##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937720_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 352742 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29739866531346 33.0 31.0 34.0 31.0 34.0 2 32.52111174739611 34.0 31.0 34.0 31.0 34.0 3 32.594335236518475 34.0 31.0 34.0 31.0 34.0 4 36.196489218749115 37.0 35.0 37.0 35.0 37.0 5 36.05728832971407 37.0 35.0 37.0 35.0 37.0 6 36.17985099591203 37.0 36.0 37.0 35.0 37.0 7 36.14219741340697 37.0 36.0 37.0 35.0 37.0 8 36.152819908034765 37.0 36.0 37.0 35.0 37.0 9 37.8356986125837 39.0 38.0 39.0 35.0 39.0 10-11 37.78775564009956 39.0 37.5 39.0 35.0 39.0 12-13 37.70466374857544 39.0 37.0 39.0 35.0 39.0 14-15 38.87300349830755 40.0 38.0 41.0 35.0 41.0 16-17 38.84230825929433 40.0 38.0 41.0 35.5 41.0 18-19 38.9231974077371 40.0 38.0 41.0 35.0 41.0 20-21 38.88601867653979 40.0 38.0 41.0 35.0 41.0 22-23 38.75416593430893 40.0 38.0 41.0 35.0 41.0 24-25 38.690255200684916 40.0 38.0 41.0 34.5 41.0 26-27 38.57491877916438 40.0 38.0 41.0 34.0 41.0 28-29 38.45326754398398 40.0 38.0 41.0 34.0 41.0 30-31 38.454821087366966 40.0 38.0 41.0 35.0 41.0 32-33 38.269546864280414 40.0 37.0 41.0 34.0 41.0 34-35 38.11126829240635 40.0 37.0 41.0 34.0 41.0 36-37 37.921758679147935 40.0 37.0 41.0 34.0 41.0 38-39 37.75782158064534 40.0 37.0 41.0 33.0 41.0 40-41 37.54175147841765 40.0 37.0 41.0 33.0 41.0 42-43 37.271293183119674 40.0 36.5 41.0 33.0 41.0 44-45 36.824605519047914 40.0 36.0 41.0 31.5 41.0 46-47 36.24261499906447 40.0 36.0 41.0 30.5 41.0 48-49 35.722807320931445 40.0 36.0 41.0 28.0 41.0 50-51 35.41476631645792 39.0 35.0 41.0 26.5 41.0 52-53 35.172399374046755 39.0 35.0 41.0 25.5 41.0 54-55 34.97511920894024 39.0 35.0 40.0 24.5 41.0 56-57 34.72437078658056 39.0 35.0 40.0 23.5 41.0 58-59 34.485195978930776 38.5 35.0 40.0 23.0 41.0 60-61 34.14916851409812 38.0 34.0 40.0 22.0 41.0 62-63 33.78950479387201 38.0 34.0 40.0 20.5 41.0 64-65 33.418793339041 37.0 33.5 40.0 18.5 41.0 66-67 33.007692591185624 37.0 33.0 39.5 15.0 41.0 68-69 32.541458062833456 36.0 32.5 39.0 10.0 40.0 70-71 32.148764536119884 36.0 32.0 39.0 7.5 40.0 72-73 31.70346031944027 35.0 32.0 38.0 6.0 40.0 74-75 31.19450901792245 35.0 31.0 37.0 2.0 39.5 76-77 29.96720265803335 34.0 29.5 36.0 2.0 38.5 78-79 30.472763379467146 34.5 31.0 36.0 2.0 39.0 80-81 30.262548831724036 34.5 31.0 36.0 2.0 38.5 82-83 29.911171621184888 34.0 30.5 36.0 2.0 37.0 84-85 29.510044168258954 34.0 30.0 35.0 2.0 37.0 86-87 29.08632088041685 34.0 29.5 35.0 2.0 36.0 88-89 28.655944004399814 34.0 29.0 35.0 2.0 36.0 90-91 28.169676420726763 34.0 29.0 35.0 2.0 35.5 92-93 27.75386543139178 34.0 28.0 35.0 2.0 35.0 94-95 27.311349371495314 33.0 27.0 35.0 2.0 35.0 96-97 26.814686087848912 33.0 26.0 35.0 2.0 35.0 98-99 26.09553866565365 33.0 24.5 35.0 2.0 35.0 100-101 23.784868260655095 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11859371493488169 1101 2 0.09889569083202332 1101 3 0.10921276632039678 1101 4 0.14912342207223617 1101 5 -0.14361359727360679 1101 6 -0.07049981512687253 1101 7 -0.026380288255971607 1101 8 0.021647967004383872 1101 9 0.20266221957003694 1101 10-11 0.10682869819129337 1101 12-13 