##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937719_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2200278 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04430758295088 31.0 31.0 34.0 30.0 34.0 2 32.31099070208401 33.0 31.0 34.0 31.0 34.0 3 32.40238915264344 34.0 31.0 34.0 31.0 34.0 4 36.06685609727498 37.0 35.0 37.0 35.0 37.0 5 35.968387631017535 37.0 35.0 37.0 35.0 37.0 6 36.01198348572317 37.0 35.0 37.0 35.0 37.0 7 35.950844847787415 37.0 35.0 37.0 35.0 37.0 8 35.92708057799969 37.0 35.0 37.0 35.0 37.0 9 37.59515343061195 39.0 37.0 39.0 35.0 39.0 10-11 37.506226940413896 39.0 37.0 39.0 35.0 39.0 12-13 37.39959541476122 39.0 37.0 39.0 34.0 39.0 14-15 38.561556539673624 40.0 38.0 41.0 34.0 41.0 16-17 38.53196686964102 40.0 38.0 41.0 34.0 41.0 18-19 38.52361701566802 40.0 38.0 41.0 34.0 41.0 20-21 38.424903807609766 40.0 38.0 41.0 34.0 41.0 22-23 38.372502701931296 40.0 38.0 41.0 34.0 41.0 24-25 38.393291211383286 40.0 38.0 41.0 34.0 41.0 26-27 38.25121916412381 40.0 38.0 41.0 34.0 41.0 28-29 38.10349169514034 40.0 37.5 41.0 33.0 41.0 30-31 38.143452100143705 40.0 37.5 41.0 34.0 41.0 32-33 38.050894705123625 40.0 37.0 41.0 33.5 41.0 34-35 37.89970085598274 40.0 37.0 41.0 33.0 41.0 36-37 37.74573599336084 40.0 37.0 41.0 33.0 41.0 38-39 37.608631272957325 40.0 37.0 41.0 33.0 41.0 40-41 37.44486219468631 40.0 37.0 41.0 33.0 41.0 42-43 37.20555402544588 40.0 36.0 41.0 32.0 41.0 44-45 36.885258135562864 39.5 36.0 41.0 31.0 41.0 46-47 36.497184219448634 39.0 36.0 41.0 30.5 41.0 48-49 36.14731820251804 39.0 35.5 41.0 30.0 41.0 50-51 35.875919997382155 39.0 35.0 40.0 29.0 41.0 52-53 35.64375410743551 39.0 35.0 40.0 28.0 41.0 54-55 35.41648941633739 38.5 35.0 40.0 28.0 41.0 56-57 35.13026558462158 38.0 34.5 40.0 27.0 41.0 58-59 34.86756014467262 38.0 34.0 40.0 26.5 41.0 60-61 34.5122879926991 38.0 34.0 40.0 26.0 41.0 62-63 34.14360208119156 37.0 33.5 40.0 26.0 41.0 64-65 33.725706706152586 37.0 33.0 39.5 24.5 41.0 66-67 33.281241052267035 36.0 32.5 39.0 23.0 40.0 68-69 32.81529084052106 35.5 32.0 39.0 22.0 40.0 70-71 32.405405589657306 35.0 31.5 38.0 21.5 40.0 72-73 31.899717444795613 35.0 31.0 37.5 20.5 39.5 74-75 31.41064833625569 35.0 31.0 37.0 18.5 39.0 76-77 30.19324194488151 33.5 29.5 35.5 18.0 38.0 78-79 30.799454205332236 34.0 30.5 36.0 18.0 38.0 80-81 30.610711010154173 34.0 30.5 35.5 18.0 37.0 82-83 30.23256538491954 34.0 30.0 35.0 13.5 37.0 84-85 29.838839001253476 34.0 30.0 35.0 8.5 36.5 86-87 29.46838967621364 34.0 29.5 35.0 6.5 36.0 88-89 29.009068626782614 34.0 29.0 35.0 2.0 36.0 90-91 28.44243886454348 33.5 29.0 35.0 2.0 35.0 92-93 27.9801800045267 33.0 27.5 35.0 2.0 35.0 94-95 27.471813561740834 33.0 27.0 35.0 2.0 35.0 96-97 26.855082175979582 33.0 25.5 34.5 2.0 35.0 98-99 25.918691183568622 32.0 24.5 34.0 2.0 35.0 100-101 23.11201925393064 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3623653120189303 1101 2 0.2825506530323807 1101 3 0.3192199486331049 1101 4 0.16923311572956834 1101 5 0.11143284191306435 1101 6 0.21429803216766175 1101 7 0.27486639321831063 1101 8 0.19173204435116986 1101 9 0.2565501594697963 1101 10-11 0.3281659072789793 1101 12-13 0.31108876844790956 1101 14-15 0.3425113798811168 1101 16-17 0.3971658523762116 1101 18-19 0.35944387935015243 1101 20-21 0.3575748967908794 1101 22-23 0.3446704653194246 1101 24-25 0.2715216694822331 1101 26-27 0.25269093463568026 1101 28-29 0.17534475044086406 1101 30-31 0.27167320899223313 1101 32-33 0.3254472327424267 1101 34-35 