##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937719_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2200278 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.490486202198085 31.0 31.0 34.0 30.0 34.0 2 31.63054804892836 33.0 31.0 34.0 30.0 34.0 3 31.64676054571286 33.0 31.0 34.0 30.0 34.0 4 35.26125153276086 37.0 35.0 37.0 33.0 37.0 5 35.249411665253206 37.0 35.0 37.0 33.0 37.0 6 35.31355901390643 37.0 35.0 37.0 33.0 37.0 7 35.245759853982086 37.0 35.0 37.0 32.0 37.0 8 35.239990583008144 37.0 35.0 37.0 32.0 37.0 9 36.828493944856056 39.0 37.0 39.0 33.0 39.0 10-11 36.695530064837264 39.0 37.0 39.0 32.0 39.0 12-13 36.567213324861676 39.0 37.0 39.0 32.0 39.0 14-15 37.64969790181058 40.0 37.0 41.0 32.0 41.0 16-17 37.56426597002742 40.0 37.0 41.0 32.0 41.0 18-19 37.51633225437877 40.0 37.0 41.0 32.0 41.0 20-21 37.4106906036419 40.0 37.0 41.0 32.0 41.0 22-23 37.328061272257415 40.0 37.0 41.0 32.0 41.0 24-25 37.352815189716935 40.0 37.0 41.0 32.0 41.0 26-27 37.25559997418508 40.0 37.0 41.0 31.0 41.0 28-29 37.13467570916039 39.5 37.0 41.0 31.0 41.0 30-31 36.89493577629736 39.0 36.0 41.0 30.5 41.0 32-33 36.72840727398992 39.0 36.0 41.0 30.0 41.0 34-35 36.69434407833919 39.0 36.0 41.0 30.0 41.0 36-37 36.53352508183057 39.0 35.5 41.0 30.0 41.0 38-39 36.371872781530335 39.0 35.0 41.0 30.0 41.0 40-41 36.146723050450895 39.0 35.0 41.0 29.5 41.0 42-43 35.848853644857606 39.0 35.0 40.0 28.5 41.0 44-45 35.58285930232452 39.0 35.0 40.0 27.0 41.0 46-47 35.19392822179743 38.0 35.0 40.0 25.5 41.0 48-49 34.83284339524369 38.0 34.0 40.0 24.0 41.0 50-51 33.92383098862962 37.5 33.5 39.5 23.5 40.5 52-53 34.02632485531374 38.0 33.5 39.5 23.0 40.5 54-55 34.31745806666248 38.0 34.0 40.0 23.0 41.0 56-57 34.05781996638606 38.0 33.5 40.0 22.0 41.0 58-59 33.78567912781931 38.0 33.0 40.0 21.5 41.0 60-61 33.4354974689562 37.5 33.0 40.0 19.5 41.0 62-63 33.05109422536607 37.0 32.0 40.0 18.0 41.0 64-65 32.64514574976435 36.0 32.0 39.0 15.5 41.0 66-67 32.17628431498201 36.0 31.0 39.0 12.0 40.5 68-69 31.645529792144448 35.0 31.0 39.0 8.0 40.0 70-71 31.15646000187249 35.0 30.0 38.0 7.0 40.0 72-73 30.682608970321024 35.0 30.0 37.5 4.0 39.5 74-75 30.138567490108066 34.0 29.0 37.0 2.0 39.0 76-77 29.63900857073515 34.0 29.0 36.0 2.0 39.0 78-79 29.061747879131637 34.0 28.5 36.0 2.0 38.0 80-81 28.470498955132037 34.0 27.0 35.0 2.0 37.0 82-83 27.9438225533319 33.5 26.0 35.0 2.0 37.0 84-85 27.40179150089216 33.0 26.0 35.0 2.0 36.0 86-87 26.85269543212267 32.5 25.0 35.0 2.0 36.0 88-89 26.332095989688575 32.0 24.5 35.0 2.0 35.0 90-91 25.731742079864453 32.0 22.0 34.5 2.0 35.0 92-93 25.04667660177487 31.5 19.0 34.0 2.0 35.0 94-95 24.25930927819121 31.0 12.0 34.0 2.0 35.0 96-97 23.37726005532028 31.0 2.0 34.0 2.0 35.0 98-99 22.289412746934705 30.5 2.0 34.0 2.0 35.0 100-101 19.48925135823746 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2836217020359939 1101 2 0.4026859494538684 1101 3 0.3171529914613309 1101 4 0.25128070757050835 1101 5 0.2075762692889569 1101 6 0.3514837035406302 1101 7 0.34891645121240344 1101 8 0.3753913975563563 1101 9 0.38203337177945684 1101 10-11 0.42423592609576133 1101 12-13 0.32448890793824603 1101 14-15 0.4646538447578621 1101 16-17 0.3515417911277723 1101 18-19 0.5166387251167777 1101 20-21 0.6674052999481717 1101 22-23 0.6321862988331262 1101 24-25 0.4300751181855773 1101 26-27 0.42354140403899265 