##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937714_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 750680 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42685032237438 33.0 31.0 34.0 31.0 34.0 2 32.63398785101508 34.0 31.0 34.0 31.0 34.0 3 32.71724036873235 34.0 31.0 34.0 31.0 34.0 4 36.28106383545585 37.0 35.0 37.0 35.0 37.0 5 36.213989982415946 37.0 35.0 37.0 35.0 37.0 6 36.2590930889327 37.0 37.0 37.0 35.0 37.0 7 36.21620797143923 37.0 37.0 37.0 35.0 37.0 8 36.199339265732405 37.0 36.0 37.0 35.0 37.0 9 37.917276336121915 39.0 38.0 39.0 35.0 39.0 10-11 37.85817192412213 39.0 38.0 39.0 35.0 39.0 12-13 37.77494671497842 39.0 37.5 39.0 35.0 39.0 14-15 39.051726434699205 40.0 38.0 41.0 36.0 41.0 16-17 39.0166908669473 40.0 38.0 41.0 36.0 41.0 18-19 39.000027308573564 40.0 38.0 41.0 36.0 41.0 20-21 38.89914011296425 40.0 38.0 41.0 35.0 41.0 22-23 38.744347791335855 40.0 38.0 41.0 35.0 41.0 24-25 38.83497628816539 40.0 38.0 41.0 35.0 41.0 26-27 38.73218615122289 40.0 38.0 41.0 35.0 41.0 28-29 38.59462620557362 40.0 38.0 41.0 34.5 41.0 30-31 38.634997602174025 40.0 38.0 41.0 35.0 41.0 32-33 38.56331992326957 40.0 38.0 41.0 35.0 41.0 34-35 38.44996736292428 40.0 38.0 41.0 35.0 41.0 36-37 38.294699472478285 40.0 38.0 41.0 34.0 41.0 38-39 38.15013787499334 40.0 38.0 41.0 34.0 41.0 40-41 37.98385996696329 40.0 38.0 41.0 33.5 41.0 42-43 37.772333084669896 40.0 37.5 41.0 33.0 41.0 44-45 37.45223730484361 40.0 37.0 41.0 33.0 41.0 46-47 37.09195595992966 40.0 37.0 41.0 32.0 41.0 48-49 36.766239276389406 40.0 37.0 41.0 31.0 41.0 50-51 36.52570669259871 40.0 36.5 41.0 31.0 41.0 52-53 36.28609394149305 39.0 36.0 41.0 30.0 41.0 54-55 36.07771953428891 39.0 36.0 40.5 30.0 41.0 56-57 35.836526882293384 39.0 35.0 40.0 29.0 41.0 58-59 35.58160201417381 39.0 35.0 40.0 28.5 41.0 60-61 35.24285847498268 38.0 35.0 40.0 28.0 41.0 62-63 34.9060738263974 38.0 34.0 40.0 27.5 41.0 64-65 34.5329721052912 37.5 34.0 40.0 26.0 41.0 66-67 34.11069097351734 37.0 34.0 39.5 26.0 41.0 68-69 33.68278161133905 36.0 33.0 39.0 26.0 40.0 70-71 33.266084083764056 36.0 33.0 39.0 25.0 40.0 72-73 32.765297463632976 35.0 32.0 38.0 23.5 40.0 74-75 32.25693504555869 35.0 32.0 37.5 22.0 39.0 76-77 31.032484547343742 34.0 30.5 36.0 21.5 38.5 78-79 31.604504582511858 35.0 31.0 36.0 22.0 39.0 80-81 31.390093648425427 35.0 31.0 36.0 20.5 38.0 82-83 31.028606063835454 34.0 31.0 36.0 20.0 37.0 84-85 30.622934539350986 34.0 31.0 35.0 18.0 37.0 86-87 30.179597831299624 34.0 30.5 35.0 15.0 36.0 88-89 29.805327836095273 34.0 30.0 35.0 8.5 36.0 90-91 29.27826503969734 34.0 29.5 35.0 4.5 35.5 92-93 28.808871956093142 34.0 29.0 35.0 2.0 35.0 94-95 28.338202030159323 34.0 29.0 35.0 2.0 35.0 96-97 27.800039297703414 33.0 28.0 35.0 2.0 35.0 98-99 26.928363616987266 33.0 26.5 35.0 2.0 35.0 100-101 24.219806042521448 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16072158294157646 1101 2 0.08466248120076614 1101 3 0.12428049396780949 1101 4 0.019275678731112578 1101 5 0.026915480216324283 1101 6 0.043155254273806065 1101 7 0.053624820791995376 1101 8 0.06513943786247722 1101 9 0.011211279385108242 1101 10-11 0.10063249200105417 1101 12-13 0.11863245421038471 1101 14-15 0.21949700466046096 1101 16-17 0.10568893597042717 1101 18-19 