FastQCFastQC Report
Thu 26 May 2016
SRR937716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1912739
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109130.5705430798451854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90910.47528700988477784No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT89710.4690132840915567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61270.3203259827922158No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33140.173259393989457No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30200.15788876579606523No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24510.12814084932654168No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23410.12238993401608898No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22860.11951447636086261No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22210.11611620822286783No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21070.11015616871930776No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG19540.10215716833295083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT95300.023.27569412-13
GTACATG85250.022.40861
AGTACTT97650.022.30210112-13
GTACTTT99850.022.09613614-15
TACATGG86150.021.8372552
AGAGTAC144550.021.8186910-11
GTATCAA201700.021.1334111
ACATGGG88250.020.8331263
TACTTTT112100.019.27914-15
ACTTTTT118500.018.87909716-17
ATCAACG233000.018.268813
TCAACGC232850.018.2601744
TATCAAC233950.018.2555452
GAGTACA71900.018.1755221
CAACGCA234150.018.1385065
AACGCAG237500.017.9593816
CATGGGG39800.017.7853054
GGTATCA160950.017.715091
CATGGGA49950.017.5000974
AGTACAT68250.017.1234232
Produced by FastQC (version 0.11.5)