##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937714_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 750680 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.758845313582352 33.0 31.0 34.0 30.0 34.0 2 31.904971492513454 33.0 31.0 34.0 30.0 34.0 3 31.94963899397879 34.0 31.0 34.0 30.0 34.0 4 35.51721905472372 37.0 35.0 37.0 33.0 37.0 5 35.50180103372942 37.0 35.0 37.0 33.0 37.0 6 35.58232269409069 37.0 35.0 37.0 33.0 37.0 7 35.526602547024034 37.0 35.0 37.0 33.0 37.0 8 35.51263387861672 37.0 35.0 37.0 33.0 37.0 9 37.1430343155539 39.0 37.0 39.0 34.0 39.0 10-11 37.026437363457134 39.0 37.0 39.0 33.0 39.0 12-13 36.91798635903447 39.0 37.0 39.0 33.0 39.0 14-15 38.084586641445085 40.0 38.0 41.0 33.0 41.0 16-17 38.02032090904247 40.0 38.0 41.0 33.0 41.0 18-19 37.970580673522676 40.0 38.0 41.0 33.0 41.0 20-21 37.75741128043907 40.0 38.0 41.0 32.5 41.0 22-23 37.64031478126499 40.0 37.5 41.0 32.0 41.0 24-25 37.499770874407204 40.0 37.0 41.0 32.0 41.0 26-27 37.61269049395215 40.0 37.0 41.0 32.0 41.0 28-29 37.563626312143654 40.0 37.0 41.0 32.0 41.0 30-31 37.36497708744072 40.0 37.0 41.0 32.0 41.0 32-33 37.13158070016519 39.5 36.5 41.0 31.0 41.0 34-35 36.890301460009596 39.0 36.0 41.0 31.0 41.0 36-37 36.91563515745724 39.0 36.0 41.0 31.0 41.0 38-39 36.87213193371343 40.0 36.0 41.0 31.0 41.0 40-41 36.68753863164065 39.5 36.0 41.0 30.0 41.0 42-43 36.454425987105026 39.0 36.0 41.0 30.0 41.0 44-45 36.230681515426014 39.0 36.0 41.0 30.0 41.0 46-47 35.88404446635051 39.0 35.0 41.0 28.5 41.0 48-49 35.563359220972984 39.0 35.0 40.0 27.0 41.0 50-51 34.70911440294134 38.0 34.0 39.5 25.0 40.5 52-53 34.83956546064901 38.0 34.5 40.0 26.0 40.5 54-55 35.14162226248202 39.0 35.0 40.0 26.0 41.0 56-57 34.88756061171205 38.5 34.5 40.0 25.5 41.0 58-59 34.66448619917941 38.0 34.0 40.0 25.0 41.0 60-61 34.36239742633346 38.0 34.0 40.0 23.5 41.0 62-63 33.99899957371983 38.0 34.0 40.0 22.5 41.0 64-65 33.63116707518517 37.0 33.0 40.0 22.0 41.0 66-67 33.20491820749187 37.0 32.5 39.5 20.5 41.0 68-69 32.72977766824745 36.0 32.0 39.0 18.5 40.5 70-71 32.29670498747802 36.0 31.5 39.0 17.5 40.0 72-73 31.81301153620717 35.0 31.0 38.0 13.5 40.0 74-75 31.30884797783343 35.0 31.0 37.5 8.0 39.5 76-77 30.819468348697182 35.0 30.5 37.0 7.0 39.0 78-79 30.260902115415355 34.0 30.0 36.0 3.5 39.0 80-81 29.71341650237118 34.0 29.0 36.0 2.0 37.5 82-83 29.165668460595725 34.0 29.0 35.0 2.0 37.0 84-85 28.66642777215325 34.0 28.5 35.0 2.0 37.0 86-87 28.115424015559228 34.0 27.0 35.0 2.0 36.0 88-89 27.6023145681249 33.0 26.0 35.0 2.0 36.0 90-91 27.06097804657111 33.0 25.5 35.0 2.0 35.0 92-93 26.480132679703736 32.5 25.0 35.0 2.0 35.0 94-95 25.8179130921298 32.0 23.5 34.5 2.0 35.0 96-97 25.025355011456277 32.0 19.0 34.0 2.0 35.0 98-99 24.04564528161134 31.5 4.5 34.0 2.0 35.0 100-101 21.466930649544416 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16036790371851595 1101 2 0.03896219004400692 1101 3 0.07063585020038587 1101 4 0.14255826263998728 1101 5 0.09148272656668865 1101 6 0.21265956834813693 1101 7 0.053155641641112084 1101 8 0.26418590561964805 1101 9 0.03984842443779257 1101 10-11 -0.04265890373555692 1101 12-13 0.14729349820085957 1101 14-15 0.1783736728031542 1101 16-17 0.2111495002951429 1101 18-19 0.04765168868394909 1101 20-21 0.2893812239128195 1101 22-23 0.2706946675097299 1101 24-25 0.19599767614219132 