0.09058346165329567 1101 20-21 0.14517242226646232 1101 22-23 -0.0036049535412772116 1101 24-25 0.13569146414510413 1101 26-27 -0.055103605998162664 1101 28-29 -0.030035480193774333 1101 30-31 0.16626378613136694 1101 32-33 0.2939346473893494 1101 34-35 0.28193056786700055 1101 36-37 0.23279079825741888 1101 38-39 0.28049433489966447 1101 40-41 0.23574821006066315 1101 42-43 0.258541082917624 1101 44-45 0.20560883974116706 1101 46-47 0.45449202870089067 1101 48-49 0.6065757175593873 1101 50-51 0.4903126788510761 1101 52-53 0.5908184490483777 1101 54-55 0.6152981739029926 1101 56-57 0.5490137543225373 1101 58-59 0.6479961433530335 1101 60-61 0.7512274261600496 1101 62-63 0.7502368224694465 1101 64-65 0.7843142394540408 1101 66-67 0.7077280596814433 1101 68-69 0.6610743460087818 1101 70-71 0.7934044218281997 1101 72-73 0.7743067171330296 1101 74-75 0.7685059915396906 1101 76-77 0.5064864026047253 1101 78-79 0.6017681134629562 1101 80-81 0.7867326115873112 1101 82-83 0.6620204817054223 1101 84-85 0.30662901118871133 1101 86-87 0.3204979693241512 1101 88-89 0.5601887711027196 1101 90-91 0.6700912315219725 1101 92-93 0.7769950479024779 1101 94-95 0.733470198427689 1101 96-97 0.9589143408120897 1101 98-99 0.9388220677347263 1101 100-101 1.2857132968158318 1102 1 0.1273153185364393 1102 2 0.15893528362983744 1102 3 0.11174960981132642 1102 4 0.06430221011865456 1102 5 0.08446629708517861 1102 6 0.07151447483829543 1102 7 0.08471872088239962 1102 8 0.08238554676512422 1102 9 0.23177765310202147 1102 10-11 0.15034125475888516 1102 12-13 0.10604076797550022 1102 14-15 0.13216212472335087 1102 16-17 0.26804415396708947 1102 18-19 0.24048368025798084 1102 20-21 0.17619420826388676 1102 22-23 0.2738537835288497 1102 24-25 0.060066875973141975 1102 26-27 -0.0017297505148050618 1102 28-29 -0.06384049311304096 1102 30-31 -0.02819162991981017 1102 32-33 0.14299230944102703 1102 34-35 -0.0900127136918698 1102 36-37 0.002670485555626101 1102 38-39 0.19654598932893208 1102 40-41 0.1028857707566857 1102 42-43 0.15807059737768014 1102 44-45 0.1982871383010476 1102 46-47 0.23789711477118658 1102 48-49 0.3934941268261909 1102 50-51 0.46259305844458254 1102 52-53 0.34182639668128445 1102 54-55 0.3062435660952545 1102 56-57 0.39879397130583527 1102 58-59 0.22862205597338203 1102 60-61 0.21601783809648367 1102 62-63 0.22421871759009804 1102 64-65 0.24012903915125605 1102 66-67 0.19017416971529144 1102 68-69 0.24818864166147847 1102 70-71 0.0889529555554418 1102 72-73 0.1464776529854248 1102 74-75 0.12119009693373073 1102 76-77 0.0064246578584494785 1102 78-79 0.1478471335408429 1102 80-81 0.08573178109691071 1102 82-83 0.31871592173807883 1102 84-85 0.5582972976730254 1102 86-87 0.4975013035404494 1102 88-89 0.41525028489536453 1102 90-91 0.4106619603645001 1102 92-93 0.3717232720387358 1102 94-95 0.5418564547070339 1102 96-97 0.5853294031365799 1102 98-99 0.4166019717522822 1102 100-101 0.9977843475794437 1103 1 0.13475019731797744 1103 2 0.13853558736378346 1103 3 0.030565707710501044 1103 4 0.06552044736127982 1103 5 0.0910945201315414 1103 6 0.09485686883405009 1103 7 0.12249397977156917 1103 8 0.09901167888939 1103 9 0.1697344920680024 1103 10-11 0.13397483716872927 1103 12-13 0.10683091643097242 1103 14-15 