##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937717_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2087171 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16504589226278 33.0 31.0 34.0 31.0 34.0 2 32.36631593673925 34.0 31.0 34.0 31.0 34.0 3 32.43419537737924 34.0 31.0 34.0 31.0 34.0 4 36.06137302597631 37.0 35.0 37.0 35.0 37.0 5 35.97727881424186 37.0 35.0 37.0 35.0 37.0 6 36.02769490377166 37.0 35.0 37.0 35.0 37.0 7 35.97761850849787 37.0 35.0 37.0 35.0 37.0 8 35.94041551938006 37.0 35.0 37.0 35.0 37.0 9 37.615576778328176 39.0 37.0 39.0 35.0 39.0 10-11 37.53356720652021 39.0 37.0 39.0 35.0 39.0 12-13 37.429448761026286 39.0 37.0 39.0 34.5 39.0 14-15 38.605371337566496 40.0 38.0 41.0 34.0 41.0 16-17 38.57975077269663 40.0 38.0 41.0 34.0 41.0 18-19 38.57483622568539 40.0 38.0 41.0 34.0 41.0 20-21 38.469785657236514 40.0 38.0 41.0 34.0 41.0 22-23 38.41596879220725 40.0 38.0 41.0 34.0 41.0 24-25 38.43404158068505 40.0 38.0 41.0 34.0 41.0 26-27 38.248896233226695 40.0 38.0 41.0 34.0 41.0 28-29 38.21170929454271 40.0 38.0 41.0 33.5 41.0 30-31 38.22190467383842 40.0 38.0 41.0 34.0 41.0 32-33 38.084495472579874 40.0 37.0 41.0 33.5 41.0 34-35 37.92656423455481 40.0 37.0 41.0 33.0 41.0 36-37 37.74938948461818 40.0 37.0 41.0 33.0 41.0 38-39 37.6048637126522 40.0 37.0 41.0 33.0 41.0 40-41 37.44366633112476 40.0 37.0 41.0 33.0 41.0 42-43 37.195726416283094 40.0 36.0 41.0 32.0 41.0 44-45 36.861678319601026 39.5 36.0 41.0 31.0 41.0 46-47 36.49475078946574 39.0 36.0 41.0 30.5 41.0 48-49 36.118715237036156 39.0 35.5 41.0 29.5 41.0 50-51 35.85978939914362 39.0 35.0 40.0 29.0 41.0 52-53 35.60799761974462 39.0 35.0 40.0 28.0 41.0 54-55 35.38122008211114 38.5 35.0 40.0 27.5 41.0 56-57 35.095441868442975 38.0 34.5 40.0 27.0 41.0 58-59 34.84474535148294 38.0 34.0 40.0 26.0 41.0 60-61 34.474700683365185 38.0 34.0 40.0 26.0 41.0 62-63 34.07299497741201 37.0 33.5 40.0 25.5 41.0 64-65 33.67142989242376 37.0 33.0 39.5 24.0 41.0 66-67 33.24137289182343 36.0 32.5 39.0 22.5 40.0 68-69 32.76845859778619 36.0 32.0 39.0 22.0 40.0 70-71 32.32202224925509 35.0 31.5 38.0 21.0 40.0 72-73 31.83646644189671 35.0 31.0 37.5 19.5 39.5 74-75 31.362436762488556 35.0 31.0 37.0 18.0 39.0 76-77 30.10177244701081 33.5 29.5 35.5 18.0 38.0 78-79 30.71527991717018 34.0 30.5 36.0 18.0 38.0 80-81 30.54384834783542 34.0 30.5 35.5 16.0 37.0 82-83 30.155560325435722 34.0 30.0 35.0 10.0 37.0 84-85 29.735080403091075 34.0 30.0 35.0 7.0 36.5 86-87 29.412118125443484 34.0 29.5 35.0 4.0 36.0 88-89 28.984666565413185 34.0 29.0 35.0 2.0 36.0 90-91 28.46640189040572 34.0 29.0 35.0 2.0 35.0 92-93 28.001558808549945 33.0 27.5 35.0 2.0 35.0 94-95 27.481924097258922 33.0 27.0 35.0 2.0 35.0 96-97 26.935567330132507 33.0 26.0 35.0 2.0 35.0 98-99 26.10331400733337 32.5 24.5 34.0 2.0 35.0 100-101 23.65830710564683 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28895315658112253 1101 2 0.29288416810311446 1101 3 0.2747824479570298 1101 4 0.18151942297294 1101 5 0.13829240578348134 1101 6 0.207281352746854 1101 7 0.18888527994521098 1101 8 0.254183910818675 1101 9 0.2705276150968743 1101 10-11 0.2869444782932362 1101 12-13 0.276564537409854 1101 14-15 0.3947378174384184 1101 16-17 0.48374770642345055 1101 18-19 0.4485448512576866 1101 20-21 0.40930291551481446 1101 22-23 0.3485370014667595 1101 24-25 0.3608047620046122 1101 26-27 0.29510319636745663 1101 28-29 0.39650329417182917 1101 30-31 0.5475000394220686 