1101 26-27 0.28034396415952045 1101 28-29 0.15429158920003516 1101 30-31 0.1485821909991003 1101 32-33 0.12336429090261447 1101 34-35 0.4064525181358718 1101 36-37 0.38994951731029914 1101 38-39 0.4152140968408773 1101 40-41 0.4957766913195485 1101 42-43 0.5582458439962394 1101 44-45 0.6567416666476547 1101 46-47 0.8383870904836215 1101 48-49 1.0266282253075545 1101 50-51 0.7170378898517455 1101 52-53 0.7694279269056139 1101 54-55 0.9999160101812876 1101 56-57 1.1185556295915333 1101 58-59 0.7956637417651251 1101 60-61 1.019038814377062 1101 62-63 1.1434809580839556 1101 64-65 0.9685532936055807 1101 66-67 1.2423539283133707 1101 68-69 1.2533626332676562 1101 70-71 1.2147531888511836 1101 72-73 1.194918275398038 1101 74-75 1.3498918678274414 1101 76-77 1.2350126249410813 1101 78-79 1.1330430977891162 1101 80-81 1.2649905419529865 1101 82-83 1.3718418805210497 1101 84-85 1.6048914800338459 1101 86-87 1.72221966053176 1101 88-89 1.637424523378364 1101 90-91 1.6142286439761655 1101 92-93 1.8233688772925802 1101 94-95 1.879494123306138 1101 96-97 1.7705456353596496 1101 98-99 1.9045149022344496 1101 100-101 2.1046385581716187 1102 1 -0.02605598197734693 1102 2 0.15474631532834238 1102 3 -0.02109610407268292 1102 4 -0.03144031770218447 1102 5 -0.046790626380420974 1102 6 -0.008036893536498724 1102 7 -0.10904356482106436 1102 8 -0.013097023890473736 1102 9 0.007422534630009636 1102 10-11 0.07065590303167113 1102 12-13 0.14543031513193228 1102 14-15 0.17185653840039805 1102 16-17 0.14316099330196863 1102 18-19 0.21330907227153517 1102 20-21 0.0395374711142793 1102 22-23 0.254202221578808 1102 24-25 0.21662843407544585 1102 26-27 0.3317693251306153 1102 28-29 0.2785578400873234 1102 30-31 0.24173380310258352 1102 32-33 0.039251449786462445 1102 34-35 0.07587303190872063 1102 36-37 0.18705863771632636 1102 38-39 0.33894519517151167 1102 40-41 0.21236547790802263 1102 42-43 0.42497900017085755 1102 44-45 0.406488795988281 1102 46-47 0.3301307338384163 1102 48-49 0.21236773412102394 1102 50-51 0.27759958793595985 1102 52-53 0.558145889159313 1102 54-55 0.26445595526146093 1102 56-57 0.18337300182938776 1102 58-59 0.10854416717229043 1102 60-61 0.36866396249374844 1102 62-63 0.4253051901159992 1102 64-65 0.36886710774351883 1102 66-67 0.47477435064744355 1102 68-69 0.3010121380370805 1102 70-71 0.5974193175687148 1102 72-73 0.2865743449066045 1102 74-75 0.18352182732100175 1102 76-77 0.2501250050926416 1102 78-79 0.21432086524992044 1102 80-81 0.24456717095378266 1102 82-83 0.08462747353927114 1102 84-85 0.3675220513188613 1102 86-87 0.4596333946372013 1102 88-89 0.43282741491782417 1102 90-91 0.5819582943689277 1102 92-93 0.7668172883787321 1102 94-95 0.6292662333456462 1102 96-97 0.6973559583270941 1102 98-99 1.2288190483705925 1102 100-101 0.9278393115517893 1103 1 0.016143227874199084 1103 2 0.23023080398784046 1103 3 0.06788363599268621 1103 4 -0.005833124930170186 1103 5 0.031158949382493972 1103 6 0.10833172502428567 1103 7 -0.05353618606703492 1103 8 0.10240221127223492 1103 9 0.2465326822050926 1103 10-11 0.11220802751856951 1103 12-13 0.16071770687168652 1103 14-15 0.3444819662140617 1103 16-17 0.1606174415956616 1103 18-19 0.3088746666986779 1103 20-21 0.24840243056139144 1103 22-23 0.18208796919350334 1103 24-25 0.20355301781687274 1103 26-27 -0.07488145786468436 1103 28-29 