0.2378697918045347 1103 16-17 0.06351895835582155 1103 18-19 0.14583754131070492 1103 20-21 0.11644665959492073 1103 22-23 0.15800679500690507 1103 24-25 0.11373809498071807 1103 26-27 0.05798698456536755 1103 28-29 -0.08788334601116787 1103 30-31 0.017305734417853103 1103 32-33 0.2052825294280467 1103 34-35 0.1192676789612861 1103 36-37 0.132917324411558 1103 38-39 0.019883981383422622 1103 40-41 -0.06938475054320747 1103 42-43 0.07954766876616048 1103 44-45 -0.08470596165603439 1103 46-47 0.036553972445418026 1103 48-49 -0.18917267923527703 1103 50-51 -0.17925399610647474 1103 52-53 -0.27162923725327914 1103 54-55 -0.2475712662458065 1103 56-57 -0.2613348955244348 1103 58-59 -0.16317492649589127 1103 60-61 -0.2629672943469217 1103 62-63 -0.3348822959568736 1103 64-65 -0.3566564432464858 1103 66-67 -0.12191961364316484 1103 68-69 -0.13579764629150048 1103 70-71 -0.07363659643699094 1103 72-73 -0.09691723415980391 1103 74-75 -0.091077012861696 1103 76-77 -0.23695262152175545 1103 78-79 -0.13690509219070535 1103 80-81 -0.06848307765579875 1103 82-83 0.0177271356834261 1103 84-85 -0.15301238989919597 1103 86-87 -0.16540463797975846 1103 88-89 -0.12950205088471378 1103 90-91 0.1320003706034143 1103 92-93 0.11447382503260783 1103 94-95 0.3698929688097543 1103 96-97 0.27026871137151076 1103 98-99 0.06879578922578844 1103 100-101 0.46281422575571796 1104 1 0.09974529572684787 1104 2 0.064883524925051 1104 3 0.007543311209481374 1104 4 0.015531758725430223 1104 5 0.007322557232818383 1104 6 -0.07506244343501578 1104 7 -0.01411713552894156 1104 8 0.017177729715122325 1104 9 0.06030081436647805 1104 10-11 -0.03946607980585526 1104 12-13 0.0790577469867344 1104 14-15 0.035256866685763555 1104 16-17 0.15422101702409918 1104 18-19 0.010877885176171276 1104 20-21 -0.10203610942702568 1104 22-23 0.053881917546689806 1104 24-25 -0.12646626366493052 1104 26-27 -0.03061116037090983 1104 28-29 -0.1690650065194248 1104 30-31 -0.010429730479209809 1104 32-33 -0.016801023848486807 1104 34-35 -0.2048481494076171 1104 36-37 -0.1699783848109675 1104 38-39 -0.22055791590938867 1104 40-41 -0.0476857836477933 1104 42-43 -0.20653181241926433 1104 44-45 -0.309289326409818 1104 46-47 -0.14440146077982519 1104 48-49 -0.1778801673583459 1104 50-51 -0.09012798750983819 1104 52-53 -0.06905778713639421 1104 54-55 -0.11991121044307818 1104 56-57 0.006129850723958441 1104 58-59 0.1945884397660862 1104 60-61 -0.20962290101308412 1104 62-63 -0.054756362769332156 1104 64-65 -0.21794141421398194 1104 66-67 -0.1635039587151823 1104 68-69 -0.02338153890895711 1104 70-71 -0.11353177779586332 1104 72-73 -0.061335452996999607 1104 74-75 -0.12123310968696899 1104 76-77 -0.2092133861699743 1104 78-79 -0.0175664299480367 1104 80-81 -0.23781796944374634 1104 82-83 0.007799528413990231 1104 84-85 -0.06396933130632831 1104 86-87 -0.01605692664577063 1104 88-89 0.16371113062497855 1104 90-91 -0.020506981783281475 1104 92-93 -0.2972070436801637 1104 94-95 -0.14717587969337487 1104 96-97 0.004515751564557746 1104 98-99 -6.182821112155068E-4 1104 100-101 0.15672117093330584 1105 1 0.19170180416200822 1105 2 0.051919693097872255 1105 3 0.10557680705819905 1105 4 0.10627042174739643 1105 5 0.07337996120254786 1105 6 0.09767781074177151 1105 7 0.05130788640043704 