0.08650289868265304 1101 14-15 0.21760410240813854 1101 16-17 0.10979056049590952 1101 18-19 0.26505022064836936 1101 20-21 0.2693878391865425 1101 22-23 0.12953734835738118 1101 24-25 -0.008915348281099966 1101 26-27 0.14325555313726568 1101 28-29 0.13307875936479263 1101 30-31 0.4478692466578309 1101 32-33 0.4915197088037502 1101 34-35 0.3832768587615405 1101 36-37 0.3169557365574107 1101 38-39 0.4096493937087544 1101 40-41 0.4156198606188397 1101 42-43 0.4262279648192546 1101 44-45 0.7675651583841372 1101 46-47 0.5217506150641995 1101 48-49 0.7663758636455498 1101 50-51 1.1502030575693567 1101 52-53 1.232174476002939 1101 54-55 1.103490050068487 1101 56-57 1.1070996094432672 1101 58-59 1.3017971352328814 1101 60-61 1.3658713677892962 1101 62-63 1.5310191764075824 1101 64-65 1.5793348342774038 1101 66-67 1.4522170930763494 1101 68-69 1.3796293806176152 1101 70-71 1.5969407674484444 1101 72-73 1.595056092185768 1101 74-75 1.6113584034004198 1101 76-77 1.50764962366328 1101 78-79 1.500310247655225 1101 80-81 1.6017282617516067 1101 82-83 1.424982023045331 1101 84-85 1.835361398972836 1101 86-87 2.051825452494974 1101 88-89 2.0440266142236183 1101 90-91 2.224021064727509 1101 92-93 1.98890785491081 1101 94-95 1.9010681458488001 1101 96-97 2.0544289898278763 1101 98-99 2.257232453018272 1101 100-101 2.984436967492897 1102 1 -0.01749229362486915 1102 2 -0.07779955726111609 1102 3 -0.06004959796064924 1102 4 -0.06395808638105649 1102 5 -0.36365999315083286 1102 6 -0.21621003176331755 1102 7 -0.23937003271159085 1102 8 -0.16157720716368118 1102 9 -0.06894086076383843 1102 10-11 -0.1292039037218089 1102 12-13 -0.19203662223586804 1102 14-15 -0.2629859768025469 1102 16-17 -0.21921732386677206 1102 18-19 -0.0667188424045051 1102 20-21 -0.04883135196887878 1102 22-23 -0.06401035790973708 1102 24-25 -0.22401566468336398 1102 26-27 -0.24687265952029236 1102 28-29 -0.18040173895042244 1102 30-31 0.042346705749437774 1102 32-33 -0.22065241843253602 1102 34-35 -0.5376996569961747 1102 36-37 -0.4298169236441822 1102 38-39 -0.14762143488344037 1102 40-41 -0.30076606939092443 1102 42-43 -0.15317542237342963 1102 44-45 -0.13691494351061095 1102 46-47 -0.4308551154139266 1102 48-49 -0.6026053357466026 1102 50-51 -0.564599025840451 1102 52-53 -0.47131402413286594 1102 54-55 -0.3281641244592919 1102 56-57 -0.30960620986240883 1102 58-59 -0.3502732440827785 1102 60-61 -0.08887888731461402 1102 62-63 -0.013430386428730401 1102 64-65 -0.07584175635973622 1102 66-67 0.04590354650795092 1102 68-69 -0.15172913030963997 1102 70-71 0.019881032756515538 1102 72-73 -0.14383128769515352 1102 74-75 -0.06656097642177983 1102 76-77 -0.24174748687196157 1102 78-79 0.008962674996492836 1102 80-81 0.21016302489037741 1102 82-83 0.40816535283045496 1102 84-85 0.25967170076627255 1102 86-87 0.10082559197625329 1102 88-89 0.2547819053357223 1102 90-91 0.19177703122263878 1102 92-93 0.3420946350222458 1102 94-95 -0.1577901999032605 1102 96-97 0.43364003968141773 1102 98-99 0.5411750381202012 1102 100-101 0.9609940190055362 1103 1 0.03697917956388608 1103 2 0.1041891815766931 1103 3 0.12379987197292763 1103 4 0.06127175569873344 