0.19339898503224617 1103 30-31 0.1526434642631571 1103 32-33 0.33906326679776555 1103 34-35 0.25765559802037075 1103 36-37 0.35679804279396876 1103 38-39 0.47930824527660576 1103 40-41 0.42504857115214634 1103 42-43 0.39665085745992457 1103 44-45 0.3583821809710628 1103 46-47 0.4464063613901743 1103 48-49 0.5679119791330791 1103 50-51 0.4895216955854309 1103 52-53 0.3644263588464369 1103 54-55 0.5940606609366625 1103 56-57 0.6216830965811795 1103 58-59 0.5341585130988378 1103 60-61 0.6941401975133985 1103 62-63 0.5491075869730082 1103 64-65 0.7214147312454884 1103 66-67 0.7793006467440193 1103 68-69 0.8774182831470796 1103 70-71 0.9308652672325124 1103 72-73 0.835183331469679 1103 74-75 0.980777767190478 1103 76-77 0.7213753765064368 1103 78-79 0.7066202192650408 1103 80-81 0.8894295552652665 1103 82-83 0.7996733087525989 1103 84-85 1.1258169593733136 1103 86-87 1.0647611059578956 1103 88-89 1.1385697472863967 1103 90-91 1.1562850724780738 1103 92-93 1.2112504834177429 1103 94-95 1.1897676144547127 1103 96-97 1.3177820662406852 1103 98-99 1.2113229826055907 1103 100-101 1.1700385214033346 1104 1 0.15473006144632606 1104 2 0.29514295239293276 1104 3 0.3904309551993812 1104 4 0.26650681926710007 1104 5 0.17529589229781095 1104 6 0.053275545249007905 1104 7 0.10386521654211833 1104 8 0.1298737443391218 1104 9 0.36233842837808083 1104 10-11 0.2967265404512318 1104 12-13 0.31408849338854594 1104 14-15 0.27343151752995 1104 16-17 0.41231414621824314 1104 18-19 0.47658826295837997 1104 20-21 0.4918720029918262 1104 22-23 0.3583454158415691 1104 24-25 0.45221073593767613 1104 26-27 0.30820051182085706 1104 28-29 0.5435158691284698 1104 30-31 0.2565383439856461 1104 32-33 0.5349610120658355 1104 34-35 0.6032931819129956 1104 36-37 0.5980355060320335 1104 38-39 0.3679403243053372 1104 40-41 0.6685822413127624 1104 42-43 0.7509944212937256 1104 44-45 0.7348620844474212 1104 46-47 0.7710236602364091 1104 48-49 0.7471480850074457 1104 50-51 0.7169668354693002 1104 52-53 0.6590346086013597 1104 54-55 0.7342197741257124 1104 56-57 0.8703445851567011 1104 58-59 0.6845204767516577 1104 60-61 0.6731312238311702 1104 62-63 0.9306776043171041 1104 64-65 0.8796494043470631 1104 66-67 0.9491822544659101 1104 68-69 0.8673926440550019 1104 70-71 0.7742179555667335 1104 72-73 0.971858544500364 1104 74-75 0.9748015964642924 1104 76-77 1.0426445115641236 1104 78-79 1.1781193520604525 1104 80-81 1.3646501268661986 1104 82-83 1.780097107862776 1104 84-85 1.510772845053129 1104 86-87 1.284220422751499 1104 88-89 1.3943423134578765 1104 90-91 1.6482540039312106 1104 92-93 1.529982102451001 1104 94-95 1.5248403842307496 1104 96-97 1.2347038670098591 1104 98-99 1.3786142879370153 1104 100-101 0.8314441469217719 1105 1 0.17631876832705018 1105 2 0.2794333585454005 1105 3 0.1604009680510181 1105 4 0.335852609846647 1105 5 0.40290854809838095 1105 6 0.3529715938812288 1105 7 0.3282608837051697 1105 8 0.3813476024815472 1105 9 0.5295728789196872 1105 10-11 0.3584786466914096 1105 12-13 0.42487876694477933 1105 14-15 0.368962004464116 1105 16-17 0.562627277325312 1105 18-19 0.5974986891022809 1105 20-21 0.5435631149642504 1105 22-23 0.5723598178325489 1105 24-25 0.5983858445645964 1105 26-27 0.6522214416264518 1105 28-29 0.7870757720272223 1105 30-31 0.6730730943987382 1105 32-33 0.7124828904444911 1105 34-35 0.8787802180811681 