FastQCFastQC Report
Thu 26 May 2016
SRR937715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759360
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39800.524125579435314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34040.4482722292456806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.2853718921196797No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9820.12931942688579856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.050.3460771
GTATCAA37700.036.8104821
GCCGATC902.4126923E-531.6651338
ATCAACG47050.029.491443
TATCAAC48000.029.1057552
TCAACGC47400.028.972924
CAACGCA47800.028.7304695
AACGCAG49150.027.9284536
TAACGCG350.00839439227.1415476-77
GGACCGA1301.0018999E-525.5756826
ACGCAGA54750.025.0718447
CGCAGAG55350.024.8000628
GAGTACT36500.024.39950212-13
GTACATG31700.024.2876631
GTACTTT38000.023.4988614-15
TACATGG33150.023.07892
ACATGGG32000.022.7203083
GCAGAGT60200.022.565359
TGGTATC11700.022.3382722
CATGGGG11200.021.6383884
Produced by FastQC (version 0.11.5)