##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937715_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759360 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30893647281922 33.0 31.0 34.0 31.0 34.0 2 32.593680204382636 34.0 31.0 34.0 31.0 34.0 3 32.69228034134007 34.0 31.0 34.0 31.0 34.0 4 36.282049357353564 37.0 35.0 37.0 35.0 37.0 5 36.21311104087653 37.0 35.0 37.0 35.0 37.0 6 36.25976480193847 37.0 37.0 37.0 35.0 37.0 7 36.21887642225032 37.0 36.0 37.0 35.0 37.0 8 36.19017857142857 37.0 36.0 37.0 35.0 37.0 9 37.925493836915294 39.0 38.0 39.0 35.0 39.0 10-11 37.84585440370839 39.0 38.0 39.0 35.0 39.0 12-13 37.75601822587442 39.0 37.0 39.0 35.0 39.0 14-15 39.02351651390644 40.0 38.0 41.0 36.0 41.0 16-17 38.977209755583644 40.0 38.0 41.0 36.0 41.0 18-19 38.95488898546144 40.0 38.0 41.0 36.0 41.0 20-21 38.8661320058997 40.0 38.0 41.0 35.0 41.0 22-23 38.81382150758533 40.0 38.0 41.0 35.0 41.0 24-25 38.85105417720185 40.0 38.0 41.0 35.0 41.0 26-27 38.73702196586599 40.0 38.0 41.0 35.0 41.0 28-29 38.59296183628318 40.0 38.0 41.0 34.5 41.0 30-31 38.63360132216604 40.0 38.0 41.0 35.0 41.0 32-33 38.55931244732406 40.0 38.0 41.0 35.0 41.0 34-35 38.42590865992415 40.0 38.0 41.0 34.5 41.0 36-37 38.28394042351454 40.0 38.0 41.0 34.0 41.0 38-39 38.1609309155078 40.0 38.0 41.0 34.0 41.0 40-41 37.97795314475348 40.0 38.0 41.0 33.5 41.0 42-43 37.768290402444165 40.0 37.5 41.0 33.0 41.0 44-45 37.45897334597556 40.0 37.0 41.0 33.0 41.0 46-47 37.07906658238517 40.0 37.0 41.0 32.0 41.0 48-49 36.7431593710493 40.0 37.0 41.0 31.0 41.0 50-51 36.48352625895491 40.0 36.0 41.0 30.5 41.0 52-53 36.25864938895913 39.0 36.0 41.0 30.0 41.0 54-55 36.05096462810788 39.0 36.0 40.5 30.0 41.0 56-57 35.79049857774969 39.0 35.0 40.0 29.0 41.0 58-59 35.54468433944374 39.0 35.0 40.0 28.0 41.0 60-61 35.221076301095664 38.0 35.0 40.0 28.0 41.0 62-63 34.86717564791403 38.0 34.0 40.0 27.0 41.0 64-65 34.47373511904762 37.5 34.0 40.0 26.0 41.0 66-67 34.06934523809524 37.0 34.0 39.5 26.0 41.0 68-69 33.62593697324063 36.0 33.0 39.0 25.5 40.0 70-71 33.236834966287404 36.0 33.0 39.0 25.0 40.0 72-73 32.74462376211547 35.0 32.0 38.0 23.5 40.0 74-75 32.25636457016435 35.0 32.0 37.5 22.0 39.0 76-77 31.04394885166456 34.0 30.5 36.0 21.5 38.5 78-79 31.599865676359038 35.0 31.0 36.0 22.0 39.0 80-81 31.395795801727772 35.0 31.0 36.0 20.5 38.0 82-83 31.015998340707966 34.0 31.0 35.5 20.0 37.0 84-85 30.611062605351876 34.0 31.0 35.0 18.0 37.0 86-87 30.22390368204804 34.0 31.0 35.0 16.0 36.0 88-89 29.78926201011378 34.0 30.0 35.0 8.5 36.0 90-91 29.246463469237252 34.0 29.0 35.0 4.5 35.5 92-93 28.810246128318585 34.0 29.0 35.0 2.0 35.0 94-95 28.344492730720606 34.0 29.0 35.0 2.0 35.0 96-97 27.779009955752212 33.0 28.0 35.0 2.0 35.0 98-99 26.902881373788453 33.0 26.5 34.5 2.0 35.0 100-101 24.068061262115464 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24358127750328862 1101 2 0.15921098503641673 1101 3 0.2008961466653858 1101 4 0.08466129722037152 1101 5 0.06292271606390898 1101 6 0.17810556075389883 1101 7 0.09388590330008384 1101 8 0.16325245724333115 1101 9 -0.03547476533687188 1101 10-11 0.14953746608690466 1101 12-13 0.19759236971554373 1101 