FastQCFastQC Report
Thu 26 May 2016
SRR937719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200278
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121650.5528846809357726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101060.4593055968382177No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96400.4381264549297862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69110.3140966732385635No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36280.1648882550295917No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35670.1621158780844966No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26920.12234817600321414No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26240.11925765744146875No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25450.11566720205355868No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24280.11034969217526149No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23900.10862263768487436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG99500.024.0248741
TACATGG98550.023.4822222
GAGTACT106450.022.66871612-13
ACATGGG98550.022.6142963
GTACTTT110450.021.73826414-15
AGTACTT111300.021.06206312-13
AGAGTAC161400.020.79301610-11
GTATCAA226550.020.4110341
GGACCGA6400.020.0379456
CATGGGG41150.019.7466354
GGTATCA179900.018.5448021
TACTTTT124750.018.54205314-15
ACTTTTT132950.018.32729716-17
TATCGTT1300.007563734418.2698757
GTACCGT2101.9580608E-518.0942296
CATGGGA59100.018.090974
TGTACGC1851.5174963E-417.9801432
TCAACGC257350.017.8738464
CAACGCA258350.017.8046635
TATCAAC260450.017.7340832
Produced by FastQC (version 0.11.5)