##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937716_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1912739 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17168573443632 33.0 31.0 34.0 31.0 34.0 2 32.38406337717797 34.0 31.0 34.0 31.0 34.0 3 32.498645659444385 34.0 31.0 34.0 31.0 34.0 4 36.13407422549548 37.0 35.0 37.0 35.0 37.0 5 35.967693971838294 37.0 35.0 37.0 35.0 37.0 6 36.070926561334296 37.0 35.0 37.0 35.0 37.0 7 36.032747280209165 37.0 35.0 37.0 35.0 37.0 8 36.02779678774783 37.0 35.0 37.0 35.0 37.0 9 37.68055808973415 39.0 37.0 39.0 35.0 39.0 10-11 37.632968742729666 39.0 37.0 39.0 35.0 39.0 12-13 37.55367747507632 39.0 37.0 39.0 35.0 39.0 14-15 38.75140910495368 40.0 38.0 41.0 35.0 41.0 16-17 38.73127175218365 40.0 38.0 41.0 35.0 41.0 18-19 38.73686922261741 40.0 38.0 41.0 35.0 41.0 20-21 38.657458231363506 40.0 38.0 41.0 34.0 41.0 22-23 38.509755905013705 40.0 38.0 41.0 34.0 41.0 24-25 38.45094233975466 40.0 38.0 41.0 34.0 41.0 26-27 38.373521165198184 40.0 38.0 41.0 34.0 41.0 28-29 38.26797383228972 40.0 38.0 41.0 34.0 41.0 30-31 38.32949738568618 40.0 38.0 41.0 34.0 41.0 32-33 38.24114372112453 40.0 38.0 41.0 34.0 41.0 34-35 38.09760401183852 40.0 37.0 41.0 34.0 41.0 36-37 37.932246636890866 40.0 37.0 41.0 33.0 41.0 38-39 37.79144331767168 40.0 37.0 41.0 33.0 41.0 40-41 37.626334539108576 40.0 37.0 41.0 33.0 41.0 42-43 37.40431496403848 40.0 37.0 41.0 33.0 41.0 44-45 37.0788589033841 40.0 36.5 41.0 31.5 41.0 46-47 36.69181446083339 40.0 36.0 41.0 31.0 41.0 48-49 36.33069436028648 39.0 36.0 41.0 30.0 41.0 50-51 36.068421776311354 39.0 35.5 41.0 29.5 41.0 52-53 35.853073785811866 39.0 35.0 40.0 29.0 41.0 54-55 35.64619820059088 39.0 35.0 40.0 28.0 41.0 56-57 35.354478838984306 38.5 35.0 40.0 28.0 41.0 58-59 35.103518044019594 38.0 34.5 40.0 27.0 41.0 60-61 34.74079265388535 38.0 34.0 40.0 26.0 41.0 62-63 34.3798314877252 37.5 34.0 40.0 26.0 41.0 64-65 33.97713174667322 37.0 33.5 39.5 26.0 41.0 66-67 33.54196704307279 36.0 33.0 39.0 24.5 40.5 68-69 33.078181602403674 36.0 32.5 39.0 23.0 40.0 70-71 32.652361613372236 35.0 32.0 38.5 22.5 40.0 72-73 32.20435851415169 35.0 31.5 37.5 21.5 39.5 74-75 31.712080947792668 35.0 31.0 37.0 20.0 39.0 76-77 30.484438545980396 33.5 29.5 35.5 19.5 38.0 78-79 31.05356480941728 34.0 31.0 36.0 20.0 38.5 80-81 30.851926478207428 34.0 31.0 36.0 18.0 37.5 82-83 30.49985465868579 34.0 31.0 35.0 17.5 37.0 84-85 30.096804373205124 34.0 30.0 35.0 13.0 36.5 86-87 29.70222152630338 34.0 30.0 35.0 7.5 36.0 88-89 29.253848015855795 34.0 29.0 35.0 4.5 36.0 90-91 28.73690660356693 34.0 29.0 35.0 2.0 35.0 92-93 28.300385206763703 33.5 29.0 35.0 2.0 35.0 94-95 27.81462342745142 33.0 27.0 35.0 2.0 35.0 96-97 27.243951736227473 33.0 26.5 35.0 2.0 35.0 98-99 26.429723814906268 33.0 25.0 34.0 2.0 35.0 100-101 23.99543769432212 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21649773497523483 1101 2 0.20341674006780153 1101 3 0.12332747737177385 1101 4 0.021418335241122577 1101 5 -0.15099487397117883 1101 6 -0.034626533702912354 1101 7 -0.036658950168074966 1101 8 0.03884026348585934 1101 9 0.14305922193693732 1101 10-11 0.10779097406678062 1101 12-13 0.1476428790672557 1101 14-15 0.20292627973299204 1101 16-17 0.08919613503680779 1101 18-19 0.13430165521690896 1101 20-21 0.23307842149223035 1101 22-23 0.25167820713808453 1101 24-25 0.17893479523984723 1101 26-27 