1101 32-33 0.48625633780853406 1101 34-35 0.445918872884306 1101 36-37 0.309494355695044 1101 38-39 0.42422345466694367 1101 40-41 0.5253319596532506 1101 42-43 0.530565000524085 1101 44-45 0.46593774818391154 1101 46-47 0.44995855423556463 1101 48-49 0.2910812670673053 1101 50-51 0.3731372412520457 1101 52-53 0.3325822475376512 1101 54-55 0.35216768361789974 1101 56-57 0.41939572707645567 1101 58-59 0.4340588688770026 1101 60-61 0.5380447535983919 1101 62-63 0.40233988911339935 1101 64-65 0.504232434054579 1101 66-67 0.5436390098044157 1101 68-69 0.49618395003713545 1101 70-71 0.5134422766430049 1101 72-73 0.5483042346877518 1101 74-75 0.6705460753859533 1101 76-77 0.6315587496010302 1101 78-79 0.6601916463632307 1101 80-81 0.7229102056583763 1101 82-83 0.5885415587054936 1101 84-85 0.5151452865772939 1101 86-87 0.5685317254122886 1101 88-89 0.7805749650963882 1101 90-91 0.6706524375610492 1101 92-93 0.7200499189464615 1101 94-95 1.002946296950249 1101 96-97 1.1547039171650546 1101 98-99 1.2972547632635951 1101 100-101 1.6442657752285577 1102 1 0.1966993543229023 1102 2 0.23488928684327703 1102 3 0.1779173321606251 1102 4 0.09770529444534048 1102 5 0.05948583253329787 1102 6 0.1166303070265684 1102 7 0.1569455696824278 1102 8 0.15457284612283928 1102 9 0.10722985033655874 1102 10-11 0.13420991260785797 1102 12-13 0.14282532631423095 1102 14-15 0.21066997560049572 1102 16-17 0.30220439673544064 1102 18-19 0.2157144971497189 1102 20-21 0.3315570460331614 1102 22-23 0.34983542910075727 1102 24-25 0.2862397331441926 1102 26-27 0.27391760550452204 1102 28-29 0.2634502526299869 1102 30-31 0.1650668271214215 1102 32-33 0.16310605714612336 1102 34-35 0.16207943655988544 1102 36-37 0.1491727454838241 1102 38-39 0.22080859634295535 1102 40-41 0.3666508029492732 1102 42-43 0.329448068972674 1102 44-45 0.25466190696440094 1102 46-47 0.24472672316881017 1102 48-49 0.39763418630577974 1102 50-51 0.28075044069760224 1102 52-53 0.38954088570287126 1102 54-55 0.4757622802444459 1102 56-57 0.4356404735908086 1102 58-59 0.3816511867429284 1102 60-61 0.3438830187753936 1102 62-63 0.3137580469811141 1102 64-65 0.5719701817453782 1102 66-67 0.47836098936122085 1102 68-69 0.4622299932848719 1102 70-71 0.30263902411460464 1102 72-73 0.381441776739468 1102 74-75 0.5128665356278255 1102 76-77 0.3465709322873245 1102 78-79 0.26824376136440264 1102 80-81 0.37672940633438046 1102 82-83 0.4484126054211437 1102 84-85 0.37643473470848576 1102 86-87 0.33025130019894533 1102 88-89 0.5122028282394488 1102 90-91 0.47752048652051826 1102 92-93 0.7616945868901652 1102 94-95 0.847130745256159 1102 96-97 0.8283688779379652 1102 98-99 0.8006309716050026 1102 100-101 1.2857492780394217 1103 1 0.19997037262142925 1103 2 0.23563873455283613 1103 3 0.20267453277752168 1103 4 0.15456057350831998 1103 5 0.1405168441626543 1103 6 0.20541068300089194 1103 7 0.13851261565800144 1103 8 0.1304961090130874 1103 9 0.1678192380236041 1103 10-11 0.18435944785024105 1103 12-13 0.24067014312053914 1103 14-15 0.24637649054360367 1103 16-17 0.26144348639405735 1103 18-19 0.3355162978249737 1103 20-21 0.30100089997839063 1103 22-23 0.3375743728713374 1103 24-25 0.17915926546881877 1103 26-27 0.1528105236821773 1103 28-29 0.1684605689986185 1103 30-31 0.3100273331445109 1103 32-33 0.2611518868322591 1103 34-35 0.3374420725483773 1103 36-37 0.2881548637781819 1103 38-39 0.34384921574189775 1103 40-41 0.26578860462490894 1103 42-43 