FastQCFastQC Report
Thu 26 May 2016
SRR937714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750680
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40110.5343155538977993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.4302765492620025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.2829434645921032No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.11296424575051953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20650.051.542811
GTATCAA35650.037.319741
ATCAACG43850.030.2244913
TATCAAC44550.029.6429542
TCAACGC44600.029.5032084
CAACGCA45100.029.1761255
AACGCAG45900.028.6637886
GTACTAG1405.7845136E-727.1520141
ACGCAGA52350.025.133827
CGCAGAG53400.024.6396188
TGGTATC9850.024.595652
GTACATG32750.023.7942831
TACATGG33150.023.2143142
GCAGAGT56100.023.199749
ACATGGG31900.023.0815723
GTGGTAT10550.022.9698311
GAGTACT35550.022.31373812-13
CAGAGTA55500.020.79738410-11
GTACTTT37800.020.48152514-15
AGAGTAC53100.020.39556510-11
Produced by FastQC (version 0.11.5)