##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937713_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 686993 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86749064400947 33.0 31.0 34.0 30.0 34.0 2 32.01685752256573 33.0 31.0 34.0 30.0 34.0 3 32.04167582493562 34.0 31.0 34.0 30.0 34.0 4 35.62246922457725 37.0 35.0 37.0 35.0 37.0 5 35.61247785639737 37.0 35.0 37.0 33.0 37.0 6 35.6595787730006 37.0 35.0 37.0 35.0 37.0 7 35.60761172239018 37.0 35.0 37.0 33.0 37.0 8 35.59399731874997 37.0 35.0 37.0 33.0 37.0 9 37.249903565247386 39.0 37.0 39.0 34.0 39.0 10-11 37.13698975098727 39.0 37.0 39.0 34.0 39.0 12-13 37.02318800919369 39.0 37.0 39.0 33.0 39.0 14-15 38.21797529232467 40.0 38.0 41.0 33.5 41.0 16-17 38.1482904483743 40.0 38.0 41.0 33.0 41.0 18-19 38.083519046045595 40.0 38.0 41.0 33.5 41.0 20-21 38.00203786647026 40.0 38.0 41.0 33.0 41.0 22-23 37.80577458576725 40.0 38.0 41.0 33.0 41.0 24-25 37.640881348136006 40.0 37.0 41.0 32.0 41.0 26-27 37.59185683114676 40.0 37.0 41.0 32.5 41.0 28-29 37.64978755242048 40.0 37.0 41.0 33.0 41.0 30-31 37.47620499772196 40.0 37.0 41.0 32.0 41.0 32-33 37.25339923405333 40.0 37.0 41.0 32.0 41.0 34-35 37.290455652386555 40.0 37.0 41.0 32.0 41.0 36-37 37.20173495217564 40.0 37.0 41.0 31.5 41.0 38-39 37.074505853771434 40.0 37.0 41.0 31.0 41.0 40-41 36.83838845519533 39.5 36.0 41.0 30.5 41.0 42-43 36.59791366142014 39.0 36.0 41.0 30.0 41.0 44-45 36.38324189620564 39.0 36.0 41.0 30.0 41.0 46-47 36.057329550665 39.0 36.0 41.0 29.5 41.0 48-49 35.715857366814504 39.0 35.0 40.5 27.5 41.0 50-51 34.830376728729405 38.0 34.0 39.5 26.0 40.5 52-53 34.94480220322478 38.5 34.5 39.5 26.5 40.5 54-55 35.309036627738564 39.0 35.0 40.0 27.0 41.0 56-57 35.12187242082525 39.0 35.0 40.0 26.0 41.0 58-59 34.88812840887753 38.0 34.5 40.0 26.0 41.0 60-61 34.57184498240885 38.0 34.0 40.0 25.0 41.0 62-63 34.21607643746006 38.0 34.0 40.0 23.5 41.0 64-65 33.85725545966262 37.5 34.0 40.0 22.5 41.0 66-67 33.455444960865684 37.0 33.0 39.5 22.0 41.0 68-69 32.97023332115465 36.0 32.5 39.0 20.5 41.0 70-71 32.53618304698884 36.0 32.0 39.0 18.5 40.0 72-73 32.07875553317137 35.0 31.5 38.5 17.5 40.0 74-75 31.596002433794812 35.0 31.0 37.5 13.0 39.5 76-77 31.08603799456472 35.0 31.0 37.0 8.0 39.0 78-79 30.523069376252742 34.5 30.0 36.5 7.0 39.0 80-81 29.98097724430962 34.0 29.5 36.0 2.0 38.0 82-83 29.428462880990054 34.0 29.0 35.5 2.0 37.0 84-85 28.92979331667135 34.0 29.0 35.0 2.0 37.0 86-87 28.432353750329334 34.0 28.5 35.0 2.0 36.0 88-89 27.92382600696077 33.5 27.0 35.0 2.0 36.0 90-91 27.41810688027389 33.0 26.0 35.0 2.0 35.5 92-93 26.85176559295364 33.0 25.5 35.0 2.0 35.0 94-95 26.192736316090556 32.0 24.5 35.0 2.0 35.0 96-97 25.435943306554798 32.0 21.5 34.0 2.0 35.0 98-99 24.467938537947255 32.0 10.0 34.0 2.0 35.0 100-101 22.034166286992736 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06268116838578308 1101 2 -0.015570292524355267 1101 3 -0.09581234573650477 1101 4 -0.023380420464178542 1101 5 -0.1118017624397396 1101 6 0.018871558144823553 1101 7 -0.015884160253861523 1101 8 0.0025563916259656594 1101 9 0.01833550841254805 1101 10-11 -0.05626051156673384 1101 12-13 0.0548230088962427 1101 14-15 0.3167639582767592 1101 16-17 0.3552749648614011 1101 18-19 