1101 28-29 0.4482345280270934 1101 30-31 0.6206931127983211 1101 32-33 0.5586778811423017 1101 34-35 0.7387522541301763 1101 36-37 0.8198082266296396 1101 38-39 0.8016443724187496 1101 40-41 0.8078484689324767 1101 42-43 0.7884517225500431 1101 44-45 0.9749453689811389 1101 46-47 0.7794812331499017 1101 48-49 0.9647664932246798 1101 50-51 0.983227272903882 1101 52-53 0.8578739198692702 1101 54-55 0.9467289484267454 1101 56-57 0.8241151627603429 1101 58-59 0.8306683507931965 1101 60-61 0.898315996700255 1101 62-63 1.018191709710507 1101 64-65 1.079663326847779 1101 66-67 1.119710290650815 1101 68-69 1.0355129282113573 1101 70-71 1.005556751125745 1101 72-73 1.0068631244012565 1101 74-75 1.0038661709342414 1101 76-77 1.0885636750449201 1101 78-79 1.1333034810723532 1101 80-81 1.1665190351140247 1101 82-83 1.0585478679276754 1101 84-85 1.257970725583288 1101 86-87 1.366879548546141 1101 88-89 1.2221123421903926 1101 90-91 1.324733317875559 1101 92-93 1.4094358722717786 1101 94-95 1.4504523260131243 1101 96-97 1.6460001930740589 1101 98-99 1.893124680522483 1101 100-101 2.1990270006818236 1102 1 0.2448327862014601 1102 2 0.3165603485986175 1102 3 0.33040692167099195 1102 4 0.23819195707299912 1102 5 0.22234086347835813 1102 6 0.31057037434690926 1102 7 0.16730259373575507 1102 8 0.16945140026665229 1102 9 0.3966482929235582 1102 10-11 0.3278156963229151 1102 12-13 0.46683280580639064 1102 14-15 0.49247527717385964 1102 16-17 0.6020716584289048 1102 18-19 0.6206354224214721 1102 20-21 0.6084326593268656 1102 22-23 0.48745235166052225 1102 24-25 0.6564779602654554 1102 26-27 0.4576861855174812 1102 28-29 0.6255387681565949 1102 30-31 0.6060499874992757 1102 32-33 0.6094824411810791 1102 34-35 0.6824184436859184 1102 36-37 0.7838932130523162 1102 38-39 0.8577250196715198 1102 40-41 0.7193255149079434 1102 42-43 0.5744078063211049 1102 44-45 0.7051385798028207 1102 46-47 0.7140624261699244 1102 48-49 0.8178170029333174 1102 50-51 0.8246280058523325 1102 52-53 0.6734234660295826 1102 54-55 0.6877328904911408 1102 56-57 0.5711815373623068 1102 58-59 0.7546502875489196 1102 60-61 0.7640398355486795 1102 62-63 0.8854871155345876 1102 64-65 0.676098501451726 1102 66-67 0.7970844231483127 1102 68-69 0.6655911649639954 1102 70-71 0.7305289346374835 1102 72-73 0.555951725675186 1102 74-75 0.6806345321200205 1102 76-77 0.7919914799368897 1102 78-79 0.856955575292659 1102 80-81 0.9495959353717716 1102 82-83 0.8490812275998714 1102 84-85 0.9164872904116059 1102 86-87 0.9631134735824212 1102 88-89 1.1749493767853139 1102 90-91 1.176144840317864 1102 92-93 1.0049564906327078 1102 94-95 1.1376648767946484 1102 96-97 1.2648375571294714 1102 98-99 1.3109421553674103 1102 100-101 1.2673994905963113 1103 1 0.32332005467019087 1103 2 0.3643981371082248 1103 3 0.294244837147172 1103 4 0.2124536610729919 1103 5 0.23242238520614222 1103 6 0.16876667218542707 1103 7 0.28278140598017387 1103 8 0.31915835390005043 1103 9 0.2811515215916103 1103 10-11 0.30719312993588943 1103 12-13 0.34821783569574194 1103 14-15 0.37528502653599105 1103 16-17 0.3374955618073443 1103 18-19 0.2731486923077142 1103 20-21 0.28100716970158146 1103 22-23 0.2813464498449392 1103 24-25 0.41859063390999296 1103 26-27 0.39180577884020096 1103 28-29 0.405610529329131 1103 30-31 0.32290701353285556 1103 32-33 0.20878491857455828 1103 34-35 0.40453602057900184 