##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937716_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1912739 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.63798144963845 33.0 31.0 34.0 30.0 34.0 2 31.739813429851118 33.0 31.0 34.0 30.0 34.0 3 31.780985278179617 33.0 31.0 34.0 30.0 34.0 4 35.40639993224376 37.0 35.0 37.0 33.0 37.0 5 35.41279076758512 37.0 35.0 37.0 33.0 37.0 6 35.473172764292464 37.0 35.0 37.0 33.0 37.0 7 35.39448455853098 37.0 35.0 37.0 33.0 37.0 8 35.39917939666625 37.0 35.0 37.0 33.0 37.0 9 36.94961727658609 39.0 37.0 39.0 33.0 39.0 10-11 36.86222872017562 39.0 37.0 39.0 33.0 39.0 12-13 36.75413216335318 39.0 37.0 39.0 33.0 39.0 14-15 37.83226697421865 40.0 38.0 41.0 33.0 41.0 16-17 37.74319235400125 40.0 37.0 41.0 33.0 41.0 18-19 37.68246085848618 40.0 37.0 41.0 32.0 41.0 20-21 37.698544861583315 40.0 37.5 41.0 32.5 41.0 22-23 37.70372460644134 40.0 37.5 41.0 32.0 41.0 24-25 37.618318547381534 40.0 37.0 41.0 32.0 41.0 26-27 37.51187407168464 40.0 37.0 41.0 32.0 41.0 28-29 37.36334622758254 40.0 37.0 41.0 32.0 41.0 30-31 37.117469241752275 39.0 36.5 41.0 31.0 41.0 32-33 36.96438562710333 39.0 36.0 41.0 31.0 41.0 34-35 36.913810770837 39.0 36.0 41.0 31.0 41.0 36-37 36.73128142417758 39.0 36.0 41.0 30.0 41.0 38-39 36.550206274875976 39.0 35.5 41.0 30.0 41.0 40-41 36.32413256591725 39.0 35.0 41.0 30.0 41.0 42-43 36.033729902511524 39.0 35.0 40.0 29.5 41.0 44-45 35.74697384222311 39.0 35.0 40.0 28.5 41.0 46-47 35.33065331966358 38.5 35.0 40.0 26.5 41.0 48-49 34.923778152690986 38.0 34.5 40.0 24.5 41.0 50-51 34.03020511423671 37.5 33.5 39.5 23.5 40.5 52-53 34.15140591580974 38.0 33.5 39.5 24.0 40.5 54-55 34.41621935873112 38.0 34.0 40.0 23.5 41.0 56-57 34.14427059834091 38.0 34.0 40.0 22.5 41.0 58-59 33.84064788766267 38.0 33.0 40.0 22.0 41.0 60-61 33.46495915020293 37.5 33.0 40.0 20.0 41.0 62-63 33.075634992542106 37.0 32.5 40.0 18.5 41.0 64-65 32.62130954615345 36.0 32.0 39.0 15.5 41.0 66-67 32.09128558574902 36.0 31.0 39.0 11.0 40.0 68-69 31.590404388680316 35.0 31.0 38.5 8.0 40.0 70-71 31.078074164849465 35.0 30.0 38.0 7.0 40.0 72-73 30.578640891412785 35.0 29.5 37.0 2.0 39.5 74-75 30.01764197833578 34.0 29.0 37.0 2.0 39.0 76-77 29.45433485697735 34.0 29.0 36.0 2.0 39.0 78-79 28.896170360932672 34.0 28.0 36.0 2.0 38.0 80-81 28.30126248275379 34.0 26.5 35.0 2.0 37.0 82-83 27.720991991066214 33.0 26.0 35.0 2.0 37.0 84-85 27.13323563748112 33.0 25.5 35.0 2.0 36.0 86-87 26.52871066047171 32.0 24.5 35.0 2.0 36.0 88-89 25.919829626519874 32.0 23.5 34.5 2.0 35.0 90-91 25.282998621348757 31.5 20.0 34.0 2.0 35.0 92-93 24.551010880208956 31.0 17.5 34.0 2.0 35.0 94-95 23.705882768114208 31.0 4.5 34.0 2.0 35.0 96-97 22.739043330010002 30.5 2.0 34.0 2.0 35.0 98-99 21.500117370953383 29.5 2.0 34.0 2.0 35.0 100-101 18.34226677032256 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15274584625828425 1101 2 -0.0034891663803371387 1101 3 0.07130327990892482 1101 4 0.13037708728570863 1101 5 0.06312120480117045 1101 6 0.07041068538051576 1101 7 0.020264352499879124 1101 8 0.13426368986631587 1101 9 0.24537713103616454 1101 10-11 0.29960469508137066 1101 12-13 0.2791451549358115 1101 14-15 0.3663300605184574 1101 16-17 0.38536368431689283 1101 18-19 0.587801580326051 1101 20-21 0.5285829666750743 1101 22-23 0.3861360809995418 1101 24-25 0.3408330154757593 1101 26-27 0.4381082269037293 1101 28-29 0.37305862991009064 1101 30-31 0.32998168655287685 1101 32-33 0.36141676476950835 1101 34-35 