##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937717_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2087171 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.563613139507975 31.0 31.0 34.0 30.0 34.0 2 31.695205615639544 33.0 31.0 34.0 30.0 34.0 3 31.712997641304906 33.0 31.0 34.0 30.0 34.0 4 35.33033709264837 37.0 35.0 37.0 33.0 37.0 5 35.322315708679355 37.0 35.0 37.0 33.0 37.0 6 35.373294761186315 37.0 35.0 37.0 33.0 37.0 7 35.308915273353264 37.0 35.0 37.0 33.0 37.0 8 35.296575604011366 37.0 35.0 37.0 33.0 37.0 9 36.89078566154858 39.0 37.0 39.0 33.0 39.0 10-11 36.76960584446603 39.0 37.0 39.0 33.0 39.0 12-13 36.64093502640655 39.0 37.0 39.0 32.0 39.0 14-15 37.733335936538026 40.0 37.5 41.0 32.5 41.0 16-17 37.67046710595346 40.0 37.0 41.0 32.0 41.0 18-19 37.62621150830478 40.0 37.0 41.0 32.0 41.0 20-21 37.53741499857942 40.0 37.0 41.0 32.0 41.0 22-23 37.334105830332064 40.0 37.0 41.0 32.0 41.0 24-25 37.155791978711854 39.0 36.5 41.0 31.0 41.0 26-27 37.098052579304714 39.0 36.5 41.0 31.0 41.0 28-29 37.1468806341215 39.0 37.0 41.0 31.0 41.0 30-31 36.96735677143847 39.0 36.0 41.0 31.0 41.0 32-33 36.721617442940705 39.0 36.0 41.0 30.0 41.0 34-35 36.71459022763348 39.0 36.0 41.0 30.0 41.0 36-37 36.613867526906034 39.0 35.5 41.0 30.0 41.0 38-39 36.47339820263888 39.0 35.0 41.0 30.0 41.0 40-41 36.24602224733862 39.0 35.0 41.0 30.0 41.0 42-43 35.97698295923046 39.0 35.0 40.5 29.0 41.0 44-45 35.717387794291895 39.0 35.0 40.0 27.5 41.0 46-47 35.348254886638415 39.0 35.0 40.0 26.5 41.0 48-49 34.95527510683121 38.0 34.5 40.0 24.5 41.0 50-51 34.004152271184296 37.5 33.5 39.5 23.5 40.5 52-53 34.11673312823913 38.0 33.5 39.5 23.5 40.5 54-55 34.47423378343221 38.0 34.0 40.0 23.5 41.0 56-57 34.25371663366346 38.0 34.0 40.0 23.0 41.0 58-59 33.998336743850885 38.0 33.5 40.0 22.0 41.0 60-61 33.65420490223369 38.0 33.0 40.0 20.5 41.0 62-63 33.29315686160837 37.0 33.0 40.0 19.5 41.0 64-65 32.91063717347548 36.5 32.0 39.5 18.0 41.0 66-67 32.477878429702216 36.0 31.5 39.0 15.5 41.0 68-69 31.970573565845825 35.5 31.0 39.0 11.5 40.0 70-71 31.49797045857766 35.0 31.0 38.0 8.0 40.0 72-73 31.03832077007586 35.0 30.0 37.5 7.0 40.0 74-75 30.57764672851434 35.0 30.0 37.0 3.5 39.0 76-77 30.071540137343803 34.0 29.0 36.5 2.0 39.0 78-79 29.526267133838097 34.0 29.0 36.0 2.0 38.5 80-81 28.974535387852747 34.0 28.5 35.5 2.0 37.0 82-83 28.427946248774056 34.0 27.5 35.0 2.0 37.0 84-85 27.94225269515531 33.5 26.5 35.0 2.0 36.0 86-87 27.463810104682366 33.0 26.0 35.0 2.0 36.0 88-89 26.94153904974724 33.0 25.5 35.0 2.0 35.5 90-91 26.395966837408146 32.5 24.5 35.0 2.0 35.0 92-93 25.78439092915722 32.0 23.5 34.5 2.0 35.0 94-95 25.104668472300546 32.0 19.5 34.0 2.0 35.0 96-97 24.342287958197964 31.0 12.0 34.0 2.0 35.0 98-99 23.365073106132655 31.0 2.0 34.0 2.0 35.0 100-101 20.99878998893718 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13806790900776278 1101 2 0.24863369112227574 1101 3 0.310734618149052 1101 4 0.26561173934063476 1101 5 0.29317365816421415 1101 6 0.2957425126030415 1101 7 0.2892622092025121 1101 8 0.24347717252839374 1101 9 0.31726138753115407 1101 10-11 0.2676308187639478 1101 12-13 0.399878546138126 1101 14-15 0.5155052724984088 1101 16-17 0.577244404109166 1101 18-19 0.48574163737875153 1101 20-21 0.5919313930903698 1101 22-23 0.49753967050467907 1101 24-25 0.4297311621761537 1101 26-27 0.32901035672035306 1101 28-29 0.29366548307302054 1101 30-31 0.4370044466226446 1101 32-33 0.2845981763377665 1101 34-35 0.36591500475644523 1101 36-37 0.4088706033401692 1101 38-39 0.44082459675600205 1101 40-41 0.5575766224184306 1101 42-43 0.39336560750505356 1101 44-45 0.4908173321086906 1101 46-47 0.5741670907806622 1101 48-49 0.633289141989998 