0.2941661947087013 1105 32-33 0.10987547798810482 1105 34-35 0.15014878309406754 1105 36-37 0.02734528181836282 1105 38-39 0.1049537050902245 1105 40-41 0.10380196134860853 1105 42-43 0.2740734411605672 1105 44-45 0.09972884773241475 1105 46-47 6.303945841281688E-4 1105 48-49 -0.13231619931941907 1105 50-51 -0.23878103997454758 1105 52-53 -0.1587909721354137 1105 54-55 -0.23714343630525292 1105 56-57 -0.3422521238254106 1105 58-59 -0.2221951083693412 1105 60-61 0.01142377364536884 1105 62-63 0.30409157997869585 1105 64-65 0.4294090631314731 1105 66-67 0.5011019188237817 1105 68-69 0.6459262414215416 1105 70-71 0.7735059652196341 1105 72-73 0.8269692803846311 1105 74-75 0.5964511242278192 1105 76-77 0.6444076199818021 1105 78-79 0.6299048881461253 1105 80-81 0.5197884224301177 1105 82-83 0.5009511644892051 1105 84-85 1.2417240502237838 1105 86-87 1.6709946289044453 1105 88-89 1.2049289899811306 1105 90-91 1.2308919915984546 1105 92-93 1.2851050247924825 1105 94-95 1.433285335009078 1105 96-97 1.3007255391913297 1105 98-99 1.7959286888910313 1105 100-101 2.620627010515282 1106 1 -0.10215348844875649 1106 2 -0.2287651189513049 1106 3 -0.41495208774310655 1106 4 0.016722529366091976 1106 5 -0.39839420368688394 1106 6 -0.47575986974462836 1106 7 -0.08641509205892817 1106 8 0.06594452948225182 1106 9 -0.08399971253965788 1106 10-11 -0.28053968950995056 1106 12-13 -0.08132201444340836 1106 14-15 -0.4719413469385003 1106 16-17 -0.22759414194106853 1106 18-19 -0.09443430901144723 1106 20-21 -0.0794269159800507 1106 22-23 -0.0924766254651459 1106 24-25 -0.35217829268904666 1106 26-27 -0.3267166112044748 1106 28-29 -0.17918048309154244 1106 30-31 -0.08159216672826375 1106 32-33 0.24072690704998223 1106 34-35 0.32805625884722645 1106 36-37 -0.047849980390893165 1106 38-39 -0.3056505348891747 1106 40-41 -0.2682390651727147 1106 42-43 -0.317377936403588 1106 44-45 -0.6240158952081032 1106 46-47 -2.5794118104727204E-4 1106 48-49 0.2953209133747805 1106 50-51 -0.14047617343948104 1106 52-53 0.054336656394333716 1106 54-55 0.13374747434619394 1106 56-57 0.2778234061720113 1106 58-59 0.027118255290432103 1106 60-61 0.09493162704851699 1106 62-63 0.0952468456211335 1106 64-65 0.07386876616125448 1106 66-67 0.08996553568070453 1106 68-69 0.15539323996243937 1106 70-71 -0.16033233192224117 1106 72-73 -0.2716531554577841 1106 74-75 -0.2607113510961838 1106 76-77 0.001389853266498875 1106 78-79 -0.07474707305892281 1106 80-81 -0.4257484413583974 1106 82-83 -0.12436855055671003 1106 84-85 -0.16976423404366514 1106 86-87 -0.5035526202587164 1106 88-89 -0.3998645899690878 1106 90-91 0.20338791469518114 1106 92-93 0.30333521962554144 1106 94-95 0.0651753015355574 1106 96-97 0.46680141250692486 1106 98-99 0.2234456402256768 1106 100-101 0.8663741566829906 1107 1 -0.2761425594870097 1107 2 -0.11357386212070253 1107 3 -0.15238378173217626 1107 4 -0.17327747063391286 1107 5 -0.17844884849562703 1107 6 -0.5358691593621145 1107 7 -0.3706227423321522 1107 8 -0.30525765631009705 1107 9 -0.5319232098074167 1107 10-11 -0.3225342250290737 1107 12-13 -0.4649558942248362 1107 14-15 -0.46347140158330546 1107 16-17 -0.5546159998645663 1107 