0.5209248455322069 1101 36-37 0.49667762822472383 1101 38-39 0.47954305207834125 1101 40-41 0.5307591685599391 1101 42-43 0.5232125387486946 1101 44-45 0.7522872462572394 1101 46-47 0.8563060547450903 1101 48-49 0.7978374124758787 1101 50-51 0.6136751060991799 1101 52-53 0.8460428193278418 1101 54-55 0.9097921699335387 1101 56-57 0.9830552198498665 1101 58-59 0.9557950014282781 1101 60-61 0.8939489624896595 1101 62-63 0.8194035910816098 1101 64-65 0.7831640833344622 1101 66-67 0.9127301384271327 1101 68-69 1.0324907556188556 1101 70-71 1.0441204968489792 1101 72-73 1.1266567686070807 1101 74-75 1.1214051720227367 1101 76-77 0.9347595490692555 1101 78-79 1.3218723769272245 1101 80-81 1.4649195583607835 1101 82-83 1.4373947098313984 1101 84-85 1.5561640078083663 1101 86-87 1.913230262925964 1101 88-89 1.9162700739760048 1101 90-91 2.025307031185573 1101 92-93 2.1681956914587666 1101 94-95 2.3889088016904836 1101 96-97 2.4763981038734606 1101 98-99 2.7474624887848584 1101 100-101 3.1761691721888674 1102 1 0.2119572913760166 1102 2 0.2408072971227675 1102 3 0.11786802403946339 1102 4 0.1673161627329307 1102 5 0.14213627077967317 1102 6 0.1687274356157218 1102 7 0.2371644463202145 1102 8 0.09308803306924318 1102 9 0.3098207125300547 1102 10-11 0.4182486738341069 1102 12-13 0.3198756795547837 1102 14-15 0.4405284614136704 1102 16-17 0.5124754627310395 1102 18-19 0.3971981049896769 1102 20-21 0.5914405116819097 1102 22-23 0.5149445496091332 1102 24-25 0.6066243175470234 1102 26-27 0.5939235334965929 1102 28-29 0.5428112690721747 1102 30-31 0.7259114907297359 1102 32-33 0.6000708741921272 1102 34-35 0.6370567333698389 1102 36-37 0.6858219193470703 1102 38-39 0.6517554651697566 1102 40-41 0.7567592438613815 1102 42-43 0.7995549072327606 1102 44-45 0.8345595371239227 1102 46-47 0.9390527008760685 1102 48-49 1.0415300416473983 1102 50-51 0.9697745004917664 1102 52-53 0.9872165935890251 1102 54-55 1.0741579107573784 1102 56-57 1.2467710261027776 1102 58-59 1.1571448015511194 1102 60-61 1.118552787189671 1102 62-63 1.2133286039857367 1102 64-65 1.326843075065753 1102 66-67 1.4739075201524408 1102 68-69 1.733671893659956 1102 70-71 1.7367561258983493 1102 72-73 1.6547914863777358 1102 74-75 1.6467868999542752 1102 76-77 1.5782421660393098 1102 78-79 1.7226471936754884 1102 80-81 1.8536670503195083 1102 82-83 2.173992586593595 1102 84-85 2.2893686136294313 1102 86-87 2.16444723481883 1102 88-89 2.1976089335007387 1102 90-91 2.378076744948178 1102 92-93 2.486788951192242 1102 94-95 2.4567480620202424 1102 96-97 2.803920275111551 1102 98-99 3.1366137409120114 1102 100-101 3.6067135577882574 1103 1 0.1070605391102859 1103 2 0.06546832034941374 1103 3 0.26226928709508357 1103 4 0.07453187616469847 1103 5 0.15258335319353478 1103 6 0.16297546790529793 1103 7 0.07072258701689549 1103 8 0.1445650432863559 1103 9 0.1810405465688163 1103 10-11 0.15156275642195993 1103 12-13 0.20963178509882852 1103 14-15 0.3060803075041747 1103 16-17 0.2874372627058932 1103 18-19 0.36484177928945627 1103 20-21 0.404746179066926 1103 22-23 0.2886979023396421 1103 24-25 0.2016805796810246 1103 26-27 0.19149100585217838 1103 28-29 0.15348733630422373 1103 30-31 0.2810325766268207 1103 32-33 0.23012193388752422 1103 34-35 0.26835672286160417 1103 36-37 0.48584694100954806 1103 38-39 0.6781408297797356 1103 40-41 0.6135644900555874 1103 42-43 0.44957333971390057 1103 44-45 0.44282086319451963 1103 46-47 0.4239763955721685 1103 48-49 0.3668928342199749 1103 50-51 0.4035749766354826 