1103 5 -0.17156480113921901 1103 6 -0.24202712585560704 1103 7 -0.041501295633338486 1103 8 -0.01666672460528673 1103 9 -0.031211568545479906 1103 10-11 -0.08907391647859697 1103 12-13 -0.14942785964403527 1103 14-15 -0.019225804366001853 1103 16-17 0.06365669188126333 1103 18-19 0.019805153548510646 1103 20-21 -0.04709378983453405 1103 22-23 -0.08924129971239836 1103 24-25 -0.0810934810736299 1103 26-27 -0.010176007076708515 1103 28-29 -0.19537116665463827 1103 30-31 -0.0669206588746789 1103 32-33 -0.01141364660303168 1103 34-35 -0.08439828894092472 1103 36-37 -0.19222509678454713 1103 38-39 -0.25478612938042744 1103 40-41 -0.29463291494584354 1103 42-43 -0.346508902336474 1103 44-45 -0.6500753270939512 1103 46-47 -0.6297202014122618 1103 48-49 -0.2943068269708107 1103 50-51 -0.07536421301328033 1103 52-53 -0.1428707918274128 1103 54-55 -0.08545528708630457 1103 56-57 -0.07323397566542411 1103 58-59 0.13365430617456298 1103 60-61 0.016763790730522032 1103 62-63 0.23178691881035007 1103 64-65 0.27146392260917196 1103 66-67 0.5180794581782138 1103 68-69 0.2502316755672851 1103 70-71 0.3204220964084783 1103 72-73 0.43092787299635305 1103 74-75 0.19010994250664837 1103 76-77 0.2720727304332762 1103 78-79 0.27077699778715214 1103 80-81 0.5798227706344505 1103 82-83 0.37173153415276516 1103 84-85 0.44631776595784345 1103 86-87 0.5841647904982246 1103 88-89 0.7977623742575553 1103 90-91 0.6831662011518169 1103 92-93 0.8498036923398047 1103 94-95 0.6324079701578817 1103 96-97 0.8250468051765161 1103 98-99 0.8406152791794561 1103 100-101 1.2707386633551607 1104 1 0.06658023913569622 1104 2 0.13512343863929033 1104 3 0.12329337336288404 1104 4 0.10375068797637965 1104 5 -0.031340369858568806 1104 6 -0.059708002622166134 1104 7 -0.015501033651297291 1104 8 0.042119134186393126 1104 9 0.0873236607615766 1104 10-11 0.06175351013004615 1104 12-13 0.07159037721683603 1104 14-15 0.18556264513696163 1104 16-17 0.04210430275060162 1104 18-19 0.08091537521442405 1104 20-21 0.27674512032017873 1104 22-23 0.24054023637562238 1104 24-25 0.15733637323524619 1104 26-27 0.08275047785204492 1104 28-29 0.09027949485001585 1104 30-31 0.009569363975984402 1104 32-33 -0.07179322946940658 1104 34-35 -0.13139641140296732 1104 36-37 0.021345572870650642 1104 38-39 -0.10149461614305721 1104 40-41 -0.15411157068688652 1104 42-43 -0.032906212654204126 1104 44-45 -0.15988218811247634 1104 46-47 0.06623799789785778 1104 48-49 0.1579585899444993 1104 50-51 0.27145488838831966 1104 52-53 0.5068301120106256 1104 54-55 0.24821522683512853 1104 56-57 0.24873030744982572 1104 58-59 0.038329230126997516 1104 60-61 0.31946511665073274 1104 62-63 0.4650222514547693 1104 64-65 0.09223618744390194 1104 66-67 0.21900658350751456 1104 68-69 0.24050049824909792 1104 70-71 0.2606931022739616 1104 72-73 0.30107210866374956 1104 74-75 0.1808445110367849 1104 76-77 0.050367518446140735 1104 78-79 0.36713835834720854 1104 80-81 0.39883830034758105 1104 82-83 0.2686270469826084 1104 84-85 0.13439030587371903 1104 86-87 0.20364315514811437 1104 88-89 0.3910454458514252 1104 90-91 0.6092508795688651 1104 92-93 0.31963209410381666 1104 94-95 