##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937718_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2137998 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1053326523224 31.0 31.0 34.0 31.0 34.0 2 32.30074069292862 34.0 31.0 34.0 31.0 34.0 3 32.391954529424254 34.0 31.0 34.0 31.0 34.0 4 36.04878816537714 37.0 35.0 37.0 35.0 37.0 5 35.95280678466491 37.0 35.0 37.0 35.0 37.0 6 35.99047239520336 37.0 35.0 37.0 35.0 37.0 7 35.932132303210764 37.0 35.0 37.0 35.0 37.0 8 35.90887549941581 37.0 35.0 37.0 35.0 37.0 9 37.555210996455564 39.0 37.0 39.0 35.0 39.0 10-11 37.4893084558545 39.0 37.0 39.0 35.0 39.0 12-13 37.39558689952002 39.0 37.0 39.0 34.0 39.0 14-15 38.551534893858644 40.0 38.0 41.0 34.0 41.0 16-17 38.52392448449437 40.0 38.0 41.0 34.0 41.0 18-19 38.53056176853299 40.0 38.0 41.0 34.0 41.0 20-21 38.42307219183554 40.0 38.0 41.0 34.0 41.0 22-23 38.262768721018446 40.0 38.0 41.0 34.0 41.0 24-25 38.33910111234903 40.0 38.0 41.0 34.0 41.0 26-27 38.208132561396226 40.0 38.0 41.0 34.0 41.0 28-29 38.068367229529684 40.0 37.0 41.0 33.0 41.0 30-31 38.10918064469658 40.0 37.0 41.0 34.0 41.0 32-33 38.02346026516395 40.0 37.0 41.0 33.0 41.0 34-35 37.88492856401175 40.0 37.0 41.0 33.0 41.0 36-37 37.716465122979535 40.0 37.0 41.0 33.0 41.0 38-39 37.56914997114123 40.0 37.0 41.0 33.0 41.0 40-41 37.40202306082607 40.0 36.5 41.0 32.5 41.0 42-43 37.169654040836335 40.0 36.0 41.0 32.0 41.0 44-45 36.83318225742026 39.0 36.0 41.0 31.0 41.0 46-47 36.46220599832179 39.0 36.0 41.0 30.0 41.0 48-49 36.10769841693023 39.0 35.0 41.0 29.5 41.0 50-51 35.83975195486619 39.0 35.0 40.0 28.5 41.0 52-53 35.60759528306387 39.0 35.0 40.0 28.0 41.0 54-55 35.39010583733007 38.5 35.0 40.0 28.0 41.0 56-57 35.11071829814621 38.0 34.5 40.0 27.0 41.0 58-59 34.839403731902465 38.0 34.0 40.0 26.0 41.0 60-61 34.4673771911854 38.0 34.0 40.0 26.0 41.0 62-63 34.12429127623132 37.0 33.5 40.0 25.5 41.0 64-65 33.7264113436963 37.0 33.0 39.5 24.5 41.0 66-67 33.2717009089812 36.0 32.5 39.0 23.0 40.0 68-69 32.82080455641212 35.5 32.0 39.0 22.0 40.0 70-71 32.38714512361564 35.0 31.5 38.0 21.5 40.0 72-73 31.879258539998634 35.0 31.0 37.5 20.0 39.5 74-75 31.37518323216392 35.0 31.0 37.0 18.5 39.0 76-77 30.141130627811627 33.5 29.5 35.5 18.0 38.0 78-79 30.758456509313852 34.0 30.5 36.0 18.0 38.0 80-81 30.559287941335775 34.0 30.0 35.5 17.5 37.0 82-83 30.192993164633457 34.0 30.0 35.0 12.5 37.0 84-85 29.789760794911874 34.0 30.0 35.0 7.5 36.5 86-87 29.37969563114652 34.0 29.0 35.0 4.5 36.0 88-89 28.981213733595634 34.0 29.0 35.0 2.0 36.0 90-91 28.433600265294917 33.5 29.0 35.0 2.0 35.0 92-93 27.933907094393916 33.0 27.0 35.0 2.0 35.0 94-95 27.42391643958507 33.0 26.5 35.0 2.0 35.0 96-97 26.819169381823556 33.0 25.5 34.5 2.0 35.0 98-99 25.89514513109928 32.0 24.5 34.0 2.0 35.0 100-101 23.234644980958823 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3668153300884214 1101 2 0.33941268028435445 1101 3 0.35059845782157595 1101 4 0.20412644679064584 1101 5 0.19286896732557324 1101 6 0.19993860278447784 1101 7 0.24658720223712294 1101 8 0.2630644588854878 1101 9 0.39297474913743 1101 10-11 0.3553616291019921 1101 12-13 0.3426665350639979 1101 14-15 0.39113771696492705 1101 16-17 0.48065346727310754 1101 18-19 0.4443150494548007 1101 20-21 0.5101926524047613 1101 22-23 0.4493982605616651 1101 24-25 0.48776518759231635 1101 26-27 0.45513194890448716 