1103 52-53 0.36523598903809784 1103 54-55 0.452708747368078 1103 56-57 0.5046545724432363 1103 58-59 0.5910764153234638 1103 60-61 0.748994675778242 1103 62-63 0.9336846092708626 1103 64-65 0.7751612834285808 1103 66-67 0.913849285570695 1103 68-69 0.9312183495417266 1103 70-71 1.057447921032523 1103 72-73 0.9442369960013863 1103 74-75 0.9981261402948753 1103 76-77 0.9718736146083806 1103 78-79 1.252497261806763 1103 80-81 1.3093502838245925 1103 82-83 1.4900764767773502 1103 84-85 1.6431846109558599 1103 86-87 1.6033842577491768 1103 88-89 1.7854356147755759 1103 90-91 1.76183540347699 1103 92-93 1.9260763204637463 1103 94-95 2.1502710259632067 1103 96-97 2.3311144294310395 1103 98-99 2.597304536902346 1103 100-101 3.1413982762278003 1104 1 0.21198179652516203 1104 2 0.2695899075687862 1104 3 0.24168029009186753 1104 4 0.21338890626816465 1104 5 0.25969053436931233 1104 6 0.2835047406969551 1104 7 0.30579461723488066 1104 8 0.24802402649486055 1104 9 0.5224930252997737 1104 10-11 0.35653814440911447 1104 12-13 0.3564824340490702 1104 14-15 0.5292180660668464 1104 16-17 0.5908529943098628 1104 18-19 0.5221789603419111 1104 20-21 0.5405575386471924 1104 22-23 0.5093379027624678 1104 24-25 0.5097423352204871 1104 26-27 0.4045599934827493 1104 28-29 0.589849559242893 1104 30-31 0.5258575794016238 1104 32-33 0.533466754001374 1104 34-35 0.6539020542428915 1104 36-37 0.6571957480228221 1104 38-39 0.6666370921716336 1104 40-41 0.7501045642968336 1104 42-43 0.7751090517973935 1104 44-45 0.9032234026186572 1104 46-47 0.8555472480427966 1104 48-49 0.8259335206784257 1104 50-51 0.6964458173022052 1104 52-53 0.903567173004518 1104 54-55 1.0780809225802415 1104 56-57 0.9970016370599026 1104 58-59 1.1645473257565229 1104 60-61 1.3214545469219345 1104 62-63 1.380769662538171 1104 64-65 1.3837899785338479 1104 66-67 1.4719057557817017 1104 68-69 1.3226984441138718 1104 70-71 1.5292147975267234 1104 72-73 1.5835653012255797 1104 74-75 1.5490196691148945 1104 76-77 1.5311404250359821 1104 78-79 1.635698329795467 1104 80-81 1.785339036645638 1104 82-83 1.781524462979121 1104 84-85 2.036761659593214 1104 86-87 2.0805562634784778 1104 88-89 2.1451451595502355 1104 90-91 2.267455469402652 1104 92-93 2.5054835356417975 1104 94-95 2.4847583226762637 1104 96-97 2.5050226567494924 1104 98-99 2.72169496235367 1104 100-101 3.097291109146237 1105 1 -0.009599548645081057 1105 2 0.05554558600768189 1105 3 -0.07701613348923075 1105 4 -0.0857779174585076 1105 5 -0.12778517548778012 1105 6 -0.1263814983394056 1105 7 -0.08514461400356765 1105 8 -0.10160548209454134 1105 9 -0.011602768617684944 1105 10-11 0.04798625007970969 1105 12-13 0.05204721410780877 1105 14-15 0.09282992327747763 1105 16-17 0.05080524497206795 1105 18-19 0.1837124726153334 1105 20-21 0.04204303233709794 1105 22-23 0.04675980989497219 1105 24-25 -0.016660922467174544 1105 26-27 0.0637571027120245 1105 28-29 0.025560177644635473 1105 30-31 0.027418972534739794 1105 32-33 0.132257887089537 1105 34-35 0.22969008543454805 1105 36-37 0.17619289123276616 1105 38-39 0.1442829653308948 1105 40-41 0.18914361699751225 1105 42-43 0.04127331211901719 1105 44-45 0.31341207888595335 1105 46-47 0.2418844662516051 1105 48-49 0.3122446963259833 1105 50-51 0.235217666825406 1105 52-53 0.31292884708163626 1105 54-55 0.17614068213451617 1105 56-57 0.30287639323004356 1105 58-59 0.4144892986592552 1105 60-61 0.44031284289354744 1105 62-63 0.5310863048024999 1105 64-65 0.6260317556299455 1105 66-67 