0.1636858099769043 1101 28-29 0.15458526234969128 1101 30-31 0.17964046576057768 1101 32-33 0.1853592698328228 1101 34-35 0.14911680210880007 1101 36-37 0.13344300085514504 1101 38-39 0.1390878631402117 1101 40-41 0.061279269808451886 1101 42-43 0.08177587602645531 1101 44-45 0.2688783231608767 1101 46-47 0.24844262793487104 1101 48-49 0.2900828877073849 1101 50-51 0.29653922689284684 1101 52-53 0.2546563316798611 1101 54-55 0.24176965218047286 1101 56-57 0.30529455311384623 1101 58-59 0.2653098171585526 1101 60-61 0.2560875628653818 1101 62-63 0.289096606389343 1101 64-65 0.14188421648565708 1101 66-67 0.32251187458104624 1101 68-69 0.3115625505515567 1101 70-71 0.1710486799876989 1101 72-73 0.21453559755926932 1101 74-75 0.30057739855631027 1101 76-77 0.21170845423205975 1101 78-79 0.2821974704037764 1101 80-81 0.3124620444191315 1101 82-83 0.2670409466032062 1101 84-85 0.5378110745296389 1101 86-87 0.5378927848872301 1101 88-89 0.639997213241049 1101 90-91 0.8235367458244163 1101 92-93 1.1494906726760163 1101 94-95 1.0783616146795403 1101 96-97 1.2777918956302337 1101 98-99 1.7176701310552325 1101 100-101 2.312108508228068 1102 1 0.1542735757354663 1102 2 0.1607166669991429 1102 3 0.16423082316340754 1102 4 0.06701466788609167 1102 5 -0.029038288751181085 1102 6 0.03930524757443976 1102 7 0.11025415066185928 1102 8 0.09581105309584359 1102 9 0.23980296020665293 1102 10-11 0.19412779125897117 1102 12-13 0.1756065268614222 1102 14-15 0.17345955837062377 1102 16-17 0.22167303128675542 1102 18-19 0.25437920693958205 1102 20-21 0.19600340944181482 1102 22-23 0.11369773910463721 1102 24-25 0.09270677111011594 1102 26-27 0.11089446894151678 1102 28-29 0.13756812295491017 1102 30-31 0.2740887141922741 1102 32-33 0.2811355816648202 1102 34-35 0.2379271839002115 1102 36-37 0.24976303283199286 1102 38-39 0.19518940274811314 1102 40-41 0.24554549453156937 1102 42-43 0.34958083480110247 1102 44-45 0.3551620572189762 1102 46-47 0.4380497820966056 1102 48-49 0.43266341974705114 1102 50-51 0.49253080451483555 1102 52-53 0.3383059383393032 1102 54-55 0.40534823032300693 1102 56-57 0.5275845004159763 1102 58-59 0.4169637343873873 1102 60-61 0.665660717553358 1102 62-63 0.5181475937794104 1102 64-65 0.6266378647745441 1102 66-67 0.6827944388400979 1102 68-69 0.5220347693565159 1102 70-71 0.6558630728939576 1102 72-73 0.5370122442637637 1102 74-75 0.4240513952631879 1102 76-77 0.30489824931146003 1102 78-79 0.5784880428829382 1102 80-81 0.5577051389053587 1102 82-83 0.6149638965982369 1102 84-85 0.48324993085843815 1102 86-87 0.5069650186649106 1102 88-89 0.5379729618008007 1102 90-91 0.7293914543312461 1102 92-93 0.9857977195332097 1102 94-95 0.8653189682368527 1102 96-97 1.0734098993278813 1102 98-99 1.2567990773210873 1102 100-101 1.8579152033137305 1103 1 0.10838100436989606 1103 2 0.1561576425769715 1103 3 0.13770398495407932 1103 4 0.03524490852471729 1103 5 -0.15243470595919462 1103 6 -0.07551489792157895 1103 7 0.014759686435517949 1103 8 0.049072654792318815 1103 9 0.19312571639660803 1103 10-11 0.048154168656274976 1103 12-13 0.07417167879157915 1103 14-15 0.17259672014752425 1103 16-17 0.058341868507277184 1103 18-19 0.13992405040991684 1103 20-21 0.08953752761985356 1103 22-23 0.1544735742941441 1103 24-25 0.14670382019047423 1103 26-27 0.08492986447328121 1103 28-29 0.019113376418751216 1103 30-31 -0.019549108225412226 