1103 36-37 0.3767778225737217 1103 38-39 0.4084894085550914 1103 40-41 0.536527063064824 1103 42-43 0.4195499357927659 1103 44-45 0.3347127698375303 1103 46-47 0.3257926965460811 1103 48-49 0.5364138677778172 1103 50-51 0.43550417543790587 1103 52-53 0.41294799292247575 1103 54-55 0.6823310684372998 1103 56-57 0.6085981476805955 1103 58-59 0.6905310199956887 1103 60-61 0.6599869706774513 1103 62-63 0.6935590780159657 1103 64-65 0.7892999552189721 1103 66-67 0.8426394236891852 1103 68-69 0.7653150544345841 1103 70-71 0.722017371282206 1103 72-73 0.4084094685687134 1103 74-75 0.5479578053015466 1103 76-77 0.6628077956426139 1103 78-79 0.8433303705903832 1103 80-81 0.8258213820623119 1103 82-83 0.8459324357243965 1103 84-85 0.872599874678361 1103 86-87 0.9608773583585837 1103 88-89 1.0812955544858909 1103 90-91 1.1639155370176937 1103 92-93 1.097659107772941 1103 94-95 1.280601880909785 1103 96-97 1.5615610469059469 1103 98-99 1.727741566142278 1103 100-101 1.6821002984628919 1104 1 0.2688156307520977 1104 2 0.24747578490535105 1104 3 0.27459805419831795 1104 4 0.23333794840934274 1104 5 0.3015115107363897 1104 6 0.22722434629352506 1104 7 0.36631715452824665 1104 8 0.2982792695252243 1104 9 0.2905738848007502 1104 10-11 0.30647962380820815 1104 12-13 0.4107210125039842 1104 14-15 0.5060035315090232 1104 16-17 0.5339590888469345 1104 18-19 0.38934152927051713 1104 20-21 0.35656437686390063 1104 22-23 0.4630409703660163 1104 24-25 0.37161029269977774 1104 26-27 0.3255494657467892 1104 28-29 0.443128284435339 1104 30-31 0.42646235723449166 1104 32-33 0.4879328095541027 1104 34-35 0.3934759251727016 1104 36-37 0.5912024730847989 1104 38-39 0.6753728185920806 1104 40-41 0.606206924842148 1104 42-43 0.5653605311836571 1104 44-45 0.5369875046165618 1104 46-47 0.5384650496494672 1104 48-49 0.6954580957523788 1104 50-51 0.5421970049964884 1104 52-53 0.6089365496089485 1104 54-55 0.704307877637099 1104 56-57 0.7104840411311457 1104 58-59 0.6969843932557467 1104 60-61 0.7685333552795655 1104 62-63 0.7227589986107716 1104 64-65 0.843950762458455 1104 66-67 1.0345745731762648 1104 68-69 0.75055985577551 1104 70-71 0.7490609644954631 1104 72-73 0.8873788979679276 1104 74-75 0.8524917460206041 1104 76-77 0.7933360923490973 1104 78-79 0.7622927439733154 1104 80-81 0.7805407083028051 1104 82-83 0.8357393338107855 1104 84-85 0.7642369945269785 1104 86-87 0.9977737597902809 1104 88-89 1.0066609724818143 1104 90-91 1.1998291790627462 1104 92-93 1.2579506457163419 1104 94-95 1.3302083168931453 1104 96-97 1.4412048401530626 1104 98-99 1.5301439936039856 1104 100-101 1.6698745395609222 1105 1 0.23230067023763823 1105 2 0.26075076304777767 1105 3 0.2896649986867814 1105 4 0.2202407478344668 1105 5 0.13968405983020205 1105 6 0.16530276393915244 1105 7 0.11836888898296394 1105 8 0.1373342023994084 1105 9 0.2457768941689551 1105 10-11 0.2711850609636386 1105 12-13 0.18758434609748065 1105 14-15 0.32290998108996405 1105 16-17 0.35150214767751464 1105 18-19 0.30892772931640167 1105 20-21 0.3180514881873435 1105 22-23 0.41765910730441647 1105 24-25 0.3031086737807769 1105 26-27 0.3717744911520313 1105 28-29 0.32527476526728805 1105 30-31 0.48811313429196446 1105 32-33 0.3332874147026317 1105 34-35 0.47390007249077826 1105 36-37 0.5402053419653683 1105 38-39 0.46160160058317956 1105 40-41 0.5406138021141302 1105 42-43 0.5098237651271162 