FastQCFastQC Report
Thu 26 May 2016
SRR937717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087171
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115670.5541951282381751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96170.4607672299011437No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94480.45267014537860095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65980.31612167857832446No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34660.16606210032623106No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32570.15604854609421076No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25920.12418723717414624No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25090.12021056252698029No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25080.12016265078424336No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24390.11685674053539456No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22180.10626824539053102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTG250.00502065557.0034269
TTTACGC950.001226632225.0009023
GTACATG91350.023.09451
GAGTACT101550.022.66384112-13
TACATGG91500.022.6346972
ACATGGG91600.021.6246873
GTACTTT107450.021.3947214-15
AGTACTT107300.021.29438212-13
AGAGTAC157700.021.07054310-11
GTATCAA219300.020.6484951
ACGACGG1200.004763783419.7923815
GTATAGG5300.019.7233621
TACTTTT122050.018.6797514-15
CATGGGA53200.018.4828114
CATGGGG39750.018.403184
ACTTTTT126500.018.2854716-17
AGGACCG7800.018.269895
CAACGCA250500.017.9956595
TCAACGC250600.017.988484
ATCAACG251000.017.9787373
Produced by FastQC (version 0.11.5)