1105 36-37 0.893051927565665 1105 38-39 0.9514198534867049 1105 40-41 1.0719971043306629 1105 42-43 0.8127261281132476 1105 44-45 0.9359871124966261 1105 46-47 1.0995124711541848 1105 48-49 1.0360722195620227 1105 50-51 0.905957268141016 1105 52-53 0.9035951030833118 1105 54-55 1.1283610605131784 1105 56-57 1.2042127233015734 1105 58-59 1.37615161442735 1105 60-61 1.2538017527881422 1105 62-63 1.3769669496996997 1105 64-65 1.52934137309515 1105 66-67 1.4767153525259218 1105 68-69 1.5097122621778496 1105 70-71 1.601954027862277 1105 72-73 1.4019262705769506 1105 74-75 1.7272464670299748 1105 76-77 1.378142563419452 1105 78-79 1.1206429643898659 1105 80-81 1.323567733606172 1105 82-83 1.232599096806286 1105 84-85 1.4716640957416054 1105 86-87 1.3905901418514794 1105 88-89 1.4152884385879254 1105 90-91 1.4874810698825378 1105 92-93 1.8353053312713001 1105 94-95 1.56242223690613 1105 96-97 1.709682957603686 1105 98-99 1.9327686299537028 1105 100-101 1.8612171013698635 1106 1 0.06826955017785963 1106 2 0.12604777975608172 1106 3 0.11925767146039945 1106 4 0.06980361142133518 1106 5 0.12752128475931102 1106 6 0.33623595832990105 1106 7 0.11423151394649977 1106 8 0.1652674765065285 1106 9 0.08909974016405187 1106 10-11 -0.09773221499240492 1106 12-13 -0.4444313000710096 1106 14-15 -0.08606784136295431 1106 16-17 -0.08323275856292156 1106 18-19 -0.15760989111524282 1106 20-21 -0.1656231742656189 1106 22-23 -0.32570661202059625 1106 24-25 -0.2459306310643612 1106 26-27 -0.1740044345225229 1106 28-29 0.22946233957400608 1106 30-31 0.27215940926306104 1106 32-33 0.07281174385204281 1106 34-35 0.09999638975876479 1106 36-37 0.056288460322832634 1106 38-39 -0.010174243265041127 1106 40-41 -0.038377524885291336 1106 42-43 0.13120673748081657 1106 44-45 -0.26195663819001425 1106 46-47 0.020274546812444783 1106 48-49 0.05312828577215356 1106 50-51 -0.1272210377878693 1106 52-53 -0.10371071593744574 1106 54-55 -0.1336047358027841 1106 56-57 -0.11391998992213104 1106 58-59 -0.15542453820750524 1106 60-61 -0.34078022252953843 1106 62-63 -0.15054431114917577 1106 64-65 0.008421828655997388 1106 66-67 -0.022039545485576184 1106 68-69 0.15523355708391762 1106 70-71 -0.11948186769564018 1106 72-73 -0.025866969251666205 1106 74-75 -0.1221511233314736 1106 76-77 0.3104401427947039 1106 78-79 0.14231323208679214 1106 80-81 -0.084056685041066 1106 82-83 0.11475385565932683 1106 84-85 0.057347253906179674 1106 86-87 -0.30609019652330005 1106 88-89 0.32146634168969257 1106 90-91 0.7912035586216888 1106 92-93 0.6289205215155604 1106 94-95 0.6083079072470667 1106 96-97 0.7376702868599203 1106 98-99 0.6750691389806924 1106 100-101 0.34832663372420924 1107 1 0.5588594832100462 1107 2 0.5442095096450679 1107 3 0.5635413564185718 1107 4 0.5710963682252199 1107 5 0.6200014988157889 1107 6 0.7021838421922411 1107 7 0.547729698825755 1107 8 0.6756959652587895 1107 9 0.4533527597282756 1107 10-11 0.5806982880553448 1107 12-13 0.6293723361833017 1107 14-15 0.8511695674112545 1107 16-17 0.8193069072744805 1107 18-19 0.8866407233200349 1107 20-21 1.0216029604667014 1107 22-23 0.910452622547119 1107 24-25 0.9891425374675293 1107 26-27 1.001908199168831 1107 28-29 0.9486710521535855 1107 30-31 0.9459656761271447 1107 32-33 0.8436067141261532 1107 34-35 0.8607123665793281 1107 36-37 0.8798121245367696 1107 38-39 