1103 32-33 -0.018894392650288694 1103 34-35 0.03256587281678236 1103 36-37 0.021572792400085916 1103 38-39 -0.015377576926198344 1103 40-41 -0.10878471733860096 1103 42-43 0.05613551983823584 1103 44-45 -0.06301000051775674 1103 46-47 0.03187261839033084 1103 48-49 0.10468088926024421 1103 50-51 0.004883796864497469 1103 52-53 -0.012020161537179774 1103 54-55 -0.12676024741244873 1103 56-57 0.02024230054242082 1103 58-59 -0.09646333758714576 1103 60-61 -0.11392535265889592 1103 62-63 -0.12178120860888697 1103 64-65 -0.07484982594564116 1103 66-67 0.09726277373244585 1103 68-69 -0.08377499365690255 1103 70-71 0.0024096528073727086 1103 72-73 0.1406111862048931 1103 74-75 0.08580976783014194 1103 76-77 -0.05818502243671375 1103 78-79 -0.049213736798954244 1103 80-81 0.05967392599642096 1103 82-83 0.017842162980318932 1103 84-85 0.07882557552154523 1103 86-87 0.20949233577421822 1103 88-89 0.24292471004411453 1103 90-91 0.4179960035364978 1103 92-93 0.6686765716202991 1103 94-95 0.5833502414389535 1103 96-97 0.7114265471314063 1103 98-99 0.8920837988614245 1103 100-101 1.77204934181918 1104 1 0.12644185259463825 1104 2 0.09908490412497173 1104 3 0.04546399228306797 1104 4 0.006209516203597332 1104 5 -0.06528184192779207 1104 6 -0.0010521075148020032 1104 7 -0.0011751384175084922 1104 8 -0.02078969763690708 1104 9 0.04144471768094604 1104 10-11 -0.02760705077471215 1104 12-13 0.0806160357344794 1104 14-15 -0.007779868313903648 1104 16-17 -0.08029670135331202 1104 18-19 0.06861814264594557 1104 20-21 0.09406198900084206 1104 22-23 0.04909954040813602 1104 24-25 0.021356309844442478 1104 26-27 0.08136160709892692 1104 28-29 -0.03482538248042033 1104 30-31 -0.11718828413700066 1104 32-33 -0.08470069593301588 1104 34-35 -0.18968373034314112 1104 36-37 -0.24974117548978114 1104 38-39 -0.3126257706651998 1104 40-41 -0.25928158225794107 1104 42-43 -0.13864006511548865 1104 44-45 -0.29584523568844645 1104 46-47 -0.032030305981898266 1104 48-49 -0.14870942872273218 1104 50-51 -0.2797815713731495 1104 52-53 -0.25816055043164 1104 54-55 -0.356326066488613 1104 56-57 -0.2605291019547167 1104 58-59 -0.3897799076203867 1104 60-61 -0.28316487317389516 1104 62-63 -0.24811201026988527 1104 64-65 -0.2803491221160712 1104 66-67 -0.10858469345007649 1104 68-69 -0.18222965170496508 1104 70-71 -0.1907263792146736 1104 72-73 -0.19955805882117517 1104 74-75 -0.06199620423866037 1104 76-77 -0.2379905752027902 1104 78-79 -0.07013218594264004 1104 80-81 0.045608565862949035 1104 82-83 0.040101103553848105 1104 84-85 0.03444709491253661 1104 86-87 0.09171892132870951 1104 88-89 0.16087284863952078 1104 90-91 0.14755213284381696 1104 92-93 0.4494259519155399 1104 94-95 0.4705661770566074 1104 96-97 0.6181479874186415 1104 98-99 0.8074949797975997 1104 100-101 1.6752853282112241 1105 1 0.09057042950097127 1105 2 0.06838856967349471 1105 3 0.08027328738845796 1105 4 0.0691898064876284 1105 5 -0.04932717548147991 1105 6 -0.024923526894738757 1105 7 0.03647237806914916 1105 8 0.03556761187405755 1105 9 0.09103746030152138 1105 10-11 0.05546361323168725 1105 12-13 -0.04465054075034658 1105 14-15 0.07189574628589668 1105 16-17 0.0892399081744557 1105 18-19 0.12258473574972584 1105 20-21 0.0736355223311449 1105 22-23 0.13089685779222293 1105 24-25 0.07617172332696498 1105 26-27 0.05451130865395726 1105 28-29 0.04175785205210758 1105 30-31 0.1753216709917993 