1101 50-51 0.5252705737401158 1101 52-53 0.4760302066582156 1101 54-55 0.545136096912465 1101 56-57 0.4644313880283235 1101 58-59 0.5361062152121789 1101 60-61 0.6030798003379019 1101 62-63 0.6849549486364168 1101 64-65 0.5209727003570492 1101 66-67 0.7060339314272284 1101 68-69 0.6105517580480821 1101 70-71 0.7566938649546415 1101 72-73 0.6668129815461334 1101 74-75 0.6925433721055754 1101 76-77 0.8298638045965774 1101 78-79 0.9426444135830465 1101 80-81 0.8896568865505721 1101 82-83 0.9118987510672021 1101 84-85 1.1786905545952457 1101 86-87 1.2404875892765617 1101 88-89 1.2268996667631242 1101 90-91 1.2775292030183572 1101 92-93 1.3052114032059983 1101 94-95 1.5693376064017528 1101 96-97 1.8628759028809583 1101 98-99 2.105370501596827 1101 100-101 2.6600158312702433 1102 1 0.21594751801818646 1102 2 0.18208498632025183 1102 3 0.2073879532189835 1102 4 0.20532666130473132 1102 5 0.12589081547388048 1102 6 0.18858591240375233 1102 7 0.0968090153903276 1102 8 0.15920983324613758 1102 9 0.2698419886283929 1102 10-11 0.2686776508948938 1102 12-13 0.2995787705198083 1102 14-15 0.4687589127443701 1102 16-17 0.40965479703653784 1102 18-19 0.4271076018864193 1102 20-21 0.5157386175662921 1102 22-23 0.6294555554357402 1102 24-25 0.6656743752921628 1102 26-27 0.47513132798912494 1102 28-29 0.3944966165340631 1102 30-31 0.5896851141131307 1102 32-33 0.5570677408001146 1102 34-35 0.6337751617071348 1102 36-37 0.7376770177548124 1102 38-39 0.7126370056241029 1102 40-41 0.6520458628562622 1102 42-43 0.6407203073958812 1102 44-45 0.6711937299938455 1102 46-47 0.5980472737168512 1102 48-49 0.6972352734444129 1102 50-51 0.6625493820076116 1102 52-53 0.48141526108056354 1102 54-55 0.504530107541747 1102 56-57 0.5231819029202214 1102 58-59 0.44717758214028436 1102 60-61 0.6192056319743457 1102 62-63 0.8000894346922465 1102 64-65 0.7133744314527064 1102 66-67 0.724151984357583 1102 68-69 0.721343892088349 1102 70-71 0.8936166698209433 1102 72-73 0.8913724511624856 1102 74-75 0.9816921512426298 1102 76-77 1.0483710046118233 1102 78-79 0.931147896846543 1102 80-81 0.959801522197715 1102 82-83 0.9717112404119881 1102 84-85 1.2647324045846027 1102 86-87 1.1980902526309762 1102 88-89 1.2310107542253874 1102 90-91 1.2273446890604198 1102 92-93 1.484860651966816 1102 94-95 1.7659971890031194 1102 96-97 1.9123778475574795 1102 98-99 2.0995573324359107 1102 100-101 2.4976703385464987 1103 1 0.30335029405920366 1103 2 0.30221336779663943 1103 3 0.31851962759687424 1103 4 0.26527476684430695 1103 5 0.23702207302879685 1103 6 0.2973675388470056 1103 7 0.3259407731328139 1103 8 0.3038309411616069 1103 9 0.41295002914358747 1103 10-11 0.3807816690683765 1103 12-13 0.35367447307493194 1103 14-15 0.5804375630715768 1103 16-17 0.4391633627486726 1103 18-19 0.4211442197848001 1103 20-21 0.3849945885554149 1103 22-23 0.4997782806243478 1103 24-25 0.5331040364150752 1103 26-27 0.43973888915788706 1103 28-29 0.33911845388973205 1103 30-31 0.2559924793751094 1103 32-33 0.15958295483871154 1103 34-35 0.36962537637096915 1103 36-37 0.495772240963511 1103 38-39 0.5187308533169883 1103 40-41 0.5701422323281591 1103 42-43 0.5180622272825062 1103 44-45 0.2756477961019712 1103 46-47 0.5288799818157273 1103 48-49 0.5465370949608186 1103 50-51 0.48626994388498446 1103 52-53 0.4165577103323628 1103 54-55 0.6034412617245621 1103 56-57 0.514679131047437 1103 58-59 0.614249192327442 1103 60-61 0.7157776798256208 1103 62-63 0.8016109427577689 1103 64-65 0.8900063976066832 1103 66-67 0.9341601124055998 1103 68-69 0.8536999839561261 1103 70-71 1.0059837032918253 1103 72-73 1.0953711381701048 1103 74-75 0.9440681464074849 1103 76-77 0.8793310521296434 1103 78-79 0.9948436577568849 1103 80-81 