##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937715_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759360 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.750603139485882 33.0 31.0 34.0 30.0 34.0 2 31.90324088706279 33.0 31.0 34.0 30.0 34.0 3 31.92617862410451 34.0 31.0 34.0 30.0 34.0 4 35.506905815423515 37.0 35.0 37.0 33.0 37.0 5 35.499304677623265 37.0 35.0 37.0 33.0 37.0 6 35.557485250737464 37.0 35.0 37.0 33.0 37.0 7 35.5027180783818 37.0 35.0 37.0 33.0 37.0 8 35.49078829540666 37.0 35.0 37.0 33.0 37.0 9 37.13814791403287 39.0 37.0 39.0 34.0 39.0 10-11 37.004237779182475 39.0 37.0 39.0 33.0 39.0 12-13 36.895526495996634 39.0 37.0 39.0 33.0 39.0 14-15 38.06011378002529 40.0 38.0 41.0 33.0 41.0 16-17 37.969141118836916 40.0 38.0 41.0 33.0 41.0 18-19 37.900264696586596 40.0 38.0 41.0 33.0 41.0 20-21 37.804931784660766 40.0 38.0 41.0 33.0 41.0 22-23 37.71729021807838 40.0 37.5 41.0 32.5 41.0 24-25 37.75733314896755 40.0 38.0 41.0 33.0 41.0 26-27 37.67133046249473 40.0 37.5 41.0 32.5 41.0 28-29 37.54632519490097 40.0 37.0 41.0 32.0 41.0 30-31 37.308524942056465 40.0 37.0 41.0 32.0 41.0 32-33 37.17853916455962 39.5 36.5 41.0 31.5 41.0 34-35 37.180836493889586 40.0 37.0 41.0 31.0 41.0 36-37 37.03833820585756 40.0 36.5 41.0 31.0 41.0 38-39 36.89052623261694 40.0 36.0 41.0 31.0 41.0 40-41 36.66085124315212 39.5 36.0 41.0 30.0 41.0 42-43 36.39615399810367 39.0 36.0 41.0 30.0 41.0 44-45 36.158179914664984 39.0 35.0 41.0 29.5 41.0 46-47 35.82330449325748 39.0 35.0 40.5 28.5 41.0 48-49 35.50546776232617 39.0 35.0 40.0 27.0 41.0 50-51 34.66220369785082 38.0 34.0 39.5 25.0 40.5 52-53 34.768915929203544 38.0 34.5 40.0 26.0 40.5 54-55 35.04820967656974 39.0 35.0 40.0 26.0 41.0 56-57 34.82305494100295 38.0 34.0 40.0 25.0 41.0 58-59 34.575230457227136 38.0 34.0 40.0 24.5 41.0 60-61 34.2483051517067 38.0 34.0 40.0 23.0 41.0 62-63 33.8728402865571 38.0 33.5 40.0 22.5 41.0 64-65 33.48483788980194 37.0 33.0 40.0 21.5 41.0 66-67 33.02947811314792 36.5 32.5 39.0 19.5 41.0 68-69 32.521184286767806 36.0 32.0 39.0 18.0 40.0 70-71 32.06300239675517 35.5 31.0 39.0 14.5 40.0 72-73 31.5889946797303 35.0 31.0 38.0 10.0 40.0 74-75 31.03259784555415 35.0 30.5 37.0 7.5 39.5 76-77 30.52187960914454 34.5 30.0 37.0 4.5 39.0 78-79 29.92554980509903 34.0 29.0 36.0 2.0 39.0 80-81 29.337791034555416 34.0 29.0 36.0 2.0 37.5 82-83 28.800900100084284 34.0 28.5 35.0 2.0 37.0 84-85 28.256623999157185 34.0 27.5 35.0 2.0 36.5 86-87 27.687272835018963 33.0 26.0 35.0 2.0 36.0 88-89 27.17005899705015 33.0 26.0 35.0 2.0 36.0 90-91 26.580781842604296 32.5 25.0 35.0 2.0 35.0 92-93 25.935300121154654 32.0 23.5 34.5 2.0 35.0 94-95 25.15345883375474 32.0 19.5 34.0 2.0 35.0 96-97 24.29584452697008 31.0 12.5 34.0 2.0 35.0 98-99 23.19513076801517 31.0 2.0 34.0 2.0 35.0 100-101 20.33924225663717 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.01618622367787026 1101 2 0.2646853135848666 1101 3 0.15216765959071665 1101 4 -0.004895130272352333 1101 5 -0.0654589539978474 1101 6 -0.04028365275483736 1101 7 0.1355616555650201 1101 8 0.16063941613816013 1101 9 0.10037112078883581 1101 10-11 0.1523856936610457 1101 12-13 0.1956532093139387 1101 14-15 0.37949307999830495 1101 16-17 0.27507507129899267 1101 18-19 0.3331683814599202 1101 20-21 0.4533309594177837 1101 