14-15 0.19308529628220583 1101 16-17 0.13323115039478495 1101 18-19 0.043241031762867976 1101 20-21 0.1344890658002882 1101 22-23 0.05626903177778786 1101 24-25 0.12252703096896767 1101 26-27 -0.1390422239886533 1101 28-29 -0.29165989358783406 1101 30-31 -0.1753853487871666 1101 32-33 -0.097259557243774 1101 34-35 -0.09113133758870617 1101 36-37 -0.18561442554170782 1101 38-39 -0.29757047665142977 1101 40-41 -0.12493613591800568 1101 42-43 -0.1559028380114711 1101 44-45 -0.22519782270416044 1101 46-47 -0.45631411476252026 1101 48-49 -0.28307774781828243 1101 50-51 -0.23278543508146754 1101 52-53 -0.08192890362272465 1101 54-55 -0.23655038215790114 1101 56-57 -0.400098620415811 1101 58-59 -0.38090634393708456 1101 60-61 -0.3089693719906208 1101 62-63 -0.3570647634909676 1101 64-65 -0.23370628093274348 1101 66-67 -0.36506857155488603 1101 68-69 -0.3296274720715857 1101 70-71 -0.11276306153377647 1101 72-73 -0.1868975293581272 1101 74-75 -0.38976547598714717 1101 76-77 -0.2732000325907613 1101 78-79 -0.28752657641938484 1101 80-81 -0.06530001480154013 1101 82-83 0.13690891034773145 1101 84-85 0.08651516277037885 1101 86-87 -0.07452860993064903 1101 88-89 -0.0031174626555845464 1101 90-91 0.09477934685232015 1101 92-93 -0.08180380385362085 1101 94-95 -0.48069977910522965 1101 96-97 -0.6485820598835268 1101 98-99 -0.8310886265652861 1101 100-101 -0.16390974985829843 1102 1 0.17379396768026822 1102 2 0.09207809097721054 1102 3 0.05996905044703027 1102 4 0.020928846927496636 1102 5 0.021693839485820376 1102 6 -0.04233368128164727 1102 7 -0.019707689522647343 1102 8 -0.03788150532050594 1102 9 0.0528742024972928 1102 10-11 0.09272141733999462 1102 12-13 0.19100325787390915 1102 14-15 0.223966042502731 1102 16-17 0.28416103820180183 1102 18-19 0.27537005128954917 1102 20-21 0.29415229432069623 1102 22-23 0.18534511219479555 1102 24-25 0.14923964654314403 1102 26-27 -0.009654496389948974 1102 28-29 0.017644519528779767 1102 30-31 0.06256990878501512 1102 32-33 0.19277567453028155 1102 34-35 0.07236551224789167 1102 36-37 0.048216019139111665 1102 38-39 0.173246592469269 1102 40-41 -0.03957609317782129 1102 42-43 -0.019276232246355107 1102 44-45 0.07781227995891982 1102 46-47 -0.020462896811643816 1102 48-49 0.12111300432233918 1102 50-51 0.1077895840505434 1102 52-53 0.3606064845765573 1102 54-55 0.30355102951960333 1102 56-57 0.24267188693135466 1102 58-59 0.22772255285595833 1102 60-61 0.16915275519993855 1102 62-63 0.1197542212371161 1102 64-65 0.0019856239414650645 1102 66-67 0.032914505642168024 1102 68-69 0.04901904060847784 1102 70-71 -0.139754835973406 1102 72-73 -0.16350834820809013 1102 74-75 0.09901402220072697 1102 76-77 -0.01925811688711221 1102 78-79 -0.0042296620874644475 1102 80-81 -0.1927264107161939 1102 82-83 -0.03948700430666463 1102 84-85 -0.05939708508418917 1102 86-87 0.00399805696062927 1102 88-89 -0.03341462245478155 1102 90-91 5.223185465723645E-4 1102 92-93 -0.2905797403594157 1102 94-95 -0.16964099691171342 1102 96-97 -0.14371001571866415 1102 98-99 -0.12977778316859556 1102 100-101 -0.061494544363682735 1103 1 0.3660377905825669 1103 2 0.15801061323984555 1103 3 0.19145782506134879 1103 4 