0.7986338968382043 1103 82-83 1.0465659776050984 1103 84-85 1.1522702312696111 1103 86-87 1.2308597286844964 1103 88-89 1.3247922764628939 1103 90-91 1.3259571895814481 1103 92-93 1.5167178067331868 1103 94-95 1.467178777932304 1103 96-97 1.8029961002355641 1103 98-99 1.8222810621678924 1103 100-101 2.1531367634558407 1104 1 0.0964234165888449 1104 2 0.20529307794156537 1104 3 0.19249525342032925 1104 4 0.17254914102085195 1104 5 0.09867391756107224 1104 6 0.10743670880747658 1104 7 0.0980586914463828 1104 8 0.0978180953118013 1104 9 0.2741473282213178 1104 10-11 0.2660789352080357 1104 12-13 0.25901538216584186 1104 14-15 0.47944283316380876 1104 16-17 0.541562901615606 1104 18-19 0.4546932975186593 1104 20-21 0.47764280989928665 1104 22-23 0.4587168511118378 1104 24-25 0.4830447478775284 1104 26-27 0.578420542649333 1104 28-29 0.5162437832705038 1104 30-31 0.5158187310220157 1104 32-33 0.49806945022738347 1104 34-35 0.43453562011273306 1104 36-37 0.4908438812797158 1104 38-39 0.6014021313143587 1104 40-41 0.6985019161226163 1104 42-43 0.6661567595618223 1104 44-45 0.6764276050611286 1104 46-47 0.7353580779949098 1104 48-49 0.7362686496380206 1104 50-51 0.6359595025147584 1104 52-53 0.6536945680398745 1104 54-55 0.6726227505256119 1104 56-57 0.7880783405335947 1104 58-59 0.7679621369914713 1104 60-61 0.7375999789033543 1104 62-63 0.8366521153532887 1104 64-65 0.5919365688846838 1104 66-67 0.7423271084530256 1104 68-69 0.8299788047729848 1104 70-71 0.7022337032918244 1104 72-73 0.6586427126101633 1104 74-75 0.9760221977908863 1104 76-77 1.015035926964952 1104 78-79 0.9092664179676895 1104 80-81 1.0420536926221295 1104 82-83 1.034970951254639 1104 84-85 1.1744803761971454 1104 86-87 1.2327133189348274 1104 88-89 1.2832161895063692 1104 90-91 1.3079030064457449 1104 92-93 1.3452800596976147 1104 94-95 1.4573393510548307 1104 96-97 1.6547673452948501 1104 98-99 2.081531720929423 1104 100-101 2.6539371587127576 1105 1 0.38642141982462874 1105 2 0.1976443616732304 1105 3 0.2914447335789738 1105 4 0.3098367074943269 1105 5 0.19041350025441517 1105 6 0.23785741166848595 1105 7 0.29659550699997794 1105 8 0.255284073521473 1105 9 0.235293411150181 1105 10-11 0.38415543261053386 1105 12-13 0.4674615668102149 1105 14-15 0.5090892352336098 1105 16-17 0.5214431554947865 1105 18-19 0.4810017327381644 1105 20-21 0.6417256295351663 1105 22-23 0.6468048939074365 1105 24-25 0.5998656944461729 1105 26-27 0.5675297511456492 1105 28-29 0.5447743653358046 1105 30-31 0.5284182919045506 1105 32-33 0.7005850744807276 1105 34-35 0.7273867988862719 1105 36-37 0.8026434138320937 1105 38-39 0.8007056531285741 1105 40-41 0.8654083651590483 1105 42-43 0.8923297732080826 1105 44-45 0.8652627278024028 1105 46-47 0.925631041636727 1105 48-49 0.8805926766847918 1105 50-51 0.8260532693677902 1105 52-53 0.9831462642654785 1105 54-55 0.9876581717575377 1105 56-57 0.9305858667987295 1105 58-59 0.9247084480975758 1105 60-61 0.8407988762457776 1105 62-63 0.9784208815236681 1105 64-65 1.0559943863019328 1105 66-67 1.1027116903613248 1105 68-69 1.0991945670931997 1105 70-71 1.408825201172185 1105 72-73 1.390226295965686 1105 74-75 1.3358663093844036 1105 76-77 1.5562917209944587 1105 78-79 1.5046764415534 1105 80-81 1.6327566005593503 1105 82-83 1.476608370445419 1105 84-85 1.5925622708362646 1105 86-87 1.6681454093250991 1105 88-89 1.6314985882857869 1105 90-91 1.6591825781824845 1105 92-93 2.1014540290600827 1105 94-95 2.1791347364815223 1105 96-97 2.2864664252473403 1105 98-99 2.478501033906003 1105 100-101 2.3737514596404807 1106 1 0.15688233578796584 1106 2 0.0965501785268934 1106 3 0.10397417305141943 1106 4 0.04856090693372295 1106 5 0.19904393095229267 1106 6 0.059145978986101966 