0.6854023015446522 1105 68-69 0.481004489237602 1105 70-71 0.607564376899898 1105 72-73 0.6468491892824879 1105 74-75 0.6705815636021306 1105 76-77 0.8245103417462047 1105 78-79 1.0229985657104343 1105 80-81 1.2291451314405606 1105 82-83 1.2527664656845765 1105 84-85 1.481945317677134 1105 86-87 1.7223385123859387 1105 88-89 1.662518936687185 1105 90-91 1.708999435837928 1105 92-93 1.8183489322732562 1105 94-95 1.9748059791707036 1105 96-97 1.966377074189932 1105 98-99 2.4378164218883533 1105 100-101 2.8531156370609843 1106 1 -0.2990278502411563 1106 2 -0.2715333758274099 1106 3 -0.21232126467427648 1106 4 -0.3846187625994446 1106 5 -0.3591031114260943 1106 6 -0.3100738595730448 1106 7 -0.3202171170791104 1106 8 -0.28525045252024483 1106 9 -0.2302851000121322 1106 10-11 -0.5290665860535739 1106 12-13 -0.6528284737272401 1106 14-15 -0.7332008510980472 1106 16-17 -0.6146431133860446 1106 18-19 -0.23103717511299493 1106 20-21 -0.14033186443599988 1106 22-23 -0.2858169717199175 1106 24-25 -0.649996297250695 1106 26-27 -0.4858289932623805 1106 28-29 -0.15805891010904816 1106 30-31 -0.08243729479024609 1106 32-33 -0.10222150179748724 1106 34-35 -0.22607812928324478 1106 36-37 -0.5526134533999851 1106 38-39 -0.6200504452859121 1106 40-41 -0.353807279806702 1106 42-43 -0.19068913880718696 1106 44-45 -0.09017399570183926 1106 46-47 -0.11804410243420449 1106 48-49 -0.04324541940965787 1106 50-51 -0.15520287677388467 1106 52-53 -0.08288707924339178 1106 54-55 0.10856989606439527 1106 56-57 -0.06046941725241339 1106 58-59 -0.28909201254474937 1106 60-61 -0.10813113460966406 1106 62-63 -0.3125529495626722 1106 64-65 -0.14673952758189301 1106 66-67 0.01469052563367157 1106 68-69 -0.07147706358662376 1106 70-71 -0.1071915652998392 1106 72-73 -0.12472310648864848 1106 74-75 0.08631730468014709 1106 76-77 0.5635747305433121 1106 78-79 0.6461725006688575 1106 80-81 0.6367981879532465 1106 82-83 0.4301693376161424 1106 84-85 0.5343992866208431 1106 86-87 0.7439120233059988 1106 88-89 0.8931687837485462 1106 90-91 1.144539795551168 1106 92-93 1.2001465360967956 1106 94-95 1.3723989392783338 1106 96-97 1.3200550383391523 1106 98-99 1.5535148821974616 1106 100-101 1.8885679713863617 1107 1 -0.12003045930567424 1107 2 -0.062047497491196424 1107 3 -0.13433540867676896 1107 4 -0.016973180306301572 1107 5 -0.031411141493514094 1107 6 0.02263062723967124 1107 7 0.05558929811620317 1107 8 -0.052427354396648695 1107 9 -0.08875139667299692 1107 10-11 -0.15879632617038197 1107 12-13 -0.08726678023251822 1107 14-15 0.02874158806741889 1107 16-17 0.00945463117352574 1107 18-19 -0.014265756739810342 1107 20-21 0.13041298481137886 1107 22-23 0.041382826126813654 1107 24-25 0.012128166320160005 1107 26-27 0.07000407464872183 1107 28-29 0.0483266924282475 1107 30-31 0.03261819887348594 1107 32-33 0.204822419937301 1107 34-35 0.23513203276123562 1107 36-37 0.2298975273940087 1107 38-39 0.38892382869421027 1107 40-41 0.31737192511799606 1107 42-43 0.26158758344681843 1107 44-45 0.3870928903164881 1107 46-47 0.5371062418460539 1107 48-49 0.5829228587663025 1107 50-51 0.289552187746537 1107 52-53 0.44379710288862384 1107 54-55 0.5919533833906669 1107 56-57 0.5171876034178453 1107 58-59 0.2269283209600843 1107 60-61 0.6242656065337542 1107 62-63 0.670944993595171 1107 64-65 0.4696915978708205 1107 66-67 0.6709939553693154 1107 68-69 0.7020098014957732 1107 70-71 0.6893887154376159 1107 72-73 0.5523907473989382 1107 74-75 0.6879282615020728 1107 76-77 