1105 8 0.027969023242384594 1105 9 0.1295446078427389 1105 10-11 0.06050740094728724 1105 12-13 0.11426918698184352 1105 14-15 0.01687494873223727 1105 16-17 0.031629678618109835 1105 18-19 0.1334182250305247 1105 20-21 0.06107969808404334 1105 22-23 0.23974409906073646 1105 24-25 0.010814697555765918 1105 26-27 0.13988821744676017 1105 28-29 0.08587267325046355 1105 30-31 -0.0025433144534261487 1105 32-33 0.08579378245902092 1105 34-35 0.1483769989981667 1105 36-37 0.11855306089596951 1105 38-39 0.05194060513262144 1105 40-41 0.1957598824952811 1105 42-43 0.3188991253420994 1105 44-45 0.04948344444348862 1105 46-47 -0.10888504944590949 1105 48-49 -0.29911086440655055 1105 50-51 -0.15123241214417504 1105 52-53 5.06277244177511E-4 1105 54-55 -0.00430259372779318 1105 56-57 0.02151917366718692 1105 58-59 -0.019931867857181373 1105 60-61 0.13230096834902128 1105 62-63 -0.2325872923709298 1105 64-65 -0.13105110189885494 1105 66-67 -0.19922742416876105 1105 68-69 -0.11923229674743396 1105 70-71 -0.0428692046129342 1105 72-73 0.016021190074333447 1105 74-75 -0.12017437975489287 1105 76-77 -0.19323655991184197 1105 78-79 0.17229199807260542 1105 80-81 0.06746344056420739 1105 82-83 0.010988977833623181 1105 84-85 0.14415983782527064 1105 86-87 0.008185744862355904 1105 88-89 0.1685376335522939 1105 90-91 0.16909951339225415 1105 92-93 0.32097292000887023 1105 94-95 0.37316642258523913 1105 96-97 0.6502420729370293 1105 98-99 0.6521672587794249 1105 100-101 0.9745727039497716 1106 1 0.06898601700120821 1106 2 0.15707588015613538 1106 3 0.1595007093543117 1106 4 0.07867676444340788 1106 5 0.1399196762939141 1106 6 0.03467198805893901 1106 7 0.13242261888661488 1106 8 0.0345973602988181 1106 9 -0.0022897203514986586 1106 10-11 -0.07963981405253406 1106 12-13 -0.09987004471699379 1106 14-15 0.20693610521225736 1106 16-17 0.20066142871881 1106 18-19 0.09603769989044508 1106 20-21 0.12091783482554064 1106 22-23 0.060623360788497394 1106 24-25 0.16261643337442422 1106 26-27 0.029193157572002804 1106 28-29 0.08931501404220654 1106 30-31 0.22135531959645505 1106 32-33 0.3379818661005771 1106 34-35 0.06999965985436063 1106 36-37 0.07660411007750412 1106 38-39 0.12561219117282718 1106 40-41 0.19655638156146438 1106 42-43 0.06531030196347842 1106 44-45 0.25688447714572504 1106 46-47 0.267584354003759 1106 48-49 0.5912011435035041 1106 50-51 0.3339545362573304 1106 52-53 0.4899229103419174 1106 54-55 0.5637111023940804 1106 56-57 0.40219079632804267 1106 58-59 0.401498534603725 1106 60-61 0.4567507431320479 1106 62-63 0.6302775408220214 1106 64-65 0.21921622882221214 1106 66-67 0.021888040730345892 1106 68-69 0.11730046042105613 1106 70-71 0.3347903698551491 1106 72-73 0.056944762784631564 1106 74-75 0.24244326407269057 1106 76-77 -0.01903214346184612 1106 78-79 0.09281603952717887 1106 80-81 0.012147953692711155 1106 82-83 -0.05354575684978968 1106 84-85 0.15379106791279185 1106 86-87 0.2665627177996974 1106 88-89 -0.16029746367552278 1106 90-91 0.22681365262395303 1106 92-93 0.3087050033574634 1106 94-95 0.42432546605394705 1106 96-97 0.3835613683557888 1106 98-99 0.7078489521181304 1106 100-101 1.1201214643408797 1107 1 0.3666132069838426 1107 2 0.28974504715476 1107 3 0.12326969671986632 1107 4 