FastQCFastQC Report
Thu 26 May 2016
SRR937717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087171
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105400.5049897684473386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81140.3887558805675242No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT72020.3450603711914357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55620.2664851131028555No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT49460.23697147957690098No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40410.19361135239997104No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC32100.15379669418557465No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31190.1494367255965132No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23370.11196974277622676No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21860.10473506962294894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93800.024.9462131
TACATGG92500.024.5155432
TAAGCGT2151.6516424E-924.319024
ACATGGG94550.022.8756283
GTATCAA177950.022.3969251
GAGTACT86850.022.28730612-13
AGTACTT93500.020.9815812-13
CATGGGG39250.020.9506874
AGAGTAC136800.020.90307210-11
GAGTACA75000.020.7995971
GTACTTT94200.020.49790214-15
AGTACAT71450.020.2937582
TATCAAC205450.019.3911132
TCAACGC204000.019.3858574
ATCAACG204550.019.3816033
CAACGCA204650.019.3242845
GGTATCA138500.019.2300591
AACGCAG207650.019.035956
CATGGGA54600.018.9782284
TACTTTT108700.017.91653614-15
Produced by FastQC (version 0.11.5)