0.1658404234984232 1103 5 0.03957920258628178 1103 6 0.014299957248390172 1103 7 0.12030736127378816 1103 8 0.11257127280566692 1103 9 0.2146752811377013 1103 10-11 0.20271765464088531 1103 12-13 0.3278752006808858 1103 14-15 0.357366268264677 1103 16-17 0.28805919447923145 1103 18-19 0.15798011023849767 1103 20-21 0.1578033666899401 1103 22-23 0.12385633758047021 1103 24-25 0.09931364629229478 1103 26-27 -0.07268848610524259 1103 28-29 0.03601903351348312 1103 30-31 0.1667577928938826 1103 32-33 0.2359551552778072 1103 34-35 0.28554373876237804 1103 36-37 0.026603075454183056 1103 38-39 -0.03794492891540813 1103 40-41 0.05885486129351847 1103 42-43 0.01546351439857574 1103 44-45 0.03584187985858023 1103 46-47 0.009572221713376905 1103 48-49 -0.3112386826211022 1103 50-51 -0.11917768046721022 1103 52-53 0.06930333645163245 1103 54-55 -0.08157627513359245 1103 56-57 0.0571168888127076 1103 58-59 -0.1773357689802353 1103 60-61 -0.2625718152252503 1103 62-63 -0.3360064710995161 1103 64-65 -0.10879325477419854 1103 66-67 -0.18531953424244563 1103 68-69 -0.24239071732454676 1103 70-71 -0.4573454543102926 1103 72-73 -0.4617800150837965 1103 74-75 -0.35752805731765136 1103 76-77 -0.14797629072381113 1103 78-79 -0.09088336834608768 1103 80-81 -0.10993950490909654 1103 82-83 -0.09493413838417553 1103 84-85 -0.32395622217185505 1103 86-87 -0.4494084399665006 1103 88-89 -0.3138623836488108 1103 90-91 -0.28393397143210564 1103 92-93 -0.4436149843077395 1103 94-95 -0.40648785505795715 1103 96-97 -0.37927253923553295 1103 98-99 -0.40597244013585865 1103 100-101 -0.33034315843617534 1104 1 0.12519380081274534 1104 2 0.09048184086132949 1104 3 0.024590817388720154 1104 4 0.007454873626301151 1104 5 -0.03853780508634941 1104 6 -0.0668829072017374 1104 7 0.06772104413823854 1104 8 0.019009891731499806 1104 9 -0.06679530709759263 1104 10-11 0.11196477740303123 1104 12-13 0.024700648251219093 1104 14-15 -0.053751059101053045 1104 16-17 -0.09625346536731172 1104 18-19 0.009546606402182078 1104 20-21 -0.05404605786248595 1104 22-23 0.02914725829658238 1104 24-25 -0.20498302889951248 1104 26-27 -0.09725849889296256 1104 28-29 -0.2575391506297464 1104 30-31 -0.014257232681565313 1104 32-33 -0.1031896656940603 1104 34-35 -0.08765638911486207 1104 36-37 -0.07892921354837767 1104 38-39 -0.22124735858292155 1104 40-41 -0.21414385993409724 1104 42-43 -0.030102342378924618 1104 44-45 -0.3890126597833614 1104 46-47 -0.4050041849362458 1104 48-49 -0.325971738887084 1104 50-51 -0.44379884415007353 1104 52-53 -0.4553018425764961 1104 54-55 -0.5343991651591722 1104 56-57 -0.5191363081182416 1104 58-59 -0.5545080835582468 1104 60-61 -0.5281840275014602 1104 62-63 -0.5654006526851987 1104 64-65 -0.6817584082268837 1104 66-67 -0.585365889741162 1104 68-69 -0.6267785050483283 1104 70-71 -0.7884595847450768 1104 72-73 -0.8074257951349431 1104 74-75 -0.8471827887754522 1104 76-77 -0.7347026334348072 1104 78-79 -0.8494223709036319 1104 80-81 -1.018558430740299 1104 82-83 -0.9815613762358382 1104 84-85 -0.6380131275427026 1104 86-87 -0.7790567775624098 1104 88-89 -0.8582645575618528 1104 90-91 -0.6860535366494958 1104 92-93 -0.5200935648704039 1104 94-95 -0.35807672973826854 1104 96-97 -0.32559127325088255 1104 98-99 -0.4048615066320238 1104 100-101 -0.5586583758274806 1105 1 0.09786558794756672 1105 2 0.18580055571525378 1105 3 0.008217891864099158 1105 4 0.08468622049229424 1105 5 0.011039584846443518 1105 6 0.06668073290249765 1105 7 -0.011464767135933585 1105 8 -0.04739903708484405 1105 9 0.1433781944796948 1105 10-11 0.10603923563920858 1105 12-13 0.15844859092026553 1105 14-15 0.15360861378332658 1105 16-17 0.05157004992551606 1105 18-19 0.06405730452315339 1105 20-21 -0.04655180124473901 1105 22-23 0.05413097109317988 1105 24-25 -0.016462935633846598 1105 26-27 -0.02026503091874332 1105 28-29 -0.09183400044458523 1105 30-31 0.007976943014497806 1105 32-33 0.16477126216220483 1105 34-35 -0.15861287656514378 1105 36-37 -0.2002924524730787 1105 38-39 -0.30029601446003085 1105 40-41 -0.06648750278144888 1105 42-43 0.07064258287138614 1105 44-45 -0.06165487278569515 1105 46-47 -0.02331143017008941 1105 48-49 0.08118477256539336 1105 50-51 0.1654261421343861 1105 52-53 0.21670174060825076 1105 54-55 0.2139163951206271 1105 56-57 0.1826021365395647 1105 58-59 0.11395946204592633 1105 60-61 -0.0919891468266556 1105 62-63 -0.18467227180466494 1105 64-65 -0.06772441083283809 1105 66-67 0.18935851363286105 1105 68-69 0.029297907652257038 1105 70-71 0.05558829963292311 1105 72-73 0.17343245476218527 1105 74-75 -0.06829443326866169 1105 76-77 -0.4946806885712789 1105 78-79 -0.4065690243119455 1105 80-81 -0.4030050087131407 1105 82-83 -0.32360550598484394 1105 84-85 -0.22626833944778824 1105 86-87 -0.3592841126330448 1105 88-89 -0.13077090099896793 1105 90-91 -0.09898129004409384 1105 92-93 -0.11772892010288061 1105 94-95 -0.06735443512847539 1105 96-97 -0.1250936563259053 1105 98-99 -0.38480895897052037 1105 100-101 -0.29763022468223355 1106 1 0.16501899131027642 1106 2 0.15204336035930766 1106 3 0.17448996575267728 1106 4 0.11936377098174233 1106 5 0.0426113432776134 1106 6 -0.016518720738268655 1106 7 0.08694168510641731 1106 8 -0.05751969141506663 1106 9 0.029338713520964177 1106 10-11 -0.06435329745129792 1106 12-13 -0.24164572108988125 1106 14-15 -0.003640402444844426 1106 16-17 0.023359376407675825 1106 18-19 -0.09823577817871865 1106 20-21 0.06121755710837107 1106 22-23 0.0012365680231667397 1106 24-25 -0.04272395225227399 1106 26-27 0.04023449752120456 1106 28-29 0.013429585363084584 1106 30-31 0.12744912097149808 1106 32-33 0.08585812677569038 1106 34-35 -0.3767728167257971 1106 36-37 0.2249717837622427 1106 38-39 0.262473506500001 1106 40-41 0.05872144710310323 1106 42-43 -0.02158318056807218 1106 44-45 0.2633979744613697 1106 46-47 0.03903856495170288 1106 48-49 0.07879770306720246 1106 50-51 0.27238083984813244 1106 52-53 0.20352407629396652 1106 54-55 0.0019046223800458506 1106 56-57 0.08549166140215192 1106 58-59 -0.03373965012032443 1106 60-61 -0.06483379218097696 1106 62-63 -0.127098041748404 1106 64-65 -0.4434081729064019 1106 66-67 -0.3408440945820459 1106 68-69 0.14492280602294016 1106 70-71 0.3106957828031014 1106 72-73 0.45590342942803375 1106 74-75 0.3542851628157635 1106 76-77 0.3644009075781902 1106 78-79 0.25937133147198566 1106 80-81 -3.154903548185928E-4 1106 82-83 0.14050552201606692 1106 84-85 -0.12523578554359815 1106 86-87 -0.13465404336249875 1106 88-89 -0.03819228055602508 1106 90-91 -0.20785149720530072 1106 92-93 -0.008684723543641582 1106 94-95 -0.18793721830841648 1106 96-97 -0.14339018629435785 1106 98-99 -0.052546121154659176 1106 100-101 0.004914573522587062 1107 1 0.3696984072455862 1107 2 0.14211732655714115 1107 3 0.2531547417462434 1107 4 0.11010458269369394 1107 5 0.18967760626001962 1107 6 0.14910424327506888 1107 7 0.16779478747103127 1107 8 0.20582296753561025 1107 9 0.25708945126672234 1107 10-11 0.16898706203209457 1107 12-13 0.20005320199293664 1107 14-15 0.3693715164606104 1107 16-17 0.36124310001170556 1107 18-19 0.3408360035251832 1107 20-21 0.5419844294496201 1107 22-23 0.41316313836770036 1107 24-25 0.40492484244362004 1107 26-27 0.3306725460399562 1107 28-29 0.11960748748243333 1107 30-31 0.16310810997238434 1107 32-33 0.255740853943891 1107 34-35 0.30294588177572024 1107 36-37 0.3216107290732495 1107 38-39 0.3593500634309805 1107 40-41 0.04851372855789293 1107 42-43 0.04805043764370964 1107 44-45 -0.24277609521248422 1107 46-47 -0.25365104528859206 1107 48-49 -0.07810069880303416 1107 50-51 -0.3009817478190584 1107 52-53 -0.3979916558947494 1107 54-55 -0.43071906979793084 1107 56-57 -0.6600971938463758 1107 58-59 -0.6817027053458631 1107 60-61 -0.49750730615023997 1107 62-63 -0.6891050934480845 1107 64-65 -0.3792415381767853 1107 66-67 -0.5343289372601561 1107 68-69 -0.23273403680847338 1107 70-71 -0.21217816806494483 1107 72-73 -0.42255631073216904 1107 74-75 -0.47981976954157446 1107 76-77 -0.5329489563699958 1107 78-79 -0.47479108756760624 1107 80-81 -0.5193665768531162 1107 82-83 -0.5802107620447892 1107 84-85 -0.5895193098604672 1107 86-87 -0.7568630649391643 1107 88-89 -0.546092866920187 1107 90-91 -0.3558298822127739 1107 92-93 -0.3486226379268125 1107 94-95 -0.12671965037831612 1107 96-97 -0.12443107848766743 1107 98-99 -0.025565704951080903 1107 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 5.0 5 15.0 6 22.0 7 61.0 8 124.0 9 224.0 10 493.0 11 786.0 12 1289.0 13 1948.0 14 2621.0 15 3839.0 16 5458.0 17 8375.0 18 7410.0 19 4074.0 20 3198.0 21 3079.0 22 3192.0 23 3351.0 24 4079.0 25 4762.0 26 5735.0 27 7086.0 28 9050.0 29 11454.0 30 14459.0 31 18407.0 32 24356.0 33 33391.0 34 47586.0 35 71086.0 36 115268.0 37 183826.0 38 147313.0 39 11934.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49847867377234 20.13392939465705 15.80466961536131 21.562922316209303 2 15.183443952802362 20.600373999157185 41.342709650231775 22.87347239780868 3 19.348662031184155 25.174225663716815 30.413374420564686 25.063737884534344 4 12.799462705436158 17.75126422250316 37.96223135271808 31.487041719342606 5 13.383401594262656 37.83231918977417 34.474513797314444 14.309765418648738 6 30.889696586599243 37.50434576485461 17.576116729877793 14.029840918668352 7 27.853587231352716 33.541666666666664 20.73417088074168 17.870575221238937 8 25.66055625790139 36.38300674252002 19.49865676359039 18.4577802359882 9 25.98332806573957 17.45759587020649 20.887457859249896 35.67161820480405 10-11 24.4871997471555 27.75534660766962 