1.0083377335254653 1107 40-41 1.2181426293436033 1107 42-43 1.3041185970212794 1107 44-45 1.1867934463653 1107 46-47 1.5246826971931284 1107 48-49 1.4002165765522463 1107 50-51 1.1526589312461013 1107 52-53 1.0702872096388418 1107 54-55 1.0557578298685044 1107 56-57 1.2278751381885584 1107 58-59 1.293660009590674 1107 60-61 1.1337874179638092 1107 62-63 1.4487837559539258 1107 64-65 1.1297680995787402 1107 66-67 1.4314644267351824 1107 68-69 1.4608145836230122 1107 70-71 1.5033788828549106 1107 72-73 1.2511982473226766 1107 74-75 1.6386625618521755 1107 76-77 1.3216427824237158 1107 78-79 1.4084012898700138 1107 80-81 1.3748080941004552 1107 82-83 1.2896762526709544 1107 84-85 1.4959901599032186 1107 86-87 1.2483070556168876 1107 88-89 1.410506845564207 1107 90-91 1.715053042646371 1107 92-93 1.651915808966045 1107 94-95 1.7071342794858708 1107 96-97 1.687795804526342 1107 98-99 1.8702528682834298 1107 100-101 2.472361763638748 1108 1 0.18564936845428548 1108 2 0.38228201903879366 1108 3 0.23124048644125494 1108 4 0.32415990703568553 1108 5 0.2453780940562993 1108 6 0.29242283207071296 1108 7 0.30319667692398866 1108 8 0.2558557431945374 1108 9 0.14849679682925654 1108 10-11 0.36256078424707994 1108 12-13 0.4261039972645122 1108 14-15 0.4763345922919271 1108 16-17 0.33828053714483275 1108 18-19 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sequence quality scores pass #Quality Count 2 3152.0 3 635.0 4 624.0 5 616.0 6 751.0 7 906.0 8 1034.0 9 1342.0 10 1599.0 11 2062.0 12 2699.0 13 3398.0 14 3931.0 15 4833.0 16 5978.0 17 7661.0 18 6814.0 19 4787.0 20 4802.0 21 5165.0 22 5576.0 23 6211.0 24 6877.0 25 7941.0 26 9219.0 27 10816.0 28 12689.0 29 15200.0 30 18316.0 31 23341.0 32 29496.0 33 38668.0 34 52527.0 35 74204.0 36 106754.0 37 144660.0 38 112016.0 39 13375.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76986341999121 19.49648563324734 15.288463979243794 22.445186967517657 2 14.529630312208699 19.87302248682548 42.31991709335278 23.277430107613043 3 18.94643221999207 24.86034825133175 30.08011196177488 26.1131075669013 4 12.133911329417705 16.851363659431033 38.577232189360494 32.43749282179078 5 13.221363483993146 37.65413549900971 34.845540637816804 14.278960379180338 6 30.805000874232043 36.72708878059979 18.2765215975356 14.191388747632566 7 27.67743821142443 33.04866346629938 21.188353225524466 18.08554509675172 8 25.354729188687102 35.88877144656909 20.183930687678163 18.57256867706565 9 25.72448679960492 17.34964362937455 21.128240036304426 35.7976295347161 10-11 24.179308476168927 27.351089594841433 27.880387159378568 20.589214769611072 12-13 24.92425455807373 24.632350978350818 28.680227436533993 21.763167027041455 14-15 22.073345795440364 26.06818298608724 26.994165144496595 24.864306073975797 16-17 21.106002775850104 29.436422356269688 27.669553728714035 21.788021139166176 18-19 21.064225203612814 27.828341302121046 30.283840099295656 20.823593394970487 20-21 21.96391170290776 26.93397601809019 30.334226766565713 20.767885512436337 22-23 21.53361190332883 26.835138788981226 30.242238453602884 21.389010854087065 24-25 21.18550420196105 26.991510664052647 30.53485085273718 21.288134281249125 26-27 21.14735808163309 27.22019367306193 30.253608682190407 21.37883956311457 28-29 20.960863045684803 27.31385305507415 30.480538295783738 21.244745603457307 30-31 21.271339638493195 