0.32043091876229113 1101 36-37 0.2743096352044745 1101 38-39 0.26981542548427484 1101 40-41 0.28579414875835596 1101 42-43 0.3252287845310988 1101 44-45 0.22498526644397288 1101 46-47 0.11459892703526009 1101 48-49 0.08031894026632358 1101 50-51 0.18692612781638474 1101 52-53 0.18110359931207398 1101 54-55 0.10254497406857155 1101 56-57 0.11107785116988822 1101 58-59 0.020792510202589654 1101 60-61 0.14194558947777125 1101 62-63 0.033175233242431545 1101 64-65 -0.03947281201732267 1101 66-67 -0.02448028905343591 1101 68-69 0.1541791894019866 1101 70-71 -0.06729134477831167 1101 72-73 -0.16176633529019213 1101 74-75 -0.16788213825194376 1101 76-77 -0.14155545822886495 1101 78-79 -0.2160598008061676 1101 80-81 -0.19696145278261667 1101 82-83 -0.13474651717592323 1101 84-85 -0.10871817658489036 1101 86-87 0.010991539489193514 1101 88-89 -0.1532804541474455 1101 90-91 -0.311328520982876 1101 92-93 -0.3375053306091438 1101 94-95 -0.3046790143410085 1101 96-97 -0.36559144435699054 1101 98-99 -0.37523900539355637 1101 100-101 -0.2711901647688286 1102 1 0.25235494952025306 1102 2 0.2222885993518986 1102 3 0.19974602466656677 1102 4 0.0448748164378614 1102 5 0.09864613431778935 1102 6 0.10268778617124497 1102 7 0.08043548901557784 1102 8 0.13951120873959155 1102 9 0.16092796120425135 1102 10-11 0.1780746314421222 1102 12-13 0.15758164358395987 1102 14-15 0.3346866933124275 1102 16-17 0.2593218094420706 1102 18-19 0.278347382710713 1102 20-21 0.26847718478833826 1102 22-23 0.2403272277206554 1102 24-25 0.20576194056573627 1102 26-27 0.20037492404709667 1102 28-29 0.16826179943676323 1102 30-31 0.14674273616699196 1102 32-33 0.19804400838949476 1102 34-35 0.10319420230124621 1102 36-37 0.21299604972374198 1102 38-39 0.2333938607896684 1102 40-41 0.24190758474581742 1102 42-43 0.3607750091184272 1102 44-45 0.15447993563395102 1102 46-47 0.004226967044260732 1102 48-49 0.1590078583257295 1102 50-51 0.06993416948392195 1102 52-53 0.1424391862809955 1102 54-55 0.16781062462155916 1102 56-57 0.08492585751818638 1102 58-59 0.12244253192316279 1102 60-61 0.07835791759255528 1102 62-63 0.014594201132176465 1102 64-65 0.12371024823011822 1102 66-67 0.2281781491914856 1102 68-69 0.12545050460780516 1102 70-71 0.11903901149971574 1102 72-73 -0.05905759027170987 1102 74-75 -0.0898756259013318 1102 76-77 -0.010483895860261327 1102 78-79 0.12726016308352683 1102 80-81 0.1586436117397234 1102 82-83 -0.07063341649203281 1102 84-85 -0.05381614838028881 1102 86-87 -0.15644776370861635 1102 88-89 -0.13403533217402597 1102 90-91 -0.11464488013436025 1102 92-93 -0.1346785776085504 1102 94-95 -0.3173158241566867 1102 96-97 -0.2622898502491644 1102 98-99 -0.3277702889702674 1102 100-101 -0.24669558659623547 1103 1 0.2735905263891141 1103 2 0.2054145748331493 1103 3 0.19448797972624732 1103 4 0.14279882945027111 1103 5 0.06013078284800599 1103 6 0.09897192566574375 1103 7 0.12360913543288632 1103 8 0.10795583899330552 1103 9 0.20321847993370312 1103 10-11 0.26371922277055404 1103 12-13 0.2490879975508875 1103 14-15 0.21843021081272695 1103 16-17 0.27455341275840794 1103 18-19 0.20095989952648097 1103 20-21 0.17496035273796195 1103 22-23 0.19332297058160464 1103 24-25 0.16999098826214976 1103 26-27 0.09249103080092169 1103 28-29 0.12447681315040882 1103 30-31 0.10357357952156576 1103 32-33 0.13902731437350013 1103 34-35 0.14510653605960755 1103 36-37 0.2635303116950425 1103 38-39 0.3591330547440563 1103 40-41 0.2451944110033466 1103 42-43 0.25971128818761 1103 44-45 