1106 7 0.16285085758387652 1106 8 -0.08713527841116786 1106 9 0.14016562775262287 1106 10-11 0.0588926973843229 1106 12-13 -0.044403023199286906 1106 14-15 -0.05326710657570999 1106 16-17 -0.060300124583662296 1106 18-19 0.11930740905454229 1106 20-21 0.1866538036572507 1106 22-23 0.2987164326362759 1106 24-25 0.17482534789048998 1106 26-27 0.19001211320161104 1106 28-29 0.38299947723796635 1106 30-31 0.4319288926885676 1106 32-33 0.39826775364646494 1106 34-35 0.35266188903862883 1106 36-37 0.3490323005760345 1106 38-39 0.32057263175713047 1106 40-41 0.4463717405248744 1106 42-43 0.5201337623048587 1106 44-45 0.6310017454313339 1106 46-47 0.6572344279160731 1106 48-49 0.6402777805047819 1106 50-51 0.4408948817886085 1106 52-53 0.5469066919518397 1106 54-55 0.599699334253124 1106 56-57 0.6488420719316892 1106 58-59 0.5443311595405547 1106 60-61 0.6214011274162843 1106 62-63 0.9120890848342711 1106 64-65 0.8712123091814448 1106 66-67 0.9050418809103178 1106 68-69 0.6732404707916899 1106 70-71 0.837433032650992 1106 72-73 0.8764454054329001 1106 74-75 1.0078597509777794 1106 76-77 1.02106602480724 1106 78-79 1.1166146463311541 1106 80-81 1.1471771159142072 1106 82-83 0.8607140153596937 1106 84-85 0.8973969078716983 1106 86-87 1.004869912489763 1106 88-89 1.0062440896770006 1106 90-91 1.269604730565053 1106 92-93 1.2011415523864422 1106 94-95 1.605508385483219 1106 96-97 1.68954975150233 1106 98-99 1.8778225425454416 1106 100-101 2.284440787300845 1107 1 0.20170444349999528 1107 2 0.18110278857212947 1107 3 0.22451901834494947 1107 4 0.2388230065414234 1107 5 0.16093738701112414 1107 6 0.10036941012077705 1107 7 0.16301375280990982 1107 8 0.1423467599526802 1107 9 0.21254770528266675 1107 10-11 0.28314306425528457 1107 12-13 0.23452699059092197 1107 14-15 0.5027147509387575 1107 16-17 0.4805768272162325 1107 18-19 0.45579139764284093 1107 20-21 0.5056882653907806 1107 22-23 0.5287193790185327 1107 24-25 0.5083272837278443 1107 26-27 0.4919343719900411 1107 28-29 0.3523848057762464 1107 30-31 0.4115814954332464 1107 32-33 0.3267994351032968 1107 34-35 0.44641320438785215 1107 36-37 0.30081543257323773 1107 38-39 0.49406365718138545 1107 40-41 0.45852075184597396 1107 42-43 0.4080100056888796 1107 44-45 0.3508439534630412 1107 46-47 0.3311595631726121 1107 48-49 0.5580301973586543 1107 50-51 0.40000770444897427 1107 52-53 0.45991588518766946 1107 54-55 0.39083638335736026 1107 56-57 0.5129519018381572 1107 58-59 0.5298967400884393 1107 60-61 0.5290924454812185 1107 62-63 0.6166737174651118 1107 64-65 0.5542499460639689 1107 66-67 0.6242012369105794 1107 68-69 0.7205786557869303 1107 70-71 0.7903290838566903 1107 72-73 0.6738713992780774 1107 74-75 0.5607175872012533 1107 76-77 0.6273482634965433 1107 78-79 0.9942724840765713 1107 80-81 0.9453984427279316 1107 82-83 1.1326898298179877 1107 84-85 1.1969423231796164 1107 86-87 1.239698124611209 1107 88-89 1.441552688143119 1107 90-91 1.4228255258885092 1107 92-93 1.4086119274521032 1107 94-95 1.538775561952491 1107 96-97 1.6674700764747428 1107 98-99 1.740589735304237 1107 100-101 2.11177921961146 1108 1 0.11262215963438393 1108 2 0.09368256210943571 1108 3 0.11043690463263545 1108 4 0.10380772677537209 1108 5 0.10873685114167841 1108 6 0.14020252376730014 1108 7 0.03356679639864524 1108 8 0.14357458614222196 1108 9 0.3237837215175645 1108 10-11 0.21311148380340939 1108 12-13 0.17338149590992202 1108 14-15 0.27810774833654506 1108 16-17 0.37074888622993285 1108 18-19 0.3627394373634303 1108 20-21 0.41467145197635347 1108 22-23 0.4277242091034523 1108 24-25 0.47870722469598803 1108 26-27 0.5104842575335908 1108 28-29 0.44217855729343825 1108 30-31 0.3684526258636893 1108 32-33 0.3567964404052759 