0.6662300818158258 1107 78-79 0.768850416434173 1107 80-81 0.9912504837714913 1107 82-83 1.2063058612046156 1107 84-85 1.3000266511005734 1107 86-87 1.355720358188627 1107 88-89 1.3799274570505276 1107 90-91 1.6885021243298084 1107 92-93 1.906143493229063 1107 94-95 2.1527690075290806 1107 96-97 2.1669279080555057 1107 98-99 2.1730687422534096 1107 100-101 2.75553846844414 1108 1 -0.10348641797222413 1108 2 -0.050416332100788 1108 3 -0.0031563373207141865 1108 4 0.016195101026845293 1108 5 0.08004188850645022 1108 6 0.12955101732598706 1108 7 0.13173038360987022 1108 8 0.14793438876382226 1108 9 0.2104009506004374 1108 10-11 0.1965566517476276 1108 12-13 0.22467654667234882 1108 14-15 0.233704140007859 1108 16-17 0.3624790172291412 1108 18-19 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-0.8025225477134192 2316 72-73 -1.2518057416146284 2316 74-75 -1.2561720603243707 2316 76-77 -1.215270254078824 2316 78-79 -1.1688922682578742 2316 80-81 -1.3855355497224657 2316 82-83 -1.2858799901770084 2316 84-85 -1.9727088349270474 2316 86-87 -2.2601368378622055 2316 88-89 -2.403808336799859 2316 90-91 -2.285469428200436 2316 92-93 -2.3784886650215213 2316 94-95 -2.2716309800805696 2316 96-97 -2.2827542020211418 2316 98-99 -2.3228927779651265 2316 100-101 -2.093346851765748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7684.0 3 1474.0 4 1459.0 5 1654.0 6 1874.0 7 2227.0 8 2797.0 9 3798.0 10 4521.0 11 6126.0 12 8181.0 13 10372.0 14 11712.0 15 14681.0 16 18637.0 17 23746.0 18 20997.0 19 15289.0 20 15540.0 21 16599.0 22 18205.0 23 20464.0 24 22587.0 25 25881.0 26 29653.0 27 34735.0 28 40583.0 29 48624.0 30 58422.0 31 71131.0 32 88317.0 33 112289.0 34 147923.0 35 197763.0 36 268483.0 37 324206.0 38 198728.0 39 15376.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18480201246139 19.354408766003086 15.130191853393404 22.330597368142115 2 14.825367656699212 20.47156344169255 40.873166960875935 23.829901940732302 3 19.33393122184286 24.703599393720072 30.36224701186861 25.600222372568453 4 12.25970073016051 16.584235392463242 38.109994488266196 33.04606938911005 5 13.019181099471636 37.88816488141282 34.52965007276494 14.563003946350609 6 30.957706965384507 36.56181045816362 17.651642219780417 14.82884035667145 7 27.50855860245645 33.46840573696195 21.04551698988686 17.977518670694735 8 25.77228185032197 34.59913865153219 20.04141682862582 19.587162669520012 9 25.820963016643795 17.13520076252398 21.30712707353127 35.73670914730096 10-11 24.493321665679808 26.951441252141834 27.307732615108915 21.247504467069444 12-13 25.58818243126758 24.418577232300294 28.083362895944674 21.90987744048745 14-15 22.50470205391793 25.546241251848006 27.3134642354404 24.63559245879366 16-17 21.163619197366874 29.061977448980418 27.685082118855885 22.08932123479682 18-19 21.014392339313748 27.74125170889874 30.262235898360114 20.982120053427398 20-21 22.0096223942405 26.360472271111888 30.300352084175387 21.329553250472227 22-23 21.600019071360947 26.306741883275937 30.398937243171904 21.694301802191216 24-25 21.68668039191082 26.334681320316445 30.45280469400207 21.52583359377067 26-27 21.277453602027336 26.44335348599474 30.75204262160826 21.52715029036966 28-29 21.015256355504786 27.242773339761108 30.444904985105207 21.297065319628903 30-31 21.238533444181183 26.52516884602516 30.352511356183953 21.883786353609704 32-33 21.12920792948241 26.621135103085315 30.4153359989918 21.83432096844048 34-35 21.792684061115065 26.594694156130828 30.192402668773177 