FastQCFastQC Report
Thu 26 May 2016
SRR937713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686993
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28000.40757329405103104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.3174704836876067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.2043688945884456No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8680.12634772115581963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.043.8862081
GTATCAA28400.036.996791
ATCAACG35400.029.2610473
CAACGCA36000.028.6476465
TCAACGC36100.028.5641214
TATCAAC36300.028.4129582
AACGCAG37150.027.8765686
GTGTACG700.00835196627.1676521
ACCGTAT350.008396557527.1399262-63
GTACATG31050.027.102031
TACATGG30700.026.3192772
ACATGGG30850.025.2595313
CGCAGAG42300.024.5967128
ACGCAGA42400.024.5386987
CATGGGG12500.023.569484
GCAGAGT45450.022.57849
GTGGTAT9500.022.5205541
GAGTACT26300.022.1269712-13
TGGTATC9800.021.8247762
ACCGGTG1100.00287313521.594877
Produced by FastQC (version 0.11.5)