0.25994599256489437 1101 20-21 0.2905633380250947 1101 22-23 0.31263338735743673 1101 24-25 0.18147972573014215 1101 26-27 0.38856848684471146 1101 28-29 0.1301690176916992 1101 30-31 0.10610955349951467 1101 32-33 0.3343315601471204 1101 34-35 0.28814516367949494 1101 36-37 0.31731500172900695 1101 38-39 0.20973705795000086 1101 40-41 0.38135973516001087 1101 42-43 0.5932333915202648 1101 44-45 0.6388438940189971 1101 46-47 0.654319015756343 1101 48-49 0.6611108028429129 1101 50-51 0.7630381155714119 1101 52-53 0.891015477707441 1101 54-55 0.7895824227871842 1101 56-57 0.7789451207767328 1101 58-59 0.8584606655360929 1101 60-61 0.9163610539086662 1101 62-63 0.9835681940804051 1101 64-65 0.7300143962138037 1101 66-67 0.9141456075609611 1101 68-69 0.999912686819485 1101 70-71 0.8751779071010333 1101 72-73 1.0152109807520056 1101 74-75 0.9741543361967757 1101 76-77 1.0831819040738075 1101 78-79 1.2279231323263389 1101 80-81 1.2227588770807003 1101 82-83 1.467598165676229 1101 84-85 1.4112491889034615 1101 86-87 1.5042175665844653 1101 88-89 1.4965374612737605 1101 90-91 1.8232418508190626 1101 92-93 1.4996654892358592 1101 94-95 1.3850001755436807 1101 96-97 1.5523936792363457 1101 98-99 1.8549968175682139 1101 100-101 2.4335398424506387 1102 1 0.04471179598603214 1102 2 -0.030471003627599913 1102 3 -0.04714160719235139 1102 4 -0.15011023073829222 1102 5 0.0370725699788963 1102 6 -0.06395279050230585 1102 7 -0.013479392026304993 1102 8 0.03269713695657828 1102 9 0.1457439649349297 1102 10-11 0.0962799859104706 1102 12-13 0.12293106118150376 1102 14-15 0.32496672597319076 1102 16-17 0.16400737169507806 1102 18-19 0.22216310035162223 1102 20-21 0.25462459322559283 1102 22-23 0.3381490109743268 1102 24-25 0.44361421571272786 1102 26-27 0.331938408062733 1102 28-29 0.10342888019206953 1102 30-31 0.27531376700753185 1102 32-33 0.30104101103378156 1102 34-35 0.3256920050238037 1102 36-37 0.434307713658427 1102 38-39 0.4411111690709504 1102 40-41 0.558751963185145 1102 42-43 0.6995595548084523 1102 44-45 0.9121170343807492 1102 46-47 1.0127770134548442 1102 48-49 1.027572400317169 1102 50-51 0.8829298272352801 1102 52-53 0.8978461997280363 1102 54-55 0.8720969546688053 1102 56-57 0.9546923693087948 1102 58-59 0.7185120066001005 1102 60-61 0.789793528606964 1102 62-63 1.0727659161895247 1102 64-65 1.0408933906371018 1102 66-67 0.947841869105325 1102 68-69 1.0956386907733418 1102 70-71 0.8367975111257095 1102 72-73 0.9576735814424531 1102 74-75 1.132485456553212 1102 76-77 0.9243860585691017 1102 78-79 1.1108050183727798 1102 80-81 1.2729270633248326 1102 82-83 0.9115729672459665 1102 84-85 1.0607652846220859 1102 86-87 1.3580799489278625 1102 88-89 1.4660278864603882 1102 90-91 1.6585226038213357 1102 92-93 1.8409876691656315 1102 94-95 2.0791003791375537 1102 96-97 1.914075246614118 1102 98-99 1.5367854930277751 1102 100-101 1.9717727066328727 1103 1 0.15926520123711185 1103 2 0.1126236278391275 1103 3 0.10481625406783834 1103 4 0.11759364161674313 1103 5 0.02758593258528208 1103 6 0.12986372889560727 1103 7 0.03755871715133452 1103 8 0.10671097841228061 1103 9 0.01446697319282464 1103 10-11 0.19539275944185164 1103 12-13 0.24057439975508288 1103 14-15 0.07259054917841468 1103 16-17 0.12110518344404397 