22-23 0.3223513351907954 1101 24-25 0.27776157455608796 1101 26-27 0.3837610324005567 1101 28-29 0.480593874440153 1101 30-31 0.5881307498678225 1101 32-33 0.17021750789463397 1101 34-35 0.11135913038497591 1101 36-37 0.5290712269892524 1101 38-39 0.2542382261278746 1101 40-41 0.25037363719879835 1101 42-43 0.3517461567825606 1101 44-45 0.5713158848332185 1101 46-47 0.6584200824646373 1101 48-49 0.8406753480200351 1101 50-51 0.46407932509423944 1101 52-53 0.5098366539779917 1101 54-55 0.8271754652402734 1101 56-57 0.8172226914752514 1101 58-59 0.6921741515692688 1101 60-61 0.9795588120414251 1101 62-63 1.0320878190928013 1101 64-65 0.8298748782431389 1101 66-67 0.8715722528955894 1101 68-69 0.8179691897933807 1101 70-71 1.063630529774528 1101 72-73 1.0331744494207875 1101 74-75 1.2397496166924604 1101 76-77 0.9030628707408361 1101 78-79 0.6696429701555076 1101 80-81 0.8773069383225653 1101 82-83 0.6691526290907319 1101 84-85 0.8854183292806539 1101 86-87 1.2371267562517012 1101 88-89 0.9450835079452489 1101 90-91 1.1931256836944044 1101 92-93 1.197372660483314 1101 94-95 1.3654231139359183 1101 96-97 1.8383178950333843 1101 98-99 1.9015325171206747 1101 100-101 1.82712141673505 1102 1 -0.10669902758917615 1102 2 0.1659954350189743 1102 3 0.12376805811405944 1102 4 0.03499089590119553 1102 5 0.04070564384493025 1102 6 -0.06580262352498067 1102 7 0.04051046841787809 1102 8 -0.12392694906902335 1102 9 0.04420965145058631 1102 10-11 -0.05670790638131251 1102 12-13 0.22943383857651867 1102 14-15 0.2940728737095242 1102 16-17 0.19010127854075876 1102 18-19 0.2870085004393488 1102 20-21 0.2583914358168258 1102 22-23 0.2542847028574542 1102 24-25 0.355122271875679 1102 26-27 0.22354068942020433 1102 28-29 0.2701706599739495 1102 30-31 0.3207470941380919 1102 32-33 0.5711125008434692 1102 34-35 0.5238127442890175 1102 36-37 0.5555371720142048 1102 38-39 0.562287627620897 1102 40-41 0.5440054459075938 1102 42-43 0.30962806295629974 1102 44-45 0.04879065224094603 1102 46-47 0.24357316259767003 1102 48-49 0.17459146559404815 1102 50-51 0.011186180369620047 1102 52-53 -0.11141696185128325 1102 54-55 -0.03901719288156613 1102 56-57 -0.12438136499185504 1102 58-59 0.04758670505466256 1102 60-61 -0.051491595386117694 1102 62-63 -0.04415624276496288 1102 64-65 -0.016452979405094936 1102 66-67 0.025791883539525884 1102 68-69 0.2602783630499559 1102 70-71 0.16218925180449162 1102 72-73 0.13151176959194188 1102 74-75 0.26492204542271836 1102 76-77 0.28109932503819834 1102 78-79 0.08915497158018226 1102 80-81 0.05946892747387622 1102 82-83 0.1597947907429642 1102 84-85 0.4977087796409094 1102 86-87 0.5669649885022494 1102 88-89 0.70545690699322 1102 90-91 0.6419012351534903 1102 92-93 0.8015289990741401 1102 94-95 1.0946438275237327 1102 96-97 0.8181474637329345 1102 98-99 0.8146900445432195 1102 100-101 0.8213384964445289 1103 1 0.18382398758704355 1103 2 0.34026359004217355 1103 3 0.27748435060120613 1103 4 0.2108770115414842 1103 5 0.1383200926095114 1103 6 0.22952912111317403 1103 7 0.2575003091302861 1103 8 0.09532803650729704 1103 9 0.22988380170770029 1103 10-11 0.18565883135855898 1103 12-13 0.3268137457632818 1103 14-15 0.2142095851105097 1103 16-17 0.10524237975737094 1103 18-19 0.17295858196450098 1103 20-21 0.23262321808197584 1103 22-23 