0.26994580062922324 1103 46-47 0.3079749659633677 1103 48-49 0.19847848707338045 1103 50-51 0.21891419772563125 1103 52-53 0.3177002508539317 1103 54-55 0.06866236567157102 1103 56-57 0.25756458207214195 1103 58-59 0.2724422604485781 1103 60-61 0.32044401596408534 1103 62-63 0.16842172122024834 1103 64-65 0.2510422670696215 1103 66-67 0.22467717125652342 1103 68-69 0.36926558280710253 1103 70-71 0.32449890823078675 1103 72-73 0.30340482616110265 1103 74-75 0.18284669194026648 1103 76-77 0.15576797157159916 1103 78-79 0.37214829676262084 1103 80-81 0.3292409518735653 1103 82-83 0.2809661534922334 1103 84-85 0.26265544639921146 1103 86-87 0.11045038028233378 1103 88-89 0.11286474137470037 1103 90-91 0.2700486939788256 1103 92-93 0.25934495130203317 1103 94-95 -0.022798900640264463 1103 96-97 -0.11413113702196043 1103 98-99 -0.05947540827080999 1103 100-101 0.06449217315794797 1104 1 0.2539005188368151 1104 2 0.15967453960509914 1104 3 0.1381313015049983 1104 4 0.07867813194925333 1104 5 0.03071303826779115 1104 6 0.0314326823284361 1104 7 0.07396847997179634 1104 8 0.16687177394347685 1104 9 0.13784186756593897 1104 10-11 0.15650799814149252 1104 12-13 0.15374752975662176 1104 14-15 0.21062576852417436 1104 16-17 0.1510531163897113 1104 18-19 0.21513846996877106 1104 20-21 0.048864078738802164 1104 22-23 0.10359419696366245 1104 24-25 0.15400829091081647 1104 26-27 0.116720522227439 1104 28-29 0.06463485110560896 1104 30-31 0.16437328671489837 1104 32-33 0.19425615062710477 1104 34-35 0.12243323041403187 1104 36-37 0.18393490599011386 1104 38-39 0.10062261059925248 1104 40-41 0.12798999825160706 1104 42-43 0.1832679766546832 1104 44-45 0.11004358428768768 1104 46-47 0.0799228587039238 1104 48-49 0.1309985775088549 1104 50-51 0.050040875030354925 1104 52-53 -0.005371041325574311 1104 54-55 -0.0859047139101321 1104 56-57 0.08206558804474184 1104 58-59 0.09981540124032051 1104 60-61 0.023256356748461826 1104 62-63 0.1747671330286522 1104 64-65 0.19881694041368547 1104 66-67 0.10513372712812696 1104 68-69 0.11419239514868451 1104 70-71 0.09535868161380279 1104 72-73 0.18996265487720976 1104 74-75 0.1738023438973606 1104 76-77 0.19673649085277134 1104 78-79 0.09434146911632624 1104 80-81 0.11138594880582176 1104 82-83 0.06754917654895465 1104 84-85 0.06704940744183929 1104 86-87 0.06334676890673663 1104 88-89 0.08595902664187705 1104 90-91 0.20259339842459667 1104 92-93 0.18132384168643867 1104 94-95 0.09020802290061525 1104 96-97 0.29826900113667776 1104 98-99 0.45863379949024363 1104 100-101 0.43173060527351126 1105 1 0.26291691615666934 1105 2 0.17720120032745257 1105 3 0.13526399767722808 1105 4 0.08940154868430739 1105 5 0.04981033029365278 1105 6 0.10028325377885494 1105 7 0.10106950729750963 1105 8 0.02215373862605219 1105 9 0.15637783973330244 1105 10-11 0.15813568776629694 1105 12-13 0.14289324350398402 1105 14-15 0.20453353626074033 1105 16-17 0.11450051117968485 1105 18-19 0.19326650765919595 1105 20-21 0.07372682341946302 1105 22-23 0.02166091124791336 1105 24-25 -0.013151698651149957 1105 26-27 -0.05651268015753885 1105 28-29 0.050948343516367345 1105 30-31 0.04817367049370347 1105 32-33 0.05338457540748465 1105 34-35 0.18750287507176466 1105 36-37 0.028048615833469626 1105 38-39 0.08007422396024566 1105 40-41 0.1881962832996038 1105 42-43 -0.00416476547954403 1105 44-45 -0.011845998104526245 1105 46-47 0.045100726934080626 1105 48-49 0.08760439379170037 1105 50-51 0.13682165439775673 1105 52-53 0.10015512114657099 