27.27434945217025 20.483104193004635 12-13 25.411183235813535 25.05389598502897 28.32564895770367 21.20927182145383 14-15 22.344869363674675 26.340800147492626 26.891790455120102 24.4225400337126 16-17 21.224254635482513 29.75769068689423 27.479324694479562 21.5387299831437 18-19 21.04278603034134 27.872748103666247 30.560340286557103 20.524125579435314 20-21 21.848991255794353 26.872958807416776 30.88660187526338 20.391448061525494 22-23 21.586275239264772 26.808519044060276 30.740659043994427 20.86454667268052 24-25 21.279361247795006 26.97774625982165 31.020319204904684 20.722573287478657 26-27 21.268726493115185 27.184823375720352 30.785722563448836 20.76072756771563 28-29 21.066337791497006 27.297868193574555 30.926469271329914 20.709324743598522 30-31 21.208517699115045 27.075168563000425 30.949417930889172 20.766895806995365 32-33 21.160582595870206 27.125474083438682 30.718565634218287 20.995377686472818 34-35 21.461888959123474 27.1707095448799 30.712112831858406 20.655288664138222 36-37 21.5590102191319 27.02328276443321 30.624736620311843 20.79297039612305 38-39 21.266263695743785 27.389248841129373 30.62802886641382 20.71645859671302 40-41 21.485828743906005 27.28668052054773 30.526605509419845 20.700885226126417 42-43 21.445472116217275 27.217877757180442 30.306249115101068 21.03040101150122 44-45 21.5184497471555 27.342894016013485 30.141698272229245 20.99695796460177 46-47 21.662979351032448 27.696283712600085 29.513669405815424 21.127067530552043 48-49 21.684115571007165 27.873406552886642 29.364201432785503 21.07827644332069 50-51 21.739622840286557 28.01589496418036 29.026614517488415 21.21786767804467 52-53 21.856036662452592 28.25201485461441 28.76705383480826 21.124894648124737 54-55 21.74569489155005 28.13640969790988 28.81533713609957 21.3025582744405 56-57 21.71881584492204 28.304098187947744 28.820717446270542 21.156368520859672 58-59 21.863674673409186 27.998643594605984 28.933048883270125 21.20463284871471 60-61 21.884613358617784 28.061986409608092 29.02351980615255 21.029880425621574 62-63 21.779656552886642 28.032751264222505 29.144081858407077 21.043510324483776 64-65 21.724017593763172 27.99811683522967 29.024968394437423 21.252897176569743 66-67 21.64408185840708 28.209215655288666 28.87885851243152 21.267843973872736 68-69 21.69096344289928 28.129214075010534 28.89452960387695 21.285292878213234 70-71 21.802833965444584 28.194071323219553 28.698180046354828 21.304914664981037 72-73 21.855180678466077 28.12203697850822 28.621602402022756 21.401179941002948 74-75 21.812315634218287 28.372642751790984 28.389630741677202 21.425410872313527 76-77 21.71894753476612 28.23002265065318 28.642014327855037 21.409015486725664 78-79 21.779327328276445 28.258862726506536 28.47733617783397 21.484473767383058 80-81 21.708807416772018 28.528431837336704 28.45936051411715 21.303400231774123 82-83 21.764320494628684 28.552756771349465 28.25250293833932 21.430419795682536 84-85 21.719145069532235 28.40174620733249 28.217117045933414 21.661991677201854 86-87 21.65797513695744 28.37270859671302 28.448035187526337 21.521281078803202 88-89 21.70676622418879 28.24931521281079 28.318979140328697 21.724939422671724 