1105 44-45 0.4864274264417361 1105 46-47 0.3922622168762686 1105 48-49 0.5158620528997133 1105 50-51 0.4582634260068872 1105 52-53 0.4671298683258911 1105 54-55 0.4216924936887878 1105 56-57 0.39822848528651633 1105 58-59 0.5007167761295008 1105 60-61 0.6069975029445303 1105 62-63 0.4600177473562752 1105 64-65 0.656793752937304 1105 66-67 0.8579479281424867 1105 68-69 0.6899118861057012 1105 70-71 0.7139431937552061 1105 72-73 0.7875076191756492 1105 74-75 0.6486798165545444 1105 76-77 0.5921560395440366 1105 78-79 0.6914000956078823 1105 80-81 0.6014799690792394 1105 82-83 0.6597955162523554 1105 84-85 0.8091602229991359 1105 86-87 0.6977504634394087 1105 88-89 0.8864762414856848 1105 90-91 1.0115183891863708 1105 92-93 0.9983080850571362 1105 94-95 1.1455086584941263 1105 96-97 1.2623790353646562 1105 98-99 1.2487404337954366 1105 100-101 1.52716477976956 1106 1 0.11719630124432001 1106 2 0.26465050105639776 1106 3 0.100372766319893 1106 4 0.2821080125939801 1106 5 0.24678881484749127 1106 6 0.19457278771138675 1106 7 0.2703411946129677 1106 8 0.18627699348306948 1106 9 0.2774774845250363 1106 10-11 0.07663019791664283 1106 12-13 0.05869366825077549 1106 14-15 0.08485697527436287 1106 16-17 0.20622031352612424 1106 18-19 0.22399756789484826 1106 20-21 0.16576482547864657 1106 22-23 0.1781272591046772 1106 24-25 0.12304243483519173 1106 26-27 0.3175762971578777 1106 28-29 0.292398797051554 1106 30-31 0.33948555540339953 1106 32-33 0.40250277617662533 1106 34-35 0.6412030882827437 1106 36-37 0.430551932860574 1106 38-39 0.415757792073876 1106 40-41 0.3936753835256894 1106 42-43 0.3996322434841133 1106 44-45 0.4278571444243511 1106 46-47 0.5217699019295665 1106 48-49 0.5153018498543602 1106 50-51 0.7541061147208907 1106 52-53 0.6600128770000282 1106 54-55 0.5899617910192845 1106 56-57 0.45515857259798054 1106 58-59 0.398214786965859 1106 60-61 0.5096753814088686 1106 62-63 0.6790095976897277 1106 64-65 0.6943009908868802 1106 66-67 0.7803559888769271 1106 68-69 0.7316674237988217 1106 70-71 0.5337304652253465 1106 72-73 0.9728770050310445 1106 74-75 0.734022469842305 1106 76-77 0.8471469671661715 1106 78-79 0.9705377799363681 1106 80-81 0.854302973013592 1106 82-83 0.7093136785176135 1106 84-85 0.827754627443138 1106 86-87 1.0633503927497863 1106 88-89 1.2193005769584389 1106 90-91 1.247988411655573 1106 92-93 1.233198992563672 1106 94-95 1.677552924205667 1106 96-97 1.8682881544074483 1106 98-99 1.9394063495884595 1106 100-101 1.9841655567038927 1107 1 0.2476436767899628 1107 2 0.2848864831975888 1107 3 0.24580883255888608 1107 4 0.26007056470615453 1107 5 0.2673936175379552 1107 6 0.21104558205003343 1107 7 0.2991780887658777 1107 8 0.2536707575173125 1107 9 0.35754703954388134 1107 10-11 0.3299176051102819 1107 12-13 0.3815116252747828 1107 14-15 0.49667357820606384 1107 16-17 0.4446268827325852 1107 18-19 0.5361892504033676 1107 20-21 0.5033019989564167 1107 22-23 0.5042700619686258 1107 24-25 0.46272563562626345 1107 26-27 0.4246850612036752 1107 28-29 0.38475505417829226 1107 30-31 0.4411799455714345 1107 32-33 0.48779536366071596 1107 34-35 0.557499096598427 1107 36-37 0.4946895323302769 1107 38-39 0.527001399936978 1107 40-41 0.6057030921146094 1107 42-43 0.6291187532844233 1107 44-45 0.523340814978539 1107 46-47 0.6246242075209167 1107 48-49 0.6542211762401635 1107 50-51 0.449989312865533 