1105 32-33 0.16279183714011225 1105 34-35 0.0984909042950406 1105 36-37 0.11889499275351767 1105 38-39 0.20174568445942498 1105 40-41 0.05290002305938657 1105 42-43 0.005940482530327529 1105 44-45 0.07183232490808678 1105 46-47 0.08602048490421765 1105 48-49 0.03665882980516244 1105 50-51 0.07385157481892435 1105 52-53 -0.05686640689767586 1105 54-55 -0.05210856421626886 1105 56-57 0.04908283138746583 1105 58-59 0.09257962270364573 1105 60-61 0.14420028809965402 1105 62-63 0.2731133734749065 1105 64-65 0.3033640179111998 1105 66-67 0.38279688995621797 1105 68-69 0.2331786780152143 1105 70-71 0.2772486979149491 1105 72-73 0.3041083252922121 1105 74-75 0.41664448547605915 1105 76-77 0.17283755967632075 1105 78-79 0.31451690795262977 1105 80-81 0.36281485358636445 1105 82-83 0.4568408069936005 1105 84-85 0.5642384387551189 1105 86-87 0.5957269689907747 1105 88-89 0.512258257192066 1105 90-91 0.626706710531888 1105 92-93 0.93126764842361 1105 94-95 0.8771676181711747 1105 96-97 1.0727092886368403 1105 98-99 1.5602953675114541 1105 100-101 1.8309088966827325 1106 1 -0.17401998511270378 1106 2 -0.033133477259447375 1106 3 -0.06304186245641574 1106 4 -0.06662738845287919 1106 5 -0.12501710058455728 1106 6 -0.21579328674728515 1106 7 -0.26360354443545475 1106 8 -0.13281533112913735 1106 9 -0.1331033200476739 1106 10-11 -0.0570164036443046 1106 12-13 -0.22367648709617782 1106 14-15 -0.046116357569474076 1106 16-17 -0.03614389661235862 1106 18-19 -0.05818725808120462 1106 20-21 -0.08277625955837209 1106 22-23 -0.04345684095098079 1106 24-25 -0.1547124044464283 1106 26-27 -0.24585539898752984 1106 28-29 -0.09953772028546126 1106 30-31 -0.19548491083802588 1106 32-33 -0.12755001341503913 1106 34-35 -0.4414792322050687 1106 36-37 -0.2403426422556052 1106 38-39 -0.14930913771324583 1106 40-41 -0.120954370992024 1106 42-43 -0.04765742760794467 1106 44-45 0.27759883841738997 1106 46-47 0.5376746914338497 1106 48-49 0.5162116404203161 1106 50-51 0.46254405513716534 1106 52-53 0.5067214855529016 1106 54-55 0.3114520845954871 1106 56-57 0.2806355340973923 1106 58-59 0.49354644379143764 1106 60-61 0.4341891378931493 1106 62-63 0.4849596415824493 1106 64-65 0.34224088492675975 1106 66-67 0.5140847388072487 1106 68-69 0.2696661977067052 1106 70-71 0.44494301602441055 1106 72-73 0.6187134189048535 1106 74-75 0.40442883639940064 1106 76-77 0.3793123834918788 1106 78-79 0.644525350807335 1106 80-81 0.68569426074923 1106 82-83 0.5466733643328361 1106 84-85 0.6836637608060379 1106 86-87 0.7376874708181163 1106 88-89 0.8017987170335807 1106 90-91 0.9307474209371129 1106 92-93 1.2186145214322828 1106 94-95 1.032635107833901 1106 96-97 1.252432244403959 1106 98-99 1.2527693669699822 1106 100-101 1.8841516187913 1107 1 -0.012268073472128549 1107 2 0.04310275381210715 1107 3 0.015555305380274831 1107 4 -0.022847612522085115 1107 5 -0.2572338830521801 1107 6 -0.17128666224071765 1107 7 -0.0980807154812311 1107 8 -0.03538639583018721 1107 9 -0.06137037949120128 1107 10-11 0.009247157510181125 1107 12-13 -0.028675216432269224 1107 14-15 0.010429987554367415 1107 16-17 0.005879288560123541 1107 18-19 -0.054058080803656594 1107 20-21 -0.020356276555006048 1107 22-23 -0.06509367704109792 1107 24-25 0.06917088855713871 1107 26-27 -0.19509129521872381 1107 28-29 -0.3084994797874643 1107 30-31 -0.18757833081669872 1107 32-33 -0.13993406233151973 1107 