1101 28-29 0.42113291956591326 1101 30-31 0.5098038430932021 1101 32-33 0.49434195460797525 1101 34-35 0.5234658421255247 1101 36-37 0.456776512455356 1101 38-39 0.39181609805503825 1101 40-41 0.36016511699927634 1101 42-43 0.47637306897185283 1101 44-45 0.5254134507077453 1101 46-47 0.6550806092646866 1101 48-49 0.5567829106196243 1101 50-51 0.5604637962833294 1101 52-53 0.6269726707803756 1101 54-55 0.5982234898676353 1101 56-57 0.666922139190369 1101 58-59 0.7776123520888873 1101 60-61 0.7301510585158226 1101 62-63 0.6867424234028974 1101 64-65 0.7208702322774414 1101 66-67 0.6781293250086762 1101 68-69 0.7195099592923313 1101 70-71 0.7609336062843113 1101 72-73 0.688173231193133 1101 74-75 0.694035206418409 1101 76-77 0.5811773051182563 1101 78-79 0.6617688806787889 1101 80-81 0.5877592557443592 1101 82-83 0.5085865723630931 1101 84-85 0.577669890605172 1101 86-87 0.5553828415062476 1101 88-89 0.7862429011495244 1101 90-91 0.7044202460871496 1101 92-93 0.6724728108757603 1101 94-95 0.8399926364553068 1101 96-97 0.8488305667930582 1101 98-99 0.9082996463709812 1101 100-101 1.4895250770762658 1102 1 0.248662614811046 1102 2 0.2419113978084937 1102 3 0.17724538410031698 1102 4 0.18762309287276935 1102 5 0.1685999785659078 1102 6 0.18268004915750424 1102 7 0.2189103991410022 1102 8 0.13414414122109264 1102 9 0.3214015571037123 1102 10-11 0.29982977499903996 1102 12-13 0.3370203945046413 1102 14-15 0.37524832807279296 1102 16-17 0.3141329054512738 1102 18-19 0.24558810966947675 1102 20-21 0.3194560242448361 1102 22-23 0.3295604694071841 1102 24-25 0.3330281438452687 1102 26-27 0.2712268586116906 1102 28-29 0.30522892018928616 1102 30-31 0.35560024436853155 1102 32-33 0.3505079037375083 1102 34-35 0.34717086618412196 1102 36-37 0.35920704771857714 1102 38-39 0.29569956483079096 1102 40-41 0.32800581726356626 1102 42-43 0.5765854080130168 1102 44-45 0.5494856849430292 1102 46-47 0.5660680182738389 1102 48-49 0.5497887093585732 1102 50-51 0.5044559650636415 1102 52-53 0.5296019086325714 1102 54-55 0.5262239963644291 1102 56-57 0.43560375400604556 1102 58-59 0.5054031169639828 1102 60-61 0.5087632572963301 1102 62-63 0.5507973847987486 1102 64-65 0.6389421028739974 1102 66-67 0.6469537978948594 1102 68-69 0.6235577128491698 1102 70-71 0.5784385900829108 1102 72-73 0.6867508193592897 1102 74-75 0.568488611960003 1102 76-77 0.48992086838814686 1102 78-79 0.6524775865794759 1102 80-81 0.6251543037641518 1102 82-83 0.6516271115873771 1102 84-85 0.533804346023711 1102 86-87 0.7636991292743467 1102 88-89 0.7624651493459425 1102 90-91 0.9046549878711865 1102 92-93 0.7616139741300678 1102 94-95 0.8432523978823276 1102 96-97 0.808724137530529 1102 98-99 1.116017553630119 1102 100-101 1.562755098050431 1103 1 0.2642547006543481 1103 2 0.2629152869006077 1103 3 0.23694539071009046 1103 4 0.16221364615184797 1103 5 0.107929649017386 1103 6 0.1829703044220281 1103 7 0.26888788075166303 1103 8 0.16256821935271404 1103 9 0.24303181459338674 1103 10-11 0.14142532502044958 1103 12-13 0.21733156292782496 1103 14-15 0.2912012623810867 1103 16-17 0.3272498159200481 1103 18-19 0.3126851095302996 1103 20-21 0.15480759644717068 1103 22-23 0.17531935554952582 1103 24-25 0.2931747001738856 1103 26-27 0.29862857723487224 1103 28-29 0.23782801444506418 1103 30-31 0.3772177954926619 1103 32-33 0.46365808887274795 1103 34-35 