1103 18-19 0.255578651209035 1103 20-21 0.23114742439958746 1103 22-23 0.23473562849117258 1103 24-25 0.2669368878726104 1103 26-27 0.10163899171680413 1103 28-29 0.19293756272401197 1103 30-31 0.039611484793617535 1103 32-33 0.08145246004988138 1103 34-35 0.15318428019050145 1103 36-37 0.2809133044500669 1103 38-39 0.32916433037992476 1103 40-41 0.6202298909421415 1103 42-43 0.3759025916166152 1103 44-45 0.4350611445341599 1103 46-47 0.413117628911742 1103 48-49 0.6758846823503859 1103 50-51 0.7120483830980149 1103 52-53 0.35880642813012287 1103 54-55 0.38058071896652024 1103 56-57 0.12404239912394388 1103 58-59 0.1069084938337923 1103 60-61 0.2809935430627917 1103 62-63 0.39130913008157364 1103 64-65 0.3500222462775895 1103 66-67 0.26081958905978553 1103 68-69 0.31370486232788153 1103 70-71 0.5097931201678279 1103 72-73 0.4745281738784435 1103 74-75 0.5084589662476589 1103 76-77 0.5248721357993631 1103 78-79 0.6306953347747282 1103 80-81 0.41100281186569987 1103 82-83 0.592672875090063 1103 84-85 0.5594823298507627 1103 86-87 0.5777227092682935 1103 88-89 0.6421405335032659 1103 90-91 0.7807511504684861 1103 92-93 0.7244917619720539 1103 94-95 0.5913200715898057 1103 96-97 0.9996763380291611 1103 98-99 1.4315289924224395 1103 100-101 2.0117475896310104 1104 1 0.11916675279393019 1104 2 0.11761620328314137 1104 3 0.09388403383329447 1104 4 -0.1178875514567892 1104 5 0.107893905506117 1104 6 0.09263571902737766 1104 7 0.19459684546169598 1104 8 0.2706650513950848 1104 9 0.3145359572563322 1104 10-11 0.36773891913411205 1104 12-13 0.24664558195485142 1104 14-15 0.5684193517787648 1104 16-17 0.6367552120899731 1104 18-19 0.6290601248957088 1104 20-21 0.555872365061596 1104 22-23 0.6312039952057802 1104 24-25 0.621935894034408 1104 26-27 0.5680209529688085 1104 28-29 0.5636304433348087 1104 30-31 0.7340413139703728 1104 32-33 0.5895025494953217 1104 34-35 0.6948816389193198 1104 36-37 0.8339374956411518 1104 38-39 0.8587719396728914 1104 40-41 0.9206057956272105 1104 42-43 1.0662810685887933 1104 44-45 1.0480693173918567 1104 46-47 1.3080601616791725 1104 48-49 1.2680427143168913 1104 50-51 1.2474231770227462 1104 52-53 1.1449324468142805 1104 54-55 1.2584263115863905 1104 56-57 1.1239985533208525 1104 58-59 1.0547933722932257 1104 60-61 1.123949568645358 1104 62-63 1.2655535207418325 1104 64-65 0.9632847247931409 1104 66-67 1.0901015701885584 1104 68-69 1.113086928809814 1104 70-71 1.1687253871712286 1104 72-73 1.1966013203701955 1104 74-75 1.368465163121158 1104 76-77 1.3740981112223274 1104 78-79 1.2195737006708782 1104 80-81 1.394242022875087 1104 82-83 1.5088923145653155 1104 84-85 1.6778981517549525 1104 86-87 1.728675040111188 1104 88-89 1.560227804189715 1104 90-91 1.6662697586273119 1104 92-93 2.0241427493795356 1104 94-95 2.1466651672905783 1104 96-97 2.3347868879139924 1104 98-99 2.870255944145285 1104 100-101 3.2659246331377396 1105 1 0.26624618145484646 1105 2 0.20331706622604884 1105 3 0.20093181301221463 1105 4 0.24670788739130245 1105 5 0.14998606813829696 1105 6 0.2243272354826331 1105 7 0.2871880643788529 1105 8 0.15646317055868053 1105 9 0.1337959857738369 1105 10-11 0.33453581023186985 1105 12-13 0.09344642923785784 1105 14-15 0.2731560594084286 1105 16-17 