0.08584622298789668 1103 24-25 0.14503949249527182 1103 26-27 0.13761443832274267 1103 28-29 0.19194163702148614 1103 30-31 0.18684141246243513 1103 32-33 0.25855637391508424 1103 34-35 0.3839895911471629 1103 36-37 0.37844448419280496 1103 38-39 0.35165423993746003 1103 40-41 0.5957275078667053 1103 42-43 0.30048470412022965 1103 44-45 0.3885247548371993 1103 46-47 0.3176180641403761 1103 48-49 0.32629345982457636 1103 50-51 0.20523986154358909 1103 52-53 0.38726860192647194 1103 54-55 0.6730033765402297 1103 56-57 0.48165902131191984 1103 58-59 0.389061683105588 1103 60-61 0.6603395848471649 1103 62-63 0.5454047756722673 1103 64-65 0.3289427311179267 1103 66-67 0.7155159522715024 1103 68-69 0.4534014033108349 1103 70-71 0.6284691966182407 1103 72-73 0.5932501365805258 1103 74-75 0.5464459385620621 1103 76-77 0.46664805199166537 1103 78-79 0.5086909053566764 1103 80-81 0.5827086114071491 1103 82-83 0.43508012875877 1103 84-85 0.27953995987419944 1103 86-87 0.6421951402644446 1103 88-89 1.0156199572879636 1103 90-91 1.3380068415751651 1103 92-93 1.2618262523037913 1103 94-95 1.116320737093531 1103 96-97 1.1748137123219209 1103 98-99 1.2065663771658208 1103 100-101 1.2390507753859588 1104 1 0.1308380540832097 1104 2 0.04707624988872183 1104 3 0.06452718947588565 1104 4 0.13327470975120548 1104 5 0.12143070642024867 1104 6 0.14393768888811564 1104 7 0.04932895363156575 1104 8 0.12375834341266767 1104 9 0.1284835459532303 1104 10-11 0.12862078788029407 1104 12-13 0.008755881313149416 1104 14-15 -0.040066310030155705 1104 16-17 0.12370625443767835 1104 18-19 -0.137496980695353 1104 20-21 -0.06830389189244812 1104 22-23 0.06584462452241979 1104 24-25 -0.0958444589113796 1104 26-27 0.0515338756628978 1104 28-29 0.11287194392685507 1104 30-31 0.05011027435501347 1104 32-33 0.05522677033452794 1104 34-35 0.01815199523922928 1104 36-37 -0.028066065679318797 1104 38-39 -0.09741705162264225 1104 40-41 -0.09886328497216113 1104 42-43 -0.17425154907670048 1104 44-45 0.18062034307249775 1104 46-47 0.20573826874395706 1104 48-49 0.24689927823890656 1104 50-51 0.22826895361519917 1104 52-53 0.3179047911847803 1104 54-55 0.23667345326657596 1104 56-57 0.31128338192574034 1104 58-59 0.25554825599561326 1104 60-61 0.23127788407990124 1104 62-63 0.4443354022707311 1104 64-65 0.37090564671894555 1104 66-67 0.3973429983073089 1104 68-69 0.41600690203206625 1104 70-71 0.47233108920136146 1104 72-73 0.4686609422071264 1104 74-75 0.5528829590224191 1104 76-77 0.32656493178706114 1104 78-79 0.14977626341294226 1104 80-81 0.2579196088496083 1104 82-83 0.28123581929585484 1104 84-85 0.49922825910693547 1104 86-87 0.5890749356608644 1104 88-89 0.42479355063834845 1104 90-91 0.6423386830073881 1104 92-93 0.5741024671375499 1104 94-95 0.4313367217482593 1104 96-97 0.7604275230636119 1104 98-99 0.5071642032527741 1104 100-101 0.5865188060764943 1105 1 0.19354378717880394 1105 2 0.3272890121984169 1105 3 0.3802974884746533 1105 4 0.36394437097737153 1105 5 0.2744232657399621 1105 6 0.2284639884355002 1105 7 0.2373518585828691 1105 8 0.1454931877412946 1105 9 0.3110973849327934 1105 10-11 0.1878354875099788 1105 12-13 0.3855667611650233 1105 14-15 0.3646085459566777 1105 16-17 0.5140269409004361 1105 18-19 0.4770984966054286 1105 20-21 0.3339128822126085 1105 22-23 