34-35 -0.10624662865076573 1107 36-37 -0.08861384276637096 1107 38-39 -0.1397415669776194 1107 40-41 -0.10266461734708798 1107 42-43 -0.0062821849447729505 1107 44-45 -0.1263177128326447 1107 46-47 -0.044763532442239296 1107 48-49 -0.20048219299253134 1107 50-51 4.4346755719004705E-4 1107 52-53 -0.12636242294200883 1107 54-55 -0.33723528836814864 1107 56-57 -0.17762904166660576 1107 58-59 -0.16129368074803807 1107 60-61 0.03686454595881372 1107 62-63 -0.2530421925932629 1107 64-65 -0.04838485819544047 1107 66-67 -0.06703934476544049 1107 68-69 -0.005502195285380651 1107 70-71 0.12372386565419191 1107 72-73 0.06351400153667441 1107 74-75 0.002085842657603365 1107 76-77 0.006738114507943038 1107 78-79 0.11637281925571585 1107 80-81 0.03386890041152668 1107 82-83 0.0339140011732475 1107 84-85 0.25031548696331285 1107 86-87 0.28318324351868185 1107 88-89 0.4039711439632008 1107 90-91 0.5066623830165646 1107 92-93 0.7199454758137662 1107 94-95 0.8064689434277703 1107 96-97 1.0379026230425872 1107 98-99 1.1267990145132778 1107 100-101 1.7342493085850101 1108 1 0.057720359049660885 1108 2 0.0992229499119972 1108 3 0.017001711913913198 1108 4 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-0.38901109750112184 2316 86-87 -0.2691304930560179 2316 88-89 -0.32671394560547995 2316 90-91 -0.3027454744014797 2316 92-93 -0.392119434133555 2316 94-95 -0.34177901587509396 2316 96-97 -0.3913994582135878 2316 98-99 -0.33804207601823677 2316 100-101 -1.0312291105869917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 16.0 5 26.0 6 64.0 7 154.0 8 396.0 9 797.0 10 1474.0 11 2546.0 12 3938.0 13 5656.0 14 7339.0 15 10682.0 16 14853.0 17 21159.0 18 19043.0 19 11117.0 20 9214.0 21 9108.0 22 9666.0 23 10516.0 24 12075.0 25 14082.0 26 17269.0 27 21948.0 28 27222.0 29 34454.0 30 43337.0 31 55086.0 32 71235.0 33 94219.0 34 130246.0 35 188273.0 36 290090.0 37 429531.0 38 320165.0 39 25733.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8591378536617 20.234151321756176 15.458338868763748 21.448371955818374 2 15.411632649967036 21.436506953125285 39.94459248710094 23.207267909806735 3 19.815667479985507 25.052450961683743 30.547711946062687 24.584169612268063 4 12.925600408628673 17.309627711883323 37.628813967823106 32.135957911664896 5 13.079044862593763 38.382760883604064 34.27858784403849 14.259606409763673 6 31.102361587231712 37.323963175320834 17.037609417698913 14.53606581974854 7 27.748480059223972 33.98367471986508 20.562345411475377 17.70549980943558 8 26.051907761592147 35.17976054234268 19.501092412503745 19.267239283561427 9 26.103090907855176 17.343192144877058 20.960413313055255 35.59330363421251 10-11 24.763990277816262 27.478500725922355 26.81743301098582 20.940075985275566 12-13 25.999828510451188 24.81074716637048 27.75715197686146 21.432272346316868 14-15 22.639183913748816 25.98148518956324 27.141000418771196 24.238330477916747 16-17 21.215283423404866 29.471297443090773 27.566123762834344 21.74729537067002 18-19 21.15489881264511 27.947487869489773 30.303873136899494 20.593740180965618 20-21 22.169502566478002 26.426907396622013 30.55904676960462 20.844543267295364 22-23 21.8148971382101 26.497847455726394 30.523963964717566 21.163291441345947 24-25 22.016803207980217 26.49325832710315 30.6619334668249 20.828004998091732 26-27 21.576148359367117 26.58135825298909 30.912157481517035 20.930335906126764 28-29 