0.3792416261172491 1104 96-97 0.6849824740312194 1104 98-99 0.46648018377432976 1104 100-101 1.2134271812645423 1105 1 -0.08843616446051783 1105 2 -0.03814499000264249 1105 3 0.05547732590375176 1105 4 -0.015835883333295442 1105 5 -0.08431575962543292 1105 6 -0.06698074749059657 1105 7 -0.15382351190080357 1105 8 -0.23435646364968932 1105 9 -0.021206209267745635 1105 10-11 -0.0026390729282752545 1105 12-13 -0.29875588866659086 1105 14-15 -0.13212517362023135 1105 16-17 -0.03531680030656048 1105 18-19 -0.05828776808235858 1105 20-21 -0.07940405079013857 1105 22-23 -0.1161799153697416 1105 24-25 -0.3487048922126874 1105 26-27 -0.2600750053040173 1105 28-29 -0.15126224964099322 1105 30-31 -0.21785028743551038 1105 32-33 0.028554197730557007 1105 34-35 0.126856194301034 1105 36-37 0.2612130860080484 1105 38-39 -0.2072349070528503 1105 40-41 -0.06113124532470238 1105 42-43 0.0350283858689906 1105 44-45 -0.03495598010132284 1105 46-47 0.30360626105218813 1105 48-49 0.25941168727249675 1105 50-51 0.20982769035482107 1105 52-53 0.3769997610027005 1105 54-55 0.2724310962257235 1105 56-57 0.31175592415730335 1105 58-59 0.23847971739606777 1105 60-61 0.39013848669456763 1105 62-63 0.20137920145040766 1105 64-65 0.36285197554234117 1105 66-67 -0.07479682495655027 1105 68-69 -0.13948469496494198 1105 70-71 0.09102233551470817 1105 72-73 0.1039603996896794 1105 74-75 0.15484795627586934 1105 76-77 -0.19147181340973773 1105 78-79 -0.07051829841600821 1105 80-81 0.013670732047650347 1105 82-83 0.11774299476207162 1105 84-85 0.09432246563216395 1105 86-87 0.17607640364082044 1105 88-89 0.2176391108696656 1105 90-91 0.17900245460442932 1105 92-93 0.668237546484999 1105 94-95 0.7301818086375 1105 96-97 0.8940000970098012 1105 98-99 0.4090821134953231 1105 100-101 0.7238161255310267 1106 1 -0.10774781152943547 1106 2 0.02106022843354083 1106 3 -0.1524619067801396 1106 4 -0.16761684634076346 1106 5 -0.2030866805764262 1106 6 -0.19726141009466858 1106 7 -0.2764567622655818 1106 8 -0.023327367434767154 1106 9 -0.1271971113362369 1106 10-11 -0.15948827042205238 1106 12-13 -0.3304398642909945 1106 14-15 -0.31235862997591113 1106 16-17 -0.13110256976840873 1106 18-19 0.12437466432687927 1106 20-21 -0.020761015857509335 1106 22-23 -0.2420875627819754 1106 24-25 -4.558148802615847E-4 1106 26-27 -0.14536382171462492 1106 28-29 -0.32937142481908666 1106 30-31 -0.3040059822256538 1106 32-33 -0.11582825871094826 1106 34-35 -0.5114151987124416 1106 36-37 -0.6001507454227308 1106 38-39 -0.4230060854924673 1106 40-41 -1.0051918409817304 1106 42-43 -0.7746746439138619 1106 44-45 -0.6515339562407192 1106 46-47 -0.6645853104867356 1106 48-49 -1.072195041763635 1106 50-51 -1.8287784378745187 1106 52-53 -1.7965819058070167 1106 54-55 -1.6449880094304419 1106 56-57 -1.3988955220704824 1106 58-59 -1.511563197375203 1106 60-61 -1.4639384165755303 1106 62-63 -1.8052725880955478 1106 64-65 -2.1412506765905235 1106 66-67 -1.4918082402857067 1106 68-69 -2.1656841628217762 1106 70-71 -1.8447127945170436 1106 72-73 -1.0968764383499767 1106 74-75 -1.4602966665032895 1106 76-77 -1.6216151487457644 1106 78-79 -1.5400917255896402 1106 80-81 -1.690255969523033 1106 82-83 -1.453529857353626 1106 84-85 -1.4994935158286538 1106 86-87 -1.4433039070621234 1106 88-89 -1.5011275186041964 1106 90-91 -1.1729587504423478 1106 92-93 -1.0412509094415299 1106 94-95 -0.7474939273508276 1106 96-97 -0.7905334194265556 1106 98-99 -1.2542343400279776 1106 100-101 -0.5028511133325893 1107 1 -0.0383489044256109 1107 2 -0.07746436173039228 1107 3 -0.09541272645227394 1107 4 0.08615364546251669 1107 5 -0.46576427620483685 1107 6 -0.12431059042253878 1107 7 -0.06525457647322952 1107 8 -0.2074803728992478 1107 9 -0.10583099111765648 1107 10-11 -0.09763034692478811 1107 12-13 -0.1427349462582157 1107 14-15 -0.24845152615078092 1107 16-17 -0.4125506572001072 1107 18-19 -0.21669090944361358 1107 20-21 -0.08128014154056729 1107 22-23 -0.19812581414809927 1107 24-25 -0.2153192028584101 1107 26-27 -0.31659332991134903 1107 28-29 -0.1274315341087089 1107 30-31 -0.04203876911090276 1107 32-33 -0.18536377783662772 1107 34-35 -0.058519023849051166 1107 36-37 0.2244940633204422 1107 38-39 -0.05355253357989653 1107 40-41 0.058004880329747266 1107 42-43 0.11073519215171501 1107 44-45 0.02218189075382071 1107 46-47 -0.13734487332826717 1107 48-49 -0.2121677193592575 1107 50-51 -0.32705712639911155 1107 52-53 -0.6706529440583893 1107 54-55 -0.6978568618894627 1107 56-57 -0.7670922433819598 1107 58-59 -0.5913719405445974 1107 60-61 -0.2424356843350992 1107 62-63 -0.38729444601904106 1107 64-65 -0.5216331902517197 1107 66-67 -0.17732736596876464 1107 68-69 -0.2230885344064788 1107 70-71 -0.2702865650088455 1107 72-73 -0.23398026348658973 1107 74-75 -0.2433021309841621 1107 76-77 -0.15694301759821983 1107 78-79 -0.28402615181914825 1107 80-81 -0.3835893028563646 1107 82-83 -0.20355717975800047 1107 84-85 -0.12422029178493688 1107 86-87 -0.3255989890293378 1107 88-89 -0.2409909065823399 1107 90-91 -0.08238497995054672 1107 92-93 0.3515359758043708 1107 94-95 0.6070142693704845 1107 96-97 0.5102042854914792 1107 98-99 0.5016126545075394 1107 100-101 1.2279958812029292 1108 1 0.1839629235313751 1108 2 0.16156789633412671 1108 3 0.21087759612837687 1108 4 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84-85 -0.3308294871872377 2316 86-87 -0.38893232236266684 2316 88-89 -0.6823127456628022 2316 90-91 -0.42462635926088765 2316 92-93 -0.3906479322416061 2316 94-95 -0.5059167651122749 2316 96-97 -0.2468646800257659 2316 98-99 -0.402180448940733 2316 100-101 -0.8410271073028177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 2.0 6 12.0 7 24.0 8 91.0 9 161.0 10 319.0 11 555.0 12 968.0 13 1476.0 14 1866.0 15 2747.0 16 4116.0 17 6552.0 18 5759.0 19 2839.0 20 2006.0 21 1856.0 22 1873.0 23 2019.0 24 2167.0 25 2499.0 26 2907.0 27 3448.0 28 4405.0 29 5251.0 30 6635.0 31 8350.0 32 10914.0 33 14734.0 34 20678.0 35 30779.0 36 48559.0 37 79024.0 38 70137.0 39 7011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.161708065549966 20.95517122063837 15.522737715053916 19.36038299875775 2 15.255342431578889 22.40050802002597 41.5949901060832 20.749159442311946 3 19.548565240317288 28.428709935306824 31.08759376541495 20.93513105896094 4 12.052151430790776 18.5333756683355 