0.4158759130973735 1105 24-25 0.268776735026357 1105 26-27 0.2795461510709387 1105 28-29 0.2511288702744068 1105 30-31 0.3142008483503531 1105 32-33 0.10178888458496971 1105 34-35 0.5366765460553111 1105 36-37 0.7321798710920504 1105 38-39 0.8553822043093362 1105 40-41 0.821123685265114 1105 42-43 0.7507908833742718 1105 44-45 0.7004843020630389 1105 46-47 0.27628757610920474 1105 48-49 0.26564027741596874 1105 50-51 0.2415323091219861 1105 52-53 0.3824235471853328 1105 54-55 0.3637307842225468 1105 56-57 0.47084439893300356 1105 58-59 0.671639155735015 1105 60-61 0.5649619637671819 1105 62-63 0.5450781844679611 1105 64-65 0.7272782293550719 1105 66-67 0.8762489268493425 1105 68-69 0.6268361778515654 1105 70-71 0.6319113936863374 1105 72-73 0.6474494314404318 1105 74-75 0.8909217225665138 1105 76-77 0.5810310544039865 1105 78-79 0.7797931506712068 1105 80-81 0.7805245327283608 1105 82-83 0.8635826338617605 1105 84-85 0.7584841053557341 1105 86-87 1.3272795715132872 1105 88-89 1.1007489237010262 1105 90-91 0.8241081590737735 1105 92-93 0.8874339717322535 1105 94-95 1.2271594826864032 1105 96-97 1.5096903128043877 1105 98-99 1.368332938680016 1105 100-101 1.085764451392638 1106 1 0.1318409675749166 1106 2 0.2654788720312631 1106 3 0.08631394429435346 1106 4 0.22196251790897747 1106 5 0.42853234245789906 1106 6 0.29051153469716695 1106 7 0.27867677971851634 1106 8 0.370124883080976 1106 9 0.33968368042207686 1106 10-11 0.21390625403897445 1106 12-13 0.19826310901373034 1106 14-15 0.3090246245283339 1106 16-17 0.16442370177070842 1106 18-19 0.41177604709488236 1106 20-21 0.5422351040734767 1106 22-23 0.5418377329939617 1106 24-25 0.5278290619312926 1106 26-27 0.432926748813955 1106 28-29 0.8173570402961943 1106 30-31 0.6756467490300508 1106 32-33 0.7035108918047115 1106 34-35 0.7480769167990715 1106 36-37 0.5119678316761309 1106 38-39 0.6320302253831898 1106 40-41 0.63788032775755 1106 42-43 0.5624010170371463 1106 44-45 0.7686035904951751 1106 46-47 0.7524998106534113 1106 48-49 0.895511167526216 1106 50-51 0.8264830392270284 1106 52-53 0.8113074133273201 1106 54-55 0.5420634129259199 1106 56-57 0.6181174809844521 1106 58-59 0.48685428468230185 1106 60-61 0.5336324896379452 1106 62-63 0.6832095058117531 1106 64-65 0.9709560725369641 1106 66-67 0.9769107369895096 1106 68-69 1.1627040109518347 1106 70-71 0.83792947557518 1106 72-73 1.0536382505890138 1106 74-75 0.989459886409243 1106 76-77 1.2539797681832958 1106 78-79 1.2432391163936671 1106 80-81 0.9312107339056332 1106 82-83 0.9730122088072832 1106 84-85 1.2415957512629205 1106 86-87 1.2183443215864607 1106 88-89 1.2374574345469078 1106 90-91 1.3654477148316815 1106 92-93 1.3094005397507296 1106 94-95 1.41083862672361 1106 96-97 1.7195377875190125 1106 98-99 1.7434735936606636 1106 100-101 1.534344893033019 1107 1 0.6576179958966861 1107 2 0.5465964131342353 1107 3 0.5227106790508032 1107 4 0.5618851025102174 1107 5 0.40427679499306635 1107 6 0.42618776970272165 1107 7 0.4731005715400798 1107 8 0.6067822241302991 1107 9 0.5820858572511085 1107 10-11 0.5409748164886707 1107 12-13 0.6610232362509194 1107 14-15 0.5202996791406846 1107 16-17 0.8324630226613721 1107 18-19 0.6995428811116611 1107 20-21 0.6829184291031822 1107 22-23 0.6588748693906936 1107 24-25 0.7755315108639564 1107 