1108 34-35 0.243620421610089 1108 36-37 0.38341226681443885 1108 38-39 0.4038353341442189 1108 40-41 0.38509268471936053 1108 42-43 0.6126507667051655 1108 44-45 0.7154011351799596 1108 46-47 0.7361473234787965 1108 48-49 0.6860243849220353 1108 50-51 0.699462533286102 1108 52-53 0.6900271200695514 1108 54-55 0.7062407016211623 1108 56-57 0.80722113880271 1108 58-59 0.7945313982731577 1108 60-61 0.8256398082185541 1108 62-63 0.8685314511593205 1108 64-65 0.777295281708362 1108 66-67 0.6735590783685481 1108 68-69 0.9204578469462135 1108 70-71 1.0604979213013053 1108 72-73 0.9636574371791617 1108 74-75 0.8125569443394092 1108 76-77 0.8304997294239129 1108 78-79 0.803902253189893 1108 80-81 0.9433980071354888 1108 82-83 0.9980567143564976 1108 84-85 0.9753981933549163 1108 86-87 1.1659103534152138 1108 88-89 1.2529320868894303 1108 90-91 1.5171732602406465 1108 92-93 1.7432645538249574 1108 94-95 1.7907149692291568 1108 96-97 2.029063528068395 1108 98-99 2.2008183305866815 1108 100-101 2.6549700249810435 1109 1 0.06922736858984635 1109 2 0.12732117805059318 1109 3 0.12767064907180625 1109 4 0.10999293559457612 1109 5 0.1331300139618392 1109 6 0.04428307659148345 1109 7 0.11172724349043506 1109 8 0.14900517995587137 1109 9 0.14940361628476495 1109 10-11 0.17011988834789804 1109 12-13 0.32880754795405664 1109 14-15 0.38243139937423365 1109 16-17 0.35135223760165957 1109 18-19 0.44485474380797285 1109 20-21 0.3871446952766746 1109 22-23 0.5271495617150848 1109 24-25 0.6234845303820649 1109 26-27 0.48099863564044654 1109 28-29 0.494935524935606 1109 30-31 0.5494646229478732 1109 32-33 0.4363417865675956 1109 34-35 0.5984606989305377 1109 36-37 0.23916260120221722 1109 38-39 0.29638331226198034 1109 40-41 0.4594417923751948 1109 42-43 0.4584564425545423 1109 44-45 0.5909828790831995 1109 46-47 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-0.6942384845804668 2316 96-97 -0.734544757782821 2316 98-99 -0.7294911543796054 2316 100-101 -0.6401267707627518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8845.0 3 1633.0 4 1639.0 5 1987.0 6 2132.0 7 2599.0 8 3183.0 9 4064.0 10 5196.0 11 6824.0 12 8680.0 13 11283.0 14 12693.0 15 15474.0 16 19141.0 17 24849.0 18 21469.0 19 15897.0 20 15531.0 21 16786.0 22 18172.0 23 20147.0 24 22559.0 25 25652.0 26 29811.0 27 34489.0 28 40619.0 29 48639.0 30 58912.0 31 72306.0 32 89184.0 33 114973.0 34 151243.0 35 205590.0 36 286333.0 37 368204.0 38 268699.0 39 31727.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16234919456654 19.38742248790624 15.078860652750627 22.3713676647766 2 14.799716795987846 20.52479260785617 40.832183357774824 23.84330723838116 3 19.394223375624758 24.790032679738562 30.28676470588235 25.528979238754324 4 12.225207204254938 16.56932507319239 38.192805066792104 33.012662655760565 5 13.00353109007331 38.04361561713355 34.492825626421336 14.460027666371808 6 31.01239410457649 36.58241774601042 17.596372579010076 14.80881557040302 7 27.462275629667833 33.65360514662879 20.94020664494388 17.943912578759498 8 25.782626590166124 34.67459014307113 20.008010561646987 19.53477270511576 9 25.803427567013333 17.20338962701136 21.281411560174522 35.71177124580079 10-11 24.541485534042685 26.910224812560152 27.341701452454725 21.206588200942434 12-13 25.60350422505181 24.506284934142112 28.011014211723438 21.879196629082635 14-15 22.51230043900298 25.65677573392059 27.239721942369695 24.591201884706734 16-17 21.186833587399168 29.159882840796165 27.635175708793252 22.018107863011416 18-19 21.01897782878656 27.783070739472638 30.265339051012518 20.932612380728283 20-21 22.03676162559702 26.335132643617733 30.307131102380637 21.320974628404617 22-23 21.609805348686255 26.331266347368864 