0.3285405411497351 1103 44-45 0.36844509649380797 1103 46-47 0.3092945819491888 1103 48-49 0.2781897071260886 1103 50-51 0.3430705815837669 1103 52-53 0.19425661243372616 1103 54-55 0.2996561362737893 1103 56-57 0.38978046358705853 1103 58-59 0.3706912434308549 1103 60-61 0.503305093720158 1103 62-63 0.35423207890975306 1103 64-65 0.473302347974375 1103 66-67 0.5020197572325102 1103 68-69 0.5310170384268034 1103 70-71 0.5295739163658695 1103 72-73 0.5419563447024451 1103 74-75 0.44105835756105094 1103 76-77 0.38665690202597247 1103 78-79 0.46415920882386885 1103 80-81 0.5430036341698177 1103 82-83 0.5438045232236526 1103 84-85 0.6402313667788455 1103 86-87 0.6824767180639775 1103 88-89 0.8453707870573375 1103 90-91 0.8927624522576671 1103 92-93 0.9929887180226729 1103 94-95 0.9592479975591068 1103 96-97 1.020249791613928 1103 98-99 1.359292344619881 1103 100-101 1.6515993884975053 1104 1 0.12891470727228693 1104 2 0.13745691637101487 1104 3 0.11161722052455758 1104 4 0.11213139037261755 1104 5 0.14506559251574913 1104 6 0.06191430618930127 1104 7 0.11454193054601092 1104 8 0.05864992982995432 1104 9 0.06534888229237623 1104 10-11 0.13805674692797254 1104 12-13 0.15674557131553257 1104 14-15 0.04402635879129946 1104 16-17 0.12322131906862666 1104 18-19 0.08246046778544525 1104 20-21 0.040056400637148215 1104 22-23 0.053608628470811936 1104 24-25 -0.06262515482104192 1104 26-27 0.03586553026978834 1104 28-29 0.2363862936363006 1104 30-31 0.14646590363199863 1104 32-33 0.19996776298508934 1104 34-35 0.13141440456418962 1104 36-37 0.1286621101549983 1104 38-39 0.18061057937826064 1104 40-41 0.32649265600830546 1104 42-43 0.3728331959586981 1104 44-45 0.30136341006429035 1104 46-47 0.32891414716657863 1104 48-49 0.2653043316320165 1104 50-51 0.40941692545728614 1104 52-53 0.5208961051873544 1104 54-55 0.5169115710563972 1104 56-57 0.4512396203722986 1104 58-59 0.38919503130964017 1104 60-61 0.30484247843688905 1104 62-63 0.29197499722332054 1104 64-65 0.4333863400692408 1104 66-67 0.46540167422855205 1104 68-69 0.36828811879570367 1104 70-71 0.5713492786847141 1104 72-73 0.4058998559013993 1104 74-75 0.5717813483383871 1104 76-77 0.372472119417278 1104 78-79 0.39583905072109715 1104 80-81 0.41298963548734235 1104 82-83 0.455057817031296 1104 84-85 0.382299054525884 1104 86-87 0.4948606113446097 1104 88-89 0.5160328423933045 1104 90-91 0.6389638685949528 1104 92-93 0.795968790486441 1104 94-95 0.6829494138963916 1104 96-97 0.9064396796244694 1104 98-99 0.8380901048306839 1104 100-101 1.2597194477860434 1105 1 0.1226387664038171 1105 2 0.15559163139692345 1105 3 0.08616487663056205 1105 4 0.06516113874619123 1105 5 0.10434397558046271 1105 6 0.12849593971309758 1105 7 0.16579711871970204 1105 8 0.11501094650719779 1105 9 0.20465390594635835 1105 10-11 0.17974922646540392 1105 12-13 0.1787601101483247 1105 14-15 0.16622811560247897 1105 16-17 0.28331583684153827 1105 18-19 0.33964017912501987 1105 20-21 0.3334278900867247 1105 22-23 0.31483061403949364 1105 24-25 0.24546991078205593 1105 26-27 0.14312611482677795 1105 28-29 0.20876556193314855 1105 30-31 0.22188908538191043 1105 32-33 0.06095052084073416 1105 34-35 0.14190509657098715 1105 36-37 0.09646386048096645 1105 38-39 0.2322253344418499 1105 40-41 0.2408733903055449 1105 42-43 0.35041642433390763 1105 44-45 0.4063902213171673 1105 46-47 0.46731742697980394 1105 48-49 0.4872817937958942 1105 50-51 0.5512700634490528 