21.209141126153575 27.465910038524143 30.584151745977472 20.740797089344806 30-31 21.538492907254504 26.683576188785764 30.475958695664673 21.30197220829506 32-33 21.42286532558807 26.829117825275688 30.459461536571375 21.28855531256486 34-35 22.071071902648505 26.75676085446054 30.38705228470795 20.785114958183005 36-37 21.899328659059076 26.4818671026209 30.54671860614543 21.072085632174595 38-39 21.50944274153452 27.11112179968098 30.494437557868586 20.88499790091591 40-41 21.766534796435895 27.041692567569335 30.122667023571957 21.069105612422813 42-43 21.490323041099135 27.112817725575454 29.760396871520534 21.63646236180488 44-45 21.869894428879213 26.873399873166175 29.786761288393244 21.469944409561368 46-47 22.20483296466481 27.49996209624 29.215773819637704 21.07943111945749 48-49 22.15446017465007 27.503464926474546 28.978600844129804 21.363474054745577 50-51 21.784467195994853 27.804211656687084 29.159258006450433 21.252063140867623 52-53 22.326392238144418 28.110481385720004 28.244033358454384 21.319093017681194 54-55 21.942408242839196 27.786514521845373 28.518579900341866 21.752497334973565 56-57 22.237038090403345 27.917321704634034 28.43770111865759 21.40793908630503 58-59 22.744530226026654 27.57124730556548 28.280779552254646 21.403442916153224 60-61 22.160211089960523 27.761315056575935 29.011720888213183 21.066752965250355 62-63 21.852850807140964 27.856283580770818 29.036423683523992 21.254441928564223 64-65 21.818214612657556 28.21315401630855 28.63456540594404 21.334065965089852 66-67 22.044094881737657 27.70963524035428 28.370363128477017 21.875906749431053 68-69 21.933023794673502 27.76006031141729 28.633807330744027 21.67310856316518 70-71 22.353703249633117 27.759537500934524 28.354914078711207 21.531845170721148 72-73 22.267320458944194 27.491977468947653 28.620908875130834 21.619793196977316 74-75 21.88192479003272 28.52901742710778 28.086327535102157 21.502730247757338 76-77 22.082364609076304 27.857773590646712 28.155122052721254 21.90473974755573 78-79 22.143873262374008 27.914315544358114 28.161735605328275 21.7800755879396 80-81 22.19855923887159 28.00481403892533 28.14809025172802 21.64853647047506 82-83 22.241935112843592 27.890623692971744 28.38088999213692 21.486551202047746 84-85 21.989445994436757 28.09800738053681 28.19775990748319 21.714786717543245 86-87 21.975083898012223 28.24831302127473 27.94819366364151 21.82840941707154 88-89 22.039389587392737 28.14278372532792 27.77982254766594 22.038004139613403 90-91 22.15523966278435 27.663567575933122 28.062761554404364 22.11843120687816 92-93 22.307004388346297 27.90632199487682 28.057811044662735 21.728862572114146 94-95 22.137494974484234 27.886188340385175 28.137555620500237 21.838761064630354 96-97 21.711848819938318 27.885064297847222 28.37844055043579 22.02464633177867 98-99 21.856306208808963 28.05685474300138 27.767713649676327 22.319125398513332 100-101 21.643939904930868 27.872139285533148 28.185811996111905 22.298108813424083 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 470.0 1 335.0 2 269.5 3 352.0 4 314.0 5 336.0 6 633.5 7 1402.0 8 2344.5 9 2927.5 10 2936.5 11 2808.5 12 2997.5 13 3273.5 14 3506.0 15 3732.5 16 3864.0 17 3949.5 18 4175.5 19 4386.0 20 4741.5 21 5372.5 22 6267.0 23 7563.5 24 8875.5 25 10846.0 26 13692.0 27 16708.0 28 18947.5 29 21384.5 30 24864.0 31 28257.0 32 31785.0 33 35540.0 34 39750.0 