30.402217288938903 21.65671101500597 24-25 21.71058759139857 26.364533273161108 30.451099663451615 21.473779471988706 26-27 21.33788508000089 26.485033759961546 30.717063359919866 21.460017800117694 28-29 21.029489985699858 27.31557202359583 30.368765901551164 21.286172089153148 30-31 21.30365900276423 26.543377662179996 30.309616792071903 21.84334654298387 32-33 21.1684316691881 26.64639915471988 30.386547559013522 21.7986216170785 34-35 21.8241470162888 26.644157182533558 30.16546199627686 21.366233804900784 36-37 21.538056389367412 26.29983426188926 30.48464953975506 21.677459808988267 38-39 21.287335020140866 26.92447295266536 30.258878545056483 21.529313482137287 40-41 21.526710081065335 26.86015671527322 30.091560069027118 21.521573134634327 42-43 21.23567909652265 26.86543872621986 29.7011696804986 22.19771249675889 44-45 21.41962508921903 26.816485011563362 29.682273424481387 22.081616474736222 46-47 21.876636289451906 27.18697100059948 29.28559887623752 21.6507938337111 48-49 21.804774134311184 27.181846034456598 29.059426005144097 21.953953826088128 50-51 21.498236093764746 27.47556213630165 29.247921097644113 21.77828067228949 52-53 21.82951403983658 27.79380858938732 28.459256732900844 21.917420637875253 54-55 21.513370285873982 27.413803949212745 28.811120912407308 22.261704852505964 56-57 21.87319917234831 27.522106753243197 28.59281692226569 22.0118771521428 58-59 22.238212110724483 27.219825072326508 28.618809645070876 21.923153171878127 60-61 21.821246329661616 27.379758238323987 29.197951403315614 21.601044028698787 62-63 21.50062281298303 27.541608684286267 29.27725127869303 21.680517224037676 64-65 21.40282442781071 27.86848298973254 28.886828473542653 21.8418641089141 66-67 21.547826650520218 27.483830548711186 28.562314046809494 22.406028753959102 68-69 21.4639660346207 27.47783246265083 28.888551759300356 22.169649743428113 70-71 21.8473610636394 27.393702306933086 28.658348662961952 22.100587966465557 72-73 21.73194825095665 27.274246022544794 28.894224006782355 22.099581719716205 74-75 21.460988825419655 28.110143776735953 28.44451944359697 21.984347954247426 76-77 21.628254546371245 27.535842175583745 28.41430109708329 22.421602180961724 78-79 21.688955903326338 27.61242658957872 28.463555297315207 22.235062209779734 80-81 21.83729180255612 27.6348309441197 28.31367602642399 22.214201226900194 82-83 21.747027475491333 27.50557127036968 28.722044321937446 22.025356932201547 84-85 21.70018526404327 27.65775168473139 28.430253296610182 22.211809754615157 86-87 21.542827459196626 27.843849679967892 28.35484004155712 22.258482819278356 88-89 21.659547406637586 27.629018732860537 28.097250220323755 22.614183640178123 90-91 21.675403228226518 27.305966109263235 28.39660588484544 22.622024777664812 92-93 21.94464776076909 27.41003862783446 28.392862672779202 22.252450938617248 94-95 21.663586324673886 27.35811802346949 28.41485256968699 22.56344308216963 96-97 21.30628766632711 27.348093749009887 28.755687453374644 22.589931131288363 98-99 21.433667441082243 27.487840043094568 28.21647723971979 22.8620152761034 100-101 21.56530763898623 26.819522877787357 28.778689642062332 22.83647984116408 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3034.0 1 1764.0 2 520.5 3 529.0 4 442.0 5 440.5 6 748.0 7 1573.0 8 2653.0 9 3190.5 10 3054.5 11 2865.5 12 3060.0 13 3317.5 14 3468.0 15 3567.0 16 3707.5 17 3849.5 18 4007.0 19 4299.0 20 4766.5 21 5481.5 22 6479.0 23 7918.5 24 9514.5 25 11642.0 26 14836.5 27 18322.0 28 20802.5 29 23331.0 30 26822.5 31 30903.5 32 34955.5 33 38674.5 34 43068.5 35 46927.0 36 51266.0 37 55997.5 38 58871.0 39 61093.5 40 63003.0 41 65563.5 42 68032.5 43 71234.5 44 75178.5 45 76972.0 46 78505.0 47 84898.5 48 95589.0 