0.36406278076687215 1105 18-19 0.36771700000388563 1105 20-21 0.4472379907570243 1105 22-23 0.5375165832927138 1105 24-25 0.3776455759736024 1105 26-27 0.5595646070004108 1105 28-29 0.5033645547179049 1105 30-31 0.5207823239515008 1105 32-33 0.38479468904975533 1105 34-35 0.6337091245163364 1105 36-37 0.04939299267813624 1105 38-39 0.25479020406145736 1105 40-41 0.6427126291130278 1105 42-43 0.8922748540811298 1105 44-45 0.8755343092734265 1105 46-47 0.7940321881526486 1105 48-49 0.5427462064745825 1105 50-51 0.40254272799571567 1105 52-53 0.5397061612601277 1105 54-55 0.5610992092448299 1105 56-57 0.6472452626813592 1105 58-59 0.5869250143573694 1105 60-61 0.379205937691502 1105 62-63 0.3523588187332365 1105 64-65 0.39435231786652025 1105 66-67 0.456312451346399 1105 68-69 0.43103234993749595 1105 70-71 0.800956758071834 1105 72-73 0.7597931376603704 1105 74-75 0.6098377945612938 1105 76-77 0.6010677557426014 1105 78-79 0.43914198195565035 1105 80-81 0.697866877608913 1105 82-83 0.619232793758254 1105 84-85 0.622482711093614 1105 86-87 0.9488202650780124 1105 88-89 0.659857736027945 1105 90-91 0.7960326347739901 1105 92-93 1.2035763555248096 1105 94-95 1.1345699550989323 1105 96-97 1.5954953747555543 1105 98-99 1.5442497955740428 1105 100-101 1.8663458020256378 1106 1 0.42729238298650785 1106 2 0.4830320678865263 1106 3 0.4221174946634356 1106 4 0.4511982213988901 1106 5 0.29759749624967924 1106 6 0.353270869815276 1106 7 0.18428876983025333 1106 8 0.1990418419753226 1106 9 0.3547099529064397 1106 10-11 0.33278979286227184 1106 12-13 0.3291185201313951 1106 14-15 0.42966865387954556 1106 16-17 0.17938747961662216 1106 18-19 0.5580077345387977 1106 20-21 0.6275205738563798 1106 22-23 0.5593141257858463 1106 24-25 0.6074154237128369 1106 26-27 0.34999723689504236 1106 28-29 0.5920089005400513 1106 30-31 0.8109935568309652 1106 32-33 0.9438020253417534 1106 34-35 0.9935950044185162 1106 36-37 0.6677749830372974 1106 38-39 0.864130139051241 1106 40-41 1.0298655641329972 1106 42-43 1.0592937432719296 1106 44-45 1.184161748414411 1106 46-47 1.057645588710166 1106 48-49 1.1276670570416627 1106 50-51 1.0765607819159513 1106 52-53 1.0745258736452783 1106 54-55 1.1177214958734538 1106 56-57 0.9294409172617577 1106 58-59 1.001561369759962 1106 60-61 0.9112632191374317 1106 62-63 1.2492330954191289 1106 64-65 1.1992663252531344 1106 66-67 1.3348912837790508 1106 68-69 1.3252649576924256 1106 70-71 1.4564931929760832 1106 72-73 1.703613645062081 1106 74-75 1.8053917399567325 1106 76-77 1.9960450625485429 1106 78-79 1.80529239103695 1106 80-81 1.7515455902859038 1106 82-83 1.5593952186593825 1106 84-85 1.7882537261989278 1106 86-87 1.9273950721103859 1106 88-89 1.8988860044013798 1106 90-91 2.1412912164242037 1106 92-93 1.9452613880802332 1106 94-95 2.0755877775586526 1106 96-97 2.284392314444993 1106 98-99 2.9465332038804597 1106 100-101 3.2252756493623345 1107 1 0.04568756440366073 1107 2 -0.00553469962152775 1107 3 -0.04646399023566161 1107 4 -0.13422688927355608 1107 5 -0.07706523020541312 1107 6 0.026739462980643225 1107 7 -0.0047245809459113275 1107 8 0.06806874209562608 1107 9 0.05722008571337511 1107 10-11 0.09314939557057755 1107 12-13 -0.06310189810726996 1107 14-15 0.07229691516384662 