FastQCFastQC Report
Thu 26 May 2016
SRR937718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137998
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121050.5661838785630295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98430.4603839666828501No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94790.4433586935067292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66820.31253537187593255No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36040.16856891353499862No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33810.15813859507819933No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26790.12530413966710915No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25840.12086073045905561No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25030.11707213945008367No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24170.1130496847985826No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23250.10874659377604655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100400.022.6721571
TACATGG100500.021.88742
GAGTACT102150.021.5063312-13
AGTACTT106700.021.16796112-13
ACATGGG102100.020.7068253
AGAGTAC159600.020.65497210-11
GTATCAA210650.020.2584381
GTACTTT108900.019.9317614-15
CGCGGAA1152.0612479E-618.58627382-83
TACTTTT122500.018.4168314-15
CATGGGG42750.018.336954
GGTATCA167550.018.0953621
GTGTAAG8150.018.07571
CAACGCA245000.017.3748555
TCAACGC245900.017.3499034
CATGGGA59300.017.3052674
ATCAACG247150.017.2621543
TATCAAC247300.017.213262
GAGTACA82900.017.0825521
AACGCAG250200.017.0473376
Produced by FastQC (version 0.11.5)