18-19 -0.35727583906609084 1107 20-21 -0.23429030395818984 1107 22-23 -0.418760778470606 1107 24-25 -0.3775881287546241 1107 26-27 -0.3905144254121211 1107 28-29 -0.4033608109603932 1107 30-31 -0.34929708476105503 1107 32-33 -0.46960096180247746 1107 34-35 -0.06115139142599446 1107 36-37 -0.37339642847832266 1107 38-39 0.06210902795235285 1107 40-41 -0.22867622364265827 1107 42-43 -0.099809630392663 1107 44-45 -0.3500268241698521 1107 46-47 -0.32446559145427045 1107 48-49 -0.17618181886565765 1107 50-51 -0.6920881952974085 1107 52-53 -0.402002141419878 1107 54-55 -0.38668968412375904 1107 56-57 -0.24897987251650733 1107 58-59 -0.5731549687532862 1107 60-61 -0.32673503961814987 1107 62-63 -0.09650178825864941 1107 64-65 -0.285939977008141 1107 66-67 -0.05956998344498032 1107 68-69 -0.01479801686815918 1107 70-71 -0.00535965432661456 1107 72-73 -0.13329249972007773 1107 74-75 -0.2792905860688606 1107 76-77 -0.20656096640562893 1107 78-79 0.14131850071157004 1107 80-81 0.03602751492575251 1107 82-83 0.10013964616459958 1107 84-85 0.3166018861749187 1107 86-87 0.13251295351177106 1107 88-89 0.12999879800905134 1107 90-91 0.2554644174274223 1107 92-93 0.7580893179862009 1107 94-95 0.8492190173825485 1107 96-97 0.5871566037637521 1107 98-99 0.8414784271109284 1107 100-101 1.5008277085955584 1108 1 -0.0765189035730316 1108 2 0.07185624540618107 1108 3 0.11965922902050963 1108 4 0.07398059388221867 1108 5 0.1629490009667407 1108 6 0.004184604078744769 1108 7 0.22088263401192876 1108 8 0.1808176125071057 1108 9 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-2.9889928215236026 2316 98-99 -2.936107779785626 2316 100-101 -2.892792981059614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1506.0 3 250.0 4 226.0 5 223.0 6 265.0 7 336.0 8 394.0 9 551.0 10 646.0 11 905.0 12 1453.0 13 2054.0 14 2260.0 15 3097.0 16 4106.0 17 6366.0 18 5205.0 19 2828.0 20 2602.0 21 2575.0 22 2682.0 23 3018.0 24 3287.0 25 3704.0 26 4162.0 27 4916.0 28 5625.0 29 6808.0 30 8310.0 31 10080.0 32 13036.0 33 17122.0 34 23819.0 35 33658.0 36 48442.0 37 68601.0 38 52276.0 39 5348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56054786413789 20.36338392028998 15.07366120964851 21.002407005923622 2 14.234896695272381 21.25125825328852 42.42005470913733 22.093790342301762 3 18.3118016908714 28.001182984362426 31.160263100272147 22.526752224494032 4 11.038981349575325 17.52497291579585 39.55175286694476 31.884292867684067 5 11.175436900381595 39.630973436229326 35.096479205646006 14.097110457743076 6 27.337250534163747 38.345853980088194 19.267115970359594 15.049779515388462 7 25.72126653124423 35.269400684688264 21.93474153728142 17.074591246786085 8 25.341163835354884 35.025301386248884 20.87470912639896 18.75882565199726 9 26.96739034695171 16.632144815613536 22.00249466545059 34.39797017198416 10-11 23.36914752007001 28.13665363945158 27.819994289050747 20.674204551427668 12-13 24.812225548122395 26.38403302727791 28.750978472886178 20.052762951713518 14-15 22.181325893389854 26.826129202725202 28.526965059919196 22.465579843965745 16-17 19.04695616600639 30.334930404532972 29.435068615349213 21.183044814111422 18-19 18.861950977704613 