0.018157707554593117 1107 5 0.13028788650218104 1107 6 0.19147136272541587 1107 7 0.17200577997763844 1107 8 0.14100176769759543 1107 9 0.19864377031735359 1107 10-11 0.2135980156192474 1107 12-13 0.2779118251204338 1107 14-15 0.16774565658055707 1107 16-17 0.2646867126367738 1107 18-19 0.23868627297254363 1107 20-21 0.35279669148736303 1107 22-23 0.28282157347705805 1107 24-25 0.23909013855071493 1107 26-27 0.2604500629226649 1107 28-29 0.38733867449812465 1107 30-31 0.3780363168230352 1107 32-33 0.10377867780152883 1107 34-35 0.06387785510573707 1107 36-37 0.16454727387429813 1107 38-39 0.4453120761775935 1107 40-41 0.25708107547121983 1107 42-43 0.25148231377867347 1107 44-45 0.1831390901078649 1107 46-47 0.17521988677739841 1107 48-49 0.0736775243857366 1107 50-51 0.07621678550385269 1107 52-53 -0.03337301822575256 1107 54-55 -0.0037584013225924195 1107 56-57 -0.28656672909492187 1107 58-59 -0.4257699905261134 1107 60-61 -0.3282683099987551 1107 62-63 -0.13470566085555902 1107 64-65 -0.046860554919121 1107 66-67 0.24478712302333605 1107 68-69 -0.003919917033861964 1107 70-71 -0.005820983856644091 1107 72-73 0.1701305063828542 1107 74-75 0.014450193850443327 1107 76-77 -0.08668766886977863 1107 78-79 0.1950020762504927 1107 80-81 0.2627886950737057 1107 82-83 0.24988944371541422 1107 84-85 -0.14795866338921115 1107 86-87 -0.006278491078234083 1107 88-89 0.23726847067948498 1107 90-91 0.4627643174166245 1107 92-93 0.3634066546288004 1107 94-95 0.6944158087096604 1107 96-97 0.8525389630765297 1107 98-99 1.0986373082876604 1107 100-101 1.1835492240384333 1108 1 0.2041779475139549 1108 2 0.2141213377339568 1108 3 0.054052337794985306 1108 4 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-0.38185043156181564 2316 68-69 -0.010470135374468725 2316 70-71 0.035663732300562856 2316 72-73 0.16467199109901287 2316 74-75 -0.1656808105163705 2316 76-77 -0.30830338939379587 2316 78-79 -0.08665731239579699 2316 80-81 -0.22956938352891498 2316 82-83 -0.28504505410117886 2316 84-85 -0.3324564799830938 2316 86-87 -0.604531766187403 2316 88-89 -0.4308538000628701 2316 90-91 -0.003392887823551405 2316 92-93 0.06864682930129007 2316 94-95 -0.4158462000239638 2316 96-97 -0.12235186661779451 2316 98-99 -0.14153736420142238 2316 100-101 0.22066712796856436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 7.0 6 23.0 7 42.0 8 124.0 9 251.0 10 430.0 11 749.0 12 1313.0 13 1978.0 14 2648.0 15 3856.0 16 5373.0 17 7953.0 18 7357.0 19 3855.0 20 3111.0 21 3127.0 22 3102.0 23 3510.0 24 3934.0 25 4767.0 26 5744.0 27 7148.0 28 8784.0 29 11243.0 30 14256.0 31 18071.0 32 24124.0 33 32764.0 34 46907.0 35 70379.0 36 112888.0 37 179502.0 38 148041.0 39 13316.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58497328554488 20.126577218765405 15.729018160499914 21.5594313351898 2 15.181568711035329 20.728006607342675 41.35530452389833 22.735120157723664 3 19.36617466830074 25.04635796877498 30.460515799008895 25.126951563915384 4 12.756167741247934 17.705413758192574 38.123301539937124 31.41511696062237 5 13.390483829491842 37.73771439763475 34.62099601216735 14.25080576070606 6 30.798209623274897 37.53756594021421 17.613896733628177 14.05032770288272 7 27.745111099269998 33.57249427186018 20.78755261895881 17.894842009911013 