1107 52-53 0.3810825700115288 1107 54-55 0.5625354884431957 1107 56-57 0.545589023565114 1107 58-59 0.5480484501583476 1107 60-61 0.5431500469447101 1107 62-63 0.7477105113187221 1107 64-65 0.7622141701935305 1107 66-67 0.8887091022075495 1107 68-69 0.9880559901097534 1107 70-71 0.8038180054968294 1107 72-73 0.8277323616349399 1107 74-75 0.8156559416093607 1107 76-77 0.8934169333065114 1107 78-79 0.9927017735992081 1107 80-81 1.0752567269913804 1107 82-83 1.0531999843324407 1107 84-85 1.1204888206156305 1107 86-87 0.998839982075669 1107 88-89 1.1530553864855193 1107 90-91 1.2727504268356036 1107 92-93 1.2187599621080452 1107 94-95 1.3782824579732242 1107 96-97 1.5217820885066082 1107 98-99 1.6375845945109262 1107 100-101 1.5950845562760527 1108 1 0.06712790076467456 1108 2 0.12712268892721568 1108 3 0.1455671243906167 1108 4 0.13660108278406824 1108 5 0.07844982369670817 1108 6 0.2749006596401671 1108 7 0.260590607719962 1108 8 0.2181484268531193 1108 9 0.2247837816852396 1108 10-11 0.3519088196235245 1108 12-13 0.32013268673673423 1108 14-15 0.3010738001034525 1108 16-17 0.34627209397152825 1108 18-19 0.28431134063011854 1108 20-21 0.32724406482277857 1108 22-23 0.32125033680724613 1108 24-25 0.27959352439508933 1108 26-27 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2316 56-57 -1.0498718012422188 2316 58-59 -1.0116931003302412 2316 60-61 -1.2193800616861097 2316 62-63 -1.0498099532634484 2316 64-65 -1.0338630511832996 2316 66-67 -1.0698252791054585 2316 68-69 -1.0680196468267305 2316 70-71 -1.097039146403425 2316 72-73 -1.056866177003137 2316 74-75 -1.1498035432466303 2316 76-77 -1.3713612422028554 2316 78-79 -1.2682657849848091 2316 80-81 -1.2849765875346186 2316 82-83 -1.2519817041616612 2316 84-85 -1.4336968594930681 2316 86-87 -1.4339581414617832 2316 88-89 -1.5971510550983865 2316 90-91 -1.6251822386194839 2316 92-93 -1.6411938958051842 2316 94-95 -1.5035709809161837 2316 96-97 -1.7319946505825001 2316 98-99 -1.8013769261424244 2316 100-101 -1.8697571674206728 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9384.0 3 1859.0 4 1823.0 5 2098.0 6 2413.0 7 2919.0 8 3742.0 9 4525.0 10 5843.0 11 7698.0 12 9709.0 13 12205.0 14 14017.0 15 17111.0 16 21170.0 17 27118.0 18 23807.0 19 17843.0 20 17823.0 21 19106.0 22 20915.0 23 22838.0 24 25860.0 25 29267.0 26 33727.0 27 39142.0 28 46046.0 29 54557.0 30 66175.0 31 79709.0 32 98957.0 33 125481.0 34 163525.0 35 220850.0 36 300357.0 37 375374.0 38 251473.0 39 23812.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2329720949137 19.442828854004297 15.043009678489138 22.281189372592866 2 14.907940213575058 20.490620753805047 40.73474561587496 23.86669341674494 3 19.43884878965151 24.767163512176438 30.187536761856126 25.606450936315927 4 12.25255729928039 16.580883794606258 38.14149067042871 33.02506823568464 5 13.099350402094034 38.03000143645728 34.36794739374206 14.502700767706617 6 31.016517753031714 36.63361962161847 17.560679876982277 14.789182748367539 7 27.57546717776713 33.569662640900304 20.911909867530483 17.942960313802082 8 25.827922558546824 34.72344127553844 19.91327577529134 19.5353603906234 9 25.89718865477839 17.19323688440476 21.245394550755478 35.664179910061364 10-11 24.60854396872679 26.95501401477615 27.242438204368618 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21.662175097428648 26.34922398616372 