1105 52-53 0.4850926934511577 1105 54-55 0.4894877424914057 1105 56-57 0.5045037325460768 1105 58-59 0.43466674978978403 1105 60-61 0.4021110287178047 1105 62-63 0.3202412640251495 1105 64-65 0.46855434985850053 1105 66-67 0.398549432220733 1105 68-69 0.47222758953373045 1105 70-71 0.41797005390708364 1105 72-73 0.4623684973166782 1105 74-75 0.558462973670327 1105 76-77 0.6062836095153763 1105 78-79 0.6024081490028586 1105 80-81 0.7869956266333169 1105 82-83 0.5847760258143282 1105 84-85 0.7318540704999954 1105 86-87 0.8137908961139075 1105 88-89 0.8210078572410389 1105 90-91 0.9567238276698227 1105 92-93 1.1355664382299295 1105 94-95 1.312208430908143 1105 96-97 1.2406949164372918 1105 98-99 1.4083808985529949 1105 100-101 1.570426519915312 1106 1 -0.4520042920581524 1106 2 -0.29547900017146134 1106 3 -0.32432497045149233 1106 4 -0.2034349555527868 1106 5 -0.11613740322929544 1106 6 -0.2188267735316458 1106 7 -0.2158465497667379 1106 8 -0.182531710162273 1106 9 -0.4525483725079411 1106 10-11 -0.5436149684438476 1106 12-13 -0.5725365136107072 1106 14-15 -0.5632633504896774 1106 16-17 -0.41144026919461396 1106 18-19 -0.5510187245297189 1106 20-21 -0.5017226469664706 1106 22-23 -0.642224434878294 1106 24-25 -0.543094832891839 1106 26-27 -0.5274477972318863 1106 28-29 -0.5687016267291511 1106 30-31 -0.6070827016294587 1106 32-33 -0.8127937167445012 1106 34-35 -1.0656254883060612 1106 36-37 -0.8873344556455649 1106 38-39 -0.8920188915606317 1106 40-41 -0.9643380719771812 1106 42-43 -1.18029328895458 1106 44-45 -1.1217275314247246 1106 46-47 -1.1554557906290555 1106 48-49 -1.2856565422529442 1106 50-51 -1.344841738336747 1106 52-53 -1.3930285341137179 1106 54-55 -1.4015920008350093 1106 56-57 -1.4637801424735457 1106 58-59 -1.531732998993391 1106 60-61 -1.3056293325093478 1106 62-63 -1.597599764110619 1106 64-65 -2.115361338066357 1106 66-67 -1.8925120361107588 1106 68-69 -1.8080838060838786 1106 70-71 -1.8459774994314486 1106 72-73 -1.7846283547511028 1106 74-75 -1.8941378669794382 1106 76-77 -1.6507040020972248 1106 78-79 -1.7196075571969978 1106 80-81 -1.7802228934009712 1106 82-83 -1.7545275681822083 1106 84-85 -1.731867490647879 1106 86-87 -1.612267444876906 1106 88-89 -1.9635698488095308 1106 90-91 -2.1114650688551 1106 92-93 -2.182884356989746 1106 94-95 -2.029135012873084 1106 96-97 -2.2355102679985883 1106 98-99 -2.2660603627820066 1106 100-101 -1.5874617143340863 1107 1 0.1243894073923073 1107 2 0.14439803652420125 1107 3 0.05481996991577631 1107 4 0.16382838324764748 1107 5 0.09756306027301775 1107 6 0.09364907457580784 1107 7 0.09440821598873583 1107 8 0.09499086509472932 1107 9 0.21223370340504033 1107 10-11 0.18224936908266898 1107 12-13 0.23450124978314335 1107 14-15 0.1787358186257677 1107 16-17 0.1824041461268564 1107 18-19 0.24650524425431541 1107 20-21 0.26057279170562 1107 22-23 0.2294841600683455 1107 24-25 0.24279377959040715 1107 26-27 0.399628052730435 1107 28-29 0.3522339055232706 1107 30-31 0.2595213276612469 1107 32-33 0.3379825800739127 1107 34-35 0.3747750722437573 1107 36-37 0.2667187699533926 1107 38-39 0.3314527885692584 1107 40-41 0.13885379458095315 1107 42-43 0.22097722246746088 1107 44-45 0.33992764577794077 1107 46-47 0.2067323512167789 1107 48-49 0.07615230340964985 1107 50-51 0.10320146195279989 1107 52-53 0.07704024096413065 1107 54-55 0.13093306303299812 1107 56-57 0.26732942133108395 1107 58-59 0.23752008715774053 1107 60-61 