0.40091725287556557 1103 36-37 0.2823711010614929 1103 38-39 0.2382176298340184 1103 40-41 0.2468536064996485 1103 42-43 0.44441683169661417 1103 44-45 0.45439455012704855 1103 46-47 0.2674636041768679 1103 48-49 0.12274326162442861 1103 50-51 0.10897403253013493 1103 52-53 0.1450632564433647 1103 54-55 0.16499906167186396 1103 56-57 0.13356790275961572 1103 58-59 0.00862209711290518 1103 60-61 0.10977116562329314 1103 62-63 0.20998813095183522 1103 64-65 0.050939128886540175 1103 66-67 0.06485230461979086 1103 68-69 0.0869099066165262 1103 70-71 0.041946277741971016 1103 72-73 0.027364448547718467 1103 74-75 0.06831854372333268 1103 76-77 0.0798075251442576 1103 78-79 0.23521881148953483 1103 80-81 0.29568152966690775 1103 82-83 0.32758184408245583 1103 84-85 0.34990033471238036 1103 86-87 0.2895508355195986 1103 88-89 0.2753565680126826 1103 90-91 0.33289778988959284 1103 92-93 0.4528090581008719 1103 94-95 0.5855193736072195 1103 96-97 0.6363167184910132 1103 98-99 0.7101428815015609 1103 100-101 1.1107373313081013 1104 1 0.19038542448995344 1104 2 0.17587085586111328 1104 3 0.09170351108019048 1104 4 -0.008516796179719677 1104 5 0.04647591366309456 1104 6 0.08504512002411246 1104 7 0.12186106765183524 1104 8 0.1840612280308278 1104 9 0.18356488493429168 1104 10-11 0.19175354691712698 1104 12-13 0.24805045146563032 1104 14-15 0.2003368183834695 1104 16-17 0.21005361367121367 1104 18-19 0.2120184923343018 1104 20-21 0.1066544879340725 1104 22-23 0.3153323056926638 1104 24-25 0.21647562224323025 1104 26-27 0.11699902952440056 1104 28-29 0.16534451530521466 1104 30-31 0.27786633993806475 1104 32-33 0.2729059911570175 1104 34-35 0.23424440237591426 1104 36-37 0.17145607729961654 1104 38-39 0.14015460580403527 1104 40-41 0.1516898027481801 1104 42-43 0.17608216584702063 1104 44-45 0.2715787038754911 1104 46-47 0.15062367049947767 1104 48-49 0.13467805928942056 1104 50-51 0.10907995560484807 1104 52-53 0.08967586124935423 1104 54-55 0.13436993845161282 1104 56-57 -0.008284837436413284 1104 58-59 0.022206980793008313 1104 60-61 0.05245720723609537 1104 62-63 -0.07469619035853015 1104 64-65 0.12925278566225984 1104 66-67 0.0297087838405119 1104 68-69 0.05766496459294501 1104 70-71 0.17143893214629458 1104 72-73 0.04835736466781526 1104 74-75 -0.017815467517682038 1104 76-77 -0.04264393775114783 1104 78-79 0.1431517117151877 1104 80-81 0.22789694568327334 1104 82-83 0.25869573991319683 1104 84-85 0.33372143283345324 1104 86-87 0.30848433577652656 1104 88-89 0.3390408717689404 1104 90-91 0.3885259556131224 1104 92-93 0.4002714125214766 1104 94-95 0.37374623330522283 1104 96-97 0.7361620827706439 1104 98-99 1.0036906302671902 1104 100-101 1.1907044652331678 1105 1 0.26592448769159205 1105 2 0.2260639470344401 1105 3 0.15317794384935723 1105 4 0.13472950324631228 1105 5 0.06011313086413139 1105 6 0.1674148345104456 1105 7 0.19887954398694063 1105 8 0.17192689699172803 1105 9 0.25262375937195714 1105 10-11 0.2916193424282625 1105 12-13 0.30466617145485486 1105 14-15 0.31675344626861346 1105 16-17 0.2675303855786879 1105 18-19 0.2751132784643957 1105 20-21 0.4066180537116182 1105 22-23 0.3563624450612224 1105 24-25 0.428520959887436 1105 26-27 0.2762480150813431 1105 28-29 0.41475799576341643 1105 30-31 0.3754320848291073 1105 32-33 0.4472834456193411 1105 34-35 0.3943209652713833 1105 36-37 0.43944305078938584 1105 