30.36957311725227 21.61902779915536 38-39 21.40120581717313 26.939213281929085 30.16885629502406 21.490724605873726 40-41 21.62530392795937 26.917606408084378 29.97212935929857 21.484960304657683 42-43 21.328096143975493 26.901016392442163 29.621415789993836 22.149471673588508 44-45 21.56466002543296 26.8246692439706 29.576407284466583 22.034263446129852 46-47 21.9586332860393 27.254547400688228 29.149868935565493 21.636950377706977 48-49 21.90303583234588 27.22526777411846 28.99646793918633 21.875228454349326 50-51 21.50764726165765 27.543852696663556 29.150536062178382 21.79796397950041 52-53 21.906546067424944 27.805192219280293 28.394314138515988 21.893947574778775 54-55 21.639741606868622 27.411232012766636 28.725764258167235 22.223262122197507 56-57 21.97116748421479 27.56619271186927 28.484414677891507 21.97822512602444 58-59 22.277881188154876 27.29417742913708 28.47785186493836 21.950089517769687 60-61 21.90336827626963 27.372955765379608 29.114859129102953 21.608816829247804 62-63 21.592135896115437 27.583670391725366 29.0993745125118 21.724819199647392 64-65 21.580395986859628 27.836482970106342 28.79104943119859 21.79207161183544 66-67 21.62646146659227 27.49099563332894 28.51219246226202 22.370350437816768 68-69 21.56507563545623 27.43258380918554 28.82220886496726 22.180131690390972 70-71 21.945869376552107 27.416599353612547 28.563235871316262 22.074295398519084 72-73 21.81463371102062 27.28254334381078 28.8141608715181 22.0886620736505 74-75 21.52005134440984 28.150838547193622 28.295660199817906 22.03344990857863 76-77 21.7369278229443 27.57754463266211 28.28186763308629 22.403659911307304 78-79 21.839234085455903 27.533524016570404 28.426389697822778 22.200852200150916 80-81 21.89443316374723 27.615920831588863 28.223962932835573 22.26568307182833 82-83 21.867873614676796 27.523017861765116 28.544971539421027 22.06413698413707 84-85 21.830256817445086 27.634236376947257 28.33023132145706 22.2052754841506 86-87 21.715483990807144 27.809894704637582 28.22196160416082 22.252659700394446 88-89 21.748289112401007 27.643791164373376 28.012638519802497 22.595281203423117 90-91 21.829441741322835 27.30304101750198 28.26127492374632 22.606242317428872 92-93 22.054895308183987 27.439732034115842 28.25488447226574 22.25048818543443 94-95 21.834104380890285 27.31987572478508 28.264461154205943 22.581558740118687 96-97 21.47141021566526 27.33274627846981 28.585071807980245 22.61077169788469 98-99 21.596649886196218 27.444231365128573 28.103831124611784 22.85528762406343 100-101 21.79742653811724 26.607400825348687 28.767141371519124 22.828031265014953 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3062.0 1 1755.0 2 472.0 3 512.5 4 466.0 5 441.5 6 767.5 7 1586.0 8 2614.5 9 3220.5 10 3067.5 11 2815.5 12 2929.0 13 3158.0 14 3367.5 15 3549.0 16 3818.0 17 4060.5 18 4236.5 19 4479.5 20 4963.5 21 5728.5 22 6742.0 23 8214.0 24 9923.5 25 12225.5 26 15416.0 27 19033.0 28 21710.0 29 24348.0 30 28095.0 31 32513.0 32 36830.5 33 40665.0 34 45015.5 35 49091.5 36 53930.0 37 58868.0 38 61954.5 39 64195.0 40 66278.5 41 69282.5 42 71899.0 43 74866.0 44 78832.5 45 81531.5 46 82963.0 47 88957.5 48 100021.0 49 98208.5 50 85027.0 51 76161.0 52 70809.5 53 67122.0 54 64839.0 55 60516.5 56 53911.0 57 47820.5 58 43230.5 59 38449.0 60 31795.0 61 25352.0 62 19694.0 63 14639.5 64 10987.5 65 8359.5 66 6062.0 67 4284.5 68 2983.5 