0.1822965641932015 1107 62-63 0.2781468489171459 1107 64-65 0.17183840263297867 1107 66-67 0.3013048653747248 1107 68-69 0.1834766754867303 1107 70-71 0.1474748258919547 1107 72-73 0.14962225705720655 1107 74-75 0.22722181114911066 1107 76-77 0.12313488192065947 1107 78-79 0.27423286302988004 1107 80-81 0.3127122811953633 1107 82-83 0.2954352512797058 1107 84-85 0.3986453534623422 1107 86-87 0.5228283434610788 1107 88-89 0.6065769970751766 1107 90-91 0.6402784451207175 1107 92-93 0.6300291679987424 1107 94-95 0.8419650435576287 1107 96-97 0.8350530902779276 1107 98-99 0.9195732532756118 1107 100-101 1.3294099981846124 1108 1 0.06751668886442985 1108 2 0.049305257603244 1108 3 0.04099853105412876 1108 4 0.08154678634761581 1108 5 -0.05106652507473797 1108 6 0.034652389162033614 1108 7 0.03634760057829567 1108 8 0.11919709996526251 1108 9 0.10685413677414601 1108 10-11 -0.004249987737864558 1108 12-13 0.1229859552704724 1108 14-15 0.0496358615043917 1108 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52-53 -0.271284756309889 2316 54-55 -0.10850269927269096 2316 56-57 -0.014256819354720562 2316 58-59 -0.08246503848947384 2316 60-61 0.13997005796939277 2316 62-63 0.10922441803129601 2316 64-65 0.12004392100808303 2316 66-67 0.21297225978613454 2316 68-69 0.2589230649845149 2316 70-71 0.1360550195323711 2316 72-73 0.19727299434494583 2316 74-75 0.47221259658045867 2316 76-77 0.42320184584619724 2316 78-79 0.31419240596380504 2316 80-81 0.302845309348573 2316 82-83 0.17758644355052056 2316 84-85 0.20876558536924605 2316 86-87 0.40049510417225065 2316 88-89 0.6227022477314925 2316 90-91 0.3099432489890255 2316 92-93 0.4373732941207322 2316 94-95 0.7086913070585972 2316 96-97 0.6751170030541722 2316 98-99 0.5696549595330573 2316 100-101 0.8463491331348933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 11.0 5 36.0 6 63.0 7 215.0 8 529.0 9 1106.0 10 1805.0 11 2983.0 12 4656.0 13 6789.0 14 8793.0 15 12331.0 16 16887.0 17 23949.0 18 21549.0 19 13125.0 20 11244.0 21 11345.0 22 11918.0 23 12868.0 24 14679.0 25 17459.0 26 20978.0 27 26235.0 28 32655.0 29 40620.0 30 51109.0 31 63996.0 32 81114.0 33 105780.0 34 143850.0 35 204149.0 36 309600.0 37 452646.0 38 333933.0 39 26157.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62818930524395 20.218923910089547 15.568779905166203 21.584106879500304 2 15.385179268972212 21.420381942830748 39.85916822339904 23.335270564797998 3 19.74097953641556 24.974331283828686 30.559307311188206 24.72538186856755 4 12.901099143290129 17.37610382666298 37.51000756526418 32.212789464782716 5 13.016488631991233 38.46512115989196 34.280216181198135 14.23817402691867 6 31.12385137585756 37.313617331785466 17.032001690326286 14.530529602030692 7 27.735245459044805 33.83081692875189 20.65321911812688 17.78071849407643 8 26.09800538623812 35.15811593779331 19.500175117419705 19.24370355854887 9 25.94349001591149 17.379313913426355 21.032871767574388 35.644324303087764 10-11 24.755159016678558 27.43079028982292 26.877265925983064 20.936784767515455 12-13 25.95825514228196 24.802093687433683 27.788746286299393 21.450904883984965 14-15 22.61786408492644 25.998732255287184 27.095623693506667 24.287779966279714 16-17 21.208779731033058 29.497271665809844 27.541274768574304 21.752673834582794 18-19 21.151549154333786 27.951902359701243 30.264841740326982 20.631706745637995 20-21 22.167711902512902 26.386585462553697 30.57309460160979 20.872608033323615 22-23 