8 25.660867480151328 36.35277348537326 19.56879096286034 18.41756807161507 9 26.043320722544895 17.523045771833537 20.8352427132733 35.59839079234827 10-11 24.55833377737518 27.779746363297274 27.222851281504767 20.439068577822773 12-13 25.467106547118295 24.969743232108875 28.303749308107484 21.259400912665345 14-15 22.36272446315341 26.348976927585653 26.869571588426496 24.418727020834442 16-17 21.236612138327914 29.713526402728192 27.545025843235464 21.504835615708423 18-19 21.06816486385677 27.885117493472585 30.608048702509727 20.43866894016092 20-21 21.91972611498908 26.910268023658553 30.88912719134651 20.280878670005862 22-23 21.635333650826425 26.860648285370786 30.677359301159964 20.82665876264283 24-25 21.291961954494592 27.026629189534823 31.002224649650984 20.679184206319604 26-27 21.263515327195307 27.195239487634804 30.75236903295765 20.78887615221224 28-29 21.071148345946494 27.36271948696566 30.893160918698072 20.672971248389775 30-31 21.33925241114723 27.165703095859755 30.793813608994512 20.70123088399851 32-33 21.30601587893643 27.076117653327646 30.710089518836252 20.907776948899663 34-35 21.481123781105133 27.192745244311823 30.651942239036607 20.674188735546437 36-37 21.591357169499652 27.052539031278304 30.568750999094153 20.787352800127884 38-39 21.24648401881229 27.379061237185777 30.572501313809685 20.801953430192246 40-41 21.524542709122905 27.26547315668249 30.51802986927695 20.69195426491765 42-43 21.542697412160965 27.249053692696947 30.177376826829427 21.03087206831266 44-45 21.58284594157693 27.355282780467565 30.089738696740753 20.97213258121475 46-47 21.586961155219267 27.786873234933662 29.455293866894017 21.170871742953057 48-49 21.731696595087122 27.897572867267012 29.284315553897798 21.08641498374807 50-51 21.80809399477807 28.014733308466987 28.988383865295464 21.188788831459476 52-53 21.882826237544624 28.20988970000533 28.71143496563116 21.195849096818883 54-55 21.740155592263015 28.203828528800557 28.814341663558373 21.241674215378055 56-57 21.76220226994192 28.32252091437097 28.832192145787822 21.08308466989929 58-59 21.838266638247987 28.029852933340436 28.89346992060532 21.238410507806254 60-61 21.83120637288858 28.131027868066287 29.018090264826558 21.019675494218575 62-63 21.816486385676985 28.05343155538978 29.090424681621997 21.039657377311237 64-65 21.763867426866305 27.92448180316513 29.01875632759631 21.292894442372248 66-67 21.678411573506686 28.164797250492885 28.84118399317952 21.31560718282091 68-69 21.735574236408482 28.040395401766 28.926992145116525 21.297038216708994 70-71 21.83153940427346 28.13575691373155 28.74447167901103 21.28823200298396 72-73 21.87783076677146 28.07208131294293 28.64582778281025 21.404260137475354 74-75 21.859713859434112 28.30367133798689 28.423296211434966 21.41331859114403 76-77 21.78937763094794 28.144082698353497 28.671138168060956 21.395401502637608 78-79 21.820815793680396 28.152541695529386 28.45759844407737 21.56904406671285 80-81 21.737572426048665 28.528752949827457 28.363436060262735 21.37023856386114 82-83 21.833380513879405 28.53526051987411 28.21301183954174 21.418347126704745 84-85 21.838330943263767 28.364519689266533 28.193540351323577 21.603609016146123 86-87 21.648105717482817 28.34436777321895 