35 43174.0 36 47076.0 37 51226.0 38 54021.5 39 56359.5 40 57990.0 41 60132.0 42 62163.5 43 65511.0 44 69468.0 45 71062.5 46 71838.5 47 78635.0 48 90067.5 49 86372.5 50 73188.0 51 65976.0 52 60969.5 53 57748.0 54 56413.0 55 52583.5 56 46103.5 57 40813.0 58 36828.5 59 32510.5 60 26413.5 61 20451.5 62 15923.5 63 11919.0 64 8771.0 65 6438.0 66 4496.5 67 3281.5 68 2330.0 69 1536.0 70 1084.0 71 725.0 72 522.0 73 435.0 74 296.0 75 169.5 76 119.0 77 83.0 78 55.0 79 33.0 80 19.5 81 12.5 82 8.5 83 4.0 84 2.0 85 1.5 86 1.0 87 1.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04250449224907319 2 1.0456209655368557E-4 3 0.0 4 0.0 5 0.01798468060723392 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00449617015180848 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.2281048276842786E-5 22-23 1.3070262069210696E-4 24-25 4.7052943449158515E-4 26-27 0.0016729935448589691 28-29 0.0022219445517658184 30-31 5.228104827684278E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009436729213970124 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.6140524138421393E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.2281048276842786E-5 74-75 2.6140524138421393E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5684314483052836E-4 84-85 2.8754576552263535E-4 86-87 0.0 88-89 0.0 90-91 0.007057941517373777 92-93 0.003110722372472146 94-95 0.0 96-97 0.0 98-99 2.614052413842139E-4 100-101 0.012338327393334897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1912739.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.92880005059677 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34765752944347 36.845793844460786 2 14.81388726687908 15.089070051739325 3 5.401886223389104 8.25334750101171 4 2.4851283124055783 5.062584116903227 5 1.3748911321631136 3.501077778063692 6 0.8300808888148704 2.536501416736451 7 0.560156225003108 1.9969659066196268 8 0.37477267410664905 1.5269378067203998 9 0.27020274975528985 1.238499163386773 >10 1.4059968931759865 12.897870408577042 >50 0.0897291905074567 3.135429255071271 >100 0.041502648311056496 3.7814519111439333 >500 0.002156839673738276 0.7464246714481462 >1k 0.0015405997669559104 1.5519565523382264 >5k 3.0811995339118206E-4 1.2652604329123476 >10k+ 1.0270665113039403E-4 0.570829182867107 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10913 0.5705430798451854 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9091 0.47528700988477784 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8971 0.4690132840915567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6127 0.3203259827922158 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3314 0.173259393989457 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3020 0.15788876579606523 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2451 0.12814084932654168 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2341 0.12238993401608898 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2286 0.11951447636086261 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2221 0.11611620822286783 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2107 0.11015616871930776 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1954 0.10215716833295083 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.228104827684279E-5 0.0 0.0 5.228104827684279E-5 0.0 3 5.228104827684279E-5 0.0 0.0 5.228104827684279E-5 0.0 4 5.228104827684279E-5 