1107 16-17 -0.07997538972954743 1107 18-19 0.3042631708237806 1107 20-21 0.2703182019424588 1107 22-23 0.18287986151912605 1107 24-25 0.27832988763771027 1107 26-27 0.14080976925967548 1107 28-29 0.26875386528449496 1107 30-31 0.2897876652023612 1107 32-33 0.2512903740738466 1107 34-35 0.10367758533497096 1107 36-37 0.13268685389803636 1107 38-39 0.3760870459072905 1107 40-41 0.22869829592217883 1107 42-43 0.3019068199464172 1107 44-45 0.36899349125720704 1107 46-47 0.40706876821172244 1107 48-49 0.3857742759609053 1107 50-51 -0.015576704992191992 1107 52-53 -0.024444687679128663 1107 54-55 0.033152952934102586 1107 56-57 0.189052969948456 1107 58-59 0.27022773273442624 1107 60-61 0.5021586828325795 1107 62-63 0.6107124657639744 1107 64-65 0.43282703838470127 1107 66-67 0.519452429087977 1107 68-69 0.5935380782076152 1107 70-71 0.6056352625216022 1107 72-73 0.6786038140005992 1107 74-75 0.7828893162594 1107 76-77 1.1901880859990364 1107 78-79 1.122121221549449 1107 80-81 1.2166170942241692 1107 82-83 1.2202274910167716 1107 84-85 1.305019092829383 1107 86-87 1.1947065006706907 1107 88-89 1.2039798569704985 1107 90-91 1.298193010933602 1107 92-93 1.0816094504850895 1107 94-95 1.385776261394831 1107 96-97 1.8528593307470373 1107 98-99 1.9862623727372117 1107 100-101 2.6279092057926263 1108 1 0.27507672014110796 1108 2 0.11573325606702412 1108 3 0.09833140658901485 1108 4 0.22926913365177626 1108 5 0.14518465774837352 1108 6 0.18697686513826994 1108 7 0.16095894622359452 1108 8 0.017939945539858115 1108 9 0.14821971901668007 1108 10-11 0.29358535719871526 1108 12-13 0.0750793768201845 1108 14-15 0.24848788718171733 1108 16-17 0.1974487696975018 1108 18-19 0.1863769735590708 1108 20-21 0.28494758928734853 1108 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-0.41872411783686303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2804.0 3 541.0 4 474.0 5 536.0 6 637.0 7 724.0 8 874.0 9 1035.0 10 1372.0 11 1740.0 12 2264.0 13 2917.0 14 3441.0 15 4177.0 16 5352.0 17 7021.0 18 5904.0 19 4195.0 20 4180.0 21 4384.0 22 4824.0 23 5300.0 24 6127.0 25 6848.0 26 7865.0 27 9213.0 28 10885.0 29 13236.0 30 16054.0 31 20334.0 32 26000.0 33 34184.0 34 46720.0 35 66504.0 36 97990.0 37 136575.0 38 110180.0 39 13578.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.753164095003136 19.521334832051153 15.31057033998511 22.4149307329606 2 14.41857206658902 19.874102031940076 42.46027923486261 23.247046666608288 3 18.859028278400253 24.846700075008247 30.20628726698557 26.087984379605928 4 12.236551043873627 16.75363181640431 38.63368411222589 32.37613302749616 5 13.153690255763767 37.6151768635124 34.882010741815606 14.349122138908221 6 30.648972198157043 36.91248151356839 18.278595993804313 14.159950294470262 7 27.592950424009977 33.003944787557515 21.257227857054314 18.145876931378197 8 25.28135446870825 35.82623991412052 20.259709573344693 18.632696043826538 9 25.68522721305928 17.308185971074018 21.197411947282134 35.80917486858456 10-11 24.096450561575693 27.296203192605766 27.972675646918717 20.63467059889982 12-13 25.01314473944246 24.631527976599813 28.6546568840149 21.700670399942826 14-15 22.002844016626558 26.04492087799898 27.095675636257567 24.856559469116895 16-17 21.047834064014047 