38.87033582618458 30.544137074689147 5 11.532845473593481 40.493398015860635 34.315006932295994 13.658749578249886 6 28.315029114763764 38.76941220495433 18.111254117740444 14.804304562541462 7 26.117672406461377 36.17714930459089 21.28070941367912 16.424468875268612 8 26.466369187678247 35.48627608847259 19.945455885604776 18.10189883824438 9 27.949889721099275 17.07792097340266 21.541806759614676 33.43038254588339 10-11 24.31550538353811 28.845870352835785 26.78728362372499 20.051340639901117 12-13 25.97665857243908 26.782820958804493 28.295633511519746 18.94488695723668 14-15 23.089113289599762 27.22726525335798 28.295468075817453 21.388153381224807 16-17 19.6130599701765 30.7512289435338 29.307397474641522 20.328313611648174 18-19 19.54261188063797 28.30042920888355 33.61408621598789 18.54287269449059 20-21 20.6135371086192 27.149626567897457 33.8594976774777 18.377338646005644 22-23 20.3281741908689 27.11320960652266 33.737786666931264 18.820829535677177 24-25 20.01326752489787 27.51693168642148 34.09116036978973 18.378640418890914 26-27 19.901739405305108 27.451730829474208 34.270721262815904 18.375808502404777 28-29 19.27063916526216 27.475700493432008 34.675045041086264 18.57861530021957 30-31 19.704344818592627 27.766469544312837 34.186459225156064 18.34272641193847 32-33 19.3912264487926 27.951307187689583 34.02883694031332 18.628629423204497 34-35 19.628935595988004 27.664553696469373 34.22586479636675 18.480645911175873 36-37 20.01973113493715 27.527201183868094 33.88836600121335 18.5647016799814 38-39 19.829223625199155 27.932880122015526 34.05179989907638 18.186096353708944 40-41 19.82086057231631 27.941526668216433 33.97780814306206 18.25980461640519 42-43 19.53873568383732 28.11461152324396 33.52990959854496 18.81674319437376 44-45 19.739072750055282 28.675065628703134 32.86424638971259 18.721615231528993 46-47 20.14049928843177 28.723684732750847 32.17762557336524 18.958190405452143 48-49 20.169557353533175 29.080319326873465 31.633743642662342 19.116379676931015 50-51 20.479699043493547 29.184928361238526 31.200565852662855 19.134806742605075 52-53 20.4736039371552 29.479477918705456 31.099358738114542 18.9475594060248 54-55 20.092588917679212 29.512930130236832 31.072001632921513 19.322479319162447 56-57 20.62456412902348 29.85694927170567 30.322728793282344 19.195757805988514 58-59 20.50166977564339 29.394713416604773 30.756473569917958 19.347143237833883 60-61 20.489621309625733 29.530364969297672 30.962147972172293 19.0178657489043 62-63 20.525341467701605 29.19839429384649 31.432463386837973 18.843800851613928 64-65 20.369703636085298 29.259487103888958 31.011901049492263 19.358908210533478 66-67 20.440293472282857 29.42320449507005 30.66462173486571 19.471880297781382 68-69 20.2563063088603 29.560131767694237 30.77192395575236 19.411637967693103 70-71 20.333558238032328 29.68600280091398 30.640241309512334 19.340197651541352 72-73 20.43674980580708 29.3767115909078 30.38495557659706 19.80158302668806 74-75 20.391107381599017 29.75290722397673 30.141293069722348 19.714692324701907 76-77 20.320659292060487 29.46190133298558 30.719619438569833 19.4978199363841 78-79 