28.142723426134904 33.5621059389501 19.433219657210383 20-21 19.89154148580779 27.04332659136877 33.63293411812831 19.432197804695132 22-23 19.47988119200049 27.170298568309626 33.33328598437769 20.016534255312198 24-25 19.04910036405888 27.49347662736308 33.767561970264495 19.689861038313538 26-27 19.086866964983308 27.40024149442432 33.80850912706869 19.70438241352369 28-29 18.510462676325467 27.358808211023707 34.42617594117204 19.704553171478782 30-31 18.983957219251337 27.44733387510158 34.05112890485148 19.517580000795604 32-33 18.602698505834166 27.71701064541656 33.75931101011407 19.920979838635205 34-35 18.900042904918724 27.566978556064797 33.72639163950776 19.806586899508723 36-37 19.158026911434874 27.349779052375457 33.499665489131594 19.99252854705808 38-39 18.95453706202332 27.886349756423183 33.47384567313838 19.685267508415116 40-41 18.8041556301972 27.788469049360536 33.792403121693496 19.614972198748767 42-43 18.744318569188465 27.93374883530668 33.18069222553008 20.141240369974774 44-45 18.68035403057072 28.338638678308598 32.88944570153422 20.09156158958647 46-47 19.15502641519083 28.19528631964441 32.14257810557837 20.507109159586392 48-49 19.144803953047614 28.67920132071344 31.779667037004412 20.396327689234536 50-51 19.42607428159838 28.681226727509596 31.49232336348487 20.400375627407165 52-53 19.221626229974067 29.174665198394372 31.297929025611882 20.30577954601968 54-55 19.11738383077863 28.96040799511315 31.495745315585356 20.426462858522864 56-57 19.37698376067769 29.401907250534325 30.720224950472552 20.50088403831543 58-59 19.291288272477892 28.860898604556063 31.25791575085334 20.589897372112702 60-61 19.426077719648042 28.97573320154735 31.413773907511235 20.184415171293377 62-63 19.52952269245024 28.759721735080323 31.59221323987782 20.118542332591616 64-65 19.310668029993185 28.649170643035678 31.577766416723474 20.46239491024767 66-67 19.33770545815002 29.061091402781763 30.912664487231563 20.68853865183666 68-69 19.100547584154143 28.981061767947015 31.318803040114517 20.59958760778433 70-71 19.27637614353189 28.892978763892085 31.118694421012872 20.711950671563155 72-73 19.227759306102566 28.777370785463603 30.92570315063182 21.069166757802012 74-75 19.15074641705609 29.03285371362016 30.941579194068435 20.874820675255314 76-77 19.315552411674215 28.755008785349133 31.130391058119667 20.799047744856985 78-79 19.019752942630248 28.501790363427443 31.210274314072784 21.268182379869526 80-81 19.046435459909045 28.956615355772264 30.779293085669973 21.21765609864872 82-83 19.279871623732568 28.858814507654294 30.69059047402653 21.17072339458661 84-85 18.996471248127346 28.469004494366068 30.638654388219503 21.89586986928708 86-87 19.122621654553047 28.629328879837995 30.919697912140037 21.328351553468927 88-89 19.432545746862626 28.061673758542916 30.972656982326175 21.533123512268283 90-91 19.002063589225024 28.051321307801448 31.442396319908877 21.504218783064648 92-93 19.015780683716017 28.490865608561787 31.150518638021335 21.34283506970086 94-95 18.520406786593256 28.484576505390162 31.031892216606334 21.963124491410248 96-97 18.719863197558233 