FastQCFastQC Report
Thu 26 May 2016
SRR937720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352742
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37701.0687698090955997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.9686966678195396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.6585549778591718No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8090.22934609431255704No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA7920.22452670790549467No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7440.210919028638495No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.18455415005868311No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5730.16244167124980863No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5210.147700018710559No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5120.14514857884799656No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.11651575372368472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG2700.042.220772
GTATAGG1900.032.5081021
ACCTGGG3800.029.998973
GTACCTG3900.029.2380541
CACCTAC1951.6370905E-1129.2380547
GTATTGC700.00837363627.1496221
CCGTGCA700.00837363627.1496228
TGACCGT700.00838522927.1419265
ACCTACT1806.6465873E-926.3954668
GGTATCA39350.025.717971
CATGGGG9750.024.3581374
GAGTACT30100.023.91376712-13
ATGGGTA2002.0445441E-823.7491845
ATGGGGA4550.022.9662445
GTACATG33450.022.157991
GTATCAA50850.021.9572931
ACCCTTG2405.8753358E-921.7762587
GTACTTT33100.021.59669114-15
ACACCTA2205.6134013E-821.5901686
TACATGG34300.021.3257982
Produced by FastQC (version 0.11.5)