21.420219113980927 36-37 21.551004861298306 26.312625203252672 30.499754669080943 21.63661526636808 38-39 21.261599561412357 26.887748647159853 30.324888565367345 21.525763226060445 40-41 21.459551217191947 26.894647278483845 30.086981757377764 21.558819746946444 42-43 21.163751585811145 26.8400844857833 29.763058923935287 22.233105004470268 44-45 21.42962863417232 26.747990289575913 29.726241381107947 22.09613969514382 46-47 21.88356434440005 27.13420089435301 29.314648102296502 21.667586658950434 48-49 21.776645437024257 27.153785126114975 29.111221387406218 21.95834804945455 50-51 21.426097454516494 27.470120868416544 29.29113794048434 21.812643736582622 52-53 21.781233598310823 27.786921307790614 28.44242550177541 21.989419592123156 54-55 21.4464390509725 27.405160127431255 28.851153797786722 22.297247023809526 56-57 21.846487314396224 27.561248951389246 28.57310103565944 22.019162698555085 58-59 22.22203892456647 27.18254482609635 28.651832649240294 21.943583600096886 60-61 21.774175173018875 27.33953604096144 29.259852418335747 21.626436367683937 62-63 21.461587574944783 27.535971138238846 29.27710988514272 21.72533140167365 64-65 21.40649267315323 27.783088668596044 28.910650445896575 21.89976821235415 66-67 21.59021086349005 27.449549646290965 28.556118079363873 22.40412141085511 68-69 21.415960909748755 27.421082116084346 28.958858280553795 22.204098693613105 70-71 21.83638705541014 27.387170124420468 28.6575653020207 22.11887751814869 72-73 21.719151820771142 27.219393060570834 28.894870262231066 22.166584856426958 74-75 21.408964350743773 28.110760474331492 28.453183615765195 22.027091559159544 76-77 21.612353194353087 27.476903643522764 28.45081845466141 22.45992470746274 78-79 21.638291485245205 27.45342678972678 28.552066803264303 22.356214921763712 80-81 21.713591504415877 27.525806761713394 28.412439842722097 22.348161891148628 82-83 21.714105821818606 27.407433016059596 28.73862604808502 22.13983511403678 84-85 21.68457899012522 27.519907479911815 28.495914796038356 22.299598733924608 86-87 21.51551191696247 27.755086326313226 28.407290586567402 22.3221111701569 88-89 21.646209537579825 27.49797000319561 28.15838689905546 22.697433560169106 90-91 21.618867836559982 27.192358194068323 28.440707719362855 22.74806625000884 92-93 21.888915603705083 27.261055612390017 28.468434349386847 22.381594434518057 94-95 21.629343776985117 27.188817826356043 28.46985537483922 22.711983021819623 96-97 21.280738241673042 27.217658707291264 28.76782477499659 22.7337782760391 98-99 21.38545451402195 27.400788905760624 28.19320463955588 23.020551940661544 100-101 21.608598564234818 26.422599330486037 29.018504469318657 22.95029763596049 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2681.0 1 1544.5 2 423.0 3 446.0 4 398.0 5 410.0 6 635.5 7 1319.0 8 2305.0 9 2813.5 10 2686.0 11 2559.5 12 2674.0 13 2884.5 14 3124.0 15 3309.5 16 3478.5 17 3581.5 18 3747.0 19 4015.5 20 4422.5 21 5076.0 22 6037.5 23 7292.0 24 8673.5 25 10692.0 26 13561.0 27 16785.0 28 19058.5 29 21295.5 30 24716.0 31 28481.0 32 32076.0 33 35465.5 34 39193.0 35 42540.5 36 46754.0 37 51090.0 38 53825.5 39 55914.0 40 57812.0 41 60416.5 42 62653.0 43 65281.5 44 68460.0 45 70474.0 46 72169.5 47 78029.0 48 87439.5 49 85491.0 50 73968.0 51 66060.0 52 61004.5 53 57824.5 54 56136.5 55 52338.5 56 46464.5 57 41281.5 58 37145.0 59 32886.5 60 27479.5 61 21888.0 62 16910.0 63 12631.0 64 9391.5 65 7165.0 66 5237.0 67 3618.5 68 2523.5 69 1741.0 70 1245.5 71 851.0 72 587.5 73 459.0 74 314.5 75 215.5 76 150.5 77 93.0 78 70.0 79 48.5 80 30.0 81 22.0 82 16.0 83 13.5 84 13.5 85 12.0 86 7.0 87 4.5 88 6.0 89 4.5 90 2.5 91 1.5 92 0.5 93 2.0 94 2.