21.798402264532616 26.47118079919354 30.529434635840452 21.20098230043339 24-25 21.94707573073792 26.561958747031266 30.60077492452703 20.890190597703782 26-27 21.529399138443612 26.628737806955215 30.883840331782096 20.958022722819074 28-29 21.229500361858726 27.466143271140588 30.515530698580186 20.7888256684205 30-31 21.516948060317052 26.69105693783595 30.42699903362015 21.36499596822685 32-33 21.40852378650336 26.84147585415857 30.430927796524575 21.319072562813492 34-35 22.06055469341036 26.76491288926494 30.327414476341417 20.847117940983274 36-37 21.876617774976527 26.440393174567696 30.552838552016382 21.13015049843939 38-39 21.502814096209654 27.059498239483016 30.496854354530605 20.94083330977673 40-41 21.80090438335582 27.036228037225563 30.083299475653135 21.079568103765485 42-43 21.50400282933218 27.114097947895527 29.751261734072965 21.630637488699325 44-45 21.81857643671745 26.918637715836415 29.704226438562053 21.558559408884083 46-47 22.194132632160947 27.50078934596159 29.176981665613404 21.128096356264052 48-49 22.216219945562678 27.502825595027915 28.908388435830123 21.372566023579285 50-51 21.82612253619852 27.819258699934025 29.08084675381174 21.273772010055715 52-53 22.285500325560292 28.112310874384516 28.213284872202614 21.38890392785258 54-55 21.961089915488476 27.760063741782538 28.49687447746256 21.78197186526643 56-57 22.229251939587126 27.864798811405482 28.423401819975457 21.48254742903193 58-59 22.681155497081935 27.548006368428844 28.31150394481334 21.459334189675882 60-61 22.154725223759815 27.727459800850053 29.001456996096632 21.116357979293504 62-63 21.853647832400892 27.81497059895907 29.056723191343693 21.274658377296348 64-65 21.817594245991344 28.141968243138678 28.6524439061294 21.38799360474058 66-67 21.99091497534222 27.743174852467767 28.355966042073216 21.909944130116795 68-69 21.9056081173991 27.80059707613799 28.59123665478296 21.702558151679956 70-71 22.28540450207482 27.763657122487807 28.372615372674304 21.57832300276307 72-73 22.24671576981474 27.52946452398965 28.560740830530897 21.663078875664716 74-75 21.88917438964033 28.511343823769113 28.037760202685835 21.56172158390472 76-77 22.038922541564634 27.876824658832454 28.1772073299217 21.90704546968121 78-79 22.124972031520176 27.954777064265457 28.14697502025469 21.773275883959677 80-81 22.17051214299164 27.956501887003988 28.121438061375905 21.751547908628474 82-83 22.176921593499323 27.879794027072574 28.389647692386934 21.55363668704117 84-85 21.96728867407352 28.02566828624638 28.262137145709946 21.744905893970152 86-87 21.93068991472189 28.189664383033303 28.017014418080745 21.862631284164067 88-89 22.045917656004228 28.11130472778704 27.83583616292101 22.00694145328773 90-91 22.15549265134844 27.674845677718295 28.08487036688752 22.08479130404574 92-93 22.310829318823362 27.87803172463061 28.08063181906712 21.73050713747891 94-95 22.043354377767802 27.833416619912793 28.14522624164479 21.978002760674617 96-97 21.726154684977896 27.80931701331611 28.41017817898007 22.05435012272593 98-99 21.794770749871777 28.034147779989443 27.792671728879885 22.37840974125889 100-101 21.6847488708755 27.786537429000703 28.218732577365184 22.30998112275861 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 514.0 1 362.0 2 283.5 3 390.0 4 362.0 5 369.5 6 697.5 7 1597.5 8 2645.5 9 3235.0 10 3157.0 11 2939.5 12 3093.0 13 3361.5 14 3662.5 15 3893.5 16 4104.5 17 4389.5 18 4557.0 