90-91 21.53690230333172 28.308931995869596 28.511961835921863 21.642203864876823 92-93 21.775053796293125 27.914613364658535 28.623710403426106 21.686622435622233 94-95 21.497642751790984 28.19262273493468 28.536925832279813 21.772808680994522 96-97 21.658040981879477 28.17293510324484 28.423540876527603 21.74548303834808 98-99 21.548495423717654 28.215381576348193 28.20748008164878 22.028642918285378 100-101 21.40365805148037 28.491890168887856 28.390336168766677 21.714115610865093 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 136.0 2 101.5 3 131.0 4 118.5 5 101.0 6 192.5 7 436.0 8 742.0 9 933.5 10 911.0 11 887.5 12 949.0 13 1041.0 14 1147.0 15 1231.5 16 1283.5 17 1371.5 18 1475.0 19 1528.5 20 1665.0 21 1885.0 22 2175.0 23 2625.0 24 3189.0 25 3934.0 26 5025.0 27 6270.5 28 7394.0 29 8694.0 30 10360.0 31 11989.5 32 13768.0 33 15750.5 34 17767.0 35 19660.5 36 22075.0 37 24246.5 38 25127.0 39 25722.0 40 26489.5 41 27651.5 42 28313.5 43 28567.5 44 29599.0 45 30256.0 46 29797.0 47 29439.0 48 29523.5 49 28716.5 50 27199.0 51 25422.5 52 23588.5 53 22223.0 54 20774.5 55 18772.5 56 16426.0 57 14401.0 58 12601.5 59 10783.0 60 8724.5 61 6837.5 62 5307.5 63 3851.0 64 2792.5 65 1988.0 66 1403.5 67 1083.5 68 766.0 69 531.0 70 378.5 71 254.5 72 192.0 73 157.0 74 122.5 75 92.0 76 69.5 77 46.0 78 34.0 79 20.0 80 11.0 81 5.5 82 4.5 83 4.5 84 2.0 85 2.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02028023598820059 2 0.0 3 0.0 4 0.0 5 0.03463442899283607 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003555625790139064 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.2922461019806154E-4 24-25 5.926042983565108E-4 26-27 0.001053518752633797 28-29 5.267593763168984E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.901390644753476E-4 42-43 0.012181310577328276 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 6.58449220396123E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.2922461019806154E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.014749262536873156 92-93 0.0018436578171091445 94-95 0.0 96-97 0.0 98-99 0.0013168984407922462 100-101 0.020806995364517487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 759360.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.49173676239636 #Duplication Level Percentage of deduplicated Percentage of total 1 83.58408033086742 58.0840290787963 2 10.299857108813471 14.315099177919253 3 2.8693310381419845 5.981843915602088 4 1.0975482968780272 3.0508214932265725 5 0.5893832610888409 2.0478633215874225 6 0.3484641684913043 1.452922816075503 7 0.2401968994098803 1.1684189793454636 8 0.16398328568562937 0.9116386657838874 9 0.12659072536640228 0.7917308427350556 >10 0.6325062537543652 7.821944137038625 >50 0.03547233009617854 1.6409553422858343 >100 0.011632793728697879 1.2694264136596733 >500 3.814030711036296E-4 0.19759001734948461 >1k 5.721046066554444E-4 1.2657157985948542 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3980 0.524125579435314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3404 0.4482722292456806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2167 0.2853718921196797 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 