26-27 0.7071727151650862 1107 28-29 0.6886002856110309 1107 30-31 0.6647836823071742 1107 32-33 0.5835093588265181 1107 34-35 0.5462047671804058 1107 36-37 0.514232806956862 1107 38-39 0.30740101448371604 1107 40-41 0.48455759752338423 1107 42-43 0.6379327688479819 1107 44-45 0.8127648598011206 1107 46-47 0.8695292821591849 1107 48-49 0.9112989266953448 1107 50-51 0.5558434040758442 1107 52-53 0.5437260696719335 1107 54-55 0.6838409011411528 1107 56-57 0.6278518925124459 1107 58-59 0.9023833537812891 1107 60-61 1.275869871311194 1107 62-63 1.0031305574347869 1107 64-65 1.0396162495959445 1107 66-67 0.9661894707495264 1107 68-69 0.8696445697223893 1107 70-71 1.1508505655226742 1107 72-73 0.9023601300202806 1107 74-75 1.3633954246762094 1107 76-77 0.9159964009966934 1107 78-79 1.0054741985996252 1107 80-81 1.2795072118882338 1107 82-83 1.2977851364123865 1107 84-85 1.1410553764497493 1107 86-87 0.7965664501266794 1107 88-89 0.523288883594546 1107 90-91 1.2459884728893975 1107 92-93 1.298847901112012 1107 94-95 1.7068892916528995 1107 96-97 1.7510328245021114 1107 98-99 1.808648161552437 1107 100-101 1.9176162684976994 1108 1 0.24723672458267032 1108 2 0.4000788549911327 1108 3 0.39210758832604853 1108 4 0.34745455164843975 1108 5 0.28792145381515155 1108 6 0.4782554215507062 1108 7 0.4559495069912458 1108 8 0.5619882212423519 1108 9 0.5493198736571401 1108 10-11 0.5542125503668274 1108 12-13 0.6498861813490819 1108 14-15 0.7602110435451266 1108 16-17 0.7535627492274699 1108 18-19 0.920651824550788 1108 20-21 0.8807394067061765 1108 22-23 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pass #Quality Count 2 3205.0 3 624.0 4 612.0 5 645.0 6 715.0 7 869.0 8 1061.0 9 1321.0 10 1613.0 11 2083.0 12 2807.0 13 3534.0 14 4105.0 15 5032.0 16 6349.0 17 8102.0 18 6974.0 19 5186.0 20 5165.0 21 5421.0 22 5990.0 23 6581.0 24 7673.0 25 8530.0 26 9721.0 27 11665.0 28 13527.0 29 16109.0 30 19651.0 31 24641.0 32 31022.0 33 40027.0 34 54366.0 35 75643.0 36 109119.0 37 144443.0 38 105006.0 39 10220.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83787710677868 19.46518201511069 15.231019178950705 22.465921699159928 2 14.53133314583226 19.906143829168034 42.424758592368704 23.137764432631 3 18.91130379213687 24.817826647812268 30.152908105938476 26.117961454112383 4 12.173312541180477 16.879143630906718 38.529214867357595 32.41832896055521 5 13.217987047973208 37.66969162344042 34.80645911026126 14.30586221832511 6 30.669741631692897 36.958555258995 18.282892384579966 14.08881072473214 7 27.807845051653846 33.11018167904688 21.124114364123336 17.95785890517594 8 25.413632045598476 35.89745619590458 20.08391386953768 18.604997888959257 9 25.68445301151409 17.438824393983175 21.144389866757436 35.732332727745295 10-11 24.196946056229777 27.34760794196913 27.942760820586578 20.51268518121452 12-13 24.999307343086823 24.62698776042251 28.654226988288162 21.71947790820251 14-15 22.001822045163237 26.09726765790673 27.07043240258622 24.83047789434381 16-17 21.1580937653386 29.415140943564516 27.636721327049234 21.790043964047648 18-19 21.079237541106348 27.793599118666652 30.343726601951992 20.783436738275 20-21 21.869244571096864 26.971160188923193 30.456212564975328 20.703382675004615 22-23 21.55361887498384 26.855677719466282 30.330554961886442 