38-39 0.4127145334662927 1105 40-41 0.3974821093100118 1105 42-43 0.36685185665620423 1105 44-45 0.4880654446761099 1105 46-47 0.5046870558408756 1105 48-49 0.5211182010256863 1105 50-51 0.5131737538266137 1105 52-53 0.6267470192248794 1105 54-55 0.6045508236501291 1105 56-57 0.5685701441525666 1105 58-59 0.5945281979613881 1105 60-61 0.49629370024317865 1105 62-63 0.4626993036364624 1105 64-65 0.5565557959250782 1105 66-67 0.41683872644385644 1105 68-69 0.2813775200097126 1105 70-71 0.36242814679999924 1105 72-73 0.42182151420937686 1105 74-75 0.43950934204461944 1105 76-77 0.49808299132356737 1105 78-79 0.6664364456739236 1105 80-81 0.46035595973443577 1105 82-83 0.6204733829762041 1105 84-85 0.5544015791418602 1105 86-87 0.5853281931911454 1105 88-89 0.5590796941168961 1105 90-91 0.6421644791392218 1105 92-93 0.6858723424616322 1105 94-95 0.8034477788510621 1105 96-97 0.8015330382657808 1105 98-99 1.0334630031250214 1105 100-101 1.134730783600915 1106 1 0.08456824205418911 1106 2 0.08055406952390598 1106 3 0.08370795478457893 1106 4 0.03929717710087033 1106 5 -0.02446777642804676 1106 6 0.017775012766342968 1106 7 0.0362726636683135 1106 8 0.07809498302831486 1106 9 0.197313120992213 1106 10-11 -0.014345004253591753 1106 12-13 -0.0933902876492283 1106 14-15 0.10890638119494866 1106 16-17 0.22349499949474705 1106 18-19 0.06140779834608168 1106 20-21 0.08882784538652544 1106 22-23 0.06309920547721504 1106 24-25 0.22717217542891888 1106 26-27 0.053131080877612646 1106 28-29 0.10391822832130515 1106 30-31 0.20074023258610652 1106 32-33 0.17875668638987463 1106 34-35 0.07231273232012114 1106 36-37 0.01652782496045546 1106 38-39 -0.03941249043193551 1106 40-41 0.0414119913314579 1106 42-43 0.07461586534024889 1106 44-45 0.1000950456740668 1106 46-47 0.3471889451866801 1106 48-49 0.44666643198872436 1106 50-51 0.4441348669746432 1106 52-53 0.3063612185538034 1106 54-55 0.39527715551867004 1106 56-57 0.2873936936854946 1106 58-59 0.26981630315332694 1106 60-61 0.29739780506739777 1106 62-63 0.27740210179232605 1106 64-65 0.315219795950739 1106 66-67 0.3731368486425808 1106 68-69 0.3515880684918713 1106 70-71 0.38029093593404184 1106 72-73 0.290435498682001 1106 74-75 0.26345235061000594 1106 76-77 0.09350526946306204 1106 78-79 0.055749643088379486 1106 80-81 0.11138016153259755 1106 82-83 -0.08700169208988129 1106 84-85 -0.011425687084337 1106 86-87 0.14739258808205236 1106 88-89 0.07588573232710871 1106 90-91 0.12176745463170846 1106 92-93 0.2784823547650923 1106 94-95 0.2725398987558414 1106 96-97 0.35743383665468187 1106 98-99 0.5468170168223772 1106 100-101 0.6937789595137076 1107 1 0.2515702215352391 1107 2 0.3335405609354396 1107 3 0.2658197347215534 1107 4 0.12097304243899032 1107 5 0.11628833292696328 1107 6 0.23521659901907555 1107 7 0.23077251717241865 1107 8 0.11189313313062144 1107 9 0.34413654223101986 1107 10-11 0.34656655748787557 1107 12-13 0.3032930692266689 1107 14-15 0.3274809880976406 1107 16-17 0.3769738600187509 1107 18-19 0.2971355256112318 1107 20-21 0.3520934650641365 1107 22-23 0.42834537812321116 1107 24-25 0.36278723187152195 1107 26-27 0.3676781227325634 1107 28-29 0.43231115650020513 1107 30-31 0.3485493448686583 1107 32-33 0.3978714224471531 1107 34-35 0.35451371991680247 1107 36-37 0.4050975724872927 1107 38-39 0.5535925094846945 1107 40-41 0.6850715777604606 1107 42-43 