69 2053.0 70 1490.5 71 1026.0 72 706.0 73 543.5 74 409.5 75 262.0 76 170.0 77 111.5 78 78.0 79 50.0 80 34.0 81 31.0 82 25.5 83 19.5 84 15.0 85 11.5 86 10.0 87 7.0 88 7.5 89 7.0 90 4.5 91 2.5 92 2.0 93 2.0 94 2.5 95 2.0 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3679989528595932 2 0.32441355137850764 3 0.3230500873071494 4 0.32468624419277925 5 0.33554850796126673 6 0.2191541250696503 7 0.2160636065079049 8 0.21874508584824282 9 0.21729072417212736 10-11 0.2234490368944288 12-13 0.20990529378560346 14-15 0.21254132432356274 16-17 0.2208584551588481 18-19 0.21249587552118415 20-21 0.20470140591325275 22-23 0.20240624139313307 24-25 0.2062921139965041 26-27 0.2020426509741042 28-29 0.2234263124932395 30-31 0.22928920800007999 32-33 0.23119805769998156 34-35 0.22753942910850355 36-37 0.19597523585655996 38-39 0.18593105053088746 40-41 0.19268019768411082 42-43 0.20774647567261953 44-45 0.21370026878421727 46-47 0.2178361098006706 48-49 0.21894960545894654 50-51 0.22347176129561808 52-53 0.2531952780512281 54-55 0.20697384603218322 56-57 0.18529476729758695 58-59 0.18479483047142226 60-61 0.17668221924684063 62-63 0.18502207448331529 64-65 0.1814770678977838 66-67 0.18099985547280845 68-69 0.19170304843297073 70-71 0.1850675232856939 72-73 0.18054536744902236 74-75 0.18763538062008528 76-77 0.19399821295309047 78-79 0.19611158226369574 80-81 0.1839994764297966 82-83 0.18154524110135176 84-85 0.17515968436715726 86-87 0.1722509610149263 88-89 0.18072716265853678 90-91 0.1888170494819291 92-93 0.17629590442662246 94-95 0.18286325637033138 96-97 0.1819770047239485 98-99 0.18968057672712266 100-101 0.1595025719477266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2200278.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.25816703509497 #Duplication Level Percentage of deduplicated Percentage of total 1 73.03182136988268 37.43477298654665 2 14.235035133180068 14.593236172139786 3 5.300845834198531 8.151349235899069 4 2.477061535912551 5.0787853585605784 5 1.3771658317899411 3.5295498120457154 6 0.8311147311979896 2.5560850630244767 7 0.5137640527451512 1.8434222542566812 8 0.3797669678492358 1.5572926937942138 9 0.286532183374708 1.321840306471612 >10 1.4287460035813446 13.116468048484153 >50 0.09127174696113113 3.1711807690770124 >100 0.04314138077881244 3.7971609586766575 >500 0.0018666142739367495 0.6560405612765505 >1k 0.0014221823039517595 1.4873107911876622 >5k 3.555455759879399E-4 1.2055582506685927 >10k+ 8.888639399698497E-5 0.49994673789064503 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10973 0.49870970850047125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8293 0.37690691812580046 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7547 0.3430021115513585 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5581 0.25364976607501416 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5039 0.22901651518580832 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3980 0.18088623346686192 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3278 0.14898117419707874 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3091 0.14048224815227894 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2333 0.10603205594929369 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2319 0.10539577271599315 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 6.817320356791278E-5 