49 93382.5 50 80326.5 51 71928.0 52 66709.0 53 63409.5 54 61282.5 55 56840.5 56 50291.0 57 44771.5 58 40657.5 59 36028.5 60 29637.5 61 23489.0 62 18428.5 63 13781.0 64 10245.0 65 7826.0 66 5667.5 67 3936.5 68 2738.5 69 1916.5 70 1363.0 71 926.0 72 665.0 73 509.0 74 372.0 75 255.0 76 166.5 77 106.5 78 73.5 79 49.5 80 34.5 81 25.0 82 21.0 83 19.0 84 12.5 85 12.0 86 11.5 87 7.0 88 4.5 89 5.0 90 4.0 91 3.0 92 3.0 93 2.0 94 1.5 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34367093065206444 2 0.32062538239559674 3 0.30524571297703923 4 0.2880453973344781 5 0.28478739882836623 6 0.23045548256467727 7 0.21871710559412716 8 0.2357257742657406 9 0.2364923621495316 10-11 0.2349112746392126 12-13 0.22856296872656817 14-15 0.22638298443203742 16-17 0.2254487054486671 18-19 0.22714957231582847 20-21 0.212177152710535 22-23 0.20443940625851934 24-25 0.2041519358020977 26-27 0.2204179724612885 28-29 0.24068463963901376 30-31 0.23261151098783953 32-33 0.24056486028217142 34-35 0.22947329183857001 36-37 0.20923058053221325 38-39 0.20798487522105277 40-41 0.20225942196398858 42-43 0.21804634119581 44-45 0.2163454743286487 46-47 0.21677668001328113 48-49 0.2291618655107799 50-51 0.23967849304153804 52-53 0.25948999866326233 54-55 0.22422695600887518 56-57 0.18817336959932848 58-59 0.18733491410143202 60-61 0.19579133669450183 62-63 0.20518203827094186 64-65 0.19107203003491327 66-67 0.1990014234578767 68-69 0.20249898067767327 70-71 0.19447376376923597 72-73 0.19730055659071538 74-75 0.2004866874827218 76-77 0.22056170768949934 78-79 0.22523310260635088 80-81 0.21284791710885215 82-83 0.21021277125832047 84-85 0.20113349600967048 86-87 0.20386446534567607 88-89 0.2127520936233782 90-91 0.21313538756527378 92-93 0.20824838980610597 94-95 0.2079130076069474 96-97 0.19533617513850088 98-99 0.20649961119620766 100-101 0.19236564708881065 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2087171.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.89299492530075 #Duplication Level Percentage of deduplicated Percentage of total 1 73.28395516564356 38.02923913516709 2 14.315386569068888 14.857365651648205 3 5.19207825990481 8.08297472379014 4 2.430294403650337 5.04461020622455 5 1.3361830030338415 3.466926889785413 6 0.7910284978604503 2.4629302695144375 7 0.5262357758487409 1.9115565311941294 8 0.3641849925254904 1.5118919975196774 9 0.256229480415798 1.196686361423651 >10 1.3715104368798454 12.82185140487904 >50 0.08826504954698848 3.118446215206904 >100 0.04123684278425951 3.7102915236911747 >500 0.001383049798727268 0.48521707728684066 >1k 0.0016596597584727201 1.7967529520042824 >5k 2.7660995974545334E-4 0.9989510047918994 >10k+ 9.220331991515112E-5 0.504308055872527 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10540 0.5049897684473386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8114 0.3887558805675242 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7202 0.3450603711914357 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5562 0.2664851131028555 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4946 0.23697147957690098 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4041 0.19361135239997104 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3210 0.15379669418557465 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3119 0.1494367255965132 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2337 0.11196974277622676 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2186 0.10473506962294894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.791174273693914E-5 0.0 0.0 0.0 0.0 7 9.582348547387828E-5 0.0 0.0 0.0 0.0 8 9.582348547387828E-5 0.0 0.0 0.0 0.0 9 9.582348547387828E-5 0.0 0.0 0.0 0.0 10-11 9.582348547387828E-5 