28.540892312106138 30.63809419654841 22.10115029378722 98-99 18.688783974184165 28.52979274611399 30.3564732751568 22.424950004545043 100-101 19.051757311897124 27.95866117413512 31.201356376973965 21.78822513699379 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 640.0 1 410.5 2 233.5 3 261.0 4 210.0 5 207.5 6 350.0 7 670.0 8 1071.0 9 1221.5 10 1046.0 11 923.5 12 952.5 13 1001.0 14 1084.5 15 1153.5 16 1147.5 17 1167.0 18 1245.5 19 1284.0 20 1386.0 21 1531.5 22 1718.0 23 1968.5 24 2227.0 25 2675.0 26 3435.0 27 4116.0 28 4481.0 29 4889.0 30 5668.0 31 6615.5 32 7891.0 33 9481.5 34 10943.0 35 11932.0 36 13378.5 37 14894.0 38 14732.0 39 14123.5 40 13505.5 41 12770.0 42 12009.0 43 11983.0 44 12502.5 45 12440.0 46 11854.0 47 11395.0 48 12836.0 49 12705.5 50 10645.0 51 9291.0 52 7998.5 53 7163.5 54 6600.0 55 5962.0 56 5096.0 57 4368.5 58 3837.5 59 3271.0 60 2681.0 61 2168.0 62 1653.5 63 1184.0 64 809.0 65 542.0 66 383.5 67 280.5 68 201.5 69 146.5 70 109.0 71 78.5 72 59.0 73 48.5 74 38.0 75 24.5 76 14.0 77 9.0 78 8.5 79 6.5 80 4.0 81 3.0 82 2.0 83 1.5 84 1.5 85 0.5 86 0.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35946952730324144 2 0.30163689041849284 3 0.3087242233700552 4 0.3007864104643054 5 0.3005029171462429 6 0.22395972126936967 7 0.2146044417733074 8 0.22254225467905725 9 0.22424321458743215 10-11 0.22296749465615093 12-13 0.2241014679284009 14-15 0.2177228682719948 16-17 0.21077728197946374 18-19 0.2036899490279014 20-21 0.20354820236887014 22-23 0.21134426861558875 24-25 0.20935981538915127 26-27 0.21743937495393237 28-29 0.22693640110902588 30-31 0.22906260099449455 32-33 0.22863736101740081 34-35 0.22707814776805713 36-37 0.20850933543496378 38-39 0.1975948426895578 40-41 0.1954686428040891 42-43 0.20354820236887014 44-45 0.20935981538915127 46-47 0.2172976282949011 48-49 0.22991308094868204 50-51 0.2260859211548384 52-53 0.24224504028440053 54-55 0.22027430813455728 56-57 0.1883813098525268 58-59 0.16896201756524598 60-61 0.18597161664899559 62-63 0.1790260303564645 64-65 0.18767257655737055 66-67 0.18795606987543304 68-69 0.18427065674062063 70-71 0.1851211366948081 72-73 0.19887056262083902 74-75 0.20609964223143262 76-77 0.20850933543496378 78-79 0.2036899490279014 80-81 0.19986278923405776 82-83 0.1848376433767456 84-85 0.17987651031065197 86-87 0.18682209660318305 88-89 0.19731134937149533 90-91 0.19433466953183912 92-93 0.18880654982962053 94-95 0.18866480317058928 96-97 0.19887056262083902 98-99 0.20128025582437023 100-101 0.17789205708421452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 352742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.39119649248428 #Duplication Level Percentage of deduplicated Percentage of total 1 87.43003278736056 55.42294387767915 2 7.270817858596597 9.218116870707213 3 1.8771242129037553 3.569794494629456 4 0.8243237318577376 2.0901947063844704 5 0.480029775941831 1.5214830924485907 6 0.33153345923863753 1.26097815950577 7 0.239696382424786 1.0636248333779086 8 0.18329860535940953 0.929561432730933 9 0.14542962303787896 0.8297062028839893 >10 1.0888954565546305 13.325944654226602 >50 0.08887102833240129 3.826323320239601 >100 0.03635632977233599 