0.0 0.0 5.228104827684279E-5 0.0 5 5.228104827684279E-5 0.0 0.0 5.228104827684279E-5 0.0 6 1.5684314483052836E-4 0.0 0.0 5.228104827684279E-5 0.0 7 1.5684314483052836E-4 0.0 0.0 5.228104827684279E-5 0.0 8 2.0912419310737117E-4 0.0 0.0 5.228104827684279E-5 0.0 9 2.0912419310737117E-4 0.0 0.0 5.228104827684279E-5 0.0 10-11 2.0912419310737117E-4 0.0 0.0 5.228104827684279E-5 0.0 12-13 3.398268137994781E-4 0.0 0.0 5.228104827684279E-5 0.0 14-15 4.966699586300065E-4 0.0 0.0 5.228104827684279E-5 0.0 16-17 5.228104827684278E-4 0.0 0.0 5.228104827684279E-5 0.0 18-19 6.535131034605349E-4 0.0 0.0 5.228104827684279E-5 0.0 20-21 7.319346758757991E-4 0.0 0.0 5.228104827684279E-5 0.0 22-23 7.842157241526418E-4 0.0 0.0 2.3526471724579255E-4 0.0 24-25 8.887778207063274E-4 0.0 0.0 3.398268137994781E-4 0.0 26-27 8.887778207063274E-4 0.0 0.0 6.273725793221135E-4 0.0 28-29 0.0010194804413984344 0.0 0.0 0.0010194804413984344 0.0 30-31 0.0010456209655368557 0.0 0.0 0.002300366124181083 0.0 32-33 0.0010456209655368557 0.0 0.0 0.004391608055254794 0.0 34-35 0.0010979020138136984 0.0 0.0 0.0072670657104811475 0.0 36-37 0.0012024641103673842 0.0 0.0 0.010037961269153815 0.0 38-39 0.0013070262069210697 0.0 0.0 0.012625873158857534 0.0 40-41 0.0013070262069210697 0.0 0.0 0.016991340689973905 0.0 42-43 0.001385447779336334 0.0 0.0 0.021644353986612914 0.0 44-45 0.0016207124965821264 0.0 0.0 0.02585297837289876 0.0 46-47 0.0017514151172742334 0.0 0.0 0.03126406686955199 0.0 48-49 0.0018298366896894976 0.0 0.0 0.03711954427655838 0.0 50-51 0.0018298366896894976 0.0 0.0 0.04577205776637586 0.0 52-53 0.0018298366896894976 0.0 0.0 0.07026572888407671 0.0 54-55 0.0019343987862431831 0.0 0.0 0.10432683183643979 0.0 56-57 0.0019343987862431831 0.0 0.0 0.1298922644438159 0.0 58-59 0.0019343987862431831 0.0 0.0 0.16996568794801592 0.0 60-61 0.0019343987862431831 0.0 0.0 0.22007707272136973 0.0 62-63 0.0019343987862431831 0.0 0.0 0.2530141331357807 0.0 64-65 0.001986679834520026 0.0 0.0 0.27753394477761995 0.0 66-67 0.0021435229793505543 0.0 0.0 0.3520605790962593 0.0 68-69 0.0021958040276273973 0.0 0.0 0.4320767234839672 0.0 70-71 0.002300366124181083 0.0 0.0 0.46741871211911296 0.0 72-73 0.002378787696596347 0.0 0.0 0.5490294284792645 0.0 74-75 0.0024049282207347683 0.0 0.0 0.6706612872953392 0.0 76-77 0.0024049282207347683 0.0 0.0 0.7832223842353818 0.0 78-79 0.0024049282207347683 0.0 0.0 0.9014298343893234 0.0 80-81 0.0026663334621189823 0.0 0.0 1.0552668189439331 0.0 82-83 0.002770895558672668 0.0 0.0 1.2230367028643219 0.0 84-85 0.0028231766069495108 0.0 0.0 1.3826246027293845 0.0 86-87 0.0028754576552263533 0.0 0.0 1.6173403689682702 0.0 88-89 0.0028754576552263533 0.0 0.0 1.9081014189599312 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 9530 0.0 23.275694 12-13 GTACATG 8525 0.0 22.4086 1 AGTACTT 9765 0.0 22.302101 12-13 GTACTTT 9985 0.0 22.096136 14-15 TACATGG 8615 0.0 21.837255 2 AGAGTAC 14455 0.0 21.81869 10-11 GTATCAA 20170 0.0 21.133411 1 ACATGGG 8825 0.0 20.833126 3 TACTTTT 11210 0.0 19.279 14-15 ACTTTTT 11850 0.0 18.879097 16-17 ATCAACG 23300 0.0 18.26881 3 TCAACGC 23285 0.0 18.260174 4 TATCAAC 23395 0.0 18.255545 2 GAGTACA 7190 0.0 18.175522 1 CAACGCA 23415 0.0 18.138506 5 AACGCAG 23750 0.0 17.959381 6 CATGGGG 3980 0.0 17.785305 4 GGTATCA 16095 0.0 17.71509 1 CATGGGA 4995 0.0 17.500097 4 AGTACAT 6825 0.0 17.123423 2 >>END_MODULE