21.26014844366344 24-25 21.1352664971773 27.02572553013802 30.580318564362642 21.25868940832204 26-27 21.095087823914174 27.253447916474272 30.320820481428612 21.33064377818294 28-29 20.99376734956672 27.32438016528926 30.48008823870893 21.201764246435093 30-31 21.240773005884034 27.071105167776423 30.46232444054184 21.2257973857977 32-33 20.94170270457492 27.113701662196977 30.424834984314852 21.519760648913255 34-35 21.4027055288184 27.275702028981684 30.20230441652345 21.119288025676465 36-37 21.36637302158938 27.077809349680365 30.232053484034203 21.323764144696053 38-39 21.200659022915424 27.274064361581445 30.24487793038312 21.280398685120012 40-41 21.37397128265067 27.17067028475279 30.207962999484856 21.247395433111688 42-43 21.192815297145078 27.12628377188324 30.05185551171779 21.629045419253888 44-45 21.281746817072367 27.297908833036484 29.866152120852295 21.554192229038854 46-47 21.349467181915454 27.527373141535534 29.441217176308232 21.68194250024078 48-49 21.419816691448197 27.6055237962048 29.307863110968036 21.66679640137897 50-51 21.475569241811336 27.732281532262533 29.191635727460557 21.600513498465574 52-53 21.49265943056976 27.970043878459965 28.92006202368777 21.617234667282506 54-55 21.481632459216225 27.75862705831757 29.05370820371968 21.706032278746527 56-57 21.413604312413888 28.091105217648817 28.886986448187912 21.608304021749383 58-59 21.576605305212283 27.72602949333435 29.080989601024132 21.61637560042923 60-61 21.572682762113793 27.668688270302212 29.262053119196352 21.496575848387643 62-63 21.440085479105107 27.808080941325453 29.165787252005064 21.58604632756437 64-65 21.42423730781001 27.650230047592416 29.18953072401676 21.736001920580815 66-67 21.338537393520337 27.854011074676244 29.014899897906155 21.79255163389727 68-69 21.319860904804745 27.844565340706716 29.08090844570618 21.75466530878236 70-71 21.409816451859918 27.75191947599509 28.964844948023604 21.873419124121387 72-73 21.442643104516044 27.774784216243454 28.849168687595512 21.933403991644994 74-75 21.32831895062038 27.917742841841164 28.836635948955657 21.9173022585828 76-77 21.32090517483034 27.770628713821466 28.881089776779913 22.027376334568277 78-79 21.31489884048966 27.845463701897867 28.782161257619197 22.05747619999327 80-81 21.35042959400448 27.995401659017055 28.68482303444337 21.969345712535095 82-83 21.5313100187839 27.979965598024524 28.614515839682575 21.874208543509003 84-85 21.473031508526084 27.809096952557827 28.588368436603073 22.129503102313016 86-87 21.345249840403923 27.769123830727914 28.82166655235543 22.063959776512736 88-89 21.48713183284408 27.56464751121344 28.76266025440211 22.185560401540368 90-91 21.34780919667137 27.567193583343393 28.92851041994382 22.15648680004142 92-93 21.434544615181693 27.481566972235044 29.00019785002968 22.083690562553585 94-95 21.260654730027408 27.488214173593946 28.970511031322467 22.280620065056176 96-97 21.2793096534549 27.46019057870077 28.89342208011481 22.367077687729516 98-99 21.154480316996345 27.48596520849052 28.746722137509302 22.612832337003834 100-101 21.51097002563805 26.96466063873561 29.318771694748946 22.205597640877393 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 864.0 1 500.0 2 127.0 3 130.0 4 129.5 5 125.5 6 171.5 7 357.5 8 613.0 9 743.5 