1105 54-55 0.09897006130570674 1105 56-57 0.1516641260903242 1105 58-59 0.11601543135608239 1105 60-61 0.022611371061323382 1105 62-63 -0.1430275145657589 1105 64-65 -0.08740691426122993 1105 66-67 -0.023023499983310103 1105 68-69 -0.03369833905295394 1105 70-71 -0.15441633712971736 1105 72-73 -0.04823051010746937 1105 74-75 -0.12944479687960708 1105 76-77 -0.08972466336906848 1105 78-79 0.10140408702700299 1105 80-81 -0.03460234862117417 1105 82-83 -0.02511945000699356 1105 84-85 -0.05021963348169223 1105 86-87 -0.26722953393478477 1105 88-89 -0.09850333221022467 1105 90-91 0.024156654521263476 1105 92-93 0.29418924159924487 1105 94-95 0.057606533983925345 1105 96-97 0.08410123272211578 1105 98-99 0.22368497656695396 1105 100-101 0.02789230481128513 1106 1 0.1259567898274767 1106 2 0.09632876985045158 1106 3 0.12441460647938385 1106 4 0.08222590044499611 1106 5 -6.893834459447135E-4 1106 6 -0.07186531984626043 1106 7 -0.06345977555300664 1106 8 -0.07620989433192449 1106 9 0.07800540314833171 1106 10-11 -0.09639429139329536 1106 12-13 -0.21464192010420646 1106 14-15 0.05871430216814133 1106 16-17 0.11836828412265987 1106 18-19 0.020316160604096467 1106 20-21 0.1826764920822228 1106 22-23 0.1384448713815729 1106 24-25 0.10816264784038054 1106 26-27 0.06457730007706175 1106 28-29 0.07280308285652382 1106 30-31 0.09946243271677702 1106 32-33 0.07253185332763934 1106 34-35 -0.2715821463020802 1106 36-37 0.1240877573617496 1106 38-39 0.14977547479194442 1106 40-41 0.053752438793978286 1106 42-43 -0.12855895622588065 1106 44-45 0.04188015127372324 1106 46-47 0.102985796031561 1106 48-49 0.20981264232599273 1106 50-51 0.2990013013242603 1106 52-53 0.31784495495266185 1106 54-55 0.22947151378200203 1106 56-57 0.2700460341074873 1106 58-59 0.3319280482321645 1106 60-61 0.38589709069879063 1106 62-63 0.19265097551041777 1106 64-65 -0.0024580217184109188 1106 66-67 0.07238478959656902 1106 68-69 0.5290960144098165 1106 70-71 0.28402413821727635 1106 72-73 0.31921871022996484 1106 74-75 0.23148029248005386 1106 76-77 0.22724662833719833 1106 78-79 0.2445758196475758 1106 80-81 0.05401478970327034 1106 82-83 -0.05081936278511634 1106 84-85 -0.17640376610454567 1106 86-87 0.21742102478198788 1106 88-89 0.1685061546954998 1106 90-91 0.043974689822096735 1106 92-93 -0.03855355241624281 1106 94-95 0.1942264461431087 1106 96-97 0.27693995858238196 1106 98-99 0.08516202320662813 1106 100-101 -0.07283491061208025 1107 1 0.19954636102738021 1107 2 0.21505832168097072 1107 3 0.1390466586233856 1107 4 0.041774519541789346 1107 5 -0.02311329477947055 1107 6 0.057362175431642015 1107 7 3.1110371224229993E-4 1107 8 0.10166284703602457 1107 9 0.15708632586341054 1107 10-11 0.11967290535839226 1107 12-13 0.10470049133947867 1107 14-15 0.180905432285968 1107 16-17 0.14182146943209517 1107 18-19 0.20968902494723096 1107 20-21 0.16149031394735403 1107 22-23 0.13819650560736108 1107 24-25 0.012642851355394669 1107 26-27 0.07916577006618297 1107 28-29 0.12577041850401827 1107 30-31 0.15969738148832135 1107 32-33 0.14455614255520288 1107 34-35 0.18728837647164198 1107 36-37 0.2648259823561503 1107 38-39 0.4017001782705094 1107 40-41 0.3556211365780939 1107 42-43 0.42564201694788295 1107 44-45 0.36053659010128314 1107 46-47 0.23940025119266295 1107 48-49 0.22918496267983102 1107 50-51 0.4096936736910308 1107 52-53 0.42483704484405393 1107 54-55 0.3431651738701831 1107 56-57 0.34134801475364895 1107 58-59 0.34913869370383566 1107 60-61 0.20503576869555218 