29.396408142787557 27.77396151456549 21.78179627863291 18-19 21.023646680225447 27.751651154769185 30.393936322342164 20.830765842663197 20-21 21.801753718229715 26.870051899910024 30.568740767313702 20.75945361454656 22-23 21.419775544024677 26.834249185065723 30.368122089585537 21.37785318132406 24-25 21.108610332807658 26.951317939329517 30.67893579124115 21.261135936621674 26-27 21.039080359193672 27.1851859953843 30.44293990469628 21.33279374072575 28-29 20.905754110782368 27.21433827470754 30.651081416353865 21.228826198156227 30-31 21.165353825421526 27.034782393099814 30.564869330852034 21.234994450626623 32-33 20.923809996091176 27.115432858634712 30.39833554055552 21.562421604718594 34-35 21.284330211753108 27.161539953650294 30.309907140158998 21.244222694437596 36-37 21.293747083527766 27.02322970135324 30.30608376108259 21.376939454036396 38-39 21.051253895409797 27.30877311179261 30.31055366223206 21.329419330565532 40-41 21.273556842639415 27.199878096437967 30.262850910193823 21.263714150728795 42-43 21.138481708415274 27.079610010915665 30.169174645605327 21.612733635063723 44-45 21.189601920255726 27.353873614274754 29.907093338310908 21.54943112715861 46-47 21.352370772155194 27.51895225060938 29.420046913063548 21.70863006417188 48-49 21.36303078519493 27.545081784909808 29.445484383534076 21.64640304636119 50-51 21.41349126303653 27.77334141359336 29.22341262635434 21.589754697015774 52-53 21.480377698130326 27.95187893587505 28.990869718068446 21.576873647926178 54-55 21.368559061502832 27.758745909242048 29.143113817865746 21.72958121138937 56-57 21.3047101045159 27.989441247804812 29.055157889211834 21.65069075846745 58-59 21.43399742218438 27.70466514641643 29.199448277470943 21.66188915392825 60-61 21.5728059783104 27.655299371183816 29.301011573863118 21.470883076642668 62-63 21.33895819920687 27.784538278082344 29.31232223306981 21.564181289640977 64-65 21.366394148961763 27.637217549032044 29.211667082223357 21.78472121978283 66-67 21.24846622859555 27.808232461055148 29.10093838011868 21.842362930230625 68-69 21.18745584442949 27.733526007767733 29.238442632572433 21.840575515230345 70-71 21.257838433312166 27.65580811413182 29.102578216646606 21.983775235909413 72-73 21.313301648334424 27.608234600222946 29.066940210421638 22.011523541020992 74-75 21.239274207718562 27.800407851691883 28.95717848870315 22.003139451886405 76-77 21.243858438931483 27.684458295719537 29.086389469401208 21.985293795947772 78-79 21.300858658596614 27.66922501959783 28.987730844256465 22.042185477549086 80-81 21.249204308727485 27.86957444279736 28.921162945176963 21.96005830329819 82-83 21.2912685849448 27.947688361345797 28.853356938124193 21.907686115585214 84-85 21.30288647316579 27.71736039720904 28.83943218355607 22.140320946069103 86-87 21.22274410187687 27.69802951139524 28.943857188061866 22.135369198666023 88-89 21.42240493019878 27.507863847882486 28.851098022116478 22.218633199802255 90-91 21.21261835909416 27.582171434571283 28.984835161309608 22.220375045024944 92-93 21.38666748328842 27.36616152778264 29.128752085301972 22.118418903626967 94-95 21.102404459957143 27.410639354338667 29.137443647178657 22.349512538525534 96-97 21.03975807168905 27.411664525686657 