FastQCFastQC Report
Thu 26 May 2016
SRR937715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759360
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29720.3913822166034555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22690.2988042562157606No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.19687631689844076No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9830.12945111672987777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.047.5580861
GTATCAA25900.045.1711
ATCAACG34850.033.5593263
TATCAAC35150.033.27292
TCAACGC35450.033.129814
CAACGCA36100.032.533295
GTACGGA753.055482E-431.7053891
AACGCAG37400.031.6274746
CGCAGAG42750.027.6675918
ACGCAGA43000.027.6190247
GTACATG29950.026.2006151
GCAGAGT46200.025.4986869
TACATGG30650.024.973322
GAGTACT25850.023.70485112-13
ACATGGG30500.022.9138663
AGAGTAC42400.022.46242510-11
GTACTTT27500.022.1083714-15
AGTACTT27450.021.97705512-13
CTGCACG1100.002875913921.5916299
TAGTACC2401.3964382E-719.8119414
Produced by FastQC (version 0.11.5)