1107 62-63 0.07735611755359173 1107 64-65 0.12743423269299115 1107 66-67 0.08099021734964396 1107 68-69 0.1968789093276726 1107 70-71 0.14481632947131828 1107 72-73 0.12960052561259872 1107 74-75 0.00917601678711577 1107 76-77 0.02851095190119679 1107 78-79 0.15634379959684352 1107 80-81 0.2130372514208112 1107 82-83 0.14598147600858624 1107 84-85 0.32619205005059726 1107 86-87 0.0704249274326827 1107 88-89 0.07535607224646412 1107 90-91 0.15139398984858232 1107 92-93 0.2292403063015449 1107 94-95 0.1984083376842456 1107 96-97 0.08392772176346242 1107 98-99 0.2812025371778084 1107 100-101 0.12455255448806568 1108 1 0.17316656943844322 1108 2 0.06616938140766138 1108 3 -0.005428081800204154 1108 4 -0.0158860961922187 1108 5 -0.06370101872095546 1108 6 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54-55 -0.3183958106889193 2316 56-57 -0.4651784580035496 2316 58-59 -0.4716056672710991 2316 60-61 -0.28095908642197287 2316 62-63 -0.38482674088671587 2316 64-65 -0.4991624557887846 2316 66-67 -0.334025406255563 2316 68-69 -0.2955349191975998 2316 70-71 -0.04928007614237373 2316 72-73 -0.17002690347563743 2316 74-75 -0.07085632019984445 2316 76-77 -0.10710084202072778 2316 78-79 -0.2225482899710407 2316 80-81 -0.3221821471324624 2316 82-83 -0.1506475513554193 2316 84-85 -0.07889997352421219 2316 86-87 -0.20368476776913624 2316 88-89 -0.13617002998670458 2316 90-91 -0.19265453193112236 2316 92-93 -0.08299242599948897 2316 94-95 -0.03157820025864666 2316 96-97 0.06829530358341529 2316 98-99 -0.029707585443848217 2316 100-101 -0.04291075463074989 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 19.0 5 31.0 6 71.0 7 240.0 8 562.0 9 1145.0 10 1898.0 11 3125.0 12 5014.0 13 6897.0 14 8997.0 15 12733.0 16 17262.0 17 24021.0 18 21706.0 19 13201.0 20 11275.0 21 11328.0 22 12269.0 23 13243.0 24 15574.0 25 18360.0 26 22903.0 27 28025.0 28 35292.0 29 44251.0 30 54898.0 31 69297.0 32 88081.0 33 115379.0 34 155872.0 35 220674.0 36 332457.0 37 473928.0 38 335189.0 39 25053.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48744020117498 20.266096816366012 15.598197352941712 21.6482656295173 2 15.456274161719563 21.385479471230454 39.80906049144699 23.34918587560299 3 19.72546196435178 24.953846741184524 30.508599367898054 24.812091926565643 4 12.86369852523114 17.28155136830499 37.59544820947545 32.259301896988426 5 13.022376454796941 38.45797435155933 34.272187756334354 14.247461437309369 6 31.141428492217806 37.28028912710121 17.015258980910595 14.563023399770392 7 27.68245648958904 33.837542346921616 20.653662855330097 17.826338308159244 8 26.081976913826345 35.217140743124276 19.447497089004205 19.25338525404517 9 25.923360593525004 17.3063131113432 21.063701950389905 35.7066243447419 10-11 24.77009723316781 27.406218668731857 26.857333482405405 20.966350615694925 12-13 25.925742439254968 24.719068439854922 27.82858675199302 21.52660236889709 14-15 22.59669005462037 25.963673681234827 27.074533308972775 24.365102955172027 16-17 21.206047599439707 29.42794046934069 27.570629711336476 21.795382219883123 18-19 21.149872879699746 27.953922186196472 30.22920285527556 20.66700207882822 20-21 22.129544539371842 26.42616069423955 30.488533721647904 20.955761044740708 22-23 21.806448504768976 26.423035074913802 30.50507166873763 21.265444751579583 24-25 21.934482675558897 26.550909818917923 30.544187016792918 20.970420488730262 26-27 21.53261498941482 26.598253104425595 30.83916525697581 21.029966649183773 28-29 21.254953683809138 