3.778522731467213 >500 0.0022442178871812336 0.8390710493295792 >1k 0.0013465307323087402 2.323734574389513 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3505 0.9936440798090388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2776 0.7869774509414814 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1886 0.5346683978658623 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 675 0.19135798969218296 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 650 0.18427065674062063 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 570 0.16159119129562116 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 543 0.15393687170793383 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 511 0.14486508552993405 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 458 0.12983993967262192 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 438 0.12417007331137205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.834933180624933E-4 0.0 18-19 0.0 0.0 0.0 5.669866361249866E-4 0.0 20-21 0.0 0.0 0.0 8.504799541874798E-4 0.0 22-23 0.0 0.0 0.0 8.504799541874798E-4 0.0 24-25 0.0 0.0 0.0 0.0014174665903124663 0.0 26-27 0.0 0.0 0.0 0.0017009599083749596 0.0 28-29 0.0 0.0 0.0 0.002267946544499946 0.0 30-31 0.0 0.0 0.0 0.005244626384156126 0.0 32-33 0.0 0.0 0.0 0.010630999427343498 0.0 34-35 0.0 0.0 0.0 0.016159119129562114 0.0 36-37 0.0 0.0 0.0 0.021120252195655748 0.0 38-39 0.0 0.0 0.0 0.029341558419468054 0.0 40-41 0.0 0.0 0.0 0.038838584574561574 0.0 42-43 0.0 0.0 0.0 0.054714210386061196 0.0 44-45 0.0 0.0 0.0 0.06846363631209212 0.0 46-47 0.0 0.0 0.0 0.09893916800381014 0.0 48-49 0.0 0.0 0.0 0.13338360614840308 0.0 50-51 0.0 0.0 0.0 0.14996796525505893 0.0 52-53 0.0 0.0 0.0 0.18115223024193322 0.0 54-55 0.0 0.0 0.0 0.23501596067380692 0.0 56-57 0.0 0.0 0.0 0.32417460920446106 0.0 58-59 0.0 0.0 0.0 0.41475072432542764 0.0 60-61 0.0 0.0 0.0 0.5325421979803936 0.0 62-63 0.0 0.0 0.0 0.6491996983631096 0.0 64-65 0.0 0.0 0.0 0.7648649721326068 0.0 66-67 0.0 0.0 0.0 0.9172426305911969 0.0 68-69 0.0 0.0 0.0 1.1178141531204109 0.0 70-71 0.0 0.0 0.0 1.2565841323120015 0.0 72-73 0.0 0.0 0.0 1.431074269579466 0.0 74-75 0.0 0.0 0.0 1.6332050053580236 0.0 76-77 0.0 0.0 0.0 1.8225785418237692 0.0 78-79 0.0 0.0 0.0 2.057027515861451 0.0 80-81 0.0 0.0 0.0 2.416497043164693 0.0 82-83 0.0 0.0 0.0 2.7992130225490586 0.0 84-85 0.0 0.0 0.0 3.252235344812923 0.0 86-87 0.0 0.0 0.0 3.828719007093003 0.0 88-89 0.0 0.0 0.0 4.475650758911613 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 40 5.3591566E-4 47.542877 2 TACCTGG 235 0.0 46.531326 2 GTACCTG 370 0.0 32.16473 1 TTAGAGC 75 3.0579112E-4 31.69525 3 CATTCCG 75 3.0758145E-4 31.66373 9 ACCTGGG 350 0.0 31.24246 3 GTATCAA 3745 0.0 28.473303 1 GGTATCA 2650 0.0 28.203007 1 CCTAACG 70 0.008346973 27.167358 2 CATAACG 35 0.008393193 27.14034 76-77 ATGGGGA 495 0.0 24.964914 5 ATGGGTA 210 1.2496457E-9 24.89633 5 CATGGGG 935 0.0 24.403568 4 GGATATG 140 1.745184E-5 23.801903 1 GTACATG 3100 0.0 23.8019 1 TACATGG 3095 0.0 23.656229 2 GTGTAGC 205 2.5951522E-8 23.221367 1 CATGGGT 370 0.0 23.125677 4 GCCCTAG 165 2.3763987E-6 23.080631 1 ATCAACG 4620 0.0 22.742373 3 >>END_MODULE