0.46502086096919015 1107 44-45 0.46427811074920555 1107 46-47 0.41763989050202355 1107 48-49 0.4602420750818581 1107 50-51 0.47645960191236725 1107 52-53 0.5067598541172842 1107 54-55 0.5099403221642973 1107 56-57 0.5407895398719589 1107 58-59 0.40052648664239854 1107 60-61 0.5550491781983808 1107 62-63 0.6939383910871655 1107 64-65 0.6790289987510647 1107 66-67 0.5934769096706205 1107 68-69 0.6850672625024572 1107 70-71 0.7578497903599981 1107 72-73 0.8349535748455708 1107 74-75 0.6620710015615181 1107 76-77 0.5596835370955695 1107 78-79 0.7967025095246214 1107 80-81 0.7477673585064473 1107 82-83 0.7176092362652859 1107 84-85 0.8210852048255184 1107 86-87 0.9058177691771796 1107 88-89 0.7531872065759941 1107 90-91 1.1335043956546045 1107 92-93 1.1605367273696494 1107 94-95 1.1573456547341863 1107 96-97 1.31234788048911 1107 98-99 1.4878008498694655 1107 100-101 1.6438886527775125 1108 1 0.04775038856288916 1108 2 0.08162800312648244 1108 3 0.0336157669745063 1108 4 0.02485434619590876 1108 5 -0.05863831033597933 1108 6 -0.005808906554115367 1108 7 0.009068047574047 1108 8 -0.04075803457416782 1108 9 0.12205652455816818 1108 10-11 0.04978801079168704 1108 12-13 0.04029824141690597 1108 14-15 0.13686128575071166 1108 16-17 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-0.09009485520687832 2316 78-79 -0.06426693621260782 2316 80-81 -0.09101588264818616 2316 82-83 -0.07246766349984668 2316 84-85 -0.0118185324705955 2316 86-87 -0.09572780245703782 2316 88-89 0.08174727065011211 2316 90-91 -0.00517088277558031 2316 92-93 0.24885348296801268 2316 94-95 0.21281414260664278 2316 96-97 0.31805962272066424 2316 98-99 0.26472226166473334 2316 100-101 0.7294952463506199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 19.0 5 44.0 6 77.0 7 239.0 8 520.0 9 1122.0 10 1854.0 11 3085.0 12 4780.0 13 6780.0 14 9000.0 15 12496.0 16 16896.0 17 23685.0 18 21158.0 19 13012.0 20 10940.0 21 11239.0 22 11986.0 23 13082.0 24 15021.0 25 18212.0 26 22301.0 27 27373.0 28 34576.0 29 43398.0 30 54436.0 31 68305.0 32 86601.0 33 112617.0 34 152477.0 35 214492.0 36 319399.0 37 453345.0 38 326711.0 39 26714.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.5602824901839 20.33247238324427 15.57134509845567 21.535900028116156 2 15.423120133882259 21.452732883753868 39.8282411863809 23.29590579598297 3 19.813863249638214 24.984448067771815 30.488522440151954 24.71316624243802 4 12.872674104791686 17.29284553651694 37.63345453729056 32.201025821400805 5 13.04131597292075 38.433773269767904 34.277099133071026 14.247811624240326 6 31.137213411799262 37.315048938305836 16.97097003832557 14.576767611569327 7 27.643150274228507 33.89867530278326 20.675557226901052 17.78261719608718 8 26.087863505952765 35.20527147359352 19.475883513455113 19.230981506998603 9 25.935805365580322 17.357593412154735 21.048710054920537 35.6578911673444 10-11 24.755097993543494 27.424487768463766 26.885969958811934 20.934444279180802 12-13 25.914281698369184 24.750885380964643 27.827892651811588 21.506940268854585 14-15 22.601284004942944 25.999463049076756 27.083210554921006 24.3160423910593 16-17 21.22511340048026 29.465439163179756 27.52233631649796 21.78711111984202 18-19 21.09674564709602 27.98412346503598 30.29827436695451 20.62085652091349 20-21 22.14433783380527 26.46531942499478 30.52215203194765 20.868190709252303 22-23 21.84826468809475 26.403116409795636 