FastQCFastQC Report
Thu 26 May 2016
SRR937714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750680
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.3787232908829328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21700.28907124207385304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.18889540150263762No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9610.12801726434699207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18000.045.6901931
GTATCAA33200.042.2409171
ATCAACG38350.036.432283
TCAACGC38650.036.151914
CAACGCA39200.035.5234345
TATCAAC39900.035.259912
AACGCAG40150.034.666716
ACGCAGA45050.030.5817767
CGCAGAG45100.030.339228
GCAGAGT49750.027.3124989
GTACATG31100.024.6101861
TACATGG30500.024.4659582
GAGTACT28000.023.49910212-13
TAGCCCT4700.023.2575194
GTGTAGC4400.022.6890351
GGTATAG1503.0122803E-522.1848321
CAGAGTA47950.022.14434810-11
ACATGGG31200.021.9337223
GTACTTT29600.021.5818514-15
AGAGTAC45500.021.45726610-11
Produced by FastQC (version 0.11.5)