10 715.5 11 676.5 12 736.0 13 827.0 14 901.5 15 956.0 16 985.5 17 1050.0 18 1132.5 19 1213.5 20 1375.0 21 1632.0 22 1946.5 23 2368.5 24 3016.5 25 3894.5 26 4945.5 27 6213.5 28 7412.5 29 8736.0 30 10416.5 31 11941.0 32 13676.5 33 15816.5 34 18042.5 35 19886.0 36 21992.0 37 24340.5 38 25587.0 39 26043.0 40 26533.5 41 27554.5 42 28320.0 43 28749.5 44 29358.5 45 30344.0 46 30443.0 47 29784.0 48 29852.5 49 28785.0 50 26918.5 51 25144.5 52 23540.5 53 22222.0 54 20731.0 55 18811.5 56 16626.5 57 14562.5 58 12660.0 59 10821.0 60 8823.0 61 7023.5 62 5356.5 63 3941.5 64 2888.5 65 2092.0 66 1527.5 67 1117.0 68 831.5 69 605.5 70 405.5 71 291.0 72 240.5 73 187.0 74 130.5 75 96.5 76 71.5 77 48.5 78 32.5 79 21.5 80 15.5 81 11.0 82 6.0 83 6.5 84 4.5 85 2.5 86 2.0 87 2.0 88 3.0 89 2.0 90 1.0 91 0.5 92 0.0 93 1.5 94 1.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3002528445006321 2 0.2674620733249052 3 0.2603508217446271 4 0.2664085545722714 5 0.2770754319426886 6 0.18581436999578593 7 0.18831647703329119 8 0.18963337547408343 9 0.18686788874841972 10-11 0.1958886430678466 12-13 0.18555099030762748 14-15 0.18706542351453856 16-17 0.19332069110830172 18-19 0.1866703539823009 20-21 0.18173198482932998 22-23 0.1793615676359039 24-25 0.18397071217867678 26-27 0.17988832701222082 28-29 0.18647281921618206 30-31 0.19246470712178676 32-33 0.19457174462705434 34-35 0.19325484618626212 36-37 0.17146017699115043 38-39 0.1669827222924568 40-41 0.17336967973029918 42-43 0.18285134850400336 44-45 0.1856826801517067 46-47 0.18555099030762748 48-49 0.1847608512431521 50-51 0.1872629582806574 52-53 0.208728402865571 54-55 0.17620101137800254 56-57 0.16718025705857564 58-59 0.16546828908554573 60-61 0.16013485040033712 62-63 0.1685630004214075 64-65 0.1654024441635061 66-67 0.16250526759376316 68-69 0.17356721449641804 70-71 0.167970396123051 72-73 0.16480983986514958 74-75 0.16777286135693215 76-77 0.17073588284871472 78-79 0.174423198482933 80-81 0.1659950484618626 82-83 0.1664559629161399 84-85 0.16013485040033712 86-87 0.15750105351875265 88-89 0.16191266329540666 90-91 0.16731194690265488 92-93 0.15934471133586178 94-95 0.16408554572271386 96-97 0.16434892541087232 98-99 0.1638221660345554 100-101 0.14643910661609777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 759360.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.4016879303447 #Duplication Level Percentage of deduplicated Percentage of total 1 85.14980720222482 61.64989768384499 2 9.527098300937457 13.79555996132382 3 2.4596883711149196 5.342567695540809 4 0.9968319215453192 2.8868925481092025 5 0.4852780817278582 1.756747611634835 6 0.3072189184015153 1.3345900953842726 7 0.20154690734372238 1.02146354021784 8 0.14844957724111082 0.8598399971842006 9 0.1057254719900482 0.6889232366380682 >10 0.5802341421740811 7.3812967783261945 >50 0.030643244658101088 1.474308509290914 >100 0.006565926424519783 0.7051531626895721 >500 3.647736866583556E-4 0.2132626123101148 >1k 5.471605299875334E-4 0.889496567505222 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2972 0.3913822166034555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2269 0.2988042562157606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1495 0.19687631689844076 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 983 