27.12261963310135 30.381479299140974 21.224561429264472 32-33 20.997403353151313 27.13539334428227 30.287873438992147 21.57932986357427 34-35 21.36001889841966 27.156335041220174 30.231999455469268 21.2516466048909 36-37 21.335260370167227 27.050445927533275 30.16780167000962 21.446492032289875 38-39 21.123730895460547 27.283420725877424 30.239532744014685 21.353315634647345 40-41 21.307582787245085 27.24082992069878 30.125723725382663 21.325863566673473 42-43 21.274954576481996 27.146015174757938 29.95016257715993 21.62886767160014 44-45 21.34540189235717 27.37278635581119 29.7636555322755 21.518156219556136 46-47 21.425411452268868 27.56271456739543 29.36573127237423 21.64614270796147 48-49 21.46498889553259 27.58779469548134 29.313725228495773 21.633491180490307 50-51 21.502108691010037 27.740831197950033 29.15245302156737 21.60460708947256 52-53 21.527168755030697 27.979272536511328 28.812682623396352 21.680876085061623 54-55 21.450505045113093 27.830861599356233 28.974602950066657 21.744030405464017 56-57 21.439546531433827 28.036684197920138 28.90341709327236 21.620352177373675 58-59 21.595508961460684 27.7160434932583 29.002600067920685 21.685847477360326 60-61 21.580971924609262 27.6663081968613 29.224184169866657 21.528535708662783 62-63 21.507893671119167 27.704807456872192 29.1954079736653 21.59189089834334 64-65 21.430329927817656 27.70950659942369 29.093307304367432 21.766856168391218 66-67 21.28670233241734 27.94577685088634 29.00614243483795 21.761378381858368 68-69 21.362739917590044 27.834243365571425 29.04297166890839 21.76004504793014 70-71 21.386853229714873 27.720918688685593 28.979698858844756 21.912529222754785 72-73 21.42181865490658 27.742576833649686 28.880111818688263 21.95549269275547 74-75 21.319966984477766 27.85934907108813 28.82626081862214 21.99442312581196 76-77 21.31527292767253 27.76747338910158 28.897494165417907 22.019759517807984 78-79 21.416661606938522 27.788516263078282 28.796547552881947 21.99827457710125 80-81 21.362657715631155 28.007280389052465 28.711166977244112 21.918894918072272 82-83 21.428218825714314 28.04338196838544 28.61040548184991 21.917993724050337 84-85 21.45457795946031 27.798694394005903 28.553293566561756 22.193434079972036 86-87 21.373622121832312 27.768497789257683 28.834643946408512 22.02323614250149 88-89 21.44501150805564 27.51159144734648 28.754127889522668 22.28926915507522 90-91 21.286580451641484 27.57817795592466 28.93291560143253 22.202325991001327 92-93 21.43382752733767 27.483506667049156 28.94607452337954 22.136591282233635 94-95 21.257553741812472 27.456859111123567 28.99909668656091 22.286490460503053 96-97 21.28410203858337 27.420801076000544 28.936128479588813 22.358968405827266 98-99 21.208485903088402 27.48690924812913 28.71865425469452 22.585950594087944 100-101 21.406036621610713 27.150654024047483 29.1986862812727 22.2446230730691 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 860.0 1 491.0 2 137.5 3 147.0 4 130.5 5 133.5 6 189.0 7 366.5 8 596.5 9 709.0 10 688.5 11 677.5 12 753.0 13 825.0 14 886.0 15 976.0 16 1034.0 17 1028.0 18 1122.0 19 1265.0 20 1381.5 21 1633.5 22 1933.0 23 2295.0 24 2822.0 25 3685.5 26 4820.5 27 6059.0 28 7224.0 29 8583.5 30 10326.5 31 11874.5 32 13522.0 33 15549.0 34 17730.5 35 19535.0 36 21791.5 37 24018.0 38 24858.5 39 25433.5 40 26200.5 41 27174.0 42 27903.5 43 