30.538622330590705 21.20999657151891 24-25 21.950849322987825 26.535865111129713 30.596184185478275 20.917101380404183 26-27 21.58145933008759 26.623459235605928 30.849993521925594 20.945087912380885 28-29 21.260776882716883 27.43449093590057 30.49599678199037 20.80873539939218 30-31 21.51150281712144 26.686741521741368 30.430898438632774 21.370857222504416 32-33 21.377031222667185 26.784262660676017 30.477367144403317 21.36133897225348 34-35 22.02368758062449 26.76031502368103 30.326291231329495 20.88970616436498 36-37 21.866858621944456 26.45509958381626 30.5334476458818 21.14459414835748 38-39 21.49538633374431 27.06852585318214 30.433592953401224 21.00249485967233 40-41 21.776373963422092 27.01864770301188 30.092383738048618 21.112594595517415 42-43 21.471232482612717 27.0763520095804 29.7686178703956 21.68379763741128 44-45 21.80572666578734 26.86618509465397 29.75854046636152 21.56954777319717 46-47 22.200161085276974 27.48732692921135 29.173132996382595 21.13937898912908 48-49 22.145694242931942 27.467682383238902 28.970794172866395 21.41582920096277 50-51 21.793261733640538 27.77032532303585 29.12750619972516 21.30890674359845 52-53 22.28992316408228 28.04930782192215 28.26249796830913 21.398271045686442 54-55 21.93336944187974 27.755989481748816 28.481691750880966 21.82894932549048 56-57 22.19585799425444 27.867869848334752 28.44352520442021 21.4927469529906 58-59 22.656148415480278 27.530100589429924 28.305896450791817 21.507854544297984 60-61 22.1289262197626 27.742261685932352 29.035667947303974 21.093144147001073 62-63 21.84506253046074 27.812584483240865 29.027576265272465 21.314776721025932 64-65 21.812274847778156 28.112093650227926 28.690789233666266 21.38484226832766 66-67 21.99775210266801 27.66831400216464 28.371167793421698 21.96276610174565 68-69 21.89981223066185 27.771316637476552 28.608128501673647 21.72074263018795 70-71 22.27689695614705 27.742782673974126 28.341567284339668 21.638753085539157 72-73 22.254815018536032 27.47177499698316 28.575751707906182 21.69765827657463 74-75 21.870862805031344 28.494935096977432 28.09058476448172 21.54361733350951 76-77 22.070600627315834 27.80463311939487 28.166466947115946 21.958299306173345 78-79 22.09431440066829 27.892308598979042 28.210854266467976 21.80252273388469 80-81 22.189343577189305 27.939889532118144 28.10067086155874 21.77009602913381 82-83 22.175783883752764 27.88409795223064 28.38069272093661 21.55942544307998 84-85 21.993689869061626 27.96408590215906 28.257094687745337 21.785129541033974 86-87 21.943682828515275 28.14941828757557 28.018033693202703 21.888865190706447 88-89 22.038818558296125 28.109567922888605 27.87476882578936 21.976844693025903 90-91 22.124966788038456 27.636538583879382 28.096126643290436 22.14236798479173 92-93 22.245551981773165 27.859256534249106 28.096446753929534 21.798744730048202 94-95 22.06342101349019 27.806340323985335 28.14490471927476 21.985333943249714 96-97 21.69990336754291 27.787023187112432 28.411228635386937 22.101844809957726 98-99 21.81097743791181 27.968228695765653 27.813665973328867 22.40712789299367 100-101 21.712544521006947 27.626075702611487 28.303700222841865 22.357679553539704 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 468.0 1 348.5 2 290.0 3 381.5 4 355.0 5 355.5 6 695.0 7 1573.0 8 2682.5 9 3319.0 10 3218.0 11 3045.5 12 3181.0 13 3441.0 14 3797.5 15 3997.5 