FastQCFastQC Report
Thu 26 May 2016
SRR937718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137998
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105870.49518287669118494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81200.379794555467311No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72960.34125382717851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.2546775067142252No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49270.23044923334820705No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39300.18381682302789806No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31620.14789536753542334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30250.14148750373012509No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23310.10902723014708153No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22770.10650150280776689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97550.025.647521
TACATGG96400.025.5467552
ACATGGG98300.024.6648963
GAGTACT88150.023.57444212-13
GTACTTT93000.021.93565614-15
AGTACTT92250.021.88306812-13
GTATCAA192250.021.7227631
AGAGTAC139550.020.86538910-11
CATGGGG44750.020.3986474
CATGGGA54050.020.2313734
GTCGTAC1200.00472730319.8187241
GGTATCA149900.019.5146331
CAACGCA219700.018.8906445
TCAACGC220800.018.7763254
ATCAACG220700.018.740433
AACGCAG222700.018.643086
TATCAAC221650.018.6395342
TACTTTT108550.018.35576614-15
GAGTACA76850.018.1966041
ACTTTTT114150.018.1626316-17
Produced by FastQC (version 0.11.5)