28525.0 44 29506.0 45 29969.5 46 29674.5 47 29513.0 48 29495.0 49 28622.0 50 26849.5 51 24918.5 52 23250.0 53 22031.0 54 20780.0 55 18837.5 56 16383.5 57 14268.0 58 12684.0 59 10770.0 60 8744.0 61 7007.5 62 5350.0 63 3978.0 64 2888.0 65 2042.0 66 1485.5 67 1156.5 68 861.5 69 563.0 70 398.0 71 304.0 72 238.0 73 177.0 74 133.0 75 104.0 76 72.0 77 40.5 78 25.5 79 24.0 80 16.0 81 10.5 82 8.5 83 5.0 84 2.5 85 4.5 86 4.5 87 1.5 88 2.0 89 1.0 90 1.0 91 1.5 92 2.5 93 2.5 94 1.5 95 2.5 96 1.5 97 0.0 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.290536580167315 2 0.2512388767517451 3 0.24684286247135928 4 0.2512388767517451 5 0.2540363403847179 6 0.1932914157830234 7 0.18529866254595834 8 0.18330047423669205 9 0.19449032876858316 10-11 0.1970879735706293 12-13 0.19449032876858316 14-15 0.19655512335482495 16-17 0.1811690733734747 18-19 0.1868306069163958 20-21 0.17630681515426014 22-23 0.18343368679064315 24-25 0.184765812330154 26-27 0.18330047423669205 28-29 0.1900277082112218 30-31 0.18643096925454256 32-33 0.19242553418234135 34-35 0.18796291362498002 36-37 0.1789044599563063 38-39 0.17097831299621677 40-41 0.1677146054244152 42-43 0.17897106623328182 44-45 0.17983694783396387 46-47 0.1801699792188416 48-49 0.19076037725795278 50-51 0.18649757553151808 52-53 0.20368199499120795 54-55 0.1786380348484041 56-57 0.1673815740395375 58-59 0.17004582511855917 60-61 0.15958863963339906 62-63 0.16658229871583097 64-65 0.16764799914743966 66-67 0.16271913465124954 68-69 0.16731496776256197 70-71 0.1683806681941706 72-73 0.16684872382373314 74-75 0.17790536580167315 76-77 0.16904673096392603 78-79 0.17297650130548303 80-81 0.16911333724090158 82-83 0.15346086215164917 84-85 0.1522619491660894 86-87 0.15426013747535566 88-89 0.16052112751105663 90-91 0.16465071668354025 92-93 0.16125379655778763 94-95 0.16211967815846967 96-97 0.16491714179144243 98-99 0.1726434699206053 100-101 0.1604545212340811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 750680.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.78228964517231 #Duplication Level Percentage of deduplicated Percentage of total 1 85.31431049721759 62.093708574866554 2 9.441437799059168 13.74338921116005 3 2.4155207825938514 5.27421399728037 4 0.9854101929046384 2.868816403171621 5 0.500610282483209 1.8217781289522224 6 0.30065691434574743 1.3129499174241575 7 0.19667534742396228 1.0020137471593 8 0.13791479305877552 0.8030203531806239 9 0.1085683506631294 0.7111667829836268 >10 0.5634478225353629 7.256934699716491 >50 0.028284655025319665 1.3687017331912303 >100 0.006244285426305467 0.6720859422940164 >500 3.6731090517511176E-4 0.21159746258360895 >1k 5.509663577626675E-4 0.8596230460361272 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2843 0.3787232908829328 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2170 0.28907124207385304 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1418 0.18889540150263762 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 961 0.12801726434699207 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 6.660627697554217E-5 0.0 0.0 0.0 0.0 18-19 2.664251079021687E-4 0.0 0.0 6.660627697554217E-5 0.0 20-21 3.99637661853253E-4 0.0 0.0 1.998188309266265E-4 0.0 22-23 3.99637661853253E-4 0.0 0.0 4.6624393882879517E-4 0.0 24-25 5.328502158043374E-4 0.0 0.0 7.99275323706506E-4 0.0 26-27 