20.305350652885114 29.06472719438003 30.523867302447684 20.106054850287176 80-81 20.226964750440832 29.68118341450692 30.271983489349157 19.819868345703092 82-83 20.51641142818264 29.623492524281204 30.04844900805688 19.81164703947928 84-85 20.145176928179804 29.229578558833367 30.102170991829723 20.523073521157105 86-87 20.062396879305556 29.272811289837897 30.260785503285685 20.40400632757086 88-89 20.424134353153296 28.727511892544694 30.47822487823962 20.37012887606239 90-91 19.969465735423576 29.05135398341737 30.83124361211561 20.147936669043446 92-93 19.968588450585635 29.431175962793755 30.512436337039123 20.087799249581487 94-95 19.53325660114191 29.458357666509798 30.362843097788183 20.645542634560105 96-97 19.996484682856025 29.318028474068864 29.82973391317167 20.855752929903442 98-99 19.779668368576377 29.45163732030016 29.83520486702708 20.933489444096377 100-101 19.84252995446581 29.44567935537649 29.86331237135032 20.848478318807377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 407.0 1 279.0 2 242.0 3 309.0 4 222.0 5 199.5 6 364.5 7 708.5 8 1071.0 9 1250.0 10 1156.5 11 992.5 12 997.0 13 1042.5 14 1090.0 15 1133.0 16 1188.0 17 1238.5 18 1303.5 19 1433.0 20 1539.5 21 1675.0 22 1864.5 23 2188.5 24 2495.0 25 2871.0 26 3560.5 27 4253.0 28 4614.0 29 4972.5 30 5734.0 31 6539.0 32 7681.0 33 9265.5 34 10798.0 35 11901.0 36 13283.5 37 14415.0 38 14511.5 39 14275.5 40 13504.0 41 12775.5 42 11951.0 43 11942.0 44 12545.0 45 12569.5 46 12087.5 47 11547.0 48 13026.5 49 12740.0 50 10732.5 51 9253.5 52 7624.0 53 7026.0 54 6521.5 55 5803.0 56 4952.0 57 4189.0 58 3670.0 59 3241.0 60 2705.0 61 2130.0 62 1586.0 63 1121.0 64 768.5 65 515.0 66 362.0 67 276.5 68 197.5 69 147.0 70 102.0 71 69.5 72 61.0 73 45.0 74 31.0 75 24.0 76 12.5 77 7.0 78 8.5 79 6.5 80 2.5 81 0.5 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04422495761774895 2 0.0 3 0.0 4 0.0 5 0.012757199312812197 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006095106338343605 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.4174665903124664E-4 22-23 7.087332951562331E-4 24-25 2.834933180624933E-4 26-27 0.0018427065674062064 28-29 0.002409693203531193 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.010630999427343498 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.006662092974468591 92-93 0.001559213249343713 94-95 0.0 96-97 0.0 98-99 2.834933180624933E-4 100-101 0.01133973272249973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 352742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.25328496532264 #Duplication Level Percentage of deduplicated Percentage of total 1 86.45336815045839 52.95552795531976 2 7.822042658936964 9.582516159975519 3 2.072105681443021 3.807698393510803 4 0.9237970620343874 2.2634241876368066 5 0.5371376728778198 1.6450723471197672 6 0.36579596864660013 1.3443722824005855 7 0.24982272678755493 1.071172387231243 8 0.1866425342146314 0.9145974667919037 9 0.1462416619036029 0.8062003971345381 >10 1.0923327070458462 12.96686458229011 >50 0.09954512218937009 4.153969764980618 >100 0.0465164122380193 4.467675829267553 >500 0.0032561488566613507 1.311240061625592 >1k 