19 4751.5 20 5114.0 21 5765.0 22 6728.5 23 8064.5 24 9691.0 25 11811.0 26 14803.5 27 18179.0 28 20632.5 29 23323.0 30 27145.0 31 30926.0 32 34938.5 33 38793.5 34 43250.0 35 47250.5 36 51743.0 37 56407.5 38 59234.5 39 61362.0 40 63120.5 41 65834.5 42 68447.0 43 71466.0 44 75443.0 45 77662.0 46 78464.5 47 85594.5 48 97464.0 49 93723.0 50 80119.0 51 71887.5 52 66343.0 53 63218.0 54 61561.5 55 57299.5 56 50380.5 57 44529.0 58 40047.5 59 35371.5 60 28965.0 61 22668.0 62 17719.5 63 13126.5 64 9477.5 65 7056.0 66 5089.0 67 3649.5 68 2566.5 69 1773.5 70 1162.0 71 769.0 72 575.5 73 440.5 74 312.0 75 193.0 76 133.0 77 92.5 78 53.5 79 29.5 80 20.5 81 18.0 82 12.5 83 8.0 84 4.5 85 2.5 86 3.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03756280630576028 2 0.0 3 0.0 4 0.0 5 0.010444759916652733 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006492041140855254 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.1977935684234786E-4 22-23 0.0014133964107397045 24-25 0.0 26-27 8.624113692649045E-4 28-29 0.0014613081534766436 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.114263277901044E-4 38-39 0.0 40-41 1.4373522821081744E-4 42-43 0.022015445787623534 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.6769109957928699E-4 84-85 1.9164697094775656E-4 86-87 0.0 88-89 0.0 90-91 0.010708274501705898 92-93 0.002611189979163183 94-95 0.0 96-97 0.0 98-99 3.593380705270436E-4 100-101 0.01276847943939428 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2087171.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.75368332213684 #Duplication Level Percentage of deduplicated Percentage of total 1 71.52601619304411 35.58682758962748 2 15.018390935329261 14.94440533208845 3 5.505383428309509 8.21739310977154 4 2.722138249892682 5.417456177769451 5 1.4387313540073279 3.5791092086454865 6 0.8847678815919406 2.641227659659396 7 0.6011337272330951 2.0936031969307844 8 0.39759016241682843 1.5825260026307069 9 0.2810395824028852 1.2584478945472857 >10 1.4795775455204814 13.25294231120263 >50 0.09553077397231104 3.257477800873527 >100 0.04536764731852131 3.9895696696469773 >500 0.0025032331774924436 0.8614686742949785 >1k 0.0014441729870148664 1.534155762435788 >5k 2.888345974029733E-4 1.2293077506912802 >10k+ 9.627819913432443E-5 0.5540818591842745 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11567 0.5541951282381751 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9617 0.4607672299011437 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9448 0.45267014537860095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6598 0.31612167857832446 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3466 0.16606210032623106 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3257 0.15604854609421076 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2592 0.12418723717414624 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2509 0.12021056252698029 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2508 0.12016265078424336 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2439 0.11685674053539456 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2218 0.10626824539053102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.582348547387828E-5 0.0 0.0 0.0 0.0 3 9.582348547387828E-5 0.0 0.0 0.0 0.0 4 9.582348547387828E-5 0.0 0.0 0.0 0.0 5 1.9164697094775656E-4 0.0 0.0 0.0 0.0 6 1.9164697094775656E-4 0.0 0.0 0.0 0.0 7 2.874704564216348E-4 0.0 0.0 0.0 0.0 8 