5.328502158043374E-4 0.0 0.0 0.0013321255395108435 0.0 28-29 5.994564927798796E-4 0.0 0.0 0.002264613417168434 0.0 30-31 6.660627697554218E-4 0.0 0.0 0.0041295891724836145 0.0 32-33 6.660627697554218E-4 0.0 0.0 0.006793840251505302 0.0 34-35 6.660627697554218E-4 0.0 0.0 0.010190760377257952 0.0 36-37 6.660627697554218E-4 0.0 0.0 0.015785687643203497 0.0 38-39 6.660627697554218E-4 0.0 0.0 0.024511109926999522 0.0 40-41 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.03336974476474663 0.0 42-43 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.04122928544786061 0.0 44-45 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.046890818990781694 0.0 46-47 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.05641551659828423 0.0 48-49 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.06447487611232483 0.0 50-51 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.07233441679543881 0.0 52-53 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.0876538604998135 0.0 54-55 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.11636116587627218 0.0 56-57 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.16245270954334737 1.3321255395108434E-4 58-59 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.2130734800447594 1.3321255395108434E-4 60-61 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.2776149624340598 1.3321255395108434E-4 62-63 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.3211754675760644 1.3321255395108434E-4 64-65 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.37292854478606063 1.3321255395108434E-4 66-67 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.455653540789684 1.3321255395108434E-4 68-69 6.660627697554218E-4 0.0 1.3321255395108434E-4 0.5652208664144509 1.3321255395108434E-4 70-71 7.32669046730964E-4 0.0 1.3321255395108434E-4 0.694836681408856 1.3321255395108434E-4 72-73 7.99275323706506E-4 0.0 1.3321255395108434E-4 0.8390392710609047 1.3321255395108434E-4 74-75 7.99275323706506E-4 0.0 1.3321255395108434E-4 0.9822427665583204 1.3321255395108434E-4 76-77 7.99275323706506E-4 0.0 1.3321255395108434E-4 1.1265785687643204 1.3321255395108434E-4 78-79 7.99275323706506E-4 0.0 1.3321255395108434E-4 1.2546624393882881 1.3321255395108434E-4 80-81 0.0010657004316086747 0.0 1.3321255395108434E-4 1.348377471092876 1.3321255395108434E-4 82-83 0.0011989129855597591 0.0 1.3321255395108434E-4 1.4918473916981936 1.3321255395108434E-4 84-85 0.0012655192625353013 0.0 1.3321255395108434E-4 1.7517450844567592 1.3321255395108434E-4 86-87 0.0013321255395108435 0.0 1.3321255395108434E-4 2.084310225395641 1.3321255395108434E-4 88-89 0.0013321255395108435 0.0 1.3321255395108434E-4 2.4535088186710716 1.3321255395108434E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1800 0.0 45.690193 1 GTATCAA 3320 0.0 42.240917 1 ATCAACG 3835 0.0 36.43228 3 TCAACGC 3865 0.0 36.15191 4 CAACGCA 3920 0.0 35.523434 5 TATCAAC 3990 0.0 35.25991 2 AACGCAG 4015 0.0 34.66671 6 ACGCAGA 4505 0.0 30.581776 7 CGCAGAG 4510 0.0 30.33922 8 GCAGAGT 4975 0.0 27.312498 9 GTACATG 3110 0.0 24.610186 1 TACATGG 3050 0.0 24.465958 2 GAGTACT 2800 0.0 23.499102 12-13 TAGCCCT 470 0.0 23.257519 4 GTGTAGC 440 0.0 22.689035 1 GGTATAG 150 3.0122803E-5 22.184832 1 CAGAGTA 4795 0.0 22.144348 10-11 ACATGGG 3120 0.0 21.933722 3 GTACTTT 2960 0.0 21.58185 14-15 AGAGTAC 4550 0.0 21.457266 10-11 >>END_MODULE