16 4125.0 17 4292.5 18 4474.5 19 4738.5 20 5154.5 21 5874.5 22 6945.5 23 8354.0 24 9890.0 25 12055.0 26 15298.0 27 18808.0 28 21108.5 29 23706.0 30 27843.0 31 31819.5 32 35500.5 33 39444.0 34 44104.0 35 48123.0 36 52928.0 37 57858.5 38 61039.0 39 63443.0 40 64984.5 41 67631.0 42 70142.5 43 73294.0 44 77430.5 45 79555.5 46 79987.0 47 86980.0 48 99597.0 49 96171.5 50 81923.5 51 73906.0 52 68416.5 53 64690.5 54 63232.0 55 58843.0 56 51801.5 57 45669.5 58 40929.5 59 36251.0 60 29698.5 61 23234.5 62 17985.5 63 13398.5 64 9827.5 65 7203.5 66 5065.0 67 3706.5 68 2744.5 69 1860.5 70 1219.5 71 816.5 72 589.5 73 447.5 74 328.0 75 212.0 76 131.5 77 80.0 78 55.5 79 38.0 80 26.5 81 18.0 82 11.5 83 7.5 84 4.5 85 3.0 86 0.5 87 1.5 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02043968235704617 2 0.0 3 0.0 4 1.870909140233059E-4 5 0.02656690979130944 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035781137306957257 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011693182126456618 24-25 4.677272850582648E-5 26-27 7.951363845990501E-4 28-29 0.0014265682194277076 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.354545701165295E-5 40-41 6.314318348286575E-4 42-43 0.014055204916000857 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.338636425291324E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.1693182126456621E-4 70-71 2.338636425291324E-5 72-73 0.0 74-75 2.338636425291324E-5 76-77 0.0 78-79 0.0 80-81 4.677272850582648E-5 82-83 0.0017539773189684928 84-85 3.507954637936986E-4 86-87 0.0 88-89 0.0 90-91 0.010009363900246867 92-93 0.002408795518050064 94-95 0.0 96-97 0.0 98-99 0.0014499545836806208 100-101 0.017937341381984457 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2137998.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.519153983271146 #Duplication Level Percentage of deduplicated Percentage of total 1 71.27498381497239 35.29476898688777 2 15.121640537009515 14.976216924636985 3 5.581227409451123 8.29132978512791 4 2.7034013329691757 5.354805875435244 5 1.5026581509077197 3.7205180179508432 6 0.917336598104679 2.7255439353621433 7 0.563264929524886 1.9524681944963442 8 0.40761310355614316 1.6147724832476562 9 0.29747237900928625 1.3257522487737756 >10 1.4856408836607347 13.327629786402925 >50 0.09535304662409842 3.2655567539614507 >100 0.045150544531929446 3.9839681251164367 >500 0.0024597558142195517 0.8501053917660191 >1k 0.0014190898928189576 1.5312297327164184 >5k 2.838179785637915E-4 1.2182376616049486 >10k+ 9.460599285459717E-5 0.5670960965131481 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12105 0.5661838785630295 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9843 0.4603839666828501 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9479 0.4433586935067292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6682 0.31253537187593255 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3604 0.16856891353499862 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3381 0.15813859507819933 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2679 0.12530413966710915 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2584 0.12086073045905561 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2503 0.11707213945008367 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2417 0.1130496847985826 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2325 