3.3538219915857397E-4 0.0 0.0 0.0 0.0 9 3.3538219915857397E-4 0.0 0.0 0.0 0.0 10-11 3.8329394189551313E-4 0.0 0.0 2.395587136846957E-5 0.0 12-13 4.3120568463245223E-4 0.0 0.0 4.791174273693914E-5 0.0 14-15 5.270291701063305E-4 0.0 0.0 9.582348547387828E-5 0.0 16-17 5.270291701063305E-4 0.0 0.0 9.582348547387828E-5 0.0 18-19 5.509850414748001E-4 0.0 0.0 1.1977935684234785E-4 0.0 20-21 5.749409128432696E-4 0.0 0.0 1.6769109957928699E-4 0.0 22-23 6.707643983171479E-4 0.0 0.0 2.395587136846957E-4 0.0 24-25 7.665878837910263E-4 0.0 0.0 2.395587136846957E-4 0.0 26-27 7.665878837910263E-4 0.0 0.0 5.749409128432696E-4 0.0 28-29 0.0010301024688441915 0.0 0.0 0.001389440539371235 0.0 30-31 0.0011977935684234784 0.0 0.0 0.0030903074065325746 0.0 32-33 0.0012457053111604175 0.0 0.0 0.005677541514327289 0.0 34-35 0.0013415287966342959 0.0 0.0 0.008264775622122002 0.0 36-37 0.001389440539371235 0.0 0.0 0.01123530367181223 0.0 38-39 0.001437352282108174 0.0 0.0 0.014565169792029497 0.0 40-41 0.0014613081534766439 0.0 0.0 0.01938029993709188 0.0 42-43 0.001557131638950522 0.0 0.0 0.02551300300742009 0.0 44-45 0.0016769109957928698 0.0 0.0 0.03140614736406361 0.0 46-47 0.0018925138381090962 0.0 0.0 0.03885642335965764 0.0 48-49 0.002012293194951444 0.0 0.0 0.04544428798598678 0.0 50-51 0.002012293194951444 0.0 0.0 0.055146415890216946 0.0 52-53 0.002012293194951444 0.0 0.0 0.08030008082710999 0.0 54-55 0.002108116680425322 0.0 0.0 0.11769519603329098 0.0 56-57 0.0021560284231622612 0.0 0.0 0.14716091781650859 2.395587136846957E-5 58-59 0.0021560284231622612 0.0 0.0 0.1888680898690141 4.791174273693914E-5 60-61 0.0021560284231622612 0.0 0.0 0.2412116688091201 4.791174273693914E-5 62-63 0.002179984294530731 0.0 0.0 0.27534878550918923 4.791174273693914E-5 64-65 0.0022997636513730785 0.0 0.0 0.30222727318461207 7.18676141054087E-5 66-67 0.0024434988795838963 0.0 0.0 0.3817847219992995 9.582348547387828E-5 68-69 0.0025393223650577745 0.0 0.0 0.4639294049217817 9.582348547387828E-5 70-71 0.002563278236426244 0.0 0.0 0.5040554894639682 9.582348547387828E-5 72-73 0.002611189979163183 0.0 0.0 0.5867751132992937 9.582348547387828E-5 74-75 0.0026591017219001222 0.0 0.0 0.7079918224237496 9.582348547387828E-5 76-77 0.0026830575932685918 0.0 0.0 0.8202490356563981 9.582348547387828E-5 78-79 0.0026830575932685918 0.0 0.0 0.9420406856936974 9.582348547387828E-5 80-81 0.0029226163069532873 0.0 0.0 1.1019700829496002 9.582348547387828E-5 82-83 0.003018439792427166 0.0 0.0 1.2717692992093126 9.582348547387828E-5 84-85 0.0031621750206379832 0.0 0.0 1.4278418011748917 9.582348547387828E-5 86-87 0.0033538219915857396 0.0 0.0 1.658848268781044 9.582348547387828E-5 88-89 0.0034017337343226787 0.0 0.0 1.9400901986468764 9.582348547387828E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGTG 25 0.005020655 57.003426 9 TTTACGC 95 0.0012266322 25.000902 3 GTACATG 9135 0.0 23.0945 1 GAGTACT 10155 0.0 22.663841 12-13 TACATGG 9150 0.0 22.634697 2 ACATGGG 9160 0.0 21.624687 3 GTACTTT 10745 0.0 21.39472 14-15 AGTACTT 10730 0.0 21.294382 12-13 AGAGTAC 15770 0.0 21.070543 10-11 GTATCAA 21930 0.0 20.648495 1 ACGACGG 120 0.0047637834 19.792381 5 GTATAGG 530 0.0 19.723362 1 TACTTTT 12205 0.0 18.67975 14-15 CATGGGA 5320 0.0 18.482811 4 CATGGGG 3975 0.0 18.40318 4 ACTTTTT 12650 0.0 18.28547 16-17 AGGACCG 780 0.0 18.26989 5 CAACGCA 25050 0.0 17.995659 5 TCAACGC 25060 0.0 17.98848 4 ATCAACG 25100 0.0 17.978737 3 >>END_MODULE