0.12945111672987777 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.316898440792246E-4 0.0 0.0 0.0 0.0 3 1.316898440792246E-4 0.0 0.0 0.0 0.0 4 1.316898440792246E-4 0.0 0.0 0.0 0.0 5 1.316898440792246E-4 0.0 0.0 0.0 0.0 6 1.316898440792246E-4 0.0 0.0 0.0 0.0 7 1.316898440792246E-4 0.0 0.0 0.0 0.0 8 1.316898440792246E-4 0.0 0.0 0.0 0.0 9 1.316898440792246E-4 0.0 0.0 0.0 0.0 10-11 1.316898440792246E-4 0.0 0.0 0.0 0.0 12-13 2.633796881584492E-4 0.0 0.0 0.0 0.0 14-15 2.633796881584492E-4 0.0 0.0 0.0 0.0 16-17 2.633796881584492E-4 0.0 0.0 0.0 0.0 18-19 2.633796881584492E-4 0.0 0.0 0.0 0.0 20-21 2.633796881584492E-4 0.0 0.0 0.0 0.0 22-23 2.633796881584492E-4 0.0 0.0 0.0 0.0 24-25 2.633796881584492E-4 0.0 0.0 1.316898440792246E-4 0.0 26-27 2.633796881584492E-4 0.0 0.0 2.633796881584492E-4 0.0 28-29 2.633796881584492E-4 0.0 0.0 9.218289085545723E-4 0.0 30-31 2.633796881584492E-4 0.0 0.0 0.0026337968815844924 0.0 32-33 2.633796881584492E-4 0.0 0.0 0.006057732827644332 0.0 34-35 3.950695322376738E-4 0.0 0.0 0.009415823851664559 0.0 36-37 6.584492203961231E-4 0.0 0.0 0.015605246523388117 0.0 38-39 6.584492203961231E-4 0.0 0.0 0.022321428571428572 0.0 40-41 6.584492203961231E-4 0.0 0.0 0.03022281921618205 0.0 42-43 6.584492203961231E-4 0.0 0.0 0.03753160556257902 0.0 44-45 7.901390644753476E-4 0.0 0.0 0.04293088916982722 0.0 46-47 7.901390644753476E-4 0.0 0.0 0.05129319426885799 0.0 48-49 9.218289085545723E-4 0.0 0.0 0.05728508217446271 0.0 50-51 9.218289085545723E-4 0.0 0.0 0.06393541930046355 0.0 52-53 9.218289085545723E-4 0.0 0.0 0.07822376738305942 0.0 54-55 9.218289085545723E-4 0.0 0.0 0.1016645596291614 0.0 56-57 9.218289085545723E-4 0.0 0.0 0.13945954487989887 0.0 58-59 9.218289085545723E-4 0.0 0.0 0.1857485250737463 0.0 60-61 9.218289085545723E-4 0.0 0.0 0.24435050568900124 0.0 62-63 9.218289085545723E-4 0.0 0.0 0.2825405604719764 0.0 64-65 9.218289085545723E-4 0.0 0.0 0.3323193215339233 0.0 66-67 0.001053518752633797 0.0 0.0 0.4129135061104088 0.0 68-69 0.001053518752633797 0.0 0.0 0.5118125790139065 0.0 70-71 0.001053518752633797 0.0 0.0 0.6288848504003371 0.0 72-73 0.001053518752633797 0.0 0.0 0.7609697640117994 0.0 74-75 0.001053518752633797 0.0 0.0 0.8966103034134008 0.0 76-77 0.001053518752633797 0.0 0.0 1.0289585967130215 0.0 78-79 0.001053518752633797 0.0 0.0 1.1426727770754321 0.0 80-81 0.0011852085967130215 0.0 0.0 1.2261641382216604 0.0 82-83 0.0011852085967130215 0.0 0.0 1.3574589127686472 0.0 84-85 0.0011852085967130215 0.0 0.0 1.6009534344711336 0.0 86-87 0.0011852085967130215 0.0 0.0 1.907790771175727 0.0 88-89 0.0011852085967130215 0.0 0.0 2.2518304888327014 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1370 0.0 47.558086 1 GTATCAA 2590 0.0 45.171 1 ATCAACG 3485 0.0 33.559326 3 TATCAAC 3515 0.0 33.2729 2 TCAACGC 3545 0.0 33.12981 4 CAACGCA 3610 0.0 32.53329 5 GTACGGA 75 3.055482E-4 31.705389 1 AACGCAG 3740 0.0 31.627474 6 CGCAGAG 4275 0.0 27.667591 8 ACGCAGA 4300 0.0 27.619024 7 GTACATG 2995 0.0 26.200615 1 GCAGAGT 4620 0.0 25.498686 9 TACATGG 3065 0.0 24.97332 2 GAGTACT 2585 0.0 23.704851 12-13 ACATGGG 3050 0.0 22.913866 3 AGAGTAC 4240 0.0 22.462425 10-11 GTACTTT 2750 0.0 22.10837 14-15 AGTACTT 2745 0.0 21.977055 12-13 CTGCACG 110 0.0028759139 21.591629 9 TAGTACC 240 1.3964382E-7 19.811941 4 >>END_MODULE