0.10874659377604655 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.677272850582648E-5 0.0 0.0 0.0 0.0 6 9.354545701165295E-5 0.0 0.0 0.0 0.0 7 9.354545701165295E-5 0.0 0.0 0.0 0.0 8 9.354545701165295E-5 0.0 0.0 0.0 0.0 9 9.354545701165295E-5 0.0 0.0 0.0 0.0 10-11 9.354545701165295E-5 0.0 0.0 0.0 0.0 12-13 1.870909140233059E-4 0.0 0.0 0.0 0.0 14-15 3.274090995407853E-4 0.0 0.0 0.0 0.0 16-17 4.209545565524383E-4 0.0 0.0 0.0 0.0 18-19 5.846591063228309E-4 0.0 0.0 0.0 0.0 20-21 7.015909275873972E-4 0.0 0.0 0.0 0.0 22-23 7.015909275873972E-4 0.0 0.0 7.015909275873971E-5 0.0 24-25 7.015909275873972E-4 0.0 0.0 1.6370454977039265E-4 0.0 26-27 7.015909275873972E-4 0.0 0.0 3.507954637936986E-4 0.0 28-29 7.483636560932236E-4 0.0 0.0 0.0010290000271281825 0.0 30-31 7.483636560932236E-4 0.0 0.0 0.003063613717131634 0.0 32-33 7.951363845990502E-4 0.0 0.0 0.005472409235181698 0.0 34-35 9.354545701165296E-4 0.0 0.0 0.008255386581278373 0.0 36-37 0.001075772755634009 0.0 0.0 0.010991591198869222 0.0 38-39 0.001169318212645662 0.0 0.0 0.013891500366230464 0.0 40-41 0.001169318212645662 0.0 0.0 0.018802636859342248 0.0 42-43 0.0012394773054044018 0.0 0.0 0.02591209159222787 0.0 44-45 0.0013096363981631414 0.0 0.0 0.03168852356269744 0.0 46-47 0.0014031818551747943 0.0 0.0 0.03896168284535346 0.0 48-49 0.0014499545836806208 0.0 0.0 0.04609452394249199 0.0 50-51 0.0014499545836806208 0.0 0.0 0.05708611514136122 0.0 52-53 0.0014499545836806208 0.0 0.0 0.08304497946209491 0.0 54-55 0.001566886404945187 0.0 0.0 0.11941077587537499 0.0 56-57 0.0017305909547155798 0.0 0.0 0.1494388675761156 0.0 58-59 0.0017305909547155798 0.0 0.0 0.1933818460073396 0.0 60-61 0.0017305909547155798 0.0 0.0 0.24169807455385833 0.0 62-63 0.0017773636832214062 0.0 0.0 0.2771284163970219 0.0 64-65 0.0018709091402330591 0.0 0.0 0.3080685763036261 0.0 66-67 0.0019176818687388856 0.0 0.0 0.38648305564364416 0.0 68-69 0.001964454597244712 0.0 0.0 0.4693643305559687 0.0 70-71 0.002058000054256365 0.0 0.0 0.5092146952429328 0.0 72-73 0.0021281591470151046 0.0 0.0 0.589780720094219 4.677272850582648E-5 74-75 0.002151545511268018 0.0 0.0 0.7082326550352245 9.354545701165295E-5 76-77 0.002174931875520931 0.0 0.0 0.8214694307478305 9.354545701165295E-5 78-79 0.0022217046040267576 0.0 0.0 0.9491589795687367 9.354545701165295E-5 80-81 0.002712818253337936 0.0 0.0 1.1076249837464767 9.354545701165295E-5 82-83 0.0028063637103495887 0.0 0.0 1.2786962382565372 9.354545701165295E-5 84-85 0.0028999091673612416 0.0 0.0 1.4356421287578378 9.354545701165295E-5 86-87 0.0030168409886258078 0.0 0.0 1.6666994075766208 9.354545701165295E-5 88-89 0.003063613717131634 0.0 0.0 1.9408109829850169 9.354545701165295E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10040 0.0 22.672157 1 TACATGG 10050 0.0 21.8874 2 GAGTACT 10215 0.0 21.50633 12-13 AGTACTT 10670 0.0 21.167961 12-13 ACATGGG 10210 0.0 20.706825 3 AGAGTAC 15960 0.0 20.654972 10-11 GTATCAA 21065 0.0 20.258438 1 GTACTTT 10890 0.0 19.93176 14-15 CGCGGAA 115 2.0612479E-6 18.586273 82-83 TACTTTT 12250 0.0 18.41683 14-15 CATGGGG 4275 0.0 18.33695 4 GGTATCA 16755 0.0 18.095362 1 GTGTAAG 815 0.0 18.0757 1 CAACGCA 24500 0.0 17.374855 5 TCAACGC 24590 0.0 17.349903 4 CATGGGA 5930 0.0 17.305267 4 ATCAACG 24715 0.0 17.262154 3 TATCAAC 24730 0.0 17.21326 2 GAGTACA 8290 0.0 17.082552 1 AACGCAG 25020 0.0 17.047337 6 >>END_MODULE