29.022867416228387 22.525709986395906 98-99 21.036127466848644 27.485658958812703 28.831179625368552 22.6470339489701 100-101 21.275620079931297 27.14128242925818 29.19555464183285 22.387542848977677 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 831.0 1 489.0 2 129.5 3 119.5 4 117.0 5 102.5 6 152.5 7 340.0 8 580.5 9 739.0 10 735.5 11 669.5 12 709.5 13 772.0 14 822.0 15 884.5 16 926.5 17 994.0 18 1072.5 19 1187.5 20 1324.0 21 1491.5 22 1775.5 23 2172.0 24 2704.5 25 3475.5 26 4479.0 27 5581.5 28 6641.5 29 7869.0 30 9365.5 31 10893.0 32 12467.0 33 14301.0 34 16159.5 35 17892.5 36 20121.0 37 22029.0 38 22930.5 39 23422.0 40 23927.0 41 24997.5 42 25823.0 43 26172.5 44 26873.5 45 27619.0 46 27412.5 47 26981.5 48 27078.5 49 26164.5 50 24369.0 51 22715.0 52 21245.5 53 20086.0 54 18875.0 55 16998.0 56 14822.5 57 12946.5 58 11341.0 59 9668.0 60 7904.0 61 6242.0 62 4734.0 63 3486.0 64 2536.5 65 1867.0 66 1359.5 67 987.0 68 736.0 69 537.5 70 380.5 71 264.0 72 208.5 73 169.5 74 123.5 75 92.0 76 60.5 77 34.0 78 25.5 79 17.5 80 8.0 81 8.0 82 10.0 83 8.0 84 5.0 85 3.5 86 1.5 87 1.0 88 0.5 89 2.5 90 3.0 91 1.0 92 0.5 93 1.0 94 2.0 95 1.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28573799150791934 2 0.2671060694941579 3 0.25254988042090676 4 0.24177830050670096 5 0.23784812945692313 6 0.19723636194255256 7 0.1860280963561492 8 0.20102097110159786 9 0.20102097110159786 10-11 0.20051150448403401 12-13 0.19760026666938382 14-15 0.1954896192537624 16-17 0.19374287656497227 18-19 0.1945434669640011 20-21 0.1819523634156389 22-23 0.17489261171511208 24-25 0.17562042116877466 26-27 0.18784761999030558 28-29 0.20196712339135914 30-31 0.19359731467423977 32-33 0.19869198084987763 34-35 0.19447068601863485 36-37 0.17947781127318618 38-39 0.1819523634156389 40-41 0.17540207833267588 42-43 0.18653756297371296 44-45 0.1820979253063714 46-47 0.1828985157054002 48-49 0.19250560049374593 50-51 0.19963813313963896 52-53 0.21426710315825634 54-55 0.18937601984299696 56-57 0.16251985100284866 58-59 0.16608611732579517 60-61 0.17183581200972936 62-63 0.1788227827648899 64-65 0.16972516459410794 66-67 0.17227249768192687 68-69 0.17576598305950716 70-71 0.17314586902632195 72-73 0.17169025011899683 74-75 0.17532929738730962 76-77 0.18864821038933438 78-79 0.19367009561960602 80-81 0.18464525839419033 82-83 0.1828985157054002 84-85 0.17583876400487342 86-87 0.1781677542565936 88-89 0.18340798232296399 90-91 0.18369910610442902 92-93 0.18064230639904627 94-95 0.1814428967980751 96-97 0.17030741215703799 98-99 0.17452870698828082 100-101 0.1679784219053178 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 686993.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.35489192330543 #Duplication Level Percentage of deduplicated Percentage of total 1 85.68775925338801 62.85616319182492 2 9.261833692300216 13.588016190206226 3 2.3588734623277694 5.191047236694204 4 0.896376917629704 2.6301452766109037 5 0.46507272303118397 1.705767966721493 6 0.2914996133428939 1.2829753579471983 7 0.1942994271355227 0.99769694348005 8 0.14435339371910036 0.847122207602157 9 0.10330465637681413 0.6820111713325866 >10 0.5645538350662064 7.229236371298786 >50 0.025897574536848 1.2858091297738832 >100 0.005179410639264122 