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3666469915654985 2 0.31018345942650827 3 0.31520244006108517 4 0.30835362273681877 5 0.3091901195092483 6 0.2256450043628535 7 0.21607757252819124 8 0.22669062532839035 9 0.22689974952149772 10-11 0.22632465799045243 12-13 0.22794537048703456 14-15 0.22295253037659607 16-17 0.21610371305232967 18-19 0.20810471266597275 20-21 0.20716365379698956 22-23 0.2152933568040386 24-25 0.2138556279764254 26-27 0.22080900739724552 28-29 0.2276316841973735 30-31 0.23095153076295302 32-33 0.22964450455603197 34-35 0.22854660254221826 36-37 0.21396019007297912 38-39 0.20308573203139582 40-41 0.19738709776921992 42-43 0.20993454935566222 44-45 0.2127054449143349 46-47 0.22002479167309288 48-49 0.2300888934663851 50-51 0.22480850759042398 52-53 0.23994387106656997 54-55 0.22266498461107342 56-57 0.19163618245876723 58-59 0.1712988546790754 60-61 0.1895972215759704 62-63 0.18298366896894977 64-65 0.19155776088635199 66-67 0.19260338185188883 68-69 0.18917897318975563 70-71 0.19072126411392248 72-73 0.20128203586584473 74-75 0.20857524210046433 76-77 0.21176438604535172 78-79 0.20755576165906586 80-81 0.2032948562245032 82-83 0.1914531987897983 84-85 0.18465666251380874 86-87 0.1921067118932588 88-89 0.20227537578310475 90-91 0.19963518284512421 92-93 0.1927079439484425 94-95 0.19346601914845676 96-97 0.20279818626587318 98-99 0.20350398041761056 100-101 0.18002978974130815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1912739.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.97289949096851 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0159610656723 39.20840066259297 2 13.958556608605033 14.788504325332571 3 5.077810590150497 8.069590500786534 4 2.3767877956778967 5.036213640472232 5 1.3011132373621068 3.446187037457577 6 0.7701354343665758 2.4477784175480397 7 0.48301139296379436 1.791055978072464 8 0.3375416504798252 1.4304447939906721 9 0.2567977658928863 1.2242990017934214 >10 1.2945759815667899 12.280623001253765 >50 0.08724465312987989 3.1371991905355863 >100 0.03691179343576036 3.37229478479386 >500 0.0013813452709386452 0.4758914525277087 >1k 0.0018746828677024467 2.075785726209549 >5k 2.960025580582811E-4 1.215731486633114 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9477 0.49546749451963906 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7222 0.37757373065535865 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6561 0.34301595774436555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4908 0.25659538494274436 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4664 0.24383880916319478 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3680 0.19239425765878146 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2937 0.15354943878908728 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2892 0.15119679161662936 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2203 0.11517514935388466 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2000 0.10456209655368556 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.228104827684279E-5 0.0 0.0 0.0 0.0 7 5.228104827684279E-5 0.0 0.0 0.0 0.0 8 1.0456209655368559E-4 0.0 0.0 0.0 0.0 9 1.0456209655368559E-4 0.0 0.0 0.0 0.0 10-11 1.30702620692107E-4 0.0 0.0 0.0 0.0 12-13 2.614052413842139E-4 0.0 0.0 0.0 0.0 14-15 