982 0.12931942688579856 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.316898440792246E-4 0.0 9 0.0 0.0 0.0 1.316898440792246E-4 0.0 10-11 1.316898440792246E-4 0.0 0.0 1.316898440792246E-4 0.0 12-13 1.316898440792246E-4 0.0 0.0 1.316898440792246E-4 0.0 14-15 2.633796881584492E-4 0.0 0.0 1.316898440792246E-4 0.0 16-17 2.633796881584492E-4 0.0 0.0 1.316898440792246E-4 0.0 18-19 2.633796881584492E-4 0.0 0.0 1.316898440792246E-4 0.0 20-21 2.633796881584492E-4 0.0 0.0 1.316898440792246E-4 0.0 22-23 2.633796881584492E-4 0.0 0.0 1.316898440792246E-4 0.0 24-25 3.950695322376738E-4 0.0 0.0 2.633796881584492E-4 0.0 26-27 3.950695322376738E-4 0.0 0.0 3.950695322376738E-4 0.0 28-29 5.267593763168984E-4 0.0 0.0 0.0012510535187526337 0.0 30-31 6.584492203961231E-4 0.0 0.0 0.0030288664138221656 0.0 32-33 6.584492203961231E-4 0.0 0.0 0.006386957437842393 0.0 34-35 7.901390644753476E-4 0.0 0.0 0.009810893383902234 0.0 36-37 9.218289085545723E-4 0.0 0.0 0.016066160977665403 0.0 38-39 9.218289085545723E-4 0.0 0.0 0.02284818794774547 0.0 40-41 9.218289085545723E-4 0.0 0.0 0.03081542351453856 0.0 42-43 9.218289085545723E-4 0.0 0.0 0.0385192793931732 0.0 44-45 9.218289085545723E-4 0.0 0.0 0.04424778761061947 0.0 46-47 9.218289085545723E-4 0.0 0.0 0.05274178255372945 0.0 48-49 9.218289085545723E-4 0.0 0.0 0.0588653603034134 0.0 50-51 9.218289085545723E-4 0.0 0.0 0.06544985250737463 0.0 52-53 9.218289085545723E-4 0.0 0.0 0.07993573535608933 0.0 54-55 9.218289085545723E-4 0.0 0.0 0.10377159713442899 0.0 56-57 9.218289085545723E-4 0.0 0.0 0.14248841129372103 0.0 58-59 9.218289085545723E-4 0.0 0.0 0.18956753055204384 0.0 60-61 9.218289085545723E-4 0.0 0.0 0.24863042562157606 0.0 62-63 9.218289085545723E-4 0.0 0.0 0.28675463548251157 0.0 64-65 9.218289085545723E-4 0.0 0.0 0.3369943109987358 0.0 66-67 9.218289085545723E-4 0.0 0.0 0.4164032869785082 0.0 68-69 9.218289085545723E-4 0.0 0.0 0.5162241887905605 0.0 70-71 9.218289085545723E-4 0.0 0.0 0.6348767383059418 0.0 72-73 9.218289085545723E-4 0.0 0.0 0.7685419300463548 0.0 74-75 9.218289085545723E-4 0.0 0.0 0.9062895069532237 0.0 76-77 9.218289085545723E-4 0.0 0.0 1.0414032869785081 0.0 78-79 9.876738305941847E-4 0.0 0.0 1.1561709860935525 0.0 80-81 0.001053518752633797 0.0 0.0 1.2399257269279393 0.0 82-83 0.001053518752633797 0.0 0.0 1.3720106405394015 0.0 84-85 0.0011193636746734091 0.0 0.0 1.6191924778761062 0.0 86-87 0.0011852085967130215 0.0 0.0 1.9328118415507796 0.0 88-89 0.0011852085967130215 0.0 0.0 2.283304361567636 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2275 0.0 50.346077 1 GTATCAA 3770 0.0 36.810482 1 GCCGATC 90 2.4126923E-5 31.665133 8 ATCAACG 4705 0.0 29.49144 3 TATCAAC 4800 0.0 29.105755 2 TCAACGC 4740 0.0 28.97292 4 CAACGCA 4780 0.0 28.730469 5 AACGCAG 4915 0.0 27.928453 6 TAACGCG 35 0.008394392 27.14154 76-77 GGACCGA 130 1.0018999E-5 25.575682 6 ACGCAGA 5475 0.0 25.071844 7 CGCAGAG 5535 0.0 24.800062 8 GAGTACT 3650 0.0 24.399502 12-13 GTACATG 3170 0.0 24.287663 1 GTACTTT 3800 0.0 23.49886 14-15 TACATGG 3315 0.0 23.0789 2 ACATGGG 3200 0.0 22.720308 3 GCAGAGT 6020 0.0 22.56535 9 TGGTATC 1170 0.0 22.338272 2 CATGGGG 1120 0.0 21.638388 4 >>END_MODULE