0.5615733147454971 >500 3.984162025938148E-4 0.2094026956568322 >1k 5.976243038907222E-4 0.9330329461052852 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2800 0.40757329405103104 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2181 0.3174704836876067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1404 0.2043688945884456 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 868 0.12634772115581963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 1.455618907325111E-4 0.0 0.0 7.278094536625555E-5 0.0 20-21 2.911237814650222E-4 0.0 0.0 2.1834283609876667E-4 0.0 22-23 2.911237814650222E-4 0.0 0.0 3.6390472683127774E-4 0.0 24-25 4.366856721975333E-4 0.0 0.0 5.822475629300444E-4 0.0 26-27 4.366856721975333E-4 0.0 0.0 8.733713443950666E-4 0.0 28-29 5.822475629300444E-4 0.0 0.0 0.0017467426887901333 0.0 30-31 7.278094536625555E-4 0.0 0.0 0.004876323339539123 0.0 32-33 7.278094536625555E-4 0.0 0.0 0.008515370607851899 0.0 34-35 7.278094536625555E-4 0.0 0.0 0.01193607504006591 0.0 36-37 7.278094536625555E-4 0.0 0.0 0.017976893505465122 0.0 38-39 7.278094536625555E-4 0.0 0.0 0.02780232112990962 0.0 40-41 7.278094536625555E-4 0.0 0.0 0.0379916534811854 0.0 42-43 7.278094536625555E-4 0.0 0.0 0.047016490706601084 0.0 44-45 7.278094536625555E-4 0.0 0.0 0.05334843295346532 0.0 46-47 7.278094536625555E-4 0.0 0.0 0.06586675555646127 0.0 48-49 7.278094536625555E-4 0.0 0.0 0.07445490710967943 0.0 50-51 7.278094536625555E-4 0.0 0.0 0.08027738273897987 0.0 52-53 8.733713443950666E-4 0.0 0.0 0.0970897811185849 0.0 54-55 0.0010189332351275776 0.0 0.0 0.12176252159774553 0.0 56-57 0.0010189332351275776 0.0 0.0 0.1633932223472437 0.0 58-59 0.0010189332351275776 0.0 0.0 0.2219091024217132 0.0 60-61 0.0010189332351275776 0.0 0.0 0.2937438954982074 0.0 62-63 0.0010189332351275776 0.0 0.0 0.3318811108701253 0.0 64-65 0.0010189332351275776 0.0 0.0 0.3823183060089404 0.0 66-67 0.0011644951258600888 0.0 0.0 0.4733672686621261 0.0 68-69 0.0011644951258600888 0.0 0.0 0.5916363048822914 0.0 70-71 0.0011644951258600888 0.0 0.0 0.7330496817289259 0.0 72-73 0.0011644951258600888 0.0 0.0 0.8888009048127128 0.0 74-75 0.0011644951258600888 0.0 0.0 1.0413497663003843 0.0 76-77 0.0011644951258600888 0.0 0.0 1.1990660749090603 0.0 78-79 0.0011644951258600888 0.0 0.0 1.3332013572190693 0.0 80-81 0.0011644951258600888 0.0 0.0 1.4349491188410946 0.0 82-83 0.0011644951258600888 0.0 0.0 1.5890991611268237 0.0 84-85 0.0011644951258600888 0.0 0.0 1.8706158578035001 0.0 86-87 0.0011644951258600888 0.0 0.0 2.2346661465255107 0.0 88-89 0.0011644951258600888 0.0 0.0 2.622734147218385 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1820 0.0 43.886208 1 GTATCAA 2840 0.0 36.99679 1 ATCAACG 3540 0.0 29.261047 3 CAACGCA 3600 0.0 28.647646 5 TCAACGC 3610 0.0 28.564121 4 TATCAAC 3630 0.0 28.412958 2 AACGCAG 3715 0.0 27.876568 6 GTGTACG 70 0.008351966 27.167652 1 ACCGTAT 35 0.0083965575 27.13992 62-63 GTACATG 3105 0.0 27.10203 1 TACATGG 3070 0.0 26.319277 2 ACATGGG 3085 0.0 25.259531 3 CGCAGAG 4230 0.0 24.596712 8 ACGCAGA 4240 0.0 24.538698 7 CATGGGG 1250 0.0 23.56948 4 GCAGAGT 4545 0.0 22.5784 9 GTGGTAT 950 0.0 22.520554 1 GAGTACT 2630 0.0 22.12697 12-13 TGGTATC 980 0.0 21.824776 2 ACCGGTG 110 0.002873135 21.59487 7 >>END_MODULE