27.416235334380335 30.498716854148594 20.830094127661933 30-31 21.52707521504101 26.648837101493537 30.417156377512296 21.406931305953155 32-33 21.409635509694684 26.787160531532834 30.463400533932532 21.33980342483995 34-35 22.028898166504415 26.739462013436484 30.304352450008594 20.92728737005051 36-37 21.87721278856581 26.423570112503963 30.495941876435612 21.20327522249461 38-39 21.49530650217836 27.05969427499616 30.401521989494057 21.043477233331426 40-41 21.777040896674013 26.977302047094792 30.07113462949568 21.17452242673551 42-43 21.49555585908656 27.025499407622117 29.746710444827723 21.732234288463598 44-45 21.80260857946132 26.892579028650015 29.689157461011746 21.615654930876914 46-47 22.13645275733339 27.498888776781843 29.15522493066785 21.209433535216913 48-49 22.158745394900098 27.44996314102127 28.918891158299086 21.472400305779544 50-51 21.7971319987747 27.74406234121325 29.097073188024424 21.36173247198763 52-53 22.293546542755053 28.00925610309243 28.22925103100608 21.46794632314644 54-55 21.929132462593294 27.717706976494743 28.47843041366666 21.874730147245298 56-57 22.22339631628367 27.811962851966886 28.422749307133007 21.541891524616435 58-59 22.680474921805335 27.475050879934265 28.275836053444152 21.568638144816248 60-61 22.12438610030187 27.704294639131966 28.94741028179166 21.2239089787745 62-63 21.882871164461946 27.759696729231486 29.015878902575036 21.34155320373153 64-65 21.84294439157234 28.08942779048829 28.63769941798264 21.429928399956733 66-67 21.960679514134124 27.699522514882208 28.369983247571444 21.969814723412224 68-69 21.925433967889514 27.71815652385744 28.573093945401446 21.783315562851605 70-71 22.31147609529341 27.662481740943644 28.346145350723862 21.67989681303908 72-73 22.24721148873006 27.433237981745943 28.544711168316006 21.77483936120799 74-75 21.873513681796954 28.451683935321793 28.037327109875914 21.637475273005336 76-77 22.041351138356152 27.804077484754195 28.144352668162842 22.010218708726807 78-79 22.129499090569464 27.81116749792526 28.226774071276452 21.832559340228823 80-81 22.188809777673548 27.917904010311425 28.084792012645675 21.808494199369353 82-83 22.212427931861736 27.815648276352604 28.317884033329676 21.654039758455983 84-85 21.998952404867115 27.97756192116676 28.1916485534211 21.83183712054502 86-87 21.979608940324812 28.12028752730337 27.994621588726513 21.905481943645302 88-89 22.032829487910163 28.048592041551114 27.83027871932547 22.088299751213256 90-91 22.14217435158043 27.604689629968686 28.04425721237653 22.208878806074356 92-93 22.263253099661856 27.834031741991783 28.04914463876668 21.853570519579684 94-95 22.046850443443965 27.744016892410865 28.11869681922012 22.09043584492505 96-97 21.683328197618664 27.744380482829893 28.396798040974826 22.175493278576617 98-99 21.80424251211111 27.923743314973326 27.815801182863968 22.4562129900516 100-101 21.755250055969537 27.637358328891757 28.253170944027055 22.35422067111165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 515.0 1 381.0 2 312.0 3 406.5 4 378.5 5 374.0 6 701.5 7 1618.0 8 2714.0 9 3312.5 10 3226.0 11 3044.0 12 3215.0 13 3495.0 14 3799.0 15 4033.5 16 4211.5 17 4415.0 18 4599.5 19 4841.5 20 5303.0 21 6056.5 22 7130.0 23 8485.0 24 10076.5 25 12233.0 26 15414.0 27 19190.0 28 21870.0 29 24546.0 30 28498.0 31 32574.0 32 36707.0 33 40515.5 34 45100.0 35 49392.5 36 54151.0 37 58896.5 38 62082.5 39 64730.0 40 66375.5 41 69108.5 42 71968.0 43 75419.0 44 79678.0 45 81861.5 46 82643.5 47 90041.5 48 102772.0 49 99079.5 50 84421.0 51 75885.5 52 70485.5 53 67235.0 54 65310.0 55 60751.0 56 53706.5 57 47232.5 58 42464.5 59 37765.0 60 30903.0 61 24264.5 62 18841.0 63 13980.5 64 10219.5 65 7486.0 66 5356.5 67 3888.5 68 2772.5 69 1889.0 70 1276.0 71 866.0 72 602.0 73 458.5 74 333.5 75 209.5 76 144.5 77 96.5 78 61.0 79 40.0 80 29.0 81 22.0 82 14.0 83 7.0 84 6.5 85 5.5 86 4.0 87 3.0 88 3.0 89 1.5 90 1.