0.0 0.0 0.0 0.0 14-15 1.817952095144341E-4 0.0 0.0 4.5448802378608525E-5 0.0 16-17 2.0451961070373835E-4 0.0 0.0 4.5448802378608525E-5 0.0 18-19 3.181416166502597E-4 0.0 0.0 4.5448802378608525E-5 0.0 20-21 3.635904190288682E-4 0.0 0.0 6.817320356791278E-5 0.0 22-23 4.090392214074767E-4 0.0 0.0 9.089760475721705E-5 0.0 24-25 4.999368261646937E-4 0.0 0.0 1.3634640713582557E-4 0.0 26-27 4.999368261646937E-4 0.0 0.0 2.7269281427165113E-4 0.0 28-29 6.362832333005194E-4 0.0 0.0 7.499052392470406E-4 0.0 30-31 7.271808380577364E-4 0.0 0.0 0.002249715717741122 0.0 32-33 7.271808380577364E-4 0.0 0.0 0.00463577784261807 0.0 34-35 7.72629640436345E-4 0.0 0.0 0.006885493560359191 0.0 36-37 8.180784428149534E-4 0.0 0.0 0.00890796526620727 0.0 38-39 9.998736523293874E-4 0.0 0.0 0.01179396421724891 0.0 40-41 0.001045322454707996 0.0 0.0 0.01567983682061994 0.0 42-43 0.001136220059465213 0.0 0.0 0.02117914190843157 0.0 44-45 0.0013180152689796472 0.0 0.0 0.026542100589107376 2.2724401189304262E-5 46-47 0.0015225348796833855 0.0 0.0 0.03354121615541309 4.5448802378608525E-5 48-49 0.0015452592808726897 0.0 0.0 0.040199465703879236 4.5448802378608525E-5 50-51 0.0015452592808726897 0.0 0.0 0.050470895041444766 4.5448802378608525E-5 52-53 0.0015452592808726897 0.0 0.0 0.07226359578198754 4.5448802378608525E-5 54-55 0.0015452592808726897 0.0 0.0 0.10535032391361455 4.5448802378608525E-5 56-57 0.0015907080832512981 0.0 0.0 0.13302864456218713 4.5448802378608525E-5 58-59 0.0015907080832512981 0.0 0.0 0.1691831668543702 4.5448802378608525E-5 60-61 0.0015907080832512981 0.0 0.0 0.21463196923297873 4.5448802378608525E-5 62-63 0.0015907080832512981 0.0 0.0 0.24769597296341644 4.5448802378608525E-5 64-65 0.0015907080832512981 0.0 0.0 0.2752379472048532 4.5448802378608525E-5 66-67 0.0015907080832512981 0.0 0.0 0.3456835908916964 4.5448802378608525E-5 68-69 0.0016361568856299068 0.0 0.0 0.4225375157139234 4.5448802378608525E-5 70-71 0.001658881286819211 0.0 0.0 0.46148713935239094 4.5448802378608525E-5 72-73 0.0017270544903871237 0.0 0.0 0.537454812528235 4.5448802378608525E-5 74-75 0.0017952276939550366 0.0 0.0 0.6479635755118216 4.5448802378608525E-5 76-77 0.0018179520951443408 0.0 0.0 0.7504279004743946 4.5448802378608525E-5 78-79 0.0018179520951443408 0.0 0.0 0.8708445023765179 9.089760475721705E-5 80-81 0.0019542985022801664 0.0 0.0 1.019030322531971 9.089760475721705E-5 82-83 0.0020451961070373833 0.0 0.0 1.1818733814545253 9.089760475721705E-5 84-85 0.0020679205082266877 0.0 0.0 1.335422160290654 9.089760475721705E-5 86-87 0.002090644909415992 0.0 0.0 1.5529628528758639 9.089760475721705E-5 88-89 0.0021360937117946006 0.0 0.0 1.8177248511324477 9.089760475721705E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9835 0.0 26.460007 1 TACATGG 9790 0.0 26.135923 2 ACATGGG 10030 0.0 24.798145 3 GAGTACT 9245 0.0 23.889673 12-13 GTATCAA 19635 0.0 23.841917 1 CATGGGA 5795 0.0 22.569786 4 GTACTTT 9880 0.0 22.184984 14-15 AGTACTT 9630 0.0 22.096111 12-13 AGAGTAC 14730 0.0 21.362265 10-11 TCAACGC 22320 0.0 20.77584 4 ATCAACG 22335 0.0 20.760466 3 CAACGCA 22365 0.0 20.691507 5 TATCAAC 22440 0.0 20.643076 2 AACGCAG 22720 0.0 20.385427 6 GGTATCA 14880 0.0 19.599028 1 ACTTTTT 11905 0.0 18.809483 16-17 CGCAGAG 24580 0.0 18.668474 8 ACGCAGA 24695 0.0 18.658907 7 GTGTAGC 1600 0.0 18.435234 1 TACTTTT 11350 0.0 18.432932 14-15 >>END_MODULE