0.001395492367140579 2.709668184715206 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3770 1.0687698090955997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.9686966678195396 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2323 0.6585549778591718 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 809 0.22934609431255704 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 792 0.22452670790549467 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 744 0.210919028638495 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 651 0.18455415005868311 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 573 0.16244167124980863 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 521 0.147700018710559 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 512 0.14514857884799656 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 411 0.11651575372368472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.834933180624933E-4 0.0 18-19 0.0 0.0 0.0 5.669866361249866E-4 0.0 20-21 0.0 0.0 0.0 8.504799541874798E-4 0.0 22-23 0.0 0.0 0.0 8.504799541874798E-4 0.0 24-25 0.0 0.0 0.0 0.0014174665903124663 0.0 26-27 0.0 0.0 0.0 0.0017009599083749596 0.0 28-29 0.0 0.0 0.0 0.002267946544499946 0.0 30-31 0.0 0.0 0.0 0.005244626384156126 0.0 32-33 0.0 0.0 0.0 0.010630999427343498 0.0 34-35 0.0 0.0 0.0 0.01644261244762461 0.0 36-37 0.0 0.0 0.0 0.021261998854686992 0.0 38-39 0.0 0.0 0.0 0.029341558419468054 0.0 40-41 0.0 0.0 0.0 0.038838584574561574 0.0 42-43 0.0 0.0 0.0 0.05443071706799871 0.0 44-45 0.0 0.0 0.0 0.06803839633499839 0.0 46-47 0.0 0.0 0.0 0.09865567468574765 0.0 48-49 0.0 0.0 0.0 0.13366709946646557 0.0 50-51 0.0 0.0 0.0 0.15010971191409017 0.0 52-53 0.0 0.0 0.0 0.18285319015030815 0.0 54-55 0.0 0.0 0.0 0.2372839072183069 0.0 56-57 0.0 0.0 0.0 0.3280017689983047 0.0 58-59 0.0 0.0 0.0 0.42013709736861504 0.0 60-61 0.0 0.0 0.0 0.5406217575451746 0.0 62-63 0.0 0.0 0.0 0.6602559377675468 0.0 64-65 0.0 0.0 0.0 0.7757794648780129 0.0 66-67 0.0 0.0 0.0 0.930425069881103 0.0 68-69 0.0 0.0 0.0 1.133406285613848 0.0 70-71 0.0 0.0 0.0 1.2706170515560948 0.0 72-73 0.0 0.0 0.0 1.444823695505497 0.0 74-75 0.0 0.0 0.0 1.6502146044417731 0.0 76-77 0.0 0.0 0.0 1.8429900607242686 0.0 78-79 0.0 0.0 0.0 2.0797069813064506 0.0 80-81 0.0 0.0 0.0 2.4421531884493484 0.0 82-83 0.0 0.0 0.0 2.8272788610372457 0.0 84-85 0.0 0.0 0.0 3.2859710496623595 0.0 86-87 0.0 0.0 0.0 3.8699672848710955 0.0 88-89 0.0 0.0 0.0 4.529514489343486 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 270 0.0 42.22077 2 GTATAGG 190 0.0 32.508102 1 ACCTGGG 380 0.0 29.99897 3 GTACCTG 390 0.0 29.238054 1 CACCTAC 195 1.6370905E-11 29.238054 7 GTATTGC 70 0.008373636 27.149622 1 CCGTGCA 70 0.008373636 27.149622 8 TGACCGT 70 0.008385229 27.141926 5 ACCTACT 180 6.6465873E-9 26.395466 8 GGTATCA 3935 0.0 25.71797 1 CATGGGG 975 0.0 24.358137 4 GAGTACT 3010 0.0 23.913767 12-13 ATGGGTA 200 2.0445441E-8 23.749184 5 ATGGGGA 455 0.0 22.966244 5 GTACATG 3345 0.0 22.15799 1 GTATCAA 5085 0.0 21.957293 1 ACCCTTG 240 5.8753358E-9 21.776258 7 GTACTTT 3310 0.0 21.596691 14-15 ACACCTA 220 5.6134013E-8 21.590168 6 TACATGG 3430 0.0 21.325798 2 >>END_MODULE