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018270418556200624 2 0.0 3 0.0 4 4.5448802378608525E-5 5 0.04553969998336574 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004453982633103635 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.271808380577364E-4 24-25 2.272440118930426E-4 26-27 0.0014543616761154728 28-29 9.771492511400832E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.13558832111215E-4 42-43 0.011203129786327001 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.817952095144341E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.2724401189304262E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.499052392470406E-4 84-85 2.2724401189304262E-5 86-87 0.0 88-89 0.0 90-91 0.012498420654117343 92-93 0.0017270544903871237 94-95 0.0 96-97 0.0 98-99 0.0011362200594652132 100-101 0.019111221400204884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2200278.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.18292483474845 #Duplication Level Percentage of deduplicated Percentage of total 1 71.23037724247952 35.033182898676465 2 15.009167214047494 14.763894858413376 3 5.5863381399527965 8.242573465163606 4 2.7478786226816596 5.4059483101745665 5 1.4749372391991662 3.627086368575199 6 0.943403993777657 2.7839620628860797 7 0.608737783682429 2.095765326124699 8 0.419574631876066 1.650872606570224 9 0.2995717870916418 1.3260435018425534 >10 1.5306695406829895 13.50657870913294 >50 0.09768462234420969 3.2730313940451214 >100 0.04768058081424602 4.20004443161687 >500 0.002220614718149618 0.799372498348478 >1k 0.0013878841988434964 1.524978469324584 >5k 1.850512265124662E-4 0.7531830934854582 >10k+ 1.850512265124662E-4 1.0134820056198803 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12165 0.5528846809357726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10106 0.4593055968382177 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9640 0.4381264549297862 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6911 0.3140966732385635 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3628 0.1648882550295917 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3567 0.1621158780844966 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2692 0.12234817600321414 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2624 0.11925765744146875 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2545 0.11566720205355868 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2428 0.11034969217526149 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2390 0.10862263768487436 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.089760475721705E-5 0.0 0.0 0.0 0.0 3 9.089760475721705E-5 0.0 0.0 0.0 0.0 4 9.089760475721705E-5 0.0 0.0 0.0 0.0 5 9.089760475721705E-5 0.0 0.0 0.0 0.0 6 1.3634640713582557E-4 0.0 0.0 0.0 0.0 7 2.7269281427165113E-4 0.0 0.0 0.0 0.0 8 3.635904190288682E-4 0.0 0.0 0.0 0.0 9 3.635904190288682E-4 0.0 0.0 0.0 0.0 10-11 3.635904190288682E-4 0.0 0.0 0.0 0.0 12-13 4.544880237860852E-4 0.0 0.0 0.0 0.0 14-15 5.453856285433023E-4 0.0 0.0 0.0 2.2724401189304262E-5 16-17 5.681100297326065E-4 