0.0 0.0 2.395587136846957E-5 0.0 12-13 1.6769109957928699E-4 0.0 0.0 4.791174273693914E-5 4.791174273693914E-5 14-15 2.874704564216348E-4 0.0 0.0 9.582348547387828E-5 4.791174273693914E-5 16-17 3.3538219915857397E-4 0.0 0.0 9.582348547387828E-5 4.791174273693914E-5 18-19 4.5516155600092183E-4 0.0 0.0 1.1977935684234785E-4 4.791174273693914E-5 20-21 6.228526555802088E-4 0.0 0.0 1.6769109957928699E-4 4.791174273693914E-5 22-23 6.947202696856175E-4 0.0 0.0 2.874704564216348E-4 4.791174273693914E-5 24-25 8.384554978964349E-4 0.0 0.0 3.3538219915857397E-4 4.791174273693914E-5 26-27 8.624113692649045E-4 0.0 0.0 7.186761410540872E-4 4.791174273693914E-5 28-29 0.0011259259543180697 0.0 0.0 0.0014852640248451134 4.791174273693914E-5 30-31 0.0012457053111604177 0.0 0.0 0.003234042634743392 4.791174273693914E-5 32-33 0.0012457053111604177 0.0 0.0 0.005869188485275045 7.18676141054087E-5 34-35 0.0012457053111604177 0.0 0.0 0.008528290207175167 9.582348547387828E-5 36-37 0.0013415287966342959 0.0 0.0 0.011474862385496925 9.582348547387828E-5 38-39 0.0015810875103189916 0.0 0.0 0.014828684377082664 9.582348547387828E-5 40-41 0.0015810875103189916 0.0 0.0 0.019643814522145045 9.582348547387828E-5 42-43 0.0018446020953721569 0.0 0.0 0.025800473463841726 9.582348547387828E-5 44-45 0.0021560284231622612 0.0 0.0 0.03166966194911677 9.582348547387828E-5 46-47 0.0022518519086361394 0.0 0.0 0.039000158587868455 9.582348547387828E-5 48-49 0.0023476753941100176 0.0 0.0 0.045683846699671474 9.582348547387828E-5 50-51 0.0023476753941100176 0.0 0.0 0.05543388634663858 9.582348547387828E-5 52-53 0.0023476753941100176 0.0 0.0 0.08030008082710999 9.582348547387828E-5 54-55 0.0023476753941100176 0.0 0.0 0.11735981383413241 9.582348547387828E-5 56-57 0.0024434988795838963 0.0 0.0 0.14677762387461307 9.582348547387828E-5 58-59 0.0024434988795838963 0.0 0.0 0.18879622225490866 9.582348547387828E-5 60-61 0.0024434988795838963 0.0 0.0 0.24140331578006785 9.582348547387828E-5 62-63 0.0024434988795838963 0.0 0.0 0.2756602118369793 9.582348547387828E-5 64-65 0.0024434988795838963 0.0 0.0 0.30270639061198146 9.582348547387828E-5 66-67 0.0024914106223208354 0.0 0.0 0.3828866920822491 9.582348547387828E-5 68-69 0.0024914106223208354 0.0 0.0 0.46457621344873035 9.582348547387828E-5 70-71 0.0025393223650577745 0.0 0.0 0.5049658125759701 9.582348547387828E-5 72-73 0.002611189979163183 0.0 0.0 0.587254230726663 9.582348547387828E-5 74-75 0.0026351458505316527 0.0 0.0 0.7075127049963803 9.582348547387828E-5 76-77 0.0026351458505316527 0.0 0.0 0.8193147566730277 9.582348547387828E-5 78-79 0.0026351458505316527 0.0 0.0 0.939932569013272 9.582348547387828E-5 80-81 0.002874704564216348 0.0 0.0 1.098592305086646 9.582348547387828E-5 82-83 0.003018439792427166 0.0 0.0 1.2657324196244581 9.582348547387828E-5 84-85 0.003114263277901044 0.0 0.0 1.4201759223369814 9.582348547387828E-5 86-87 0.003234042634743392 0.0 0.0 1.6481160384079696 9.582348547387828E-5 88-89 0.0032579985061118614 0.0 0.0 1.9252854701411624 9.582348547387828E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9380 0.0 24.946213 1 TACATGG 9250 0.0 24.515543 2 TAAGCGT 215 1.6516424E-9 24.31902 4 ACATGGG 9455 0.0 22.875628 3 GTATCAA 17795 0.0 22.396925 1 GAGTACT 8685 0.0 22.287306 12-13 AGTACTT 9350 0.0 20.98158 12-13 CATGGGG 3925 0.0 20.950687 4 AGAGTAC 13680 0.0 20.903072 10-11 GAGTACA 7500 0.0 20.799597 1 GTACTTT 9420 0.0 20.497902 14-15 AGTACAT 7145 0.0 20.293758 2 TATCAAC 20545 0.0 19.391113 2 TCAACGC 20400 0.0 19.385857 4 ATCAACG 20455 0.0 19.381603 3 CAACGCA 20465 0.0 19.324284 5 GGTATCA 13850 0.0 19.230059 1 AACGCAG 20765 0.0 19.03595 6 CATGGGA 5460 0.0 18.978228 4 TACTTTT 10870 0.0 17.916536 14-15 >>END_MODULE