3.1368628966105673E-4 0.0 0.0 0.0 0.0 16-17 3.9210786207632096E-4 0.0 0.0 0.0 0.0 18-19 4.1824838621474234E-4 0.0 0.0 0.0 0.0 20-21 4.1824838621474234E-4 0.0 0.0 0.0 5.228104827684279E-5 22-23 4.1824838621474234E-4 0.0 0.0 1.5684314483052836E-4 5.228104827684279E-5 24-25 4.705294344915851E-4 0.0 0.0 2.3526471724579255E-4 1.0456209655368559E-4 26-27 4.966699586300065E-4 0.0 0.0 5.750915310452707E-4 1.0456209655368559E-4 28-29 9.149183448447487E-4 0.0 0.0 9.671993931215917E-4 1.0456209655368559E-4 30-31 0.0010979020138136987 0.0 0.0 0.00224808507590424 1.0456209655368559E-4 32-33 0.00112404253795212 0.0 0.0 0.004391608055254794 1.0456209655368559E-4 34-35 0.001176323586228963 0.0 0.0 0.007345487282896412 1.0456209655368559E-4 36-37 0.0013593072551979124 0.0 0.0 0.010090242317430657 1.0456209655368559E-4 38-39 0.0013593072551979124 0.0 0.0 0.012678154207134376 1.0456209655368559E-4 40-41 0.0013593072551979124 0.0 0.0 0.016729935448589695 1.0456209655368559E-4 42-43 0.0013593072551979124 0.0 0.0 0.02135680822109028 1.0456209655368559E-4 44-45 0.0014638693517515981 0.0 0.0 0.025382448938407173 1.0456209655368559E-4 46-47 0.0017514151172742334 0.0 0.0 0.030636694290229874 1.0456209655368559E-4 48-49 0.0018298366896894976 0.0 0.0 0.036413750124821 1.0456209655368559E-4 50-51 0.0018298366896894976 0.0 0.0 0.04483099889739269 1.0456209655368559E-4 52-53 0.0018298366896894976 0.0 0.0 0.06901098372543248 1.0456209655368559E-4 54-55 0.0018298366896894976 0.0 0.0 0.10291524353296502 1.0456209655368559E-4 56-57 0.0018821177379663404 0.0 0.0 0.1281669898506801 1.0456209655368559E-4 58-59 0.0018821177379663404 0.0 0.0 0.1677698839203885 1.0456209655368559E-4 60-61 0.0019082582621047619 0.0 0.0 0.21720161506614338 1.0456209655368559E-4 62-63 0.0019343987862431831 0.0 0.0 0.2499556918115854 1.0456209655368559E-4 64-65 0.002038960882796869 0.0 0.0 0.27447550345342464 1.0456209655368559E-4 66-67 0.002038960882796869 0.0 0.0 0.3487145920065414 1.0456209655368559E-4 68-69 0.002038960882796869 0.0 0.0 0.4282079259114808 1.0456209655368559E-4 70-71 0.002169663503488976 0.0 0.0 0.4635499145466266 1.0456209655368559E-4 72-73 0.002300366124181083 0.0 0.0 0.5442979936102103 1.0456209655368559E-4 74-75 0.002300366124181083 0.0 0.0 0.6645966856952255 1.0456209655368559E-4 76-77 0.0023526471724579254 0.0 0.0 0.7761121616697312 1.0456209655368559E-4 78-79 0.0023526471724579254 0.0 0.0 0.8930125856167517 1.0456209655368559E-4 80-81 0.0026140524138421393 0.0 0.0 1.045333419771333 1.0456209655368559E-4 82-83 0.0026663334621189823 0.0 0.0 1.210698375470987 1.0456209655368559E-4 84-85 0.0027447550345342468 0.0 0.0 1.3685348602187752 1.0456209655368559E-4 86-87 0.002849317131087932 0.0 0.0 1.6012378060990025 1.0456209655368559E-4 88-89 0.0028754576552263533 0.0 0.0 1.8872674212216094 1.0456209655368559E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8620 0.0 26.483644 1 TACATGG 8560 0.0 25.993002 2 ACATGGG 8590 0.0 24.739296 3 GAGTACT 8095 0.0 22.766148 12-13 GTATCAA 16445 0.0 22.471441 1 TATACCG 195 4.185913E-7 21.943962 5 AGTACTT 8430 0.0 21.523382 12-13 GTACTTT 8575 0.0 21.26854 14-15 AGAGTAC 12890 0.0 21.11484 10-11 CATGGGA 4790 0.0 21.042439 4 GTAAGCG 275 0.0 20.722027 94-95 GTATAGG 495 0.0 20.177061 1 CATGGGG 3740 0.0 19.831188 4 ACTACGC 170 7.944028E-5 19.563095 8 GGTATCA 12855 0.0 19.534649 1 ATCAACG 18945 0.0 19.372946 3 TCAACGC 18970 0.0 19.323366 4 TATCAAC 18985 0.0 19.30759 2 CAACGCA 19035 0.0 19.257887 5 AACGCAG 19400 0.0 18.978664 6 >>END_MODULE