FastQCFastQC Report
Thu 26 May 2016
SRR937719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200278
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109730.49870970850047125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82930.37690691812580046No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75470.3430021115513585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.25364976607501416No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50390.22901651518580832No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39800.18088623346686192No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32780.14898117419707874No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30910.14048224815227894No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23330.10603205594929369No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23190.10539577271599315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98350.026.4600071
TACATGG97900.026.1359232
ACATGGG100300.024.7981453
GAGTACT92450.023.88967312-13
GTATCAA196350.023.8419171
CATGGGA57950.022.5697864
GTACTTT98800.022.18498414-15
AGTACTT96300.022.09611112-13
AGAGTAC147300.021.36226510-11
TCAACGC223200.020.775844
ATCAACG223350.020.7604663
CAACGCA223650.020.6915075
TATCAAC224400.020.6430762
AACGCAG227200.020.3854276
GGTATCA148800.019.5990281
ACTTTTT119050.018.80948316-17
CGCAGAG245800.018.6684748
ACGCAGA246950.018.6589077
GTGTAGC16000.018.4352341
TACTTTT113500.018.43293214-15
Produced by FastQC (version 0.11.5)