28.422230511003356 21.585295998294878 88-89 21.728299674961367 28.226741088080143 28.323786433633508 21.721172803324983 90-91 21.617997378013683 28.27615058301891 28.435361642258744 21.67049039670866 92-93 21.77380410796582 27.96639239819173 28.60342883329215 21.656374660550302 94-95 21.535008259178344 28.165529919539615 28.43848244258539 21.860979378696648 96-97 21.650769968561836 28.115175574146107 28.432354665103638 21.801699792188415 98-99 21.609516958409934 28.20477979378147 28.167878933205447 22.01782431460315 100-101 21.419111131837894 28.462701780800927 28.327193023364266 21.79099406399691 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 128.5 2 102.5 3 126.5 4 112.0 5 115.5 6 199.0 7 436.5 8 780.0 9 959.0 10 904.0 11 842.0 12 938.5 13 1058.5 14 1126.0 15 1223.5 16 1293.5 17 1317.0 18 1394.5 19 1485.0 20 1609.0 21 1834.0 22 2094.5 23 2494.5 24 3120.0 25 3962.0 26 4900.5 27 6040.0 28 7248.5 29 8656.5 30 10295.0 31 11918.0 32 13614.5 33 15419.5 34 17549.5 35 19546.0 36 21773.5 37 23693.5 38 24735.5 39 25407.0 40 26184.5 41 27531.5 42 28013.5 43 28140.5 44 29225.0 45 29991.0 46 29597.0 47 29186.5 48 29295.0 49 28349.0 50 26679.5 51 24957.0 52 23194.5 53 21918.5 54 20688.0 55 18688.5 56 16217.0 57 14171.5 58 12551.5 59 10859.5 60 8740.0 61 6826.5 62 5268.5 63 3838.0 64 2752.5 65 1983.0 66 1415.0 67 1065.5 68 790.5 69 557.5 70 392.0 71 272.0 72 208.5 73 161.5 74 128.5 75 103.0 76 75.0 77 45.0 78 28.5 79 19.0 80 10.0 81 6.5 82 8.5 83 6.0 84 2.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01998188309266265 2 0.0 3 0.0 4 0.0 5 0.0195822454308094 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002864069909948313 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.990941546331325E-4 24-25 0.0 26-27 5.994564927798796E-4 28-29 0.0011989129855597591 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.660627697554218E-5 40-41 0.0011989129855597591 42-43 0.01745084456759205 44-45 6.660627697554218E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.660627697554218E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.660627697554218E-5 82-83 0.002331219694143976 84-85 5.994564927798796E-4 86-87 0.0 88-89 0.0 90-91 0.013854105610912774 92-93 0.0021980071401928917 94-95 0.0 96-97 0.0 98-99 0.002664251079021687 100-101 0.02331219694143976 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 750680.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.60833012719124 #Duplication Level Percentage of deduplicated Percentage of total 1 83.57900703154522 58.177951131546365 2 10.414292879760405 14.498430736312388 3 2.744486753556118 5.731174199137128 4 1.1397033442067959 3.1733138652244195 5 0.5736054733709788 1.9963859576585445 6 0.35264813127986144 1.4728348524519403 7 0.22904354283952577 1.1160336980432624 8 0.1625248485218277 0.9050466647823304 9 0.1236935760695813 0.774909294989783 >10 0.6338307239015339 7.82983014826589 >50 0.0348437392847507 1.6581139308219774 >100 0.011549958437624476 1.297478940786785 >500 1.9249930644800323E-4 0.11362822871069791 >1k 5.774979193440097E-4 1.2548683512684975 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4011 0.5343155538977993 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3230 0.4302765492620025 