0.0 0.0 0.0 4.5448802378608525E-5 18-19 6.590076344898236E-4 0.0 0.0 0.0 4.5448802378608525E-5 20-21 7.271808380577364E-4 0.0 0.0 2.2724401189304262E-5 4.5448802378608525E-5 22-23 7.271808380577364E-4 0.0 0.0 4.5448802378608525E-5 4.5448802378608525E-5 24-25 8.180784428149534E-4 0.0 0.0 1.1362200594652131E-4 4.5448802378608525E-5 26-27 8.180784428149534E-4 0.0 0.0 2.9541721546095536E-4 4.5448802378608525E-5 28-29 0.0010680468558973003 0.0 0.0 7.953540416256492E-4 4.5448802378608525E-5 30-31 0.0010907712570866045 0.0 0.0 0.002363337723687643 4.5448802378608525E-5 32-33 0.0011134956582759088 0.0 0.0 0.0048402974533218075 4.5448802378608525E-5 34-35 0.001136220059465213 0.0 0.0 0.007158186374630842 9.089760475721705E-5 36-37 0.0012725664666010386 0.0 0.0 0.009271555685236138 9.089760475721705E-5 38-39 0.0014316372749261686 0.0 0.0 0.012293901043413605 9.089760475721705E-5 40-41 0.0014543616761154728 0.0 0.0 0.016225222449163242 9.089760475721705E-5 42-43 0.0016816056880085153 0.0 0.0 0.02181542514173209 9.089760475721705E-5 44-45 0.0018634008975229493 0.0 0.0 0.027269281427165115 9.089760475721705E-5 46-47 0.002249715717741122 0.0 0.0 0.03420022378990291 9.089760475721705E-5 48-49 0.002272440118930426 0.0 0.0 0.040835748937179756 9.089760475721705E-5 50-51 0.002272440118930426 0.0 0.0 0.05103900507117737 9.089760475721705E-5 52-53 0.00229516452011973 0.0 0.0 0.07305894982361319 9.089760475721705E-5 54-55 0.0023178889213090344 0.0 0.0 0.10657744157783698 9.089760475721705E-5 56-57 0.00245423532844486 0.0 0.0 0.13466480144781706 9.089760475721705E-5 58-59 0.00245423532844486 0.0 0.0 0.17109201655427178 9.089760475721705E-5 60-61 0.0024769597296341647 0.0 0.0 0.2170634801602343 9.089760475721705E-5 62-63 0.0025224085320127727 0.0 0.0 0.25040017670494363 9.089760475721705E-5 64-65 0.0025905817355806856 0.0 0.0 0.2778512533416232 9.089760475721705E-5 66-67 0.0026360305379592944 0.0 0.0 0.34847869223798084 9.089760475721705E-5 68-69 0.002681479340337903 0.0 0.0 0.42592345149112976 9.089760475721705E-5 70-71 0.0027269281427165113 0.0 0.0 0.465304838752194 9.089760475721705E-5 72-73 0.0027951013462844242 0.0 0.0 0.5423632831851248 9.089760475721705E-5 74-75 0.0028178257474737282 0.0 0.0 0.654485478653152 9.089760475721705E-5 76-77 0.0028178257474737282 0.0 0.0 0.7584723384954083 9.089760475721705E-5 78-79 0.0028178257474737282 0.0 0.0 0.880116058061754 9.089760475721705E-5 80-81 0.002954172154609554 0.0 0.0 1.029551720282619 1.1362200594652131E-4 82-83 0.0029996209569881625 0.0 0.0 1.196485171419248 1.3634640713582557E-4 84-85 0.0030905185617453794 0.0 0.0 1.352669980793336 1.3634640713582557E-4 86-87 0.0031814161665025963 0.0 0.0 1.5739374751735915 1.3634640713582557E-4 88-89 0.0031814161665025963 0.0 0.0 1.8420626848061925 1.3634640713582557E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9950 0.0 24.024874 1 TACATGG 9855 0.0 23.482222 2 GAGTACT 10645 0.0 22.668716 12-13 ACATGGG 9855 0.0 22.614296 3 GTACTTT 11045 0.0 21.738264 14-15 AGTACTT 11130 0.0 21.062063 12-13 AGAGTAC 16140 0.0 20.793016 10-11 GTATCAA 22655 0.0 20.411034 1 GGACCGA 640 0.0 20.037945 6 CATGGGG 4115 0.0 19.746635 4 GGTATCA 17990 0.0 18.544802 1 TACTTTT 12475 0.0 18.542053 14-15 ACTTTTT 13295 0.0 18.327297 16-17 TATCGTT 130 0.0075637344 18.269875 7 GTACCGT 210 1.9580608E-5 18.094229 6 CATGGGA 5910 0.0 18.09097 4 TGTACGC 185 1.5174963E-4 17.980143 2 TCAACGC 25735 0.0 17.873846 4 CAACGCA 25835 0.0 17.804663 5 TATCAAC 26045 0.0 17.734083 2 >>END_MODULE