FastQCFastQC Report
Thu 26 May 2016
SRR937716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912739
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94770.49546749451963906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72220.37757373065535865No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65610.34301595774436555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49080.25659538494274436No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46640.24383880916319478No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36800.19239425765878146No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC29370.15354943878908728No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28920.15119679161662936No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22030.11517514935388466No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20000.10456209655368556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86200.026.4836441
TACATGG85600.025.9930022
ACATGGG85900.024.7392963
GAGTACT80950.022.76614812-13
GTATCAA164450.022.4714411
TATACCG1954.185913E-721.9439625
AGTACTT84300.021.52338212-13
GTACTTT85750.021.2685414-15
AGAGTAC128900.021.1148410-11
CATGGGA47900.021.0424394
GTAAGCG2750.020.72202794-95
GTATAGG4950.020.1770611
CATGGGG37400.019.8311884
ACTACGC1707.944028E-519.5630958
GGTATCA128550.019.5346491
ATCAACG189450.019.3729463
TCAACGC189700.019.3233664
TATCAAC189850.019.307592
CAACGCA190350.019.2578875
AACGCAG194000.018.9786646
Produced by FastQC (version 0.11.5)