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2124 0.2829434645921032 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 848 0.11296424575051953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.3321255395108434E-4 0.0 0.0 0.0 0.0 8 1.3321255395108434E-4 0.0 0.0 0.0 0.0 9 1.3321255395108434E-4 0.0 0.0 0.0 0.0 10-11 1.3321255395108434E-4 0.0 0.0 0.0 0.0 12-13 1.3321255395108434E-4 0.0 0.0 0.0 0.0 14-15 1.3321255395108434E-4 0.0 0.0 0.0 0.0 16-17 1.3321255395108434E-4 0.0 0.0 0.0 0.0 18-19 1.3321255395108434E-4 0.0 0.0 6.660627697554217E-5 0.0 20-21 1.3321255395108434E-4 0.0 0.0 1.998188309266265E-4 0.0 22-23 1.3321255395108434E-4 0.0 0.0 4.6624393882879517E-4 0.0 24-25 1.3321255395108434E-4 0.0 0.0 7.99275323706506E-4 0.0 26-27 1.3321255395108434E-4 0.0 0.0 0.0013321255395108435 0.0 28-29 1.3321255395108434E-4 0.0 0.0 0.002264613417168434 0.0 30-31 1.3321255395108434E-4 0.0 0.0 0.004196195449459157 0.0 32-33 1.3321255395108434E-4 0.0 0.0 0.00706026535940747 0.0 34-35 1.3321255395108434E-4 0.0 0.0 0.010323972931209038 0.0 36-37 2.664251079021687E-4 0.0 0.0 0.015852293920179037 0.0 38-39 3.99637661853253E-4 0.0 0.0 0.02444450365002398 0.0 40-41 3.99637661853253E-4 0.0 0.0 0.03356956359567326 0.0 42-43 5.994564927798796E-4 0.0 0.0 0.04202856077156711 0.0 44-45 6.660627697554218E-4 0.0 0.0 0.04742366920658603 0.0 46-47 6.660627697554218E-4 0.0 0.0 0.056815154260137474 0.0 48-49 6.660627697554218E-4 0.0 0.0 0.06454148238930037 0.0 50-51 6.660627697554218E-4 0.0 0.0 0.07253423562636543 0.0 52-53 6.660627697554218E-4 0.0 0.0 0.08785367933074012 0.0 54-55 6.660627697554218E-4 0.0 0.0 0.11649437843022326 0.0 56-57 6.660627697554218E-4 0.0 0.0 0.16258592209729844 0.0 58-59 6.660627697554218E-4 0.0 0.0 0.21433899930729472 0.0 60-61 6.660627697554218E-4 0.0 0.0 0.2786140565886929 0.0 62-63 6.660627697554218E-4 0.0 0.0 0.3226408056695263 0.0 64-65 6.660627697554218E-4 0.0 0.0 0.3749267330953269 0.0 66-67 6.660627697554218E-4 0.0 0.0 0.45685245377524375 0.0 68-69 6.660627697554218E-4 0.0 0.0 0.5672190547237171 0.0 70-71 6.660627697554218E-4 0.0 0.0 0.6973677199339265 0.0 72-73 6.660627697554218E-4 0.0 0.0 0.8439015292801193 0.0 74-75 6.660627697554218E-4 0.0 0.0 0.9891698193637768 0.0 76-77 6.660627697554218E-4 0.0 0.0 1.13510417221719 0.0 78-79 6.660627697554218E-4 0.0 0.0 1.2645867746576438 0.0 80-81 0.0010657004316086747 0.0 0.0 1.358701444024085 0.0 82-83 0.0010657004316086747 0.0 0.0 1.5039697341077423 0.0 84-85 0.0010657004316086747 0.0 0.0 1.766198646560452 0.0 86-87 0.0010657004316086747 0.0 0.0 2.103093195502744 0.0 88-89 0.0010657004316086747 0.0 0.0 2.477020834443438 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2065 0.0 51.54281 1 GTATCAA 3565 0.0 37.31974 1 ATCAACG 4385 0.0 30.224491 3 TATCAAC 4455 0.0 29.642954 2 TCAACGC 4460 0.0 29.503208 4 CAACGCA 4510 0.0 29.176125 5 AACGCAG 4590 0.0 28.663788 6 GTACTAG 140 5.7845136E-7 27.152014 1 ACGCAGA 5235 0.0 25.13382 7 CGCAGAG 5340 0.0 24.639618 8 TGGTATC 985 0.0 24.59565 2 GTACATG 3275 0.0 23.794283 1 TACATGG 3315 0.0 23.214314 2 GCAGAGT 5610 0.0 23.19974 9 ACATGGG 3190 0.0 23.081572 3 GTGGTAT 1055 0.0 22.969831 1 GAGTACT 3555 